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International Journal of Systematic and... Mar 2022An anaerobic bacterial strain, designated AMB_01, recovered from mesophilic propionate enrichment of a high-ammonia biogas digester, was characterised using phenotypic...
An anaerobic bacterial strain, designated AMB_01, recovered from mesophilic propionate enrichment of a high-ammonia biogas digester, was characterised using phenotypic and molecular taxonomic methods. Cells of AMB_01 are coccus-shaped and often occur arranged as diplococci or sarcina. Growth occurred at 20-45 °C, initial pH 5.5-8.5 and with up to 0.7 M NHCl, with optimum growth at 37-42 °C and pH 8.0. AMB_01 achieved high cell density and highest acetate production when grown on carbohydrates, including monomers, disaccharides and polysaccharides, such as glucose, maltose, cellobiose and starch. The strain was also able to use amino acids and some organic acids and alcoholic compounds for growth. Acetate was formed as the main product and yeast was not required for growth. The major cellular fatty acids were summed feature 4 (iso-CI and/or anteiso-CB), Cω7, C, C and summed feature 3 (Cω7 and/or iso-C 2OH). The highest 16S rRNA gene sequence similarity found was with (96.6 %), within the family , phylum Bacillota (Firmicutes). The genomic DNA G+C content was 29.0 mol%. An almost complete set of genes for the acetyl-CoA pathway was found. Genome comparisons between AMB_01 and close relatives showed highest digital DNA-DNA hybridisation to (23 %), highest average nucleotide identity with genome nucleotide and amino acid sequences to (72 and 73 %, respectively) and highest average nucleotide identity (87 %) with , indicating that AMB_01 represents a novel species. Analysis of genomic, chemotaxonomic, biochemical and physiological data confirmed that strain AMB_01 represents a novel species, for which the name sp. nov. is proposed. The type strain is AMB_01 (=DSM 110247=JCM 39107 ).
Topics: Acetates; Ammonium Compounds; Bacterial Typing Techniques; Base Composition; Biofuels; DNA, Bacterial; Fatty Acids; Phylogeny; RNA, Ribosomal, 16S; Sequence Analysis, DNA
PubMed: 35312473
DOI: 10.1099/ijsem.0.005328 -
Infection and Drug Resistance 2022is a Gram-positive anaerobic coccus, which forms part of the normal flora and the human commensals of the skin, genitourinary system, and gut. It can cause...
is a Gram-positive anaerobic coccus, which forms part of the normal flora and the human commensals of the skin, genitourinary system, and gut. It can cause opportunistic infections in immunocompromised patients and is frequently isolated as part of polymicrobial spectra. Severe monomicrobial infections caused by the genus rarely occur. In this study, we report on septic shock, renal abscess, and bacteremia due to in a woman with nephrosis and diabetes mellitus. To the best of our knowledge, this report is the first to describe isolated from both renal abscess and blood cultures purely. The underlying diseases of the host and the removal of the double J tube were significant predisposing factors in this infection.
PubMed: 35281574
DOI: 10.2147/IDR.S353966 -
Current Research in Microbial Sciences 2022() is a facultative anaerobic, coccus-shaped halophilic lactic acid-producing bacterium previously detected and cultured in various salty foods and credited for...
() is a facultative anaerobic, coccus-shaped halophilic lactic acid-producing bacterium previously detected and cultured in various salty foods and credited for beneficial effects on human health. In this study, we investigated the presence of in human samples using a polyphasic approach including scanning electron microscopy, molecular biology methods and microbial culture. This unique investigation yielded the unprecedented presence of in human feces samples, thus enriching the repertoire of halophilic microorganisms colonizing the human gastrointestinal tract with the isolation and culture of for the first time in humans. Using the E-test strips, the MIC was assessed for T. strain CSURQ6002: rifampicin (MIC at 0.002 μg/mL), benzylpenicillin (MIC at 0.094 μg/mL), amoxicillin (MIC at 0.5 μg/mL), erythromycin (MIC at 2 μg/mL), clindamycin (MIC at 4 μg/mL), and vancomycin (MIC at 8 μg/mL). However, this strain showed a MIC up to 256 μg/mL for ciprofloxacin, fosfomycin, doxycyclin, imipenem, and colistin. profiling derived from whole genome sequencing (NCBI accession number: PRJNA780809), was confirmed. This discovery suggested that was part of the human digestive microbiota and that its potential role on human health should be considered.
