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Spectrochimica Acta. Part A, Molecular... Oct 2024The near-infrared (NIR) thermally activated delayed fluorescence (TADF) molecules hold practical application value in various fields, including biological imaging,...
The near-infrared (NIR) thermally activated delayed fluorescence (TADF) molecules hold practical application value in various fields, including biological imaging, anti-counterfeiting, sensors, telemedicine, photomicrography, and night vision display. These molecules have emerged as a significant development direction in organic electroluminescent devices, offering exciting possibilities for future technological advancements. Despite the remarkable potential of NIR-TADF molecules in various applications, the development of molecules that exhibit both long-wavelength emission and high efficiency remains a significant challenge. Herein, based on T-type and Y-type TADF molecules BCN-TPA and ECN-TPA, a novel X-type TADF molecule X-ECN-TPA is theoretically designed through a molecular fusion strategy. Utilizing first-principles calculations and the thermal vibration correlation function (TVCF) method, the photophysical properties and luminescent mechanisms of these three molecules in both solvent and solid (doped films) are revealed. A comparison of the luminescent properties of isomeric BCN-TPA and ECN-TPA shows that the enhanced luminescence efficiency of BCN-TPA in the solid states is attributed to higher radiative rates and lower non-radiative rates. Furthermore, compared to BCN-TPA and ECN-TPA, X-ECN-TPA exhibits significant conjugation extension, resulting in a pronounced redshift, reaching 831 nm and 813 nm in solvent and solid states, respectively. Importantly, molecular fusion significantly increases the transition dipole moment density between the donor and acceptor, leading to a substantial increase in radiative transition rates. Additionally, molecular fusion effectively reduces the energy gap between the first singlet excited state (S) and the first triplet excited state (T), facilitating the improvement of the reverse intersystem crossing (RISC) process. In addition, the calculation of Marcus formula shows that the triplet energy transfer from CBP to BCN-TPA, ECN-TPA and X-ECN-TPA is very effective. This work not only designs a novel efficient NIR-TADF molecule but also proposes a strategy for designing efficient NIR-TADF molecules. This principle offers unique insights for optimizing traditional molecular frameworks, opening up new possibilities for future advancements.
PubMed: 38795526
DOI: 10.1016/j.saa.2024.124500 -
Journal of Dental Sciences Jan 2024Integration of arts and humanities into dental education is important for developing holistically oriented dental professionals. This study aimed to survey dental...
BACKGROUND/PURPOSE
Integration of arts and humanities into dental education is important for developing holistically oriented dental professionals. This study aimed to survey dental students' perception of integrating arts and sciences into dental education with an innovative pedagogical approach through a photomicrograph competition.
MATERIALS AND METHODS
Students used digital imaging tools and creative approaches to capture photomicrographs that revealed hidden beauty in oral tissue sections. The photomicrograph works were posted online for scoring. The scoring activity, questionnaire survey, and complimentary comments of the participants were conducted online using a Google form.
RESULTS
The photomicrograph competition garnered a 24% participation rate from the 490 undergraduate dental students. Of the 116 participants, 65% agreed that the photomicrograph competition increased their interest in learning microscopic lessons, 87% very strongly or strongly supported to hold the photomicrograph competition every year, and 72% believed that the photomicrograph competition could be adopted to the dental students in all dental schools in Taiwan. The overall satisfaction rate of the photomicrograph competition was 91%. The free comments from all participants were generally positive towards this photomicrograph competition.
CONCLUSION
The photomicrograph competition shows the transformative potential of integrating arts and humanities into dental education. Through the lens of photomicrography, the hidden wonders in oral tissue sections have been unveiled. This photomicrograph competition, a pioneering endeavor with no precedent in English literature, has proven to be a resounding success that unites the realms of scientific inquiry and artistic expression, and may be a transformative agent in nurturing holistic dental professionals.
PubMed: 38303888
DOI: 10.1016/j.jds.2023.09.003 -
Neotropical Entomology Oct 2023This study is focused on the cricket leeches, Rhopalosomatidae, a family that is only poorly represented in entomological collections in Brazil. We provided a revised...
This study is focused on the cricket leeches, Rhopalosomatidae, a family that is only poorly represented in entomological collections in Brazil. We provided a revised and updated key to the genera occurring in Brazil with the major traits of genera illustrated through high-resolution photomicrography. Also, we provided a synopsis of genera, a list of the species currently recorded from Brazil, the first country records for Rhopalosoma minus Townes, 1977 and Rhopalosoma breelandi Townes, 1977, which increases the diversity of these wasps in the country. Additionally, we provided information and a brief discussion about collection methods, flotation, and abundance of specimens collected. Maps with the geographical distribution of the studied species based on the previous and new records are also provided.
