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Journal of Economic Entomology Jun 2024Regional variation in pheromone production and response has practical implications for the use of semiochemical lures to monitor and control bark beetle populations. We...
Regional variation in pheromone production and response has practical implications for the use of semiochemical lures to monitor and control bark beetle populations. We tested 4 lure formulations including 2 new formulations that reflect the pheromone production profiles of western and eastern populations of spruce beetles, Dendroctonus rufipennis Kirby (Coleoptera: Curculionidae), as well as 2 commercially available formulations (current Rocky Mountain lure and current Atlantic lure), in 2 locations in New Brunswick, Canada. In 2 separate years, the new eastern lure containing seudenol, MCOL, and spruce terpenes captured 4 times (2021) and 11 times (2022) more spruce beetles than the current Atlantic lure that consisted of frontalin, seudenol, and spruce terpenes. In 2021, we also captured more eastern larch beetles, Dendroctonus simplex LeConte (Coleoptera: Curculionidae), with the new eastern lure, whereas in 2022, we captured the most D. simplex with the current Atlantic lure, suggesting that more research is needed on D. simplex pheromone production and response across its range. The bark beetle predator, Thanasimus dubius (Fabr.; Coleoptera: Cleridae), did not respond well to the new eastern blend that lacks frontalin, suggesting that response to frontalin is important in finding prey and might be conserved in predator populations. The reduced trap catch of T. dubius to the enhanced lure is beneficial because it does not inhibit natural population control by removing predators from the community. Our study reveals an improved trap lure for eastern populations of spruce beetles and highlights gaps and research needs in bark beetle pheromone ecology.
PubMed: 38824447
DOI: 10.1093/jee/toae125 -
The Science of the Total Environment Aug 2024In a mixed forest, certain plants can release allelochemicals that exert allelopathic effects on neighboring plants, thereby facilitating interspecific coexistence of...
In a mixed forest, certain plants can release allelochemicals that exert allelopathic effects on neighboring plants, thereby facilitating interspecific coexistence of two species. Previous studies have demonstrated that allelochemicals released from Ficus carica Linn. roots in mixed forest of F. carica and Taxus cuspidata Sieb. et Zucc. has phase characteristics over time, which can improve the soil physicochemical properties, enzyme activity and microbial diversity, thus promoting the growth of T. cuspidata. Based on the irrigation of exogenous allelochemicals, changes in soil fertility (soil physical and chemical properties, soil enzyme activity and soil microelement content) were observed in response to variations in allelochemicals during five phases of irrigation: initial disturbance phase (0-2 d), physiological compensation phase (2-8 d), screening phase (8-16 d), restore phase (16-32 d) and maturity phase (32-64 d), which was consistent with the response of soil microorganisms. The allelopathic response of growth physiological indexes of T. cuspidata, however, exhibited a slight lag behind the soil fertility, with distinct phase characteristics becoming evident on the 4th day following irrigation of allelochemicals. The findings demonstrated that the allelochemicals released by the root of F. carica induced a synergistic effect on soil fertility and microorganisms, thereby facilitating the growth of T. cuspidata. This study provides a comprehensive elucidation of the phased dynamic response-based allelopathic mechanism employed by F. carica to enhance the growth of T. cuspidata, thus establishing a theoretical basis for optimizing forest cultivation through allelopathic pathways.
Topics: Ficus; Pheromones; Plant Roots; Taxus; Allelopathy; Soil; Soil Microbiology; Plant Exudates
PubMed: 38823714
DOI: 10.1016/j.scitotenv.2024.173663 -
Current Opinion in Insect Science May 2024The common bed bug, Cimex lectularius (Cimicidae, Hemiptera), is obligate haematophagous and can carry pathogens but is not known to transmit diseases to humans in... (Review)
Review
The common bed bug, Cimex lectularius (Cimicidae, Hemiptera), is obligate haematophagous and can carry pathogens but is not known to transmit diseases to humans in natural settings. Bed bugs are activated and guided by semiochemicals and heat by potential hosts, as well as by an aggregation pheromone when searching for food and home. No sexual pheromone has been detected in bed bugs, which mate through traumatic insemination. First, after mounting, males are able to distinguish between large nymphs, other males, and females. To avoid unwanted traumatic inseminations, nymphs and adults emit an alarm pheromone. The olfactory system of bed bugs has relatively few odorant and ionotropic receptors, which likely reflects the simple environment that bed bugs live in, and use to search for hosts and conspecifics.