PubMed: 35243447
DOI: 10.1016/j.crmicr.2022.100112 -
Cureus Jan 2022is a gram-positive anaerobic coccus found on the skin, vagina, and gut, where it acts as an opportunistic pathogen or as part of polymicrobial infections of chronic...
is a gram-positive anaerobic coccus found on the skin, vagina, and gut, where it acts as an opportunistic pathogen or as part of polymicrobial infections of chronic wounds or diabetic ulcers We present a case of a 68-year-old woman who was diagnosed with a late prosthetic hip arthroplasty infection caused by and isolated from sonication fluid cultures. Despite the fact that evidence is scarce, its role and pathogenicity in more severe infections should not be underestimated.
PubMed: 35198324
DOI: 10.7759/cureus.21419 -
IDCases 2022
PubMed: 35127449
DOI: 10.1016/j.idcr.2022.e01417 -
Germs Dec 2021Emphysematous endocarditis is caused by the gas-forming organisms , , species, and . We report the first case of emphysematous endocarditis caused by .
INTRODUCTION
Emphysematous endocarditis is caused by the gas-forming organisms , , species, and . We report the first case of emphysematous endocarditis caused by .
CASE REPORT
An 82-year-old man presented with fever and rapidly progressive shortness of breath. He was found to be in atrial fibrillation with rapid ventricular rates. Two-dimensional transthoracic echocardiography demonstrated severe mitral regurgitation. Subsequent two- and three-dimensional transesophageal echocardiogram revealed a large, highly mobile vegetation on the atrial surface of the anterior mitral leaflet with aneurysmal destruction of the lateral scallop requiring mitral valve replacement. Sequencing of the vegetation revealed an anaerobic gram-positive coccus that, in rare cases, produces gas using a heme-dependent catalase. Histopathological analysis of the infected valve suggested interstitial gas accumulation, leading to the diagnosis of emphysematous endocarditis.
CONCLUSIONS
associated emphysematous endocarditis should be included in the differential diagnosis of valvular vegetation in patients with a rapidly progressing clinical course. When possible, histopathological analysis should be used alongside other imaging techniques to confirm the diagnosis of emphysematous endocarditis. This case also highlights the importance of collecting blood cultures prior to initiating antibiotic treatment.
PubMed: 35096679
DOI: 10.18683/germs.2021.1297 -
Archives of Microbiology Dec 2021Native Shewanella sp. RCRI7 is recently counted as an operative bacterium in the uranium bio-reduction. The aim of this study was to investigate the effects of uranium...
Native Shewanella sp. RCRI7 is recently counted as an operative bacterium in the uranium bio-reduction. The aim of this study was to investigate the effects of uranium tolerance on the morphology and population of RCRI7, following its potential removal capacity in different time intervals. In this research, the bacterial growth and uranium removal kinetic were evaluated in aerobic TSB medium, uranium-reducing condition (URC), aerobic uranium-containing (AUC) and anaerobic uranium-free (AUF) solution, following evaluations of omcAB gene expressions. In addition, spectrophotometry analyses were performed in URC confirming the bio-reduction mechanism. It was found that the bacteria can grow efficiently in the presence of 0.5 mM uranium anaerobically, unlike AUC and AUF solutions. Since the bacterium's adsorption capacity is quickly saturated, it can be deduced that uranium reduction should be dominant as incubation times proceed up to 84 h in URC. In 92 h incubation, the adsorbed uranium containing unreduced and reduced (U (IV) monomeric), was released to the solution due to either increased pH or bacterial death. In AUC and AUF, improper conditions lead to the reduced bacterial size (coccus-shape formation) and increased bacterial aggregations; however, membrane vesicles produced by the bacteria avoid the uranium incrustation in AUC. In overall, this study implies that Shewanella sp. RCRI7 are well tolerated by uranium under anaerobic conditions and the amount of regenerated uranium increases over time in the reduced form.
Topics: Adsorption; Biodegradation, Environmental; Kinetics; Oxidation-Reduction; Shewanella; Uranium
PubMed: 34958431
DOI: 10.1007/s00203-021-02716-6 -
Archives of Microbiology Dec 2021A Gram-stain-positive, coccus-shaped, facultatively anaerobic, non-motile bacterial strain, designated YIM S02567, was isolated from a forest soil sample collected from...
Propioniciclava soli sp. nov., isolated from forest soil, Yunnan, China, and reclassification of the genus Brevilactibacter into the genus Propioniciclava, and Brevilactibacter sinopodophylli, Brevilactibacter flavus, and Brevilactibacter coleopterorum as Propioniciclava sinopodophylli comb. nov.,...
