Did you mean: aero coccaceae
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Acta Tropica Jun 2024Gram-positive catalase-negative cocci (GPCNCs) are significant components of the genital microbiota in sheep and goats. However, characterizing them can be difficult due...
Gram-positive catalase-negative cocci (GPCNCs) are significant components of the genital microbiota in sheep and goats. However, characterizing them can be difficult due to overlapping culture features and the limited information on their susceptibility to antibiotics. In this study, 97 foreskin and 13 vaginal swabs were investigated using a culturomic approach. Of 110 animals, 76 (69.09%) hosted GPCNCs, including strains from Streptococcaceae (37, 33.64%), Aerococcaceae (30, 27.27%), Enterococcaceae (6, 5.45%) and other minor species. With increasing antimicrobial resistance rates in livestock, surveillance programs are globally required, so we conducted a pilot study on GPCNCs isolated from the genital mucosa surfaces of sheep and goats using the minimal inhibitory concentration assay (MIC). Due to gaps in interpretative standard breakpoints, normalized resistance interpretation was used for setting epidemiological susceptibility cut-off values (CO). Of 57 suitable strains, the majority (80.71%) showed high CO with decrease susceptibility to at least one antimicrobial class, with 22.81% displaying multiresistant profiles. Of interest, combined resistances to beta-lactams, macrolides, lincosamides, and tetracyclines were detected in strains of Streptococcus plurianimalium. Further combinations, including resistance to beta-lactams, pleuromutilins, aminoglycosides, and lincosamides, were also recorded in both Streptococcus uberis and Enterococcus spp. strains. Being beta-lactams, macrolides, and tetracyclines the most used antibiotics in livestock worldwide, our results highlight the need for their prudent use. Collectively, our findings highlight that small ruminant genital microbiota can serve as reservoirs for opportunistic severe pathogens, often zoonotic, carrying multidrug resistances, thus standing for high risks for both animals and humans.
PubMed: 38944406
DOI: 10.1016/j.actatropica.2024.107305 -
Microbiology Spectrum Jun 2024Bacterial isolates from the human urinary microbiome have been extensively studied for their antibiotic resistance; however, little work has been done on those isolates...
UNLABELLED
Bacterial isolates from the human urinary microbiome have been extensively studied for their antibiotic resistance; however, little work has been done on those isolates that are difficult to grow . This study was designed to qualify a serum-based medium, New York City Broth III (NYCIII), and a broth microdilution method to determine the antibiotic susceptibility of previously underreported or undescribed microbes that have a difficult time growing in standard Mueller-Hinton broth. Here, we demonstrate that NYCIII microbroth dilution can be an effective method for the determination of antibiotic susceptibility of species found in the human urinary microbiome. We show that this method serves well to characterize fastidious and anaerobic urinary microbes that have no Clinical and Laboratory Standards Institute (CLSI) guidelines, including several in the families , , or . Previous studies using expanded quantitative urine culture reveal that urine samples from clinical patients are commonly polymicrobial in composition. Thus, we test whether NYCIII can serve as a viable harmonized medium, capable of supporting antibiotic susceptibility testing in a range of fastidious, non-fastidious, and anaerobic urinary microbes. We propose this methodology to be standardized comparable to CLSI standards to allow for resistance testing in uncharacterized urinary bacteria.
IMPORTANCE
Antibiotic susceptibilities of fastidious and anaerobic bacteria of the human urinary microbiome are largely underreported due to difficulty in growing them in the lab environment. The current standard medium, Muller-Hinton broth, has difficulty supporting the growth of many of these species, leaving microbiologists without a standardized method. To address this need, this study offers a methodology to survey susceptibilities in a high-throughput manner of these understudied microbes with a proposed harmonized medium, NYCIII, which is capable of supporting the growth of both fastidious and non-fastidious urinary microbes. Broader standardization of this method can allow for the development of antibiotic-resistant breakpoints of the many uncharacterized urinary microbes.
Topics: Humans; Microbial Sensitivity Tests; Anti-Bacterial Agents; Microbiota; Bacteria, Anaerobic; Urine; Urinary Tract Infections; Bacteria; Culture Media
PubMed: 38709058
DOI: 10.1128/spectrum.00314-24 -
Enfermedades Infecciosas Y... May 2024
Topics: Humans; Urinary Tract Infections; Aerococcus; Endocarditis, Bacterial; Gram-Positive Bacterial Infections; Recurrence; Male
PubMed: 38704195
DOI: 10.1016/j.eimce.2024.02.008 -
Biomedicine & Pharmacotherapy =... Jun 2024There is a growing evidence suggesting the association of vitamin D deficiency (VDD) and cognitive impairment. In this study we evaluated the possible involvement of gut...