PubMed: 38821142
DOI: 10.1016/j.cois.2024.101207 -
Translational Psychiatry May 2024Testosterone has complex effects on psychological traits and behavior; it is associated with social dominance and competition and is a potential human sex pheromone....
Testosterone has complex effects on psychological traits and behavior; it is associated with social dominance and competition and is a potential human sex pheromone. This study aimed to investigate the associations between testosterone levels, aggressive behavior, and manic symptoms using a network analysis among bipolar disorder (BD) patients in psychiatric emergency departments (PED). Data from January 2021 and March 2022 BD patients in PED were analyzed. Manic symptoms were assessed using the Young Mania Rating Scale (YMRS). Aggression was assessed with subscale of the PANSS scale (PANSS-AG). The undirected network structures of testosterone levels, aggressive behavior, and manic symptoms were estimated, and centrality and bridge centrality indices were examined. Network stability was examined using the case-dropping procedure. The Network Comparison Test (NCT) was conducted to evaluate whether network characteristics differed by gender. We recruited a total of 898 BD patients, with the mean YMRS score as 13.30 ± 9.58. The prevalence of level II aggression was 35.6% (95%CI = 32.5%-38.7%), level III aggression was 29.5% (95%CI = 26.3%-32.6%), and level VI aggression was 7.0% (95%CI = 5.4%-8.8%). The male participants had a mean testosterone level of 391.71 (Standard Deviation (SD):223.39) compared to 36.90 (SD:30.50) for female participants in the whole sample. Through network analysis, "Increased motor activity-energy" emerged as the central symptom, with the highest centrality expected influence, followed by "Emotional Instability" and "Disruptive/aggression behavior". Notably, "Emotional Instability" appeared to be the bridge symptom linking manic symptoms to aggressive behavior. Within the flow network model, "Speech rate and amount" exhibited the strongest positive correlation with testosterone levels, followed closely by "Disruptive/aggression behavior". The constructed network model demonstrated robust stability, with gender showing no significant impact on the structure. In this study, "Increased motor activity-energy" stood out as the most influential symptom, and "Speech rate and amount" acted as the main bridge symptom linking testosterone levels, aggressive behavior, and manic symptoms. Targeting the central and bridge symptoms may improve the outcomes of aggression interventions implemented among BD patients in psychiatric emergency care.
Topics: Humans; Bipolar Disorder; Aggression; Testosterone; Male; Female; Adult; Cross-Sectional Studies; Middle Aged; Comorbidity; Mania; Psychiatric Status Rating Scales; Young Adult
PubMed: 38811572
DOI: 10.1038/s41398-024-02957-1 -
Veterinary Parasitology Jul 2024The growing challenge of acaricide resistance and geographical range expansion of invasive tick species demands other interventions, like plant-based alternatives, for...
The growing challenge of acaricide resistance and geographical range expansion of invasive tick species demands other interventions, like plant-based alternatives, for sustainable tick control. Leaves, flowers, seedpods, and twig branch extracts of Senna didymobotrya were analyzed using coupled gas chromatography mass spectrometry (GC-MS). Response of adult Amblyomma variegatum and Rhipicephalus appendiculatus to extracts was evaluated. The most attractive plant extract was fractionated and ticks' responses to its fractions assessed. Potential tick attractants in the attractive plant part extract and its fractions were identified by GC-MS analysis. Non- significant qualitative and quantitative differences were observed in the plant parts' extract composition (R = 0.6178). Flower extracts attracted both species, with a 0.1-fold higher attraction in A. variegatum compared to the standard attraction aggregation attachment pheromone (AAAP). Leaf and seedpod extracts repelled ticks at various concentrations. Bioassays after fractionating flower extracts identified hexane and ethyl acetate fractions as most attractive to A. variegatum (P < 0.001) and R. appendiculatus (P < 0.001), respectively. Chemical analysis of the most attractive extracts and fractions identified compounds, including documented acarine attractants, squalene and linoleic acid. A squalene and linoleic acid blend (1:1) at 1 mg/mL significantly attracted adult A. variegatum (P < 0.01) and R. appendiculatus (P < 0.001). The results of this study broaden comprehension of how ticks respond to plants in nature, and showcase the promising potential for integrating these insights into effective tick management programs.
