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Journal of Materials Chemistry. B Jun 2024In this study, the heavy-atom-free BODIPY dendrimer TM-BDP was synthesized for near-infrared photodynamic therapy, and was composed of a triphenylamine-BODIPY dimer and...
In this study, the heavy-atom-free BODIPY dendrimer TM-BDP was synthesized for near-infrared photodynamic therapy, and was composed of a triphenylamine-BODIPY dimer and four 1-(2-morpholinoethyl)-1-indole-3-ethenyl groups. The TM-BDP could achieve near-infrared photodynamic therapy through two different photosensitive pathways, which include one-photon excitation at 660 nm and two-photon excitation at 1000 nm. In the one-photon excitation pathway, the TM-BDP could generate singlet oxygen and superoxide radicals under 660 nm illumination. In addition, the one-photon PDT experiment in human nasopharyngeal carcinoma (CNE-2) cells also indicated that the TM-BDP could specifically accumulate in lysosomes and show great cell phototoxicity with an IC of 22.1 μM. In the two-photon excitation pathway, the two-photon absorption cross-section at 1030 nm of TM-BDP was determined to be 383 GM, which means that it could generate reactive oxygen species (ROS) under 1000 nm femtosecond laser excitation. Moreover, the two-photon PDT experiment in zebrafish also indicated the TM-BDP could be used for two-photon fluorescence imaging and two-photon induced ROS generation in biological environments. Furthermore, in terms of the ROS generation mechanism, the TM-BDP employed a novel spin-vibronic coupling intersystem crossing (SV-ISC) process for the mechanism of ROS generation and the femtosecond transient absorption spectra indicated that this novel SV-ISC mechanism was closely related to its charge transfer state lifetime. These above experiments of TM-BDP demonstrate that the dendrimer design is an effective strategy for constructing heavy-atom-free BODIPY photosensitizers in the near-infrared region and lay the foundation for two-photon photodynamic therapy in future clinical trials.
Topics: Zebrafish; Animals; Photochemotherapy; Boron Compounds; Dendrimers; Photons; Photosensitizing Agents; Humans; Molecular Structure; Reactive Oxygen Species; Cell Survival; Cell Line, Tumor
PubMed: 38831689
DOI: 10.1039/d4tb00535j -
ACS Macro Letters Jun 2024The low therapeutic efficacy and potential long-term toxicity of antitumor treatments seriously limit the clinical application of phototherapies. Herein, we develop a...
The low therapeutic efficacy and potential long-term toxicity of antitumor treatments seriously limit the clinical application of phototherapies. Herein, we develop a degradable phototheranostic nanoplatform for NIR-II fluorescence bioimaging-guided synergistic photothermal (PTT) and photodynamic therapies (PDT) and immune activation to inhibit tumor growth. The phototheranostic nanoplatform (CX@PSS) consists of multidisulfide-containing polyurethane loaded with a photosensitizer CX, which can be specifically degraded in the GSH overexpressed tumor microenvironment (TME) and exhibits good NIR-II fluorescence, photodynamic, and photothermal properties. Under 808 nm light irradiation, CX@PSS exhibits efficient photothermal conversion and ROS generation, which further induces immunogenic cell death (ICD), releasing tumor-associated antigens and activating the immune response. and studies confirm the potential of CX@PSS in NIR II FL imaging-guided tumor treatments by synergistic PTT, PDT, and immune activation. This work is expected to provide a new pathway for clinical applications of imaging-guided tumor diagnosis and treatments.
Topics: Tumor Microenvironment; Animals; Mice; Humans; Photosensitizing Agents; Theranostic Nanomedicine; Photochemotherapy; Optical Imaging; Infrared Rays; Nanoparticles; Cell Line, Tumor; Neoplasms; Photothermal Therapy; Polyurethanes
PubMed: 38829688
DOI: 10.1021/acsmacrolett.4c00251 -
Journal of Materials Chemistry. B Jun 2024Due to the rapid progression and aggressive metastasis of breast cancer, its diagnosis and treatment remain a great challenge. The simultaneous inhibition of tumor...
