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Frontiers in Microbiology 2016In continuation of our search for new bioactive compounds from soil microbes, a fluorescent Pseudomonas strain isolated from paddy field soil of Kuttanad, Kerala, India...
In continuation of our search for new bioactive compounds from soil microbes, a fluorescent Pseudomonas strain isolated from paddy field soil of Kuttanad, Kerala, India was screened for the production of bioactive secondary metabolites. This strain was identified as Pseudomonas mosselii through 16S rDNA gene sequencing followed by BLAST analysis and the bioactive metabolites produced were purified by column chromatography (silica gel) and a pure bioactive secondary metabolite was isolated. This bioactive compound was identified as Pseudopyronine B by NMR and HR-ESI-MS. Pseudopyronine B recorded significant antimicrobial activity especially against Gram-positive bacteria and agriculturally important fungi. MTT assay was used for finding cell proliferation inhibition, and Pseudopyronine B recorded significant antitumor activity against non-small cell lung cancer cell (A549), and mouse melanoma cell (B16F10). The preliminary MTT assay results revealed that Pseudopyronine B recorded both dose- and time-dependent inhibition of the growth of test cancer cell lines. Pseudopyronine B induced apoptotic cell death in cancer cells as evidenced by Acridine orange/ethidium bromide and Hoechst staining, and this was further confirmed by flow cytometry analysis using Annexin V. Cell cycle analysis also supports apoptosis by inducing G2/M accumulation in both A549 and B16F10 cells. Pseudopyronine B treated cells recorded significant up-regulation of caspase 3 activity. Moreover, this compound recorded immunomodulatory activity by enhancing the proliferation of lymphocytes. The production of Pseudopyronine B by P. mosselii and its anticancer activity in A549 and B16F10 cell lines is reported here for the first time. The present study has a substantial influence on the information of Pseudopyronine B from P. mosselii as potential sources of novel drug molecule for the pharmaceutical companies, especially as potent antimicrobial and anticancer agent.
PubMed: 27617005
DOI: 10.3389/fmicb.2016.01307 -
Effect of plant growth-promoting bacteria on the growth and fructan production of Agave americana L.Brazilian Journal of Microbiology :... 2016The effect of plant growth-promoting bacteria inoculation on plant growth and the sugar content in Agave americana was assessed. The bacterial strains ACO-34A, ACO-40,...
The effect of plant growth-promoting bacteria inoculation on plant growth and the sugar content in Agave americana was assessed. The bacterial strains ACO-34A, ACO-40, and ACO-140, isolated from the A. americana rhizosphere, were selected for this study to evaluate their phenotypic and genotypic characteristics. The three bacterial strains were evaluated via plant inoculation assays, and Azospirillum brasilense Cd served as a control strain. Phylogenetic analysis based on the 16S rRNA gene showed that strains ACO-34A, ACO-40 and ACO-140 were Rhizobium daejeonense, Acinetobacter calcoaceticus and Pseudomonas mosselii, respectively. All of the strains were able to synthesize indole-3-acetic acid (IAA), solubilize phosphate, and had nitrogenase activity. Inoculation using the plant growth-promoting bacteria strains had a significant effect (p<0.05) on plant growth and the sugar content of A. americana, showing that these native plant growth-promoting bacteria are a practical, simple, and efficient alternative to promote the growth of agave plants with proper biological characteristics for agroindustrial and biotechnological use and to increase the sugar content in this agave species.
Topics: Agave; Bacteria; Fructans; Genotype; Phenotype; Phylogeny; Plant Growth Regulators; RNA, Ribosomal, 16S
PubMed: 27268113
DOI: 10.1016/j.bjm.2016.04.010 -
Genome Announcements Jun 2015We sequenced the complete genome of the isolate Pseudomonas sp. CCOS 191. This strain is able to dissolve phosphate minerals and form cyanide. The genome sequence is...
We sequenced the complete genome of the isolate Pseudomonas sp. CCOS 191. This strain is able to dissolve phosphate minerals and form cyanide. The genome sequence is used to establish the phylogenetic relationship of this species.
PubMed: 26067963
DOI: 10.1128/genomeA.00616-15 -
Journal of Biotechnology Dec 2014Pseudomonas mosselii strain SJ10 is a caprolactam-degrading bacterium belonging to the class Gammaproteobacteria, which was isolated from wastewater of the nylon 6...
Pseudomonas mosselii strain SJ10 is a caprolactam-degrading bacterium belonging to the class Gammaproteobacteria, which was isolated from wastewater of the nylon 6 producing Seongseo industrial complex in Daegu, Republic of Korea. Here, we report the complete genome sequence of the strain, providing genetic information for biodegradation of aromatic compounds.
Topics: Base Sequence; Caprolactam; DNA, Bacterial; Genome, Bacterial; Industrial Waste; Molecular Sequence Data; Polymers; Pseudomonas; Sequence Analysis, DNA; Wastewater
PubMed: 25449545
DOI: 10.1016/j.jbiotec.2014.10.024 -
Environmental Microbiology Reports Apr 2015Bacteria of the genus Pseudomonas are becoming increasing well known for their ability to produce a wide range of antimicrobial compounds. In a large-scale screening for...
