-
International Journal of Pharmaceutics Jun 2024Breast cancer has now replaced lung cancer as the most prevalent malignant tumor worldwide, posing a serious health risk to women. We have recently designed a promising...
Breast cancer has now replaced lung cancer as the most prevalent malignant tumor worldwide, posing a serious health risk to women. We have recently designed a promising option strategy for the treatment of breast cancer. In this work, cyclodextrin metal-organic frameworks with high drug-carrying properties were endo-crosslinked by 3,3'dithiodipropionyl chloride to form cubic phase gel nanoparticles, which were drug-loaded and then coated by MCF-7 cell membranes. After intravenous injection, this multifunctional nanomedicine achieved dramatically homologous targeting co-delivery of honokiol and indocyanine green to the breast tumor. Further, the disulfide bonds in the nanostructures achieved glutathione-responsive drug release, induced tumor cells to produce reactive oxygen species and promoted apoptosis, resulting in tumor necrosis, and at the same time, inhibited Ki67 protein expression, which enhanced photochemotherapy, and resulted in a 94.08 % in vivo tumor suppression rate in transplanted tumor-bearing mice. Thereby, this nanomimetic co-delivery system may have a place in breast cancer therapy due to its simple fabrication process, excellent biocompatibility, efficient targeted delivery of insoluble drugs, and enhanced photochemotherapy.
PubMed: 38848796
DOI: 10.1016/j.ijpharm.2024.124310 -
Journal of Refractive Surgery... May 2024To compare the effects of corneal allogenic intrastromal ring segment (CAIRS) implantation on topographical measurements and visual outcomes of patients with keratoconus... (Comparative Study)
Comparative Study
PURPOSE
To compare the effects of corneal allogenic intrastromal ring segment (CAIRS) implantation on topographical measurements and visual outcomes of patients with keratoconus with and without corneal cross-linking (CXL) prior to the time of implantation.
METHODS
Sixty-seven eyes with corneal allograft intrastromal ring segment implantation (KeraNatural; Lions VisionGift) due to advanced keratoconus were included in the study. Thirty-seven eyes had no CXL and 30 eyes had had CXL before being referred to the authors. The changes in spherical equivalent (SE), uncorrected distance visual acuity (UDVA), corrected distance visual acuity (CDVA), steep keratometry (K1), flat keratometry (K2), mean keratometry (Kmean), maximum keratometry (Kmax), and thinnest pachymetry were retrospectively analyzed 6 months after the implantation.
RESULTS
The median age was 29 years in the CXL group and 24.0 years in the non-CXL group ( > .05), respectively. All topographical and visual parameters before implantation were similar in both groups ( > .05 for all parameters). At 6 months, CDVA, K1, and Kmean showed higher improvement in the non-CXL group than the CXL group ( = .030, .018, and .039, respectively).
CONCLUSIONS
CAIRS surgery has a flattening effect on both the corneas with and without CXL. The cornea with prior CXL treatment had less flattening effect due to the stiffening effect of prior CXL. .
Topics: Humans; Keratoconus; Corneal Stroma; Cross-Linking Reagents; Visual Acuity; Adult; Male; Female; Prosthesis Implantation; Photosensitizing Agents; Retrospective Studies; Corneal Topography; Young Adult; Prostheses and Implants; Refraction, Ocular; Collagen; Corneal Pachymetry; Riboflavin; Photochemotherapy; Adolescent; Ultraviolet Rays; Corneal Transplantation; Middle Aged; Corneal Cross-Linking
PubMed: 38848056
DOI: 10.3928/1081597X-20240501-01 -
ACS Applied Materials & Interfaces Jun 2024Multidrug-resistant (MDR) bacteria pose serious threats to public health due to the lack of effective and biocompatible drugs to kill MDR bacteria. Photodynamic...
Multidrug-resistant (MDR) bacteria pose serious threats to public health due to the lack of effective and biocompatible drugs to kill MDR bacteria. Photodynamic antibacterial therapy has been widely studied due to its low induction of resistance. However, photosensitizers that can efficiently generate reactive oxygen species (ROS) through both type I and type II mechanisms and that have the capability of multiple modes of action are rarely reported. Addressing this issue, we developed a near-infrared-emitting triphenylamine indole iodoethane (TTII) and its silver(I) self-assembled (TTIIS) aggregation-induced emission (AIE) photosensitizer for multimode bacterial infection therapy. TTII can efficiently produce both Type I ROS OH and Type II ROS O. Interestingly, the Ag(I)-π interaction contributed in TTIIS efficiency promotion of the generation of O. Moreover, by releasing Ag, TTIIS enabled photodynamic-Ag(I) dual-mode sterilization. As a result, TTIIS achieved an effective enhancement of antibacterial activity, with a 1-2-fold boost against multidrug-resistant (MDR ). Both TTII and TTIIS at a concentration as low as 0.55 μg mL can kill more than 98% of methicillin resistant (MRSA) on MRSA-infected full-thickness defect wounds of a mouse, and both TTII and TTIIS were effective in eliminating the bacteria and promoting wound healing.
