-
Clinical Chemistry and Laboratory... Jun 2024Conventional autoverification rules evaluate analytes independently, potentially missing unusual patterns of results indicative of errors such as serum contamination by...
OBJECTIVES
Conventional autoverification rules evaluate analytes independently, potentially missing unusual patterns of results indicative of errors such as serum contamination by collection tube additives. This study assessed whether multivariate anomaly detection algorithms could enhance the detection of such errors.
METHODS
Multivariate Gaussian, k-nearest neighbours (KNN) distance, and one-class support vector machine (SVM) anomaly detection models, along with conventional limit checks, were developed using a training dataset of 127,451 electrolyte, urea, and creatinine (EUC) results, with a 5 % flagging rate targeted for all approaches. The models were compared with limit checks for their ability to detect atypical EUC results from samples spiked with additives from collection tubes: EDTA, fluoride, sodium citrate, or acid citrate dextrose (n=200 per contaminant). The study additionally assessed the ability of the models to identify 127,449 single-analyte errors, a potential weakness of multivariate models.
RESULTS
The KNN distance and SVM models outperformed limit checks for detecting all contaminants (p-values <0.05). The multivariate Gaussian model did not surpass limit checks for detecting EDTA contamination but was superior for detecting the other additives. All models surpassed limit checks for identifying single-analyte errors, with the KNN distance model demonstrating the highest overall sensitivity.
CONCLUSIONS
Multivariate anomaly detection models, particularly the KNN distance model, were superior to the conventional approach for detecting serum contamination and single-analyte errors. Developing multivariate approaches to autoverification is warranted to optimise error detection and improve patient safety.
PubMed: 38863349
DOI: 10.1515/cclm-2024-0484 -
ACS Applied Materials & Interfaces Jun 2024Layer-by-layer (LbL) self-assembly of oppositely charged polyelectrolytes (PEs) is usually performed on a conventional ultrafiltration base substrate (negative zeta...
Layer-by-layer (LbL) self-assembly of oppositely charged polyelectrolytes (PEs) is usually performed on a conventional ultrafiltration base substrate (negative zeta potential) by depositing a cationic PE as a first layer. Herein, we report the facile and fast formation of high performance molecular selective membrane by the nonelectrostatic adsorption of anionic PE on the polyvinylidene fluoride (PVDF, zeta potential -17 mV) substrate followed by the electrostatic LbL assembly. Loose nanofiltration membranes have been prepared via both concentration-polarization (CP-LbL, under applied pressure) driven and conventional (C-LbL, dipping) LbL self-assembly. When the first layer is poly(styrene sodium) sulfonic acid, the LbL assembled membrane contains free -SO groups and exhibits higher rejection of NaSO and lower rejection of MgCl. The reversal of salt rejection occurs when the first layer is quaternized polyvinyl imidazole (PVIm-Me). The membrane (five layers) prepared by first depositing PStSONa shows higher rejection of several dyes (97.9 to >99.9%), higher NaCl to dye separation factor (52-1800), and higher dye antifouling performance as compared to the membrane prepared by first depositing PVIm-Me (97.5-99.5% dye rejection, separation factor ∼40-200). However, the C-LbL membrane requires a longer time of self-assembly or higher PE concentration to reach a performance close to the CP-LbL membranes. The membranes exhibit excellent pressure, pH (3-12), and salt (60 g L) stability. This work provides an insight for the construction of low fouling and high-performance membranes for the fractionation of dye and salt based on the LbL self-assembly sequence.
PubMed: 38861705
DOI: 10.1021/acsami.4c06169 -
ACS Omega Jun 2024Nonclassical crystallization represents an innovative pathway that utilizes nanoparticles, enabling the generation of single crystals, going beyond a classical mechanism...
Nonclassical crystallization represents an innovative pathway that utilizes nanoparticles, enabling the generation of single crystals, going beyond a classical mechanism dependent on atoms, ions, or molecules. Our investigation has revealed hierarchical structures emerging via the aggregation and fusion of primary silicon quantum dots (SiQDs). In contrast to the classical ion-by-ion crystallization process, the primary SiQDs initially undergo aggregation, followed by fusion and their subsequent crystallization, leading to the ultrafast crystal growth of sodium hexafluorosilicate (SHFS) microrods with diverse morphologies. A comprehensive fluorescence microscopy study is performed to examine the mechanism of microrod formation through the primary aggregation and fusion of SiQDs at room temperature in the presence of hydrogen fluoride (HF). The different concentrations of HF play a crucial role in the formation of flower-, block-, and hexagonal-shaped SHFS microrods. However, the presence of a high-concentration HF causes a reduction in microrod size, elucidated through a range of analytical and spectroscopic techniques.
