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Journal of Hazardous Materials Feb 2023Phosphine (PH) is an ideal fumigant alternative on methyl bromide (MB) as MB has been classified as an ozone-depleting substance. However, several challenges limit its...
Phosphine gas in the dark induces severe phytotoxicity in Arabidopsis thaliana by increasing a hypoxia stress response and disrupting the energy metabolism: Transcriptomic approaches.
Phosphine (PH) is an ideal fumigant alternative on methyl bromide (MB) as MB has been classified as an ozone-depleting substance. However, several challenges limit its efficient use in crop production, including the emergence of PH-resistant insect pests and the incidence of phytotoxic effects on nursery plants. Therefore, this study aims to elucidate the mechanism underlying PH phytotoxicity in plants using transcriptomic techniques. Fumigation with 2 g/m PH induced phytotoxic effects in A. thaliana, as evidenced by a decrease in growth and vegetation indices compared to the control group. Transcriptomic analysis revealed that PH fumigation phytotoxicity responses in A. thaliana involve genes related to hypoxia stress and energy metabolism. Additionally, pretreatment with ethylene induced pre-adaptation to hypoxia under light conditions during fumigation effectively suppressed the phytotoxic effects of PH in A. thaliana by increasing the expression of hypoxia-adaptive genes. Moreover, the phytotoxicity of PH was also confirmed in pumpkin (Cucurbita moschata Duch.), and was dependent on light. Overall, our findings showed that fumigation under light conditions and ethylene pretreatment could be used to minimize PH-induced phytotoxic effects in plants.
Topics: Arabidopsis; Transcriptome; Phosphines; Cucurbita; Hypoxia; Energy Metabolism; Ethylenes
PubMed: 36241498
DOI: 10.1016/j.jhazmat.2022.130141 -
Foods (Basel, Switzerland) Aug 2022The new scenario for global food production and supply is decidedly complex given the current forecast of an increase in food fragility due to international tensions. In...
The new scenario for global food production and supply is decidedly complex given the current forecast of an increase in food fragility due to international tensions. In this period, exports from other parts of the world require different routes and treatments to preserve the food quality and integrity. Fumigation is a procedure used for the killing, removal, or rendering infertile of pests, with serious dangers to human health. The most-used fumigants are methyl bromide and ethylene dibromide. It is important to bear in mind that the soil may contain bromide ions naturally or from anthropogenic source (fertilizers and pesticides that contain bromide or previous fumigations). Different methods (titrimetric, spectrophotometric, and fluorometric approaches) are available to rapidly determine the amount of bromide ion on site in the containers, but these are non-specific and with high limits of quantification. The increasing interest in healthy food, without xenobiotic residues, requires the use of more sensitive, specific, and accurate analytical methods. In order to help give an overview of the bromide ion scenario, a new, fast method was developed and validated according to SANTE 11312/2021. It involves the determination of bromide ion in cereals and legumes through ion chromatography-Q-Orbitrap. The extraction was performed by the QuPPe method, but some modifications were applied based on the matrix. The method described here was validated at four different levels. Recoveries were satisfactory and the mean values ranged between 99 and 106%, with a relative standard deviation lower than 3%. The linearity in the matrix was evaluated to be between 0.010 and 2.5 mg kg, with a coefficient of determination (R) of 0.9962. Finally, the proposed method was applied to different cereals and legumes (rice, wheat, beans, lentils pearled barley, and spelt) and tested with satisfactory results in EUPT-SMR16 organized by EURL.
PubMed: 36010386
DOI: 10.3390/foods11162385 -
Journal of Hazardous Materials Oct 2022Fusarium and phenolic acids in apple replant soil have deleterious effects on soil, which affects the growth of young replanted apple trees. Here, we studied the effects...
Fusarium and phenolic acids in apple replant soil have deleterious effects on soil, which affects the growth of young replanted apple trees. Here, we studied the effects of different chemical fumigants (metham sodium, dazomet, calcium cyanamide, 1,3-dichloropropene, and methyl bromide) on Fusarium and phenolic acids in soil. The chemical fumigants disturbed the apple replant soil microbial community to different degrees in the order from highest to the lowest as methyl bromide > 1,3-dichloropropene > dazomet > metham sodium > calcium cyanamide. Compared with the control, the total numbers of Operational Taxonomic Unit (OTU) were 104.63 % and 9.38 % lower in the methyl bromide and calcium cyanamide treatments, respectively while the average contents of Fusarium were 88.04 % and 59.18% lower in these treatments, respectively. Higher disturbance degrees resulted in a slower recovery rate of the soil microbial community, which facilitated the transformation of the soil into a disease-suppressing state. During the recovery process, the roots recruited Streptomyces OTU2796 and Bacillus OTU2243, which alleviated Fusarium-induced stress via the synthesis of polyketones and macrolides. The roots also recruited Sphingomonas OTU3488, OTU5572, and OTU8147, which alleviated phenolic acid-induced stress through the degradation of benzoate and polycyclic aromatic hydrocarbons.
