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International Journal of Gynecological... Jan 2019Since other genital infections enhance HIV susceptibility by inducing inflammation and evidence suggests that the vaginal microbiome plays a functional role in the...
OBJECTIVES
Since other genital infections enhance HIV susceptibility by inducing inflammation and evidence suggests that the vaginal microbiome plays a functional role in the persistence or regression of high-risk human papillomavirus (HPV) infections, we investigated the relationship between the composition of the vaginal microbiota and the risk of high-risk HPV infection.
METHODS
The study included 151 healthy women (65 HPV-positive and 86 HPV-negative) aged 20-65 at enrollment. Total genome DNA from samples was extracted using the hexadecyltrimethylammonium bromide (CTAB) CTAB method. The vaginal microbiota composition was determined by sequencing barcoded 16S rDNA gene fragments (V4) on Illumina HiSeq2500.
RESULTS
Of the 30 most abundant bacteria at the genus level, we found only six bacteria with a statistical difference between HPV-positive and HPV-negative women: , , , , , and was the predominant genus and was detected in all women, but there was no significant difference between the two groups for , , and . Furthermore, we found 26 types of bacteria with a statistical difference at the species level between the two groups. Anaerobic bacteria such as , , and were found significantly more frequently in HPV-positive women, which is the most important finding of our study.
CONCLUSION
Our findings suggest a possible role for the composition of the vaginal microbiota as a modifier of high-risk HPV infection, and specific microbiota species may serve as sensors for changes in the cervical microenvironment associated with high-risk HPV infection. The exact molecular mechanism of the vaginal microbiota in the course of high-risk HPV infection and cervical neoplasia should be further explored. Future research should include intervention in the composition of the vaginal microbiota to reverse the course of high-risk HPV infection and the natural history of cervical neoplasia.
Topics: Adult; Bacteria; China; Female; Follow-Up Studies; Humans; Middle Aged; Papillomaviridae; Papillomavirus Infections; Prognosis; Risk Factors; Tumor Microenvironment; Vagina; Young Adult
PubMed: 30640680
DOI: 10.1136/ijgc-2018-000032 -
Anaerobe Dec 2017Prevotella buccae is a Gram-negative anaerobic rod involved in some human infections. We report the first case of an infected breast cyst in a non-puerperal women due to...
Prevotella buccae is a Gram-negative anaerobic rod involved in some human infections. We report the first case of an infected breast cyst in a non-puerperal women due to this pathogen. A 53-year-old woman presented with pain and a cystic lesion in the right breast. Culture of abscess drainage resulted in isolation of P. buccae. High level of resistance to metronidazole was documented. Treatment with drainage and amoxicillin-clavulanate was established, and improvement of this infection was observed.
Topics: Abscess; Bacteroidaceae Infections; Breast Cyst; Combined Modality Therapy; Drainage; Drug Resistance, Bacterial; Female; Humans; Mass Spectrometry; Mastitis; Metronidazole; Microbial Sensitivity Tests; Middle Aged; Prevotella; Treatment Outcome
PubMed: 28866113
DOI: 10.1016/j.anaerobe.2017.08.015 -
Veterinary Microbiology May 2017Periodontitis is a polymicrobial infectious disease that causes occlusion change, tooth loss, difficulty in rumination, and premature culling of animals. This study...
Periodontitis is a polymicrobial infectious disease that causes occlusion change, tooth loss, difficulty in rumination, and premature culling of animals. This study aimed to detect species of the genera Porphyromonas and Prevotella present in the periodontal pocket of sheep with lesions deeper than 5mm (n=14) and in the gingival sulcus of animals considered periodontally healthy (n=20). The presence of microorganisms was evaluated by polymerase chain reaction (PCR) using specific primers for Porphyromonas asaccharolytica, Porphyromonas endodontalis, Porphyromonas gingivalis, Porphyromonas gulae, Prevotella buccae, Prevotella intermedia, Prevotella loescheii, Prevotella melaninogenica, Prevotella nigrescens, Prevotella oralis, and Prevotella tannerae. Prevalence and risk analysis were performed using Student's t-test and Spearman's correlation. Among the Prevotella and Porphyromonas species detected in the periodontal lesions of sheep, P. melaninogenica (85.7%), P. buccae (64.3%), P. gingivalis (50%), and P. endodontalis (50%) were most prevalent. P. gingivalis (15%) and P. oralis (10%) prevailed in the gingival sulcus. P. gulae and P. tannerae were not detected in the 34 samples studied. Data evaluation by t-test verified that occurrence of P. asaccharolytica, P. endodontalis, P. gingivalis, P. buccae, P. intermedia, P. melalinogenica, and P. nigrescens correlated with sheep periodontitis. The findings of this study will be an important contribution to research on pathogenesis of sheep periodontitis and development of its control measures.
Topics: Animals; Bacteria, Anaerobic; Biofilms; Periodontal Pocket; Periodontitis; Polymerase Chain Reaction; Porphyromonas; Prevotella; Sheep; Sheep Diseases
PubMed: 28619155
DOI: 10.1016/j.vetmic.2017.03.032 -
Vascular and Endovascular Surgery Aug 2017Descending necrotizing mediastinitis (DNM) is a life-threatening disease which often develops from a purulent infection of the oral cavity and is associated with high...