PubMed: 38854570
DOI: 10.1021/acsomega.4c02952 -
American Journal of Orthodontics and... Jun 2024Interproximal reduction (IPR) damages the caries protective superficial layer of the enamel, making the enamel surface prone to caries because of the increase in surface...
INTRODUCTION
Interproximal reduction (IPR) damages the caries protective superficial layer of the enamel, making the enamel surface prone to caries because of the increase in surface roughness. Remineralizing solutions can help in preventing these undesirable side effects. Therefore, this study aimed to compare the effect of nanohydroxyapatite (nHAp) and sodium fluoride (NaF) application on enamel remineralization after IPR and to evaluate changes in surface roughness, composition, and microhardness of the treated enamel.
METHODS
A total of 25 patients with Angle's Class I malocclusion, requiring 4 premolar extractions, were selected and randomly divided into 5 groups (n = 5). Group 1 served as the control. In group 2, the extraction of premolars was done immediately after IPR, and in group 3, the extraction of premolars was done 3 months after IPR. In group 4, the extraction of premolars was performed 3 months after IPR with weekly application of nHAp serum. In group 5, the extraction of premolars was performed 3 months after IPR, along with once-a-month application of NaF varnish. The proximal reduction of premolars in all the groups was done using Strauss IPR burs (Strauss Diamond Instruments, Palm Coast, Fla). The extracted teeth were sectioned, and the enamel surfaces were subjected to energy-dispersive x-ray spectroscopy to evaluate elemental composition. Vicker's microhardness test was used to evaluate enamel hardness and atomic force microscopy for enamel surface roughness. Descriptive statistics were calculated for the 5 groups using a 1-way analysis of variance, and Tukey's multiple post-hoc test was used for intergroup comparison.
RESULTS
Calcium-to-phosphorous ratio, enamel microhardness, and surface roughness were found to be closest to untouched enamel in patients treated with nHAp, followed by patients who were treated with NaF. A lower calcium-to-phosphorous ratio and weakened and roughest enamel surface was seen in teeth, which were extracted immediately after IPR.
CONCLUSIONS
Among the remineralizing agents tested, nHAp serum can be recommended for better remineralization of enamel surfaces after IPR.
PubMed: 38852104
DOI: 10.1016/j.ajodo.2024.05.009 -
Journal of Dentistry Jun 2024The present study aimed to evaluate the effectiveness of bioactive glass (BAG) in preventing dental erosion in primary teeth.
OBJECTIVES
The present study aimed to evaluate the effectiveness of bioactive glass (BAG) in preventing dental erosion in primary teeth.
METHODS
Enamel and dentin specimens (2 × 2 × 2 mm) were obtained from extracted primary teeth, which were randomly divided into the following groups based on the pretreatments (n = 12): DW (deionized water), NaF (2 % sodium fluoride), 2BAG (2 % BAG), 4BAG (4 % BAG), 6BAG (6 % BAG), and 8BAG (8 % BAG). The specimens were immersed in the respective solutions for 2 min and subjected to in vitro erosive challenges (4 × 5 min/d) for 5 d. The erosive enamel loss (EEL), erosive dentin loss (EDL), and the thickness of the demineralized organic matrix (DOM) were measured using a contact profilometer. The surface microhardness (SMH) was measured, and the percentage of SMH loss (%SMHL) was calculated. The surface morphology and mineral composition were evaluated by scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDS), respectively.
RESULTS
After the erosive challenges, the EEL, EDL, and%SMHL of the 2BAG, 4BAG, 6BAG, and 8BAG groups significantly reduced, with the greatest reduction was observed in the 6BAG (EEL: 6.5 ± 0.2 μm;%SMHL in enamel: 12.8 ± 2.6; EDL: 7.9 ± 0.3 μm; %SMHL in dentin: 22.1 ± 2.7) and 8BAG groups (EEL: 6.4 ± 0.4 μm;%SMHL in enamel: 11.0 ± 1.9; EDL: 7.8 ± 0.5 μm; %SMHL in dentin: 22.0 ± 2.5) (P < 0.05). With increasing BAG concentrations, the number of surface deposits containing Ca, P, and Si increased.