Topics: Photosensitizing Agents; Silver; Animals; Drug Resistance, Multiple, Bacterial; Mice; Anti-Bacterial Agents; Escherichia coli; Reactive Oxygen Species; Photochemotherapy; Microbial Sensitivity Tests; Methicillin-Resistant Staphylococcus aureus
PubMed: 38847621
DOI: 10.1021/acsami.4c05202 -
ACS Applied Materials & Interfaces Jun 2024The prevalence of pathogenic bacterial infections with high morbidity and mortality poses a widespread challenge to the healthcare system. Therefore, it is imperative to...
Dual pH- and ATP-Responsive Antibacterial Nanospray: On-Demand Release of Antibacterial Factors, Imaging Monitoring, and Accelerated Healing of Bacteria-Infected Wounds under NIR Activation.
The prevalence of pathogenic bacterial infections with high morbidity and mortality poses a widespread challenge to the healthcare system. Therefore, it is imperative to develop nanoformulations capable of adaptively releasing antimicrobial factors and demonstrating multimodal synergistic antimicrobial activity. Herein, an NIR-activated multifunctional synergistic antimicrobial nanospray MXene/ZIF-90@ICG was prepared by incorporating ZIF-90@ICG nanoparticles onto MXene-NH nanosheets. MXene/ZIF-90@ICG can on-demand release the antimicrobial factors MXenes, ICG, and Zn in response to variations in pH and ATP levels within the bacterial infection microenvironment. Under NIR radiation, the combination of MXenes, Zn, and ICG generated a significant amount of ROS and elevated heat, thereby enhancing the antimicrobial efficacy of PDT and PTT. Meanwhile, NIR excitation could accelerate the further release of ICG and Zn, realizing the multimodal synergistic antibacterial effect of PDT/PTT/Zn. Notably, introducing MXenes improved the dispersion of the synthesized antimicrobial nanoparticles in aqueous solution, rendering MXene/ZIF-90@ICG a candidate for application as a nanospray. Importantly, MXene/ZIF-90@ICG demonstrated antimicrobial activity and accelerated wound healing in the constructed subcutaneous infection model with NIR activation, maintaining a favorable biosafety level. Therefore, MXene/ZIF-90@ICG holds promise as an innovative nanospray for adaptive multimodal synergistic and efficient antibacterial applications with NIR activation.
Topics: Anti-Bacterial Agents; Animals; Wound Healing; Hydrogen-Ion Concentration; Staphylococcus aureus; Infrared Rays; Adenosine Triphosphate; Mice; Indocyanine Green; Nanoparticles; Microbial Sensitivity Tests; Metal-Organic Frameworks; Escherichia coli; Humans; Photochemotherapy
PubMed: 38847598
DOI: 10.1021/acsami.4c03587 -
Photodermatology, Photoimmunology &... Jul 2024Infections are complications in the wound healing process, and their treatment can lead to antibiotic overuse and bacterial resistance. Antimicrobial photodynamic...
BACKGROUND
Infections are complications in the wound healing process, and their treatment can lead to antibiotic overuse and bacterial resistance. Antimicrobial photodynamic therapy (aPDT) is used to treat infectious diseases caused by fungi, viruses, or bacteria. Methylene blue (MB) and its derivatives are commonly used dyes in antimicrobial photodynamic therapy (aPDT-MB).
METHODS
This study is a PRISMA systematic review of animal models used to discuss the usefulness and therapeutic parameters of aPDT-MB or its derivatives for treating infected skin wounds.
RESULTS
After an extensive literature review, 13 controlled trials totaling 261 animals were selected to evaluate skin infection by leishmaniasis and cutaneous bacterial and fungal infections. All studies found results favoring the use of aPDT-MB. Great variability in parameters was found for radiant exposure from 12 to 360 J/cm, MB diluted in saline solution or distilled water, irradiation time from 40 to 3600 s, irradiance most commonly at a maximum of 100 mW/cm, and wavelength used mainly in the 630-670 nm range.