Descending necrotizing mediastinitis (DNM) is a life-threatening disease which often develops from a purulent infection of the oral cavity and is associated with high rates of mortality. Here we report the case of a young patient who died from an aortic arch rupture in context of DNM developed from an odontogenic infection caused by Prevotella buccae. Based on the current knowledge on this very rare vascular complication, we discuss factors that may have contributed to this fatal issue and future issues to optimize care provided to patients.
Topics: Anti-Bacterial Agents; Aorta, Thoracic; Aortic Rupture; Bacteroidaceae Infections; Debridement; Drainage; Fatal Outcome; Female; Focal Infection, Dental; Humans; Mediastinitis; Molar, Third; Necrosis; Prevotella; Thoracotomy; Tomography, X-Ray Computed; Tooth Extraction; Treatment Outcome; Young Adult
PubMed: 28618847
DOI: 10.1177/1538574417715193 -
Brazilian Dental Journal 2016The objective of this study was to investigate the bacterial composition present in root canals of teeth associated with acute apical abscess by molecular identification...
The objective of this study was to investigate the bacterial composition present in root canals of teeth associated with acute apical abscess by molecular identification (16S rRNA) of cultivable bacteria. Two hundred and twenty strains isolated by culture from 20 root canals were subjected to DNA extraction and amplification of the 16S rRNA gene (PCR), followed by sequencing. The resulting nucleotide sequences were compared to the GenBank database from the National Center of Biotechnology Information through BLAST. Strains not identified by sequencing were submitted to clonal analysis. The association of microbiological findings with clinical features and the association between microbial species were also investigated. Fifty-nine different cultivable bacteria were identified by 16S rRNA gene sequencing, belonging to 6 phyla, with an average number of 6 species per root canal. Molecular approaches allowed identification of 99% of isolates. The most frequently identified bacteria were Prevotella spp., Pseudoramibacter alactolyticus, Parvimonas micra, Dialister invisus, Filifactor alocis, and Peptostreptococcus stomatis. Positive association was found between Prevotella buccae and Pseudoramibacter alactolyticus and between Parvimonas micra and Prevotella nigrescens (both p<0.05). It was concluded that the microbiota of infected root canals associated with acute apical abscess is diverse and heterogeneous, composed mainly of anaerobic Gram-negative bacteria, with the great majority belonging to the phyla Firmicutes and Bacteroidetes.
Topics: Acute Disease; Bacteria; Humans; Periapical Abscess; Phylogeny; RNA, Ribosomal, 16S
PubMed: 27224567
DOI: 10.1590/0103-6440201600715 -
The Journal of Reproductive Medicine 2015Retropharyngeal abscess is extremely uncommon in adults and rare in pregnancy. A MEDLINE search yielded only 1 reported case during pregnancy. Likewise, sepsis caused by...
BACKGROUND
Retropharyngeal abscess is extremely uncommon in adults and rare in pregnancy. A MEDLINE search yielded only 1 reported case during pregnancy. Likewise, sepsis caused by prevotella buccae, a bacteria associated with more benign conditions such as bacterial vaginosis or dental infections, is also rare, with no reported cases during pregnancy.
CASE
We report a case of retropharyngeal abscess complicated by Prevotella buccae septicemia during pregnancy.
CONCLUSION
Retropharyngeal abscess should be suspected in a pregnant patient who presents with sore throat, dyspnea, and dysphagia, especially when preceded by dental treatment or symptoms. If confirmed, antibiotic treatment should cover the usual pathogens associated with retropharyngeal abscess, including Pre- votella species.
Topics: Adult; Bacteroidaceae Infections; Female; Humans; Pregnancy; Pregnancy Complications, Infectious; Prevotella; Retropharyngeal Abscess; Sepsis
PubMed: 25745759
DOI: No ID Found -
Anaerobe Oct 2015The purpose of this study was to investigate the susceptibility of a series of 33 Prevotella strains isolated from patients with abscesses in the head and neck spaces,...
The purpose of this study was to investigate the susceptibility of a series of 33 Prevotella strains isolated from patients with abscesses in the head and neck spaces, presented to one Romanian hospital. The Etest was applied to determine the value of the minimum inhibitory concentrations for: penicillin G, ampicillin, amoxicillin-clavulanate, metronidazole and clindamycin. In addition, the beta-lactamase activity was detected by the chromogenic cephalosporin disc method. The results indicated that 11 isolates were resistant to both penicillin G and ampicillin due to the beta-lactamase production. All the 33 Prevotella strains were susceptible to the other 3 antimicrobial agents tested, except for only one penicillin G - ampicillin resistant isolate of Prevotella buccae (MIC > 32 and MIC = 12 mg/L, respectively), which showed high resistance to clindamycin (MIC > 256 mg/L) too. Our data underline the necessity for antimicrobial testing including monitoring of beta-lactamase production in cases of oro-maxillo-facial mixed anaerobic infections where antimicrobial treatment is required in addition to the surgical drainage. The results of the study indicated that amoxicillin-clavulanate, like metronidazole, was fully active against the tested Prevotella strains. However, local and multicentre surveys on drug resistance among the clinically significant anaerobic isolates should be carried out periodically.
Topics: Abscess; Anti-Bacterial Agents; Bacterial Proteins; Bacteroidaceae Infections; Drug Resistance, Bacterial; Head; Humans; Microbial Sensitivity Tests; Neck; Prevotella; Romania; beta-Lactamases
PubMed: 25463968
DOI: 10.1016/j.anaerobe.2014.10.006