Topics: Animals; Plant Extracts; Rhipicephalus; Amblyomma; Senna Plant; Acaricides; Female; Flowers; Gas Chromatography-Mass Spectrometry; Plant Leaves; Tick Control
PubMed: 38810593
DOI: 10.1016/j.vetpar.2024.110210 -
Molecular Biology of the Cell Jul 2024Spatial and temporal tracking of fluorescent proteins (FPs) in live cells permits visualization of proteome remodeling in response to extracellular cues. Historically,...
Spatial and temporal tracking of fluorescent proteins (FPs) in live cells permits visualization of proteome remodeling in response to extracellular cues. Historically, protein dynamics during trafficking have been visualized using constitutively active FPs fused to proteins of interest. While powerful, such FPs label all cellular pools of a protein, potentially masking the dynamics of select subpopulations. To help study protein subpopulations, bioconjugate tags, including the fluorogen activation proteins (FAPs), were developed. FAPs are comprised of two components: a single-chain antibody (SCA) fused to the protein of interest and a malachite-green (MG) derivative, which fluoresces only when bound to the SCA. Importantly, the MG derivatives can be either cell-permeant or -impermeant, thus permitting isolated detection of SCA-tagged proteins at the cell surface and facilitating quantitative endocytic measures. To expand FAP use in yeast, we optimized the SCA for yeast expression, created FAP-tagging plasmids, and generated FAP-tagged organelle markers. To demonstrate FAP efficacy, we coupled the SCA to the yeast G-protein coupled receptor Ste3. We measured Ste3 endocytic dynamics in response to pheromone and characterized - and -acting regulators of Ste3. Our work significantly expands FAP technology for varied applications in .
Topics: Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins; Protein Transport; Single-Chain Antibodies; Endocytosis; Fluorescent Dyes; Luminescent Proteins; Green Fluorescent Proteins; Rosaniline Dyes
PubMed: 38809589
DOI: 10.1091/mbc.E24-04-0174 -
Scientific Reports May 2024Tribolium castaneum and Rhyzopertha dominica are cosmopolitan, destructive postharvest pests. Although research has investigated how high densities of T. castaneum...
Tribolium castaneum and Rhyzopertha dominica are cosmopolitan, destructive postharvest pests. Although research has investigated how high densities of T. castaneum affect attraction to the aggregation pheromone by conspecifics, research into the behavioral response of both species to food cues after high density exposure has been lacking despite its importance to foraging ecology. Our goal was to manipulate and observe the effects of crowding on the behavioral response of both species to common food and pheromonal stimuli and to determine how the headspace emission patterns from grain differed under increasing densities. Densities of colonies for both species was altered (10-500 adults) on a fixed quantity of food (10 g of flour or whole wheat), then the behavioral response to common food and pheromonal cues was evaluated in a wind tunnel and release-recapture experiment, while volatiles were examined through gas chromatography coupled with mass spectrometry. Importantly, at least for T. castaneum, crowded conditions attenuate attraction to food-based stimuli, but not pheromonal stimuli. Crowding seemed to have no effect on R. dominica attraction to food and pheromonal stimuli in the wind tunnel, but exposure to high density cues did elicit 2.1-3.8-fold higher captures in traps. The relative composition and abundance of headspace volatiles emitted varied significantly with different densities of beetles and was also species-specific. Overall, our results have implications for expanding our understanding of the foraging ecology of two economically important pests.