Due to the rapid progression and aggressive metastasis of breast cancer, its diagnosis and treatment remain a great challenge. The simultaneous inhibition of tumor growth and metastasis is necessary for breast cancer to obtain ideal therapeutic outcomes. We herein report the development of radioactive hybrid semiconducting polymer nanoparticles (SPN) for imaging-guided tri-modal therapy of breast cancer. Two semiconducting polymers are used to form SPN with a diameter of around 60 nm nano-coprecipitation and they are also labeled with iodine-131 (I) to enhance the imaging functions. The formed SPN show good radiolabeling stability and excellent photodynamic and photothermal effects under 808 nm laser irradiation to produce singlet oxygen (O) and heat. Moreover, SPN can generate O with ultrasound irradiation their sonodynamic properties. After intravenous tail vein injection, SPN can effectively accumulate in the subcutaneous 4T1 tumors of living mice as verified fluorescence and single photon emission computed tomography (SPECT) imaging. With the irradiation of tumors using an 808 nm laser and US, SPN mediate photodynamic therapy (PDT), photothermal therapy (PTT) and sonodynamic therapy (SDT) to kill tumor cells. Such a tri-modal therapy leads to an improved efficacy in inhibiting tumor growth and suppressing tumor metastasis compared to the sole SDT and combinational PDT-PTT. This study thus demonstrates the applications of SPN to diagnose tumors and combine different therapies for effective breast cancer treatment.
Topics: Animals; Nanoparticles; Mice; Female; Polymers; Iodine Radioisotopes; Breast Neoplasms; Semiconductors; Photochemotherapy; Mice, Inbred BALB C; Humans; Cell Proliferation; Antineoplastic Agents; Cell Line, Tumor; Particle Size; Tomography, Emission-Computed, Single-Photon; Photothermal Therapy; Mammary Neoplasms, Experimental
PubMed: 38828732
DOI: 10.1039/d4tb00834k -
Spectrochimica Acta. Part A, Molecular... Oct 2024Considering the increasing number of pathogens resistant to commonly used antibiotics as well as antiseptics, there is an urgent need for antimicrobial approaches that...
Considering the increasing number of pathogens resistant to commonly used antibiotics as well as antiseptics, there is an urgent need for antimicrobial approaches that can effectively inactivate pathogens without the risk of establishing resistance. An alternative approach in this context is antibacterial photodynamic therapy (APDT). APDT is a process that involves bacterial cell death using appropriate wavelength light energy and photosensitizer and causes the production of reactive oxygen species inside or outside the microbial cell depending on the penetration of light energy. In our study, a new porphyrin compound 4,4'-methylenebis(2-((E)-((4-(10,15,20-triphenylporphyrin-5-yl)phenyl)imino)methyl)phenol) (SP) was designed and synthesized as photosensitizer and its structure was clarified by NMR (C and H) and mass determination method. Photophysical and photochemical properties were examined in detail using different methods. Singlet oxygen quantum yields were obtained as 0.48 and 0.59 by direct and indirect methods, respectively. Antibacterial activity studies have been conducted within the scope of biological activity and promising results have been obtained under LED light (500-700 nm, 265 V, 1500 LM), contributing to the antibacterial photodynamic therapy literature.
Topics: Photochemotherapy; Photosensitizing Agents; Porphyrins; Anti-Bacterial Agents; Singlet Oxygen; Microbial Sensitivity Tests; Light; Bacteria; Drug Design
PubMed: 38824758
DOI: 10.1016/j.saa.2024.124529 -
Journal of Colloid and Interface Science Oct 2024Chemotherapy and surgery stand as primary cancer treatments, yet the unique traits of the tumor microenvironment hinder their effectiveness. The natural compound...