PubMed: 37603231
DOI: 10.1007/s13744-023-01066-w -
Frontiers in Plant Science 2023Oil-based emulsion solution is a common pesticide formulation in agricultural spraying, and its spray characteristics are different from that of water spraying. The well...
INTRODUCTION
Oil-based emulsion solution is a common pesticide formulation in agricultural spraying, and its spray characteristics are different from that of water spraying. The well understanding of its spray characteristics is the theoretical basis to improve the pesticide spraying technology. The objective of the present study is to deepen the understanding of the spray characteristics of oil-based emulsion.
METHOD
In this paper, the spatial distribution characteristics of spray droplets of oil-based emulsion were captured visually using the high-speed photomicrography. On the basis of image processing method, the droplet size and distribution density of spray droplets at different spatial locations were analyzed quantitatively. The effects of nozzle configuration and emulsion concentration on spray structures and droplet spatial distribution were discussed.
RESULTS
Oil-based emulsion produced a special perforation atomization mechanism compared with water spray, which led to the increase of spray droplet size and distribution density. Nozzle configuration had a significant effect on oil-based emulsion spray, with the nozzle changed from ST110-01 to ST110-03 and ST110-05; the sheet lengths increased to 18 and 28 mm, respectively, whereas the volumetric median diameters increased to 51.19% and 76.00%, respectively. With emulsion concentration increased from 0.02% to 0.1% and 0.5%, the volumetric median diameters increased to 5.17% and 14.56%, respectively.
DISCUSSION
The spray droplet size of oil-based emulsion spray can be scaled by the equivalent diameter of discharge orifice of nozzles. The products of volumetric median diameters and corresponding surface tensions were nearly constant for the oil-based emulsion spray of different emulsion concentrations. It is expected that this research could provide theoretical support for improving the spraying technology of oil-based emulsion and increasing the utilization of pesticide.
PubMed: 37360725
DOI: 10.3389/fpls.2023.1183387 -
Journal of the American Association For... May 2023Hypodermic needles are sometimes reused in animal research settings to preserve the viability of and to conserve limited quantities of injected material. However, the...
Hypodermic needles are sometimes reused in animal research settings to preserve the viability of and to conserve limited quantities of injected material. However, the reuse of needles is strongly discouraged in human medicine to prevent inju- ries and the spread of infectious disease. No official guidelines prohibit needle reuse in veterinary medicine, although the practice may be discouraged. We hypothesized that reused needles would be significantly more blunt than unused needles and that reuse for additional injections would cause more animal stress. To test these ideas, we evaluated mice that were injected subcutaneously in the flank or mammary fat pad to generate cell line xenograft and mouse allograft models. Needles were reused up to 20 times, based on an IACUC-approved protocol. A subset of reused needles was digitally imaged to determine needle dullness based on the area of deformation from the secondary bevel angle; this parameter was not different between new needles and needles that had been reused 20 times. In addition, the number of times a needle was reused was not significantly related to audible mouse vocalization during injection. Finally, nest building scores for mice that were injected with a needle used 0 through 5 times were similar to those of mice injected with a needle had been used 16 through 20 times. Among the 37 reused needles that were tested, 4 were positive for bacterial growth; the only organisms cultured were spp. Contrary to our hypothesis, reusing needles for subcutaneous injections did not increase animal stress based on analysis of vocalization or nest building.
Topics: Humans; Animals; Mice; Needles; Vocalization, Animal; Photomicrography; Injections, Subcutaneous
PubMed: 36990673
DOI: 10.30802/AALAS-JAALAS-22-000094 -
Heliyon Dec 2022The formulation of niosomes is influenced by a number of variables, and these variables may eventually affect the formulation's outcome. One of the elements that can...