Bacteria of the genus Pseudomonas are becoming increasing well known for their ability to produce a wide range of antimicrobial compounds. In a large-scale screening for antibiotic producers, we identified a soil isolate that uses 4-hydroxyphenylacetate as the sole carbon source, Pseudomonas sp. strain 250J, which produces cyclic lipodepsipeptides of the xantholysin family during the stationary phase of growth. The closest relatives of this strain are Pseudomonas mosselii, Pseudomonas soli and Pseudomonas entomophila. Sequencing of the 250J genome allowed us to find the genes relevant to antibiotic production, those which allow utilization of 4-hydroxyphenylacetate as a sole carbon source and a set of genes potentially involved in biocontrol.
Topics: Anti-Bacterial Agents; DNA, Bacterial; Depsipeptides; Genome, Bacterial; Metabolic Networks and Pathways; Molecular Sequence Data; Phenylacetates; Pseudomonas; Sequence Analysis, DNA; Soil Microbiology
PubMed: 25403737
DOI: 10.1111/1758-2229.12245 -
Systematic and Applied Microbiology Sep 2014A chemoorganotrophic Gram-negative bacterium was isolated by means of a diffusion sandwich system from a soil sample from the Sierra Nevada National Park, Spain. Strain...
A chemoorganotrophic Gram-negative bacterium was isolated by means of a diffusion sandwich system from a soil sample from the Sierra Nevada National Park, Spain. Strain F-279,208(T) was oxidase and catalase positive, strictly aerobic, non-spore-forming and motile by single polar flagellum. Phylogenetic analysis of the 16S rRNA, gyrB, rpoB and rpoD genes revealed that strain F-279,208(T) belongs to the Pseudomonas putida group with Pseudomonas mosselii and Pseudomonas entomophila as its closest relatives. DNA-DNA hybridization assays and phenotypic traits confirmed that this strain belongs to a novel species of the genus Pseudomonas, for which the name Pseudomonas soli sp. nov. is proposed. The type strain is F-279,208(T) (=DSM 28043(T)=LMG 27941(T)), and during fermentation it produces xantholysins, a family of lipodepsipeptides. The major compound, xantholysin A, showed an interesting activity in a RCC4 kidney tumor cell line with inactivation of VHL linked with the HIF pathway, without any cytotoxic effects against other human tumor cell lines tested including, liver, pancreas and breast.
Topics: Cell Line, Tumor; Genes, Bacterial; Humans; Molecular Sequence Data; Phenotype; Phylogeny; Pseudomonas; RNA, Ribosomal, 16S; Soil Microbiology
PubMed: 25097020
DOI: 10.1016/j.syapm.2014.07.003 -
Journal of Bioscience and Bioengineering Aug 2014The polyhydroxyalkanoate (PHA) production and growth of Pseudomonas mosselii TO7, a newly isolated Pseudomonas species from the wastewater of a vegetable oil...
The polyhydroxyalkanoate (PHA) production and growth of Pseudomonas mosselii TO7, a newly isolated Pseudomonas species from the wastewater of a vegetable oil manufacturing facility, was analyzed. Phenotypic analysis and phylogenetic analysis of the 16S rRNA gene revealed that it is closely related to Pseudomonas mosselii. In the presence of palm kernel and soybean oils, P. mosselii TO7 produced up to 50% cell dry weight (CDW) medium-chain-length (MCL) PHAs comprising high poly(3-hydroxyoctanoate) (P(3HO)) content; P(3HO) content increased to 45% CDW when grown in octanoate using a single-step culture process. The PHA monomer was identified by (13)C nuclear magnetic resonance spectroscopy. The average molecular weight and polydispersity index of PHA were 218.30 ± 31.73 and 2.21 ± 0.18, respectively. The PHA produced by P. mosselii TO7 in the presence of palm kernel oil had two melting temperature (Tm) values of 37.2°C and 55.7°C with melting enthalpy (ΔHm) values of 51.09 J g(-1) and 26.57 J g(-1), respectively. Inhibition analyses using acrylic and 2-bromooctanoic acids revealed β-oxidation as the primary pathway for MCL-PHA biosynthesis using octanoic acid. Moreover, Pseudomonas putida GPp104 PHA(-), harboring the PHA synthase genes of P. mosselii (phaC1pm and phaC2pm) was used for heterologous expression, which demonstrated that phaC1pm is the main PHA synthesis enzyme, and 3-hydroxyoctanoyl-CoA is its major substrate. This was the first report of a P. mosselii TO7 isolate producing high-yield P(3HO) through utilization of plant oils.
Topics: Acyltransferases; Bacterial Proteins; Caprylates; Lipid Metabolism; Molecular Typing; Phylogeny; Plant Oils; Polyhydroxyalkanoates; Pseudomonas; RNA, Ribosomal, 16S; Transition Temperature; Wastewater; Water Microbiology
PubMed: 24630613
DOI: 10.1016/j.jbiosc.2014.01.012