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Skin Research and Technology : Official... Jun 2024Hypertrophic scars (HS) are a common disfiguring condition in daily clinical encounters which brings a lot of anxieties and concerns to patients, but the treatment...
BACKGROUND
Hypertrophic scars (HS) are a common disfiguring condition in daily clinical encounters which brings a lot of anxieties and concerns to patients, but the treatment options of HS are limited. Black cloth ointment (BCO), as a cosmetic ointment applicable to facial scars, has shown promising therapeutic effects for facial scarring. However, the molecular mechanisms underlying its therapeutic effects remain unclear.
MATERIAL AND METHODS
Network pharmacology was first applied to analyze the major active components of BCO and the related signaling pathways. Subsequently, rabbit ear scar model was successfully established to determine the pharmacological effects of BCO and its active component β-elemene on HS. Finally, the molecular mechanism of BCO and β-elemene was analyzed by Western blot.
RESULTS
Through the network pharmacology, it showed that β-elemene was the main active ingredient of BCO, and it could significantly improve the pathological structure of HS and reduce collagen deposition. BCO and β-elemene could increase the expression of ER stress-related markers and promote the increase of apoptotic proteins in the Western blot experiment and induce the apoptosis of myofibroblasts.
CONCLUSIONS
Our findings indicate that the material basis for the scar-improving effects of the BCO is β-elemene, and cellular apoptosis is the key mechanism through which the BCO and β-elemene exert their effects.
Topics: Cicatrix, Hypertrophic; Rabbits; Animals; Ointments; Network Pharmacology; Disease Models, Animal; Sesquiterpenes; Humans; Apoptosis; Female; Male
PubMed: 38895902
DOI: 10.1111/srt.13791 -
BioRxiv : the Preprint Server For... Jun 2024Protein engineering through the chemical or enzymatic ligation of polypeptide fragments has proven enormously powerful for studying countless biochemical processes . In...
Protein engineering through the chemical or enzymatic ligation of polypeptide fragments has proven enormously powerful for studying countless biochemical processes . In general, this strategy necessitates a protein folding step following ligation of the unstructured fragments, a requirement that constrains the types of systems amenable to the approach. Here, we report an strategy that allows internal regions of target proteins to be replaced in a single operation. Conceptually, our system is analogous to a DNA transposition reaction, but employs orthogonal pairs of split inteins to swap out a designated region of a host protein with an exogenous molecular cassette. We show using isotopic labeling experiments that this 'protein transposition' reaction is concerted when the kinetics for the embedded intein pairs are suitably matched. Critically, this feature allows for efficient manipulation of protein primary structure in the context of a native fold. The utility of this method is illustrated using several protein systems including the multisubunit chromatin remodeling complex, ACF, where we also show protein transposition can occur within the cell nucleus. By carrying out a molecular 'cut and paste' on a protein or protein complex under native folding conditions, our approach dramatically expands the scope of protein semisynthesis.
PubMed: 38895383
DOI: 10.1101/2024.06.03.597171 -
Materials (Basel, Switzerland) Jun 2024This paper presents a method for designing low carbon bio-based building materials, also named bio-concretes, produced with wood wastes in shavings form (WS) and...
This paper presents a method for designing low carbon bio-based building materials, also named bio-concretes, produced with wood wastes in shavings form (WS) and cementitious pastes. As the aggregates phase of bio-concretes is composed of plant-based particles, known as porous and high water-absorbing materials, the bio-concretes cannot be designed by using the traditional design rules used for conventional mortar or concrete. Then, the method used in the current paper is an adaptation of a previous one that has been developed in a recent paper where bio-concretes were produced with a cement matrix, three types of bio-aggregates, and a proposal of a design abacus. However, when that abacus is used for designing WBC with low cement content in the matrix, the target compressive strength is not reached. In the present paper, the method is extended to low cement content matrix (up to 70% of cement substitution) and also considering the greenhouse gas (GHG) emission of the WBC. To obtain data for proposing a new design abacus, an experimental program was carried out by producing nine workable WBCs, varying wood volumetric fractions (40-45-50%), and water-to-binder ratios. The bio-concretes produced presented adequate consistency, lightness (density between 715 and 1207 kg/m), and compressive strength ranging from 0.64 to 12.27 MPa. In addition, the GHG emissions of the WBC were analysed through the Life Cycle Assessment methodology. From the relationships obtained between density, compressive strength, water-to-binder ratio, cement consumption, and GHG emissions of the WBC, calibration constants were proposed for developing the updated and more complete abacus regarding an integrated mix design methodology.
PubMed: 38894006
DOI: 10.3390/ma17112742 -
Materials (Basel, Switzerland) Jun 2024A low-shrinkage and viscosity-reducing polycarboxylate superplasticizer was synthesized with maleic anhydride (MAH), diethylene glycol monobutyl ether, and methoxypoly...