Chemotherapy and surgery stand as primary cancer treatments, yet the unique traits of the tumor microenvironment hinder their effectiveness. The natural compound epigallocatechin gallate (EGCG) possesses potent anti-tumor and antibacterial traits. However, the tumor's adaptability to chemotherapy due to its acidic pH and elevated glutathione (GSH) levels, coupled with the challenges posed by drug-resistant bacterial infections post-surgery, impede treatment outcomes. To address these challenges, researchers strive to explore innovative treatment strategies, such as multimodal combination therapy. This study successfully synthesized Cu-EGCG, a metal-polyphenol network, and detailly characterized it by using synchrotron radiation and high-resolution mass spectrometry (HRMS). Through chemodynamic therapy (CDT), photothermal therapy (PTT), and photodynamic therapy (PDT), Cu-EGCG showed robust antitumor and antibacterial effects. Cu in Cu-EGCG actively participates in a Fenton-like reaction, generating hydroxyl radicals (·OH) upon exposure to hydrogen peroxide (HO) and converting to Cu. This Cu interacts with GSH, weakening the oxidative stress response of bacteria and tumor cells. Density functional theory (DFT) calculations verified Cu-EGCG's efficient GSH consumption during its reaction with GSH. Additionally, Cu-EGCG exhibited outstanding photothermal conversion when exposed to 808 nm near-infrared (NIR) radiation and produced singlet oxygen (O) upon laser irradiation. In both mouse tumor and wound models, Cu-EGCG showcased remarkable antitumor and antibacterial properties.
Topics: Anti-Bacterial Agents; Copper; Nanocomposites; Antineoplastic Agents; Animals; Mice; Humans; Catechin; Microbial Sensitivity Tests; Drug Resistance, Bacterial; Photochemotherapy; Wound Infection; Drug Screening Assays, Antitumor; Staphylococcus aureus; Photothermal Therapy; Particle Size; Escherichia coli; Cell Survival; Cell Line, Tumor; Surface Properties; Cell Proliferation
PubMed: 38824748
DOI: 10.1016/j.jcis.2024.05.080 -
Colloids and Surfaces. B, Biointerfaces Aug 2024Photoactivated therapy has gradually emerged as a promising and rapid method for combating bacteria, aimed at overcoming the emergence of drug-resistant strains...
Photoactivated therapy has gradually emerged as a promising and rapid method for combating bacteria, aimed at overcoming the emergence of drug-resistant strains resulting from the inappropriate use of antibiotics and the subsequent health risks. In this work, we report the facile fabrication of Zn[Fe(CN)]/g-CN nanocomposites (denoted as ZHF/g-CN) through the in-situ loading of zinc hexacyanoferrate nanospheres onto two-dimensional g-CN sheets using a simple metal-organic frameworks construction method. The ZHF/g-CN nanocomposite exhibits enhanced antibacterial activity through the synergistic combination of the excellent photothermal properties of ZHF and the photodynamic capabilities of g-CN. Under dual-light irradiation (420 nm + 808 nm NIR), the nanocomposites achieve remarkable bactericidal efficacy, eliminating 99.98% of Escherichia coli and 99.87% of Staphylococcus aureus within 10 minutes. Furthermore, in vivo animal experiments have demonstrated the outstanding capacity of the composite in promoting infected wound healing, achieving a remarkable wound closure rate of 99.22% after a 10-day treatment period. This study emphasizes the potential of the ZHF/g-CN nanocomposite in effective antimicrobial applications, expanding the scope of synergistic photothermal/photodynamic therapy strategies.
Topics: Nanocomposites; Wound Healing; Staphylococcus aureus; Escherichia coli; Anti-Bacterial Agents; Animals; Photochemotherapy; Microbial Sensitivity Tests; Mice; Sterilization; Ferrocyanides; Particle Size; Zinc; Photothermal Therapy; Surface Properties; Nitrogen Compounds; Graphite
PubMed: 38823340
DOI: 10.1016/j.colsurfb.2024.113998 -
Journal of Inorganic Biochemistry Sep 2024A strategy for cancer treatment was implemented, based on chemo-photodynamic therapy, utilizing a novel formulation, low-cost system called Cas-ZnONPs. This system...