The formulation of niosomes is influenced by a number of variables, and these variables may eventually affect the formulation's outcome. One of the elements that can influence the physico-chemical properties of niosomes is the method used in preparation of the formulation. In this study, we established if various methods of preparation have any impact on the prepared vesicles when loaded with 5-fluorouracil. Thereafter, a real-time cell assay (an in vitro cytotoxicity test) against HCT-116 colon cancer cell lines was done on an optimised batch. 5-fluorouracil loaded niosomes were prepared with either Tween 60 or Span 60 by four different methods - namely thin film hydration (TFH), reverse phase evaporation (RPE), evaporation/sonication (EVP/SON), and the ethanol injection method (EIM). In vitro evaluations were done on the formulations, and these included particle size analysis, entrapment efficiency, scanning electron microscopy (SEM), photomicrography, drug release, polydispersity index, and Fourier transform infrared spectroscopy (FTIR). The effects of the preparation method and type of non-ionic surfactants on encapsulation efficiency, particle size, and in vitro drug release of the niosomes at pH 7.4 were evaluated. An cytotoxicity test (real time cell assay (RTCA)) against HCT-116 cells was carried out using the optimised formulation. Results showed physically stable formulations. The TFH method produced the smallest particle sizes (187 nm and 482 nm), while the EVP/SON method produced the largest particle sizes (4476 nm and 9111 nm). The Tween-based niosomes prepared by TFH or RPE had higher drug entrapment. The FTIR studies of niosomal formulations showed broad peaks at wavenumbers above 3000 cm, indicating strong hydrogen bonds. The RTCA showed 5-fluorouracil-loaded niosomes caused more sustained cell death compared to the pure drug and blank niosomes. The methods of preparation affected the particle size, polydispersity index, entrapment efficiency, and the physical stability of the vesicles. The thin film hydration method was more robust in the entrapped 5-fluorouracil and showed lower particle sizes when compared to all the other methods. RTCA showed sustained cell death in real time.
PubMed: 36582708
DOI: 10.1016/j.heliyon.2022.e12369 -
Zoological Studies 2022(Dufour, 1849) is the most widespread species of the planthopper subfamily Orgeriinae (Hemiptera: Fulgoromorpha: Dictyopharidae) in Europe, found from the Iberian...
(Dufour, 1849) is the most widespread species of the planthopper subfamily Orgeriinae (Hemiptera: Fulgoromorpha: Dictyopharidae) in Europe, found from the Iberian Peninsula to the Western Balkans. However, its diagnostically important genitalia and biology have been insufficiently described. We employ state-of-the-art synchrotron X-ray microtomography and photomicrography to re-describe , and to study the morphology of both its sexes in unprecedented detail. By examining specimens from across the distribution of , we find that they probably belong to a single, broadly distributed morphospecies. Our morphological examination allowed us to make inferences on its jumping mechanism and capacity for vibrational communication. We also record for the first time from Greece, further extending the range of this elusive species. Detailed information on the habitat of is also provided.
PubMed: 36568820
DOI: 10.6620/ZS.2022.61-51 -
Journal of Periodontal Research Dec 2022This study investigated the impact of colitis induced by dextran sulphate sodium (DSS)-induced colitis (DIC) on histopathological and immunological outcomes in the...
OBJECTIVE
This study investigated the impact of colitis induced by dextran sulphate sodium (DSS)-induced colitis (DIC) on histopathological and immunological outcomes in the periodontal tissues of Wistar rats.
BACKGROUND
Inflammatory bowel diseases (IBD) and periodontitis have been reported to present a bidirectional relationship. However, the inflammatory pathway that connects both diseases needs further investigation.
MATERIAL AND METHODS
Twenty-five male Wistar rats were allocated in four groups: unilateral ligature-induced periodontitis for 14 days: LIP (n = 7); dextran sulphate sodium-induced colitis only: DIC (n = 6); DIC + LIP (n = 6) and controls (n = 6). Digital images were obtained from the histological sections. In order to assess the attachment loss (AL), the linear distance between the cementoenamel junction (CEJ) and the alveolar bone crest was measured on the mesial root using histological photomicrography's ImageJ software. Immunological analyses of gingival tissues and plasma were performed by Bio-Plex Th1/Th2 Assay.
RESULTS
The DIC group showed inflammatory cells extending to the periodontal connective tissues, which contained significantly elevated expressions of IL-1α, IL-1β, IL-2, IL-6, IL-12, IL-13, GM-CSF, IFN-γ and TNF-α compared to controls. There was no significant difference in bone loss between controls and DIC. There were no significant histopathological differences between DIC + LIP and LIP. However, DIC + LIP presented a significantly lower IL-2 and IL-5 than the LIP group. There was no bone loss difference between LIP+DIC and LIP groups. DIC + LIP group presented significantly higher levels of GM-CSF in plasma.