Topics: Allyl Compounds; Cyanamide; Fusarium; Hydrocarbons, Brominated; Hydrocarbons, Chlorinated; Hydroxybenzoates; Macrolides; Malus; Microbiota; Pesticides; Polycyclic Aromatic Hydrocarbons; Soil; Thiadiazines; Thiocarbamates
PubMed: 36007363
DOI: 10.1016/j.jhazmat.2022.129786 -
BioMed Research International 2022Forced air heat treatment could induce defenses to protect fruit from pathogen attacks and has been applied as an alternative to methyl bromide for phytosanitary...
Forced air heat treatment could induce defenses to protect fruit from pathogen attacks and has been applied as an alternative to methyl bromide for phytosanitary treatment before exportation. However, few studies were reported on the regulation mechanism of antifungal effect and delayed physiological disorders of papaya by heat treatment. Therefore, we aim to explore the fruit's resistance to pathogens and the inhibition of physiological disorders by metabolomic profiling. In our study, papaya fruits were treated with 47.2°C for 30, 60, and 90 min by forced hot air treatment. The disease resistance against , quality parameters, and metabolites of papaya fruits were measured during 10 days of storage after heat treatment by metabolomic profiling. Papaya fruits after 30 and 60 min heat treatment had higher firmness, a delayed degreening and yellowing (lower a value) process, and a higher lightness (L) and hue angle (h) during storage. Heat treatment also delayed ripening, inhibiting the growth of and softening of papaya. Metabolites and enzymes inhibited ROS scavenging, depressed ABA-regulated respiratory, and activated phenylpropanoid metabolism. Our study provides a broad picture of fruit resistance to pathogens and the inhibition of physiological disorders by metabolomic profiling, which is induced by heat treatment.
Topics: Carica; Colletotrichum; Disease Resistance; Vegetables
PubMed: 35968234
DOI: 10.1155/2022/5162845 -
Problemy Sotsial'noi Gigieny,... Jul 2022The use of various harmful substances for fumigation and preservation of goods during their transportation in containers to preserve it for longer time are widely used...
The use of various harmful substances for fumigation and preservation of goods during their transportation in containers to preserve it for longer time are widely used in sea transportations. In the context of increasing demand for carriage of goods in containers by sea, issue of customs officials safety, who are, as part of their duty customs inspection, the first to open container treated with fumigants, is becoming increasingly relevant. The results of analysis established absence of clear legal regulation of issues related to ensuring security of customs officials authorized to conduct customs inspections, both at international and national levels. In this regard, detailed analysis of main fumigants that customs inspector may encounter in his professional work is presented in this article. The risks of negative effect of fumigants on inspector health are studied and appropriate conclusions and recommendations regarding the legal and technical regulation of this question are formulated.
Topics: Fumigation; Pesticides
PubMed: 35960289
DOI: 10.32687/0869-866X-2022-30-4-592-599 -
MBio Aug 2022This study examined the viability and diversity of fungi harbored in imported wooden handicraft products sold in six retail stores in Florida, United States. Despite...
This study examined the viability and diversity of fungi harbored in imported wooden handicraft products sold in six retail stores in Florida, United States. Despite being subjected to trade regulations that require various sterilization/fumigation protocols, our study demonstrates high survival and diversity of fungi in wood products originating from at least seven countries on three continents. Among these fungi were nonendemic plant and human pathogens, as well as mycotoxin producers. Several products that are sold for use in food preparation and consumption harbored a novel (to North America) plant and human pathogen, . In addition, a high number of species isolated were thermophilic and included halophilic species, suggesting adaptability and selection through current wood treatment protocols that utilize heat and/or fumigation with methyl-bromide. This research suggests that current federal guidelines for imports of wooden goods are not sufficient to avoid the transit of potential live pathogens and demonstrates the need to increase safeguards at both points of origin and entry for biosecurity against introduction from invasive fungal species in wood products. Future import regulations should consider living fungi, their tolerance to extreme conditions, and their potential survival in solid substrates. Mitigation efforts may require additional steps such as more stringent fumigation and/or sterilization strategies and limiting use of wood that has not been processed to remove bark and decay. This study, the first of its kind, demonstrates the risk of importation of nonendemic foreign fungi on wooden handicrafts into the United States despite the application of sanitation protocols. Previous risk assessments of imported wood products have focused on potential for introduction of invasive arthropods (and their fungal symbionts) or have focused on other classes of wood products (timber, wooden furniture, garden products, etc.). Little to no attention has been paid to wooden handicrafts and the fungal pathogens (of plants and humans) they may carry. Due to the large size and diversity of this market, the risk for introduction of potentially dangerous pathogens is significant as illustrated by the results of this study.