A strategy for cancer treatment was implemented, based on chemo-photodynamic therapy, utilizing a novel formulation, low-cost system called Cas-ZnONPs. This system consisted of the incorporation of Casiopeina III-ia (CasIII-ia), a hydrophilic copper coordination compound with well-documented anti-neoplastic activity, on Zinc oxide nanoparticles (ZnONPs) with apoptotic activity and lipophilicity, allowing them to permeate biological barriers. Additionally, ZnONPs exhibited fluorescence, with emission at different wavelengths depending on their agglomeration and enabling real-time tracking biodistribution. Also, ZnONPs served as a sensitizer, generating reactive oxygen species (ROS) in situ. In in vitro studies on HeLa and MDA-MB-231 cell lines, a synergistic effect was observed with the impregnated CasIII-ia on ZnONPs. The anticancer activity had an increase in cellular inhibition, depending on the dose of exposure to UV-vis irradiation. In in vivo studies utilized zebrafish models for xenotransplanting stained MDA-MB-231 cells and testing the effectiveness of Cas-ZnONPs treatment. The treatment successfully eliminated cancer cells, both when combined with Photodynamic Therapy (PDT) and when used alone. However, a significantly higher concentration (50 times) of Cas-ZnONPs was required in the absence of PDT. This demonstrates the potential of Cas-ZnONPs in cancer treatment, especially when combined with PDT.
Topics: Humans; Photochemotherapy; Animals; Zebrafish; Antineoplastic Agents; Zinc Oxide; HeLa Cells; Reactive Oxygen Species; Photosensitizing Agents; Cell Line, Tumor; Nanoparticles; Apoptosis; Coordination Complexes; Copper
PubMed: 38823065
DOI: 10.1016/j.jinorgbio.2024.112623 -
Inorganic Chemistry Jun 2024Two Ru(II) complexes, [Ru(pydppn)(bim)(py)] [; pydppn = 3-(pyrid-2'-yl)-4,5,9,16-tetraaza-dibenzo[]naphthacene; bim = 2,2'-bisimidazole; py = pyridine] and...
Two Ru(II) complexes, [Ru(pydppn)(bim)(py)] [; pydppn = 3-(pyrid-2'-yl)-4,5,9,16-tetraaza-dibenzo[]naphthacene; bim = 2,2'-bisimidazole; py = pyridine] and [Ru(pydppn)(Mebim)(py)] [; Mebim = 2,2'-bis(4,5-dimethylimidazole)], were synthesized and characterized, and their photophysical properties, DNA binding, and photocleavage were evaluated and compared to [Ru(pydppn)(bpy)(py)] (; bpy = 2,2'-bipyridine). Complexes and exhibit broad MLCT (metal-to-ligand charge transfer) transitions with maxima at ∼470 nm and shoulders at ∼525 and ∼600 nm that extend to ∼800 nm. These bands are red-shifted relative to those of , attributed to the π-donating ability of the bim and Mebim ligands. A strong signal at 550 nm is observed in the transient absorption spectra of -, previously assigned as arising from a pydppn-centered ππ* state, with lifetimes of ∼19 μs for and and ∼270 ns for . A number of methods were used to characterize the mode of binding of - to DNA, including absorption titrations, thermal denaturation, relative viscosity changes, and circular dichroism, all of which point to the intercalation of the pydpppn ligand between the nucleobases. The photocleavage of plasmid pUC19 DNA was observed upon the irradiation of - with visible and red light, attributed to the sensitized generation of O by the complexes. These findings indicate that the bim ligand, together with pydppn, serves to shift the absorption of Ru(II) complexes to the photodynamic therapy window, 600-900 nm, and also extend the excited state lifetimes for the efficient production of cytotoxic singlet oxygen.