A low-shrinkage and viscosity-reducing polycarboxylate superplasticizer was synthesized with maleic anhydride (MAH), diethylene glycol monobutyl ether, and methoxypoly (ethylene glycol) methacrylate (MPEGMA). The surface tension, early shrinkage, cement paste performance, and application performance of concrete made with the synthesized water-reducing admixture were tested. A series of experiments were conducted to determine the optimal range of plastic viscosity coefficients for producing high-quality, fair-faced concrete with minimal surface defects. These experiments utilized both the synthesized water-reducing admixture alone and in combination with other water-reducing agents. The results showed that the synthesized water-reducing admixture had an ideal molecular structure, as confirmed by the GPC spectrum. When added to an aqueous solution, it reduced the surface tension from 72.47 mN/m to 30.56 mN/m. The 72 h shrinkage value of concrete was reduced by 20.6% compared with that of the conventional control group, effectively reducing shrinkage and adjusting the viscosity of the concrete mixture. Additionally, the influence of the plastic viscosity coefficient on the apparent voids in fair-faced concrete was investigated. This study revealed that when the plastic viscosity coefficient was between 5 and 10 Pa·s, the apparent void grade of the fair-faced concrete was simultaneously excellent and good. This water-reducing admixture helped prevent surface cracking and voids in fair-faced concrete, making it a suitable choice for producing high-quality fair-faced concrete surfaces.
PubMed: 38893950
DOI: 10.3390/ma17112685 -
Materials (Basel, Switzerland) May 2024In this research, the properties and cementitious performance of thermally activated cement pastes (referred to as DCPs) are investigated. Hydrated pastes prepared from...
In this research, the properties and cementitious performance of thermally activated cement pastes (referred to as DCPs) are investigated. Hydrated pastes prepared from Portland cement and slag blended cement were subjected to different thermal treatments: 350 °C for 2 h, 550 °C for 2 h, 550 °C for 24 h and 750 °C for 2 h. The properties and the reactivity as SCM of the DCPs were characterised as well as their effect on the mechanical performance and hydration of new blended cements incorporating the DCPs as supplementary cementitious materials (SCMs). It was observed that the temperature and duration of the thermal treatment increased the grindability and BET specific surface area of the DCP, as well as the formation of CS phases and the reactivity as SCM. In contrast, the mechanical strength results for the blended cements indicated that thermal treatment at 350 °C for 2 h provided better performance. The hydration study results showed that highly reactive DCP interfered with the early hydration of the main clinker phases in Portland cement, leading to early setting and slow strength gain. The effect on blended cement hydration was most marked for binary Portland cement-DCP blends. In contrast, in the case of ternary slag cement-DCP blends the use of reactive DCP as SCM enabled to significantly increase early age strength.
PubMed: 38893922
DOI: 10.3390/ma17112659 -
Materials (Basel, Switzerland) May 2024This study investigated the influences of ultrafine fly ash (UFA) and ultrafine slag powder (USL) on the compressive strengths, autogenous shrinkage, phase assemblage,...
This study investigated the influences of ultrafine fly ash (UFA) and ultrafine slag powder (USL) on the compressive strengths, autogenous shrinkage, phase assemblage, and microstructure of magnesium potassium phosphate cement (MKPC). The findings indicate that the aluminosilicate fractions present in both ultrafine fly ash and ultrafine slag participate in the acid-base reaction of the MKPC system, resulting in a preferential formation of irregularly crystalline struvite-K incorporating Al and Si elements or amorphous aluminosilicate phosphate products. UFA addition mitigates early age autogenous shrinkage in MKPC-based materials, whereas USL exacerbates this shrinkage. In terms of the sustained mechanical strength development of the MKPC system, ultrafine fly ash is preferred over ultrafine slag powder. MKPC pastes with ultrafine fly ash show greater compressive strength compared to those with ultrafine slag powder at 180 days due to denser interfaces between the ultrafine fly ash particles and hydration products like struvite-K. The incorporation of 30 wt% ultrafine fly ash enhances compressive strengths across all testing ages.
PubMed: 38893820
DOI: 10.3390/ma17112556 -
Materials (Basel, Switzerland) May 2024Slag and fly ash (FA) are mostly used as precursors for the production of alkali-activated materials (AAMs). FA is the waste discharged by power plants, while slag and...
Slag and fly ash (FA) are mostly used as precursors for the production of alkali-activated materials (AAMs). FA is the waste discharged by power plants, while slag and steel slag (SS) both belong to the iron and steel industry. The effects of SS and FA on the strength, microstructure, and volume stability of alkali-activated slag (AAS) materials with different water glass modulus (Ms) values were comparatively investigated. The results show that adding SS or FA decreases the compressive strength of AAS mortar, and the reduction effect of SS is more obvious at high Ms. SS or FA reduce the non-evaporable water content (W) of AAS paste. However, SS increases the long-term W of AAS paste at low Ms. The cumulative pore volume and porosity increase after adding SS or FA, especially after adding FA. The hydration products are mainly reticular C-(A)-S-H gels. Adding SS increases the Ca/Si ratio of C-(A)-S-H gel but decreases the Al/Si ratio. However, by mixing FA, the Ca/Si ratio is reduced and the Al/Si ratio is almost unchanged. The incorporation of SS or FA reduces the drying shrinkage of AAS mortar, especially when SS is added. Increasing Ms increases the compressive strength and improves the pore structure, and it significantly increases the drying shrinkage of all samples. This study provides theoretical guidance for the application of steel slag in the alkali-activated slag material.