A Gram-stain-positive, coccus-shaped, facultatively anaerobic, non-motile bacterial strain, designated YIM S02567, was isolated from a forest soil sample collected from Gejiu City, Yunnan Province, southwest PR China. Growth was observed at 10-45 °C, at pH 6.0-9.5, in the presence of up to 4.0% (w/v) NaCl on R2A medium. The results of 16S rRNA gene sequence similarity analysis showed that strain YIM S02567 was most closely related to the type strain of Brevilactibacter sinopodophylli (95.4%) and Propioniciclava tarda (94.7%), and phylogenetic analysis based on genome data showed that strain YIM S02567 should be assigned to the genus Propioniciclava. The cell-wall diamino acid was meso-diaminopimelic acid. The major cellular fatty acids were identified as anteiso-C and C, and the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, and two unidentified glycolipids. The predominant menaquinone was MK-9(H). The genomic DNA G + C content was 71.2 mol%. Based on the polyphasic taxonomic evidence, strain YIM S02567 is assigned to a novel member of the genus Propioniciclava, for which the name Propioniciclava soli sp. nov., (type strain YIM S02567 = CCTCC AB 2020128 = CGMCC 1.18504 = KCTC 49478) is proposed. Furthermore, we propose the reclassification of Brevilactibacter as Propioniciclava gen. nov.
Topics: China; Forests; Phylogeny; Propionibacteriaceae; RNA, Ribosomal, 16S; Soil
PubMed: 34928401
DOI: 10.1007/s00203-021-02621-y -
Revista Argentina de Microbiologia 2022Dolosigranulum pigrum is a gram-positive, facultatively anaerobic coccus, which is part of the oral and upper respiratory tract microbiota. Although reports of...
Dolosigranulum pigrum is a gram-positive, facultatively anaerobic coccus, which is part of the oral and upper respiratory tract microbiota. Although reports of infections by this microorganism are scarce, it has been associated with a wide spectrum of infectious diseases. The case of an elderly man with a lower corneal abscess, in which Dolosigranulum pigrum was isolated, is described. The microorganism was identified by mass spectrometry (MALDI-TOF MS) and by the sequencing of the 16S rRNA gene. Furthermore, the presumptive identification of the causative agent was achieved by using key phenotypic tests such as the cluster arrangement in Gram stain, the negative catalase test, the production of pyrrolidonyl arylamidase and leucine aminopeptidase activity, the growth in 6.5% NaCl and esculin hydrolysis. The data from the literature (and the present case) support the association of the microorganism with ocular infections, which often take a destructive course, mainly in elderly patients.
Topics: Abscess; Aged; Carnobacteriaceae; Gram-Positive Bacterial Infections; Gram-Positive Cocci; Humans; Male; RNA, Ribosomal, 16S
PubMed: 34895780
DOI: 10.1016/j.ram.2021.10.002 -
BMC Ophthalmology Nov 2021Parvimonas micra is known as a causative agent of chronic periodontal disease. This Gram-positive obligate anaerobic coccus was cultured from the ocular surface of...
BACKGROUND
Parvimonas micra is known as a causative agent of chronic periodontal disease. This Gram-positive obligate anaerobic coccus was cultured from the ocular surface of blowout fracture-related orbital cellulitis progressing to panophthalmitis.
CASE PRESENTATION
The patient was a woman in her fifties who had panic disorder and subsequently was a victim of domestic violence. These factors led to delayed consultation. At the initial visit to an ophthalmologist, the ocular surface of the right eye was covered with pus. Swelling of the upper and lower eyelids prevented the eyelid from closing and exophthalmos, severe corneal ulcer, panophthalmitis, and no light perception were observed. Head computed tomography revealed an old blowout fracture and chronic sinusitis with orbital cellulitis. P. micra were isolated from culture of pus samples from the sinus and from the ocular surface.
CONCLUSIONS
There is a possibility that P. micra invaded the orbit via the fragile bony site and caused orbital cellulitis, severe corneal ulcer, and panophthalmitis that required enucleation. In cases of coexisting old blowout fracture and chronic sinusitis, the chronic sinusitis should be treated as quickly as possible.
Topics: Female; Firmicutes; Humans; Orbit; Orbital Cellulitis; Orbital Fractures; Panophthalmitis
PubMed: 34758776
DOI: 10.1186/s12886-021-02153-5