Topics: DNA; Coordination Complexes; Ruthenium; Plasmids; Photochemotherapy; Singlet Oxygen; Photosensitizing Agents; Molecular Structure; DNA Cleavage
PubMed: 38823006
DOI: 10.1021/acs.inorgchem.4c01665 -
The Journal of Evidence-based Dental... Jun 2024Despite phototherapy (in the form of photodynamic therapy (PDT)-mediated oxidative stress) being utilized in the management of oral potentially malignant disorders... (Meta-Analysis)
Meta-Analysis Review
OBJECTIVES
Despite phototherapy (in the form of photodynamic therapy (PDT)-mediated oxidative stress) being utilized in the management of oral potentially malignant disorders (OPMDs), the evidence of certainty remains unclear. Hence, this systematic review and meta-analysis (PROSPERO # CRD42021218748) is aimed to evaluate the clinical efficacy of PDT-induced oxidative stress in OPMDs METHODS: PubMed, Embase, Web of Science, Scopus, and Cochrane Library databases were searched without restriction of language or year of publication. In addition, gray literature was searched and a manual search was performed. Two independent reviewers screened all the studies, assessing data extraction, risk of bias and certainty of evidence. A narrative synthesis was carried out. For the meta-analysis, random effects were considered to determine the prevalence of a total and a partial remission (PR) of oral potentially malignant disorders (OPMDs). The certainty of evidence was explored using the Grading of Recommendations, Assessment, Development and Evaluation (GRADE) approach.
RESULTS
Twenty-three studies were included in the qualitative and quantitative syntheses. A total of 880 patients were included (564 males; 218 females) with an age range between 24 and 89-years-old. The results showed the prevalence of the total and partial remissions respectively for the following OPMLs: actinic cheilitis (AC): 69.9% and 2.4%; oral leukoplakia (OL): 44% and 36.9%; oral verrucous hyperplasia (OVH): 98.5%; oral erythroleukoplakia (OEL): 92.1% and 7.9%. The prevalence of no remission of OL was 18.8%.
CONCLUSIONS
PDT demonstrated significant results in clinical remission of OPMDs and most of the eligible studies have shown a total or a partial remission of the included lesions, but at a low or a very low certainty of evidence. Hence, further clinical studies with robust methodology are warranted to offer further validated data. Also, further evidence is required to understand further the mechanism of PDT-induced oxidative stress.
Topics: Aged; Aged, 80 and over; Female; Humans; Male; Cheilitis; Mouth Neoplasms; Oxidative Stress; Photochemotherapy; Photosensitizing Agents; Precancerous Conditions; Treatment Outcome; Adult; Middle Aged
PubMed: 38821659
DOI: 10.1016/j.jebdp.2023.101899 -
International Immunopharmacology Jul 2024The treatment of tumors still faces considerable challenges. While conventional treatments such as surgery, chemotherapy, and radiation therapy provide some curative... (Review)
Review
The treatment of tumors still faces considerable challenges. While conventional treatments such as surgery, chemotherapy, and radiation therapy provide some curative effects, their side effects and limitations highlight the importance of finding more precise treatment strategies. Aptamers have become an important target molecule in the field of drug delivery systems due to their good affinity and targeting, and they have gradually become an important link from basic research to clinical application. In this paper, we discussed the latest progress of aptamer-mediated nanodrugs, as well as aptamer-mediated photodynamic therapy, photothermal therapy, and immunotherapy strategies for tumor treatment, and explored the possibility of aptamer-mediated therapy for accurate tumor treatment. The purpose of this review is to provide novel insights for treating tumors with aptamer-mediated therapies by summarizing these innovative strategies, thereby ultimately enhancing the therapeutic efficacy for cancer patients.
Topics: Humans; Neoplasms; Aptamers, Nucleotide; Animals; Immunotherapy; Photochemotherapy; Drug Delivery Systems; Photothermal Therapy; Antineoplastic Agents
PubMed: 38820957
DOI: 10.1016/j.intimp.2024.112356