Topics: Animals; Tribolium; Coleoptera; Feeding Behavior; Pheromones; Population Density; Behavior, Animal
PubMed: 38806558
DOI: 10.1038/s41598-024-62277-8 -
Open Biology May 2024Acorn barnacles are efficient colonizers on a wide variety of marine surfaces. As they proliferate on critical infrastructure, their settlement and growth have... (Comparative Study)
Comparative Study
Acorn barnacles are efficient colonizers on a wide variety of marine surfaces. As they proliferate on critical infrastructure, their settlement and growth have deleterious effects on performance. To address acorn barnacle biofouling, research has focused on the settlement and adhesion processes with the goal of informing the development of novel coatings. This effort has resulted in the discovery and characterization of several proteins found at the adhesive substrate interface, i.e. cement proteins, and a deepened understanding of the function and composition of the biomaterials within this region. While the adhesive properties at the interface are affected by the interaction between the proteins, substrate and mechanics of the calcified base plate, little attention has been given to the interaction between the proteins and the cuticular material present at the substrate interface. Here, the proteome of the organic matrix isolated from the base plate of the acorn barnacle is compared with the chitinous and proteinaceous matrix embedded within parietal plates. The objective was to gain an understanding of how the basal organic matrix may be specialized for adhesion via an in-depth comparative proteome analysis. In general, the majority of proteins identified in the parietal matrix were also found in the basal organic matrix, including nearly all those grouped in classes of cement proteins, enzymes and pheromones. However, the parietal organic matrix was enriched with cuticle-associated proteins, of which 30% of those identified were unique to the parietal region. In contrast, 30-40% of the protease inhibitors, enzymes and pheromones identified in the basal organic matrix were unique to this region. Not unexpectedly, nearly 50% of the cement proteins identified in the basal region were significantly distinct from those found in the parietal region. The wider variety of identified proteins in the basal organic matrix indicates a greater diversity of biological function in the vicinity of the substrate interface where several processes related to adhesion, cuticle formation and expansion of the base synchronize to play a key role in organism survival.
Topics: Animals; Thoracica; Proteomics; Proteome; Biofouling; Arthropod Proteins
PubMed: 38806147
DOI: 10.1098/rsob.230246 -
Environmental Entomology May 2024
PubMed: 38805700
DOI: 10.1093/ee/nvae055 -
BioRxiv : the Preprint Server For... May 2024respond to mating pheromone through the GPCRs Ste2 and Ste3, which promote growth of a mating projection in response to ligand binding. This commitment to mating is...
UNLABELLED
respond to mating pheromone through the GPCRs Ste2 and Ste3, which promote growth of a mating projection in response to ligand binding. This commitment to mating is nutritionally and energetically taxing, and so we hypothesized that the cell may suppress mating signaling during starvation. We set out to investigate negative regulators of the mating pathway in nutritionally depleted environments. Here, we report that nutrient deprivation led to loss of Ste2 from the plasma membrane. Recapitulating this effect with nitrogen starvation led us to hypothesize that it was due to TORC1 signaling. Rapamycin inhibition of TORC1 impacted membrane levels of all yeast GPCRs. Inhibition of TORC1 also dampened mating pathway output. Deletion analysis revealed that TORC1 repression leads to α-arrestin-directed CME through TORC2-Ypk1 signaling. We then set out to determine whether major downstream effectors of the TOR complexes also downregulate pathway output during mating. We found that autophagy contributes to pathway downregulation through analysis of strains lacking . We also show that Ypk1 significantly reduced pathway output. Thus, both autophagy machinery and TORC2-Ypk1 signaling serve as attenuators of pheromone signaling during mating. Altogether, we demonstrate that the stress-responsive TOR complexes coordinate GPCR endocytosis and reduce the magnitude of pheromone signaling, in ligand-independent and ligand-dependent contexts.
ONE SENTENCE SUMMARY
TOR signaling regulates the localization of all GPCRs during starvation and suppress the mating pathway in the presence and absence of ligand.
PubMed: 38798445
DOI: 10.1101/2024.05.09.593412