PubMed: 38893759
DOI: 10.3390/ma17112495 -
Molecules (Basel, Switzerland) Jun 2024Recent interest in the use of waxy starches in food production is due to the possibility of replacing chemically modified starches as texture-forming agents with native...
Recent interest in the use of waxy starches in food production is due to the possibility of replacing chemically modified starches as texture-forming agents with native starch analogues. However, there is a lack of a coherent research comparing different varieties of commercially available waxy starches with respect to their molecular and functional properties. Therefore, the objective of this study was to compare native waxy starches from potatoes, corn, and rice, with particular attention to rheological characteristics in relation to molecular structure. The investigated potato, corn, and rice starch preparations were characterized by significantly different molecular properties due to both botanical origin of starch and variety. The molecular weights of waxy starches were significantly higher than those of their normal counterparts. This phenomenon was accompanied by a more loose conformation of the waxy starch macromolecule in solution. The presence of amylose confers the ability to coagulate starch sol into gel, resulting in substantial changes in the rheological properties of starch paste, and waxy starch pastes being characterized by more viscous flow and smoother texture. Hierarchical cluster analysis indicated that differences between functional properties are more notable for normal than for waxy preparations, in which potato starch, regardless of its variety, was characterized by the most unique characteristics.
PubMed: 38893543
DOI: 10.3390/molecules29112669 -
Molecules (Basel, Switzerland) Jun 2024Nitrite is one of the most common nitrogenous compounds, which is not only an important indicator of aquaculture water but also widely used as a food additive. Its...
Nitrite is one of the most common nitrogenous compounds, which is not only an important indicator of aquaculture water but also widely used as a food additive. Its potential toxicity poses a huge threat to aquatic products and human health. Therefore, it is important to develop a convenient and rapid sensor for the high-efficient onsite detection of nitrite. In this work, a novel electrochemical sensor was developed for the qualitative and quantitative analysis of nitrite. The developed nitrite electrochemical detection system is easily applied in onsite detection. The electrochemical working electrode was constructed based on the combination of Ag-CeO and conductive carbon paste (CPE) with excellent electrocatalysis activity and rapid electron transfer ability. By the application of the developed system and under the optimal conditions, the linear range was from 40.0 μM to 500.0 μM, and the detection limit was reduced to 4.3 μM. The recovery was between 92.1% and 108.1%, and the relative standard deviations (RSDs) were 0.49%~9.31%. The sensor exhibited superior reproducibility, high stability sensitivity, and anti-interference ability, confirming its effectiveness for nitrite analysis. Finally, the developed electrochemical sensor was successfully applied to detect nitrite in beverages and aquaculture water samples, indicating that this approach has great potential in onsite food testing and environmental monitoring.
Topics: Nitrites; Electrochemical Techniques; Cerium; Aquaculture; Beverages; Silver; Limit of Detection; Water Pollutants, Chemical; Electrodes; Reproducibility of Results; Water
PubMed: 38893519
DOI: 10.3390/molecules29112644 -
Molecules (Basel, Switzerland) Jun 2024Due to the high content of impurities such as proteins in tamarind seed polysaccharide (TSP), they must be separated and purified before it can be used. TSP can disperse...
Due to the high content of impurities such as proteins in tamarind seed polysaccharide (TSP), they must be separated and purified before it can be used. TSP can disperse in cold water, but a solution can only be obtained by heating the mixture. Therefore, it is important to understand the dispersion and dissolution process of TSP at different temperatures to expand the application of TSP. In this study, pasting behavior and rheological properties as a function of temperature were characterized in comparison with potato starch (PS), and their relationship with TSP molecular features and microstructure was revealed. Pasting behavior showed that TSP had higher peak viscosity and stronger thermal stability than PS. Rheological properties exhibited that ' and '' of TSP gradually increased with the increase in temperature, without exhibiting typical starch gelatinization behavior. The crystalline or amorphous structure of TSP and starch was disrupted under different temperature treatment conditions. The SEM results show that TSP particles directly transformed into fragments with the temperature increase, while PS granules first expanded and then broken down into fragments. Therefore, TSP and PS underwent different dispersion mechanisms during the dissolution process: As the temperature gradually increased, TSP possibly underwent a straightforward dispersion and was then dissolved in aqueous solution, while PS granules initially expanded, followed by disintegration and dispersion.
Topics: Tamarindus; Polysaccharides; Seeds; Temperature; Rheology; Viscosity; Starch; Chemical Phenomena
PubMed: 38893498
DOI: 10.3390/molecules29112622