There is a growing evidence suggesting the association of vitamin D deficiency (VDD) and cognitive impairment. In this study we evaluated the possible involvement of gut microbiota in the cognitive impairments mediated by VDD and investigated the effects of pharmacological treatment with the oxazoline derivative of the aliamide palmitoylethanolamide, 2-Pentadecyl-2-oxazoline (PEA-OXA). Mice were submitted to behavioural, biochemical and electrophysiological analysis to assess whether their vitamin D status affected cognitive performance together with gut microbiota composition. In VDD mice we found cognitive malfunctioning associated with reduced neuroplasticity, indicated by impaired long term potentiation, and neuroinflammation at the hippocampal level. Importantly, PEA-OXA counteracted the cognitive impairments and modified the biochemical and functional changes induced by VDD. Additionally, PEA-OXA treatment enhanced gut microbiota diversity, which tended to be decreased by VDD only in female mice, elevated the relative abundance of lactic and butyric acid-producing families, i.e. Aerococcaceae and Butyricicoccaceae, and reversed the VDD-induced decrease of butyrate-producing beneficial genera, such as Blautia in female mice, and Roseburia in male mice. These data provide novel insights for a better understanding of the cognitive decline induced by VDD and related gut dysbiosis and its potential therapeutic treatment.
Topics: Animals; Gastrointestinal Microbiome; Cognitive Dysfunction; Male; Female; Mice; Vitamin D Deficiency; Hippocampus; Mice, Inbred C57BL; Ethanolamines; Dysbiosis; Amides; Cognition; Disease Models, Animal
PubMed: 38670046
DOI: 10.1016/j.biopha.2024.116600 -
International Journal of Systematic and... Feb 2024Two cocci-shaped, facultatively anaerobic, Gram-positive bacteria isolated from the faeces of a pig were designated as strains YH-aer221 and YH-aer222. Analysis of the...
Two cocci-shaped, facultatively anaerobic, Gram-positive bacteria isolated from the faeces of a pig were designated as strains YH-aer221 and YH-aer222. Analysis of the 16S rRNA gene sequences revealed that the isolates were most closely related to JCM 18035 with 96.6 % similarity. The multi-locus sequence tree revealed that the isolates formed a sub-cluster adjacent to JCM 18035. The average nucleotide identity values for the isolates and their most closely related strains were 71.8 and 71.7 %, respectively; and the digital DNA-DNA hybridization values for the isolates and their most closely related strains were 25.6 and 25.5 %, respectively. The main fatty acids were Cω9, C and C. The cell wall contained the -diaminopimelic acid-based peptidoglycan. The two isolates shared the same metabolic pathways. Isolates YH-aer221 and YH-aer222 harboured the same CRISPR array with 33 and 46 spacers, respectively. Single-genome vs. metagenome analysis showed that the genomes of the isolates were not found in the available metagenome database. Given their chemotaxonomic, phenotypic and phylogenetic properties, YH-aer221 (= KCTC 25571=JCM 35699) and YH-aer222 (=KCTC 25573=JCM 35700) represent a novel taxon. The name sp. nov. is proposed.
Topics: Swine; Animals; Aerococcus; Anaerobiosis; Base Composition; Fatty Acids; Phylogeny; RNA, Ribosomal, 16S; Sequence Analysis, DNA; DNA, Bacterial; Bacterial Typing Techniques; Bacteria, Anaerobic; Feces
PubMed: 38415779
DOI: 10.1099/ijsem.0.006284 -
The American Journal of Medicine May 2024
Topics: Humans; Aerococcus; Urinary Tract Infections; Gram-Positive Bacterial Infections; Anti-Bacterial Agents; Male; Female; Communicable Diseases, Emerging
PubMed: 38320725
DOI: 10.1016/j.amjmed.2024.01.029 -
BMJ Case Reports Jan 2024is a type of Streptococci and is a rare cause of infectious endocarditis. The progression and outcomes of infectious intracranial aneurysms (IIAs) associated with this...
is a type of Streptococci and is a rare cause of infectious endocarditis. The progression and outcomes of infectious intracranial aneurysms (IIAs) associated with this species are unknown due to a limited number of reported cases. A woman in her 20s with a sudden headache had a subarachnoid haemorrhage on a head CT scan. Cerebral angiography showed an aneurysm on the right middle cerebral artery. She was diagnosed with infectious endocarditis caused by and underwent parent artery occlusion. Despite initiating targeted antibiotic therapy, a new IIA developed and ruptured 14 days postadmission. A second parent artery occlusion was performed on the new IIA. Following 6 weeks of continued antibiotic therapy, she underwent mitral valve repair and was discharged with no neurological symptoms. Endocarditis caused by can lead to the delayed formation of an IIA. Endovascular treatment was effective for repeated ruptured IIAs.