CONCLUSION
DSS-induced colitis was associated with an overexpression of Th1/Th2- related cytokines in the gingival tissue.
Topics: Rats; Animals; Male; Rats, Wistar; Granulocyte-Macrophage Colony-Stimulating Factor; Dextran Sulfate; Interleukin-2; Colitis; Periodontitis; Cytokines; Disease Models, Animal
PubMed: 36253900
DOI: 10.1111/jre.13063 -
Parasite (Paris, France) 2022Accurate identification of insect species is an indispensable and challenging requirement for every entomologist, particularly if the species is involved in disease...
Accurate identification of insect species is an indispensable and challenging requirement for every entomologist, particularly if the species is involved in disease outbreaks. The European MediLabSecure project designed an identification (ID) exercise available to any willing participant with the aim of assessing and improving knowledge in mosquito taxonomy. The exercise was based on high-definition photomicrographs of mosquitoes (26 adult females and 12 larvae) collected from the western Palaearctic. Sixty-five responses from Europe, North Africa and the Middle East were usable. The study demonstrated that the responders were better at identifying females (82% correct responses) than larvae (63%). When the responders reported that they were sure of the accuracy of their ID, the success rate of ID increased (92% for females and 88% for larvae). The top three tools used for ID were MosKeyTool (72% of responders), the ID key following Becker et al. [2010. Mosquitoes and their control, 2nd edn. Berlin: Springer] (38%), and the CD-ROM of Schaffner et al. [2001. Les moustiques d'Europe: logiciel d'identification et d'enseignement - The mosquitoes of Europe: an identification and training programme. Montpellier: IRD; EID] (32%), while other tools were used by less than 10% of responders. Responders reporting the identification of mosquitoes using the MosKeyTool were significantly better (80% correct responses) than non-MosKeyTool users (69%). Most responders (63%) used more than one ID tool. The feedback from responders in this study was positive, with the exercise being perceived as halfway between educational training and a fun quiz. It raised the importance of further expanding training in mosquito ID for better preparedness of mosquito surveillance and control programmes.
Topics: Africa, Northern; Animals; Culicidae; Disease Outbreaks; Europe; Female; Humans; Larva; Mosquito Vectors
PubMed: 36200781
DOI: 10.1051/parasite/2022045 -
The Science of the Total Environment Nov 2022Pollen and spores have been identified as major airborne bio-particles inducing respiratory disorders including allergic rhinitis and atopic dermatitis among...
Pollen and spores have been identified as major airborne bio-particles inducing respiratory disorders including allergic rhinitis and atopic dermatitis among hypersensitive individuals. The present study was conducted with a view to investigating monthly depositional rate of atmospheric palynomorphs to determine the influence of the immediate vegetation on airborne pollen distribution; allergenic activities of dominant atmospheric pollen types at selected study locations in Taraba and Bauchi States, Northeastern Nigeria. Bioaerosols were collected using Tauber-like pollen traps and subjected to standard palynological treatment procedures, microscopy and photomicrography. Plant enumeration within the surrounding vegetation revealed that some airborne pollen types were produced by local plants at the study locations. Spores of Nephrolepis sp., Pteris sp. and a trilete fern, as well as diatoms were also recovered. Crude protein contents of some dominant pollen types; Borreria verticillata (L.) G.F.W. Meyer and Panicum maximum Jacq. for Taraba State; Leucaena leucocephala (Lam.) de Wit. and Terminalia catappa L. for Bauchi State, were quantified and extracted to sensitize Mus musculus mice for serology (ELISA) and haematology (differential and total white blood cell counts). Statistical significance was tested and recorded in the correlation between levels of serological and haematological parameters elicited by each test group; differences between levels of these parameters elicited by each test group and those of the control, as well as at varying sensitization periods. In the Leucaena leucocephala test group, swollen body and histopathological morbid features showing more extensive areas of inflammatory cells and alveoli filled with fluid in the lungs, were recorded in two dead M. musculus, respectively. The study revealed that all the tested pollen types are possible allergens at the study locations, establishing a complexity of interaction among allergy mediators at varied periods of mice sensitization and forming a paradigm of human immune response to the different pollen allergens.
Topics: Allergens; Animals; Humans; Hypersensitivity; Mice; Nigeria; Plants; Pollen; Rhinitis, Allergic, Seasonal; Spores
PubMed: 35780899
DOI: 10.1016/j.scitotenv.2022.157076