Topics: Florida; Fungi; Humans; North America; United States; Wood
PubMed: 35766379
DOI: 10.1128/mbio.01075-22 -
Appetite Aug 2022X-ray irradiation is becoming a mainstream phytosanitary food treatment that is compatible with solving sustainability challenges. However, understanding how consumers...
X-ray irradiation is becoming a mainstream phytosanitary food treatment that is compatible with solving sustainability challenges. However, understanding how consumers respond to X-ray irradiation is still underdeveloped in research. In two studies, the current research sheds light on the acceptance of X-ray irradiation by New Zealand consumers, compared with an existing treatment, methyl bromide. Study 1 used focus groups to provide insights into barriers and opportunities of X-ray irradiation perceived by participants who accept, are passive towards, or reject irradiation. Acceptors of X-ray highlighted familiarity of use, non-residue/lack of chemicals, and environmentally friendly as positive factors, whereas danger of radiation and long-term health concerns were strong themes for rejection. Subsequently, study 2 extended this to provide a snapshot of measures representative of such themes to understand current attitudes towards X-ray irradiation itself, and as a replacement for methyl bromide usage. The results of study 2 indicated that when provided with information on X-ray in isolation, under half the respondents (39%) indicated a willingness to eat foods treated with X-ray. However, when forced to choose between food that had been treated with either X-ray or methyl bromide, the majority of respondents (84%) chose irradiation. Overall, the studies present an overview that whilst consumers may be apprehensive or passive about acceptance of X-ray irradiation (study 1), they overwhelmingly accept X-ray irradiation over the current alternative in New Zealand, methyl bromide (study 2). This was voiced by participants as accepting 'the lesser of two evils'.
PubMed: 35598719
DOI: 10.1016/j.appet.2022.106085 -
Pest Management Science Aug 2022The effectiveness of metam potassium, 1,3-dichloropropene, chloropicrin, and different ratios of 1,3-dichloropropene and chloropicrin on the reduction of natural and... (Meta-Analysis)
Meta-Analysis
BACKGROUND
The effectiveness of metam potassium, 1,3-dichloropropene, chloropicrin, and different ratios of 1,3-dichloropropene and chloropicrin on the reduction of natural and artificial inoculum of Macrophomina phaseolina were investigated in laboratory and field experiments. Additionally, a multivariate meta-analysis with data from six field trials conducted in Florida from 2012 to 2018 was performed.
RESULTS
In small-plot field experiments using drip stakes, the highest rate (468 L ha ) of metam potassium was most effective in controlling M. phaseolina in infected crowns buried at 15.2 cm from the point of fumigant injection, whereas none of the rates was able to reduce inoculum buried at 30.5 cm. In closed-container experiments, use of the highest rate of 1,3-dichloropropene (168 kg ha ) resulted in the highest level of pathogen control. Different rates of chloropicrin also reduced inoculum when compared to the non-treated control. 1,3-dichloropropene + chloropicrin at different ratios were also highly effective in controlling M. phaseolina. Results from the meta-analysis of open-field experiments indicated that metam potassium and 1,3-dichloropropene + chloropicrin (63:35, v:v) treatments were significantly more effective in reducing M. phaseolina than the 1,3-dichloropropene + chloropicrin (39:60, v:v) treatment; however, metam potassium was not as effective at the side of the beds.
CONCLUSION
1,3-dichloropropene alone and in mixture with chloropicrin were more effective in reducing inoculum of M. phaseolina than chloropicrin alone, indicating the fungicidal efficacy of 1,3-dichloropropene. Formulation with higher 1,3-dichloropropene concentration performed better than the formulation with higher chloropicrin concentration in field trials. Metam potassium was effective when applied at the highest rate, but with limited lateral movement perpendicular to the drip irrigation line. © 2022 Society of Chemical Industry.
