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Antonie Van Leeuwenhoek Dec 2015In the context of studying the influence of N-fertilization on N2 and N2O flux rates in relation to the soil bacterial community composition in fen peat grassland, a...
In the context of studying the influence of N-fertilization on N2 and N2O flux rates in relation to the soil bacterial community composition in fen peat grassland, a group of bacterial strains was isolated that performed dissimilatory nitrate reduction to ammonium and concomitantly produced N2O. The amount of nitrous oxide produced was influenced by the C/N ratio of the medium. The potential to generate nitrous oxide was increased by higher availability of nitrate-N. Phylogenetic analysis based on the 16S rRNA and the rpoB gene sequences demonstrated that the investigated isolates belong to the genus Proteus, showing high similarity with the respective type strains of Proteus vulgaris and Proteus penneri. DNA-DNA hybridization studies revealed differences at the species level. These differences were substantiated by MALDI-TOF MS analysis and several distinct physiological characteristics. On the basis of these results, it was concluded that the soil isolates represent a novel species for which the name Proteus terrae sp. nov. (type strain N5/687(T) =DSM 29910(T) =LMG 28659(T)) is proposed.
Topics: Ammonia; Bacterial Typing Techniques; Carbon; Cluster Analysis; Culture Media; DNA, Bacterial; DNA, Ribosomal; DNA-Directed RNA Polymerases; Deinococcus; Molecular Sequence Data; Nitrates; Nitrogen; Nitrous Oxide; Nucleic Acid Hybridization; Phylogeny; Proteus; RNA, Ribosomal, 16S; Sequence Analysis, DNA; Soil Microbiology; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
PubMed: 26437638
DOI: 10.1007/s10482-015-0601-5 -
Carbohydrate Research Apr 2015Proteus penneri is an opportunistic pathogen, which may cause severe diseases, most frequently urinary tract infections in immunocompromised patients. P. penneri Br 114...
Proteus penneri is an opportunistic pathogen, which may cause severe diseases, most frequently urinary tract infections in immunocompromised patients. P. penneri Br 114 exhibiting a good swarming growth ability as an S-form strain was isolated from a wound of a patient in Łódź, Poland. Serological studies using ELISA and Western blotting and chemical analyses along with (1)H and (13)C NMR spectroscopy showed that the O-antigen (O-polysaccharide) of this strain is unique among the known Proteus serotypes O1-O79. It possesses a linear pentasaccharide repeating unit containing a partially O-acetylated amide of D-glucuronic acid (GlcA) with L-serine having the following structure: [structure: see text]. These data are a basis for creating a new Proteus serogroup, O80, so far represented by the single Br 114 isolate. The O80 is the 21st O-serogroup containing P. penneri strains and the fourth serogroup based on Proteus spp. clinical isolates from Łódź, Poland.
Topics: Carbohydrate Sequence; Humans; Magnetic Resonance Spectroscopy; Male; Middle Aged; O Antigens; Poland; Proteus Infections; Proteus penneri; Serotyping
PubMed: 25771295
DOI: 10.1016/j.carres.2015.02.003