Topics: Female; Humans; Abiotrophia; Aneurysm, Infected; Aneurysm, Ruptured; Anti-Bacterial Agents; Endocarditis; Endocarditis, Bacterial; Intracranial Aneurysm; Adult
PubMed: 38290982
DOI: 10.1136/bcr-2023-258922 -
Journal of Biotechnology Feb 2024l-Lactate oxidase has important applications in biosensing and finds increased use in biocatalysis. The enzyme has been characterized well, yet its immobilization has...
l-Lactate oxidase has important applications in biosensing and finds increased use in biocatalysis. The enzyme has been characterized well, yet its immobilization has not been explored in depth. Here, we studied immobilization of Aerococcus viridansl-lactate oxidase on porous carriers of variable matrix material (polymethacrylate, polyurethane, agarose) and surface functional group (amine, Ni-loaded nitrilotriacetic acid (NiNTA), epoxide). Carrier activity (A) and immobilized enzyme effectiveness (ɳ) were evaluated in dependence of protein loading. Results show that efficient immobilization (A: up to 1450 U/g carrier; ɳ: up to 65%) requires a hydrophilic carrier (agarose) equipped with amine groups. The value of ɳ declines sharply as A increases, probably due to transition into diffusional regime. Untagged l-lactate oxidase binds to NiNTA carrier similarly as N-terminally His-tagged enzyme. Lixiviation studies reveal quasi-irreversible enzyme adsorption on NiNTA carrier while partial release of activity (≤ 25%) is shown from amine carrier. The desorbed enzyme exhibits the same specific activity as the original l-lactate oxidase. Collectively, our study identifies basic requirements of l-lactate oxidase immobilization on solid carrier and highlights the role of ionic interactions in enzyme-surface adsorption.
Topics: Aerococcus; Sepharose; Mixed Function Oxygenases; Enzymes, Immobilized; Amines
PubMed: 38280467
DOI: 10.1016/j.jbiotec.2024.01.011 -
International Journal of Molecular... Nov 2023() is a swine pathogen that can cause sepsis, meningitis, endocarditis, and other infectious diseases; it is also a zoonotic pathogen that has caused a global surge in...
() is a swine pathogen that can cause sepsis, meningitis, endocarditis, and other infectious diseases; it is also a zoonotic pathogen that has caused a global surge in fatal human infections. The widespread prevalence of multidrug-resistant strains and the decline in novel antibiotic candidates have necessitated the development of alternative antimicrobial agents. In this study, AVPL, the () phage lysin, was found to exhibit efficient bactericidal activity and broad lytic activity against multiple serotypes of . A final concentration of 300 μg/mL AVPL reduced counts by 4-4.5 log10 within 1 h in vitro. Importantly, AVPL effectively inhibited 48 h biofilm formation and disrupted preformed biofilms. In a mouse model, 300 μg/mouse AVPL protected 100% of mice from infection following the administration of lethal doses of multidrug-resistant type 2 (SS2) strain SC19, reduced the bacterial load in different organs, and effectively alleviated inflammation and histopathological damage in infected mice. These data suggest that AVPL is a valuable candidate antimicrobial agent for treating infections.
Topics: Animals; Swine; Humans; Mice; Bacteriophages; Aerococcus; Streptococcus suis; Streptococcal Infections; Bacteremia; Disease Models, Animal
PubMed: 38068990
DOI: 10.3390/ijms242316670 -
International Journal of Systematic and... Nov 2023A non-spore-forming, Gram-stain-positive, short rod-shaped strain, designated SJQ22, was isolated from a paddy soil sample collected in Shanghai, PR China. A comparative...
A non-spore-forming, Gram-stain-positive, short rod-shaped strain, designated SJQ22, was isolated from a paddy soil sample collected in Shanghai, PR China. A comparative analysis of 16S rRNA gene sequences showed that strain SJQ22 fell within the genus , forming a clear cluster with the type strains of (98.6 % sequence similarity) and (98.5 % sequence similarity). Strain SJQ22 grew at 30-45 °C (optimum, 30 °C), pH 6.0-8.0 (optimum, pH 7.0) and with a NaCl concentration of 0-4 % (optimum, 1 %). Cells were negative for oxidase and catalase activity. Chemotaxonomic analysis showed that strain SJQ22 possessed C and C ω9 as the predominant fatty acids. The DNA G + C content was 39.0 mol%. Strain SJQ22 exhibited DNA-DNA relatedness levels of 13±2 % with ATCC 11563 and 9±2 % with IFO 12173. Based on the data obtained, strain SJQ22 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is SJQ22 (=JCM 33111=CCTCC AB 2018283).
Topics: Fatty Acids; Soil Microbiology; Aerococcus; RNA, Ribosomal, 16S; DNA, Bacterial; Nucleic Acid Hybridization; Base Composition; China; Phylogeny; Bacterial Typing Techniques; Sequence Analysis, DNA
PubMed: 37909297
DOI: 10.1099/ijsem.0.006069