CONCLUSIONS
6BAG was the most effective for preventing dental erosion in primary teeth and showed a particularly strong potential for dentin erosion prevention.
CLINICAL SIGNIFICANCE
Bioactive glass, especially at a 6 % concentration, has proven effective in reducing erosive tooth wear and surface microhardness loss while also protecting demineralized organic matrix in primary dentin.
PubMed: 38849053
DOI: 10.1016/j.jdent.2024.105109 -
International Journal of Biological... Jun 2024An eco-friendly macroparticle biochar (BC)-based Ce(III)-La(III) crosslinked sodium alginate (SA) hybrid hydrogel (BC/Ce-SA-La) was synthesized by droplet polymerization...
An eco-friendly macroparticle biochar (BC)-based Ce(III)-La(III) crosslinked sodium alginate (SA) hybrid hydrogel (BC/Ce-SA-La) was synthesized by droplet polymerization and characterized by SEM-EDS, XRD, FTIR, UV-Vis and XPS. The effects of dosage, pH, contact time, temperature and coexisting ions on the F ions removal by hybrid hydrogel, and the adsorption performance, interaction mechanism and reusability were investigated. The results demonstrate that the composite has a fancy wrinkle structure with a particle size of about 1.8 mm and abundant porosity on the surface. The removal rate of F ions by BC/Ce-SA-La reached 90.2 % under the conditions of pH 2.0, 200 min of contact time and 298 K. The adsorption behavior was perfectly explained by Langmuir model, and the maximum adsorption capacity reached 129 mg/g. The adsorption process was an endothermic spontaneous reaction and followed Pseudo-second-order rate model. The strong adsorption was attributed to multi-interactions including complexation, hydrogen bonding and electrostatic adsorption between the composite and F ions. Coexisting ions hardly interfered with the adsorption of F ions by BC/Ce-SA-La except for a slight effect of phosphate. The composite after F ion adsorption was easily separated and could be reused at least three times. BC/Ce-SA-La is a cost-effective and promising granular biosorbent.
Topics: Charcoal; Alginates; Adsorption; Fluorides; Hydrogels; Water Pollutants, Chemical; Water Purification; Hydrogen-Ion Concentration; Cerium; Lanthanum; Temperature; Kinetics; Water
PubMed: 38844281
DOI: 10.1016/j.ijbiomac.2024.132925 -
Radiographics : a Review Publication of... Jul 2024Osteoid osteoma (OO) is the third most prevalent benign bone neoplasm in children. Although it predominantly affects the diaphysis of long bones, OO can assume an... (Review)
Review
Osteoid osteoma (OO) is the third most prevalent benign bone neoplasm in children. Although it predominantly affects the diaphysis of long bones, OO can assume an intra-articular location in the epiphysis or the intracapsular portions of bones. The most common location of intra-articular OO is the hip joint. The presentation of intra-articular OOs often poses a diagnostic enigma, both from clinical and radiologic perspectives. Initial symptoms are often vague and nonspecific, characterized by joint pain, stiffness, and limited range of motion, which frequently contributes to a delayed diagnosis. Radiographic findings range from normal to a subtle sclerotic focus, which may or may not have a lucent nidus. In contrast to their extra-articular counterparts, intra-articular lesions have distinct features at MRI, including synovitis, joint effusion, and bone marrow edema-like signal intensity. While CT remains the standard for identifying the nidus, even CT may be inadequate in visualizing it in some cases, necessitating the use of bone scintigraphy or fluorine 18-labeled sodium fluoride PET/CT for definitive diagnosis. Radiologists frequently play a pivotal role in suggesting this diagnosis. However, familiarity with the unique imaging attributes of intra-articular OO is key to this endeavor. Awareness of these distinctive imaging findings of intra-articular OO is crucial for avoiding diagnostic delay, ensuring timely intervention, and preventing unnecessary procedures or surgeries resulting from a misdiagnosis. The authors highlight and illustrate the different manifestations of intra-articular OO as compared with the more common extra-articular lesions with respect to clinical presentation and imaging findings. RSNA, 2024 Supplemental material is available for this article.
Topics: Humans; Osteoma, Osteoid; Bone Neoplasms; Diagnosis, Differential; Child; Magnetic Resonance Imaging; Hip Joint; Tomography, X-Ray Computed
PubMed: 38843097
DOI: 10.1148/rg.230208 -
Restorative Dentistry & Endodontics May 2024This study aimed to establish a single-session associative