CONCLUSION
MB is a safe and promising agent used as a photosensitizer in aPDT for skin-infected lesions. There is great variability in the parameters found. Comparisons concerning concentration, irradiation time, and light intensity need to be performed.
Topics: Animals; Disease Models, Animal; Methylene Blue; Photochemotherapy; Photosensitizing Agents
PubMed: 38845020
DOI: 10.1111/phpp.12978 -
Planta Medica Jun 2024Antimicrobial photodynamic therapy (aPDT) is an evolving treatment strategy against human pathogenic microbes such as the species, including the emerging pathogen ....
Antimicrobial photodynamic therapy (aPDT) is an evolving treatment strategy against human pathogenic microbes such as the species, including the emerging pathogen . Using a modified EUCAST protocol, the light-enhanced antifungal activity of the natural compound parietin was explored. The photoactivity was evaluated against three separate strains of five yeasts, and its molecular mode of action was analysed via several techniques, i.e., cellular uptake, reactive electrophilic species (RES), and singlet oxygen yield. Under experimental conditions ( = 428 nm, H = 30 J/cm, PI = 30 min), microbial growth was inhibited by more than 90% at parietin concentrations as low as c = 0.156 mg/L (0.55 µM) for and , c = 0.313 mg/L (1.10 µM) for , c = 0.625 mg/L (2.20 µM) for , and c = 1.250 mg/L (4.40 µM) for . Mode-of-action analysis demonstrated fungicidal activity. Parietin targets the cell membrane and induces cell death via ROS-mediated lipid peroxidation after light irradiation. In summary, parietin exhibits light-enhanced fungicidal activity against all species tested (including ) and , covering three of the four critical threats on the WHO's most recent fungal priority list.
Topics: Antifungal Agents; Cryptococcus neoformans; Microbial Sensitivity Tests; Candida auris; Light; Candida; Reactive Oxygen Species; Photochemotherapy; Anthraquinones; Photosensitizing Agents
PubMed: 38843798
DOI: 10.1055/a-2249-9110 -
ACS Applied Materials & Interfaces Jun 2024Dental caries is a widespread bacterial infectious disease that imposes a significant public health burden globally. The primary culprits in caries development are...
Dental caries is a widespread bacterial infectious disease that imposes a significant public health burden globally. The primary culprits in caries development are cariogenic bacteria, notably (), due to their robust biofilm-forming capabilities. To address this issue, a series of cationic pyridinium-substituted photosensitizers with aggregation-induced emission have been designed. All of these aggregation-induced emission luminogens (AIEgens) exhibit outstanding microbial visualization and photodynamic killing of , thanks to their luminous fluorescence and efficient singlet oxygen generation ability. Notably, one of the membrane-anchored AIEgens (TDTPY) can inactivate planktic and its biofilm without causing significant cytotoxicity. Importantly, application of TDTPY-mediated photodynamic treatment on in vivo rodent models has yielded commendable imaging results and effectively slowed down caries progression with assured biosafety. Unlike traditional single-mode anticaries materials, AIEgens integrate the dual functions of detecting and removing and are expected to build a new caries management diagnosis and treatment platform. To the best of our knowledge, this is also the first report on the use of AIEgens for anticaries studies both in vitro and in vivo.
Topics: Streptococcus mutans; Photosensitizing Agents; Dental Caries; Photochemotherapy; Animals; Biofilms; Mice; Singlet Oxygen; Humans; Anti-Bacterial Agents
PubMed: 38842123
DOI: 10.1021/acsami.4c04585 -
Journal of Nanobiotechnology Jun 2024Adoptive cellular immunotherapy as a promising and alternative cancer therapy platform is critical for future clinical applications. Natural killer (NK) cells have...
Adoptive cellular immunotherapy as a promising and alternative cancer therapy platform is critical for future clinical applications. Natural killer (NK) cells have attracted attention as an important type of innate immune regulatory cells that can rapidly kill multiple adjacent cancer cells. However, these cells are significantly less effective in treating solid tumors than in treating hematological tumors. Herein, we report the synthesis of a FeO-PEG-CD56/Avastin@Ce6 nanoprobe labeled with NK-92 cells that can be used for adoptive cellular immunotherapy, photodynamic therapy and dual-modality imaging-based in vivo fate tracking. The labeled NK-92 cells specifically target the tumor cells, which increases the amount of cancer cell apoptosis in vitro. Furthermore, the in vivo results indicate that the labeled NK-92 cells can be used for tumor magnetic resonance imaging and fluorescence imaging, adoptive cellular immunotherapy, and photodynamic therapy after tail vein injection. These data show that the developed multifunctional nanostructure is a promising platform for efficient innate immunotherapy, photodynamic treatment and noninvasive therapeutic evaluation of breast cancer.