Topics: Allyl Compounds; Ascomycota; Fragaria; Fumigation; Fungicides, Industrial; Hydrocarbons, Chlorinated; Pesticides; Potassium
PubMed: 35544358
DOI: 10.1002/ps.6982 -
Occupational and Environmental Medicine Jun 2022To assess associations between occupational exposures to pesticides and other chemicals and motor neuron disease (MND).
OBJECTIVES
To assess associations between occupational exposures to pesticides and other chemicals and motor neuron disease (MND).
METHODS
A population-based case-control study that included 319 MND cases (64% male/36% female) recruited through the New Zealand MND Association complemented with hospital discharge data, and 604 controls identified from the Electoral Roll. For each job held, a questionnaire collected information on 11 exposure categories (dust, fibres, tobacco smoke, fumes, gas, fumigants, oils/solvents, acids/alkalis, pesticides, other chemicals and animals/animal products). ORs were estimated using logistic regression adjusting for age, sex, ethnicity, socioeconomic status, education, smoking, alcohol consumption, physical activities, head/spine injury and other occupational exposures.
RESULTS
Two exposure categories were associated with increased MND risks: pesticides (OR 1.70, 95% CI 1.17 to 2.48) and fumigants (OR 3.98, 95% CI 1.81 to 8.76), with risks increasing with longer exposure duration (p<0.01). Associations were also observed for: methyl bromide (OR 5.28, 95% CI 1.63 to 17.15), organochlorine insecticides (OR 3.28, 95% CI 1.18 to 9.07), organophosphate insecticides (OR 3.11, 95% CI 1.40 to 6.94), pyrethroid insecticides (OR 6.38, 95% CI 1.13 to 35.96), inorganic (copper) fungicides (OR 4.66, 95% CI 1.53 to 14.19), petrol/diesel fuel (OR 2.24, 95% CI 1.27 to 3.93) and unspecified solvents (OR 1.91, 95% CI 1.22 to 2.99). In women, exposure to textile fibres (OR 2.49, 95% CI 1.13 to 5.50), disinfectants (OR 9.66, 95% CI 1.29 to 72.44) and cleaning products (OR 3.53, 95% CI 1.64 to 7.59) were also associated with MND; this was not observed in men (OR 0.80, 95% CI 0.44 to 1.48; OR 0.72, 95% CI 0.29 to 1.84; OR 0.57, 95% CI 0.21 to 1.56, respectively).
CONCLUSIONS
This study adds to the evidence that pesticides, especially insecticides, fungicides, and fumigants, are risk factors for MND.
Topics: Case-Control Studies; Female; Fungicides, Industrial; Humans; Insecticides; Male; Motor Neuron Disease; New Zealand; Occupational Exposure; Pesticides; Risk Factors; Solvents
PubMed: 35318275
DOI: 10.1136/oemed-2021-108056 -
Journal of Economic Entomology Apr 2022The ham mite, Tyrophagus putrescentiae (Schrank) (Sarcoptiformes: Acaridae), is a common pest infesting several stored products, particularly the aged hams. In this...
The ham mite, Tyrophagus putrescentiae (Schrank) (Sarcoptiformes: Acaridae), is a common pest infesting several stored products, particularly the aged hams. In this study, we reported the efficacy of nitric oxide (NO) fumigation, a recently discovered fumigation treatment under the ultra-low oxygen environment, at various concentrations and time under the laboratory conditions at 25°C against different mite stages on both dietary media and ham meat. Our results showed that NO fumigation was effective against all mite stages and 100% control was achieved. Generally, the egg was the most tolerant stage and required 48-, 24-, 16-, and 8-h treatments to achieve 100% mortality at 0.5, 1, 1.5, and 2% NO concentration on dietary media, respectively. Tyrophagus putrescentiae mobile immatures and adult stages were less tolerant, and 100% mortality was achieved after 16-, 8-, 8-, and 4-h treatment at 0.5, 1, 1.5, and 2% NO, respectively. The median lethal concentration (LC50) of NO on egg was 0.86, 0.68, and 0.32% for 8-, 16-, and 24-h treatments. In addition, a confirmatory test was conducted on ham meat at 0.5 and 1.0% of NO and similar efficacy was found. Complete control of egg was achieved after 48- and 24-h treatment at 0.5 and 1.0% of NO, respectively, and larvae and adult mites were 100% controlled after 16 and 8 h at 0.5 and 1.0% of NO, respectively. Our results demonstrated that NO fumigation was effective against T. putrescentiae and can be a potential alternative treatment to methyl bromide for cured-ham pest control.
Topics: Acaridae; Animals; Fumigation; Mites; Nitric Oxide; Pest Control
PubMed: 35148392
DOI: 10.1093/jee/toac014