Topics: Breast Neoplasms; Humans; Female; Killer Cells, Natural; Animals; Photochemotherapy; Mice; Polyethylene Glycols; Cell Line, Tumor; CD56 Antigen; Immunotherapy, Adoptive; Apoptosis; Magnetic Resonance Imaging; Mice, Inbred BALB C; Mice, Nude
PubMed: 38840120
DOI: 10.1186/s12951-024-02599-x -
Lasers in Medical Science Jun 2024The aim of this study was to compare two types of light irradiation devices for antimicrobial photodynamic therapy (aPDT). A 660-nm light-emitting diode (LED) and a... (Comparative Study)
Comparative Study
The aim of this study was to compare two types of light irradiation devices for antimicrobial photodynamic therapy (aPDT). A 660-nm light-emitting diode (LED) and a 665-nm laser diode (LD) were used for light irradiation, and 0.1 mg/L TONS 504, a cationic chlorin derivative, was used as the photosensitizer. We evaluated the light attenuation along the vertical and horizontal directions, temperature rise following light irradiation, and aPDT efficacy against Staphylococcus aureus under different conditions: TONS 504 only, light irradiation only, and TONS 504 with either LED (30 J/cm) or LD light irradiation (continuous: 30 J/cm; pulsed: 20 J/cm at 2/3 duty cycle, 10 J/cm at 1/3 duty cycle). Both LED and LD light intensities were inversely proportional to the square of the vertical distance from the irradiated area. Along the horizontal distance from the nadir of the light source, the LED light intensity attenuated according to the cosine quadrature law, while the LD light intensity did not attenuate within the measurable range. Following light irradiation, the temperature rise increased as the TONS 504 concentration increased in the order of pulsed LD < continuous LD < LED irradiation. aPDT with light irradiation only or TONS 504 only had no antimicrobial effect, while aPDT with TONS 504 under continuous or pulsed LD light irradiation provided approximately 3 log reduction at 30 J/cm and 20 J/cm and approximately 2 log reduction at 10 J/cm. TONS 504-aPDT under pulsed LD light irradiation provided anti-microbial effect without significant temperature rise.
Topics: Photochemotherapy; Staphylococcus aureus; Photosensitizing Agents; Humans; Lasers, Semiconductor; Porphyrins; Temperature
PubMed: 38839711
DOI: 10.1007/s10103-024-04103-1 -
ACS Applied Materials & Interfaces Jun 2024Currently, photodynamic therapy (PDT) is restricted by the laser penetration depth. Except for PDT at 1064 nm wavelength excitation, the development of other...
Currently, photodynamic therapy (PDT) is restricted by the laser penetration depth. Except for PDT at 1064 nm wavelength excitation, the development of other NIR-II-activated nanomaterials with a higher response depth is still hindered and rarely reported in the literature. To overcome these problems, we fabricated a nanoplatform with heterostructures that generate reactive oxygen species (ROS) and ferrite nanoparticles under a high concentration of zinc doping (ZnFeO NPs), which can achieve oxidative damage of tumor cells under near-infrared (NIR) illumination. The recombination of photoelectrons and holes has been markedly inhibited due to the formation of heterostructures in the interfaces, thus greatly enhancing the capability for ROS and oxygen production by modulating the single-component doping content. The efficiency of PDT was verified by and assays under NIR light. Our results revealed that NIR-II (1208 nm) light irradiation of ZnFeO NPs exerted a remarkable antitumor activity, superior to NIR-I light (808 nm). More importantly, the reported ZnFeO NPs strategy provides an opportunity for the success of comparison with light in the first and second near-infrared regions.
Topics: Photochemotherapy; Humans; Zinc; Infrared Rays; Animals; Mice; Reactive Oxygen Species; Photosensitizing Agents; Ferric Compounds; Antineoplastic Agents; Cell Line, Tumor; Mice, Inbred BALB C
PubMed: 38833169
DOI: 10.1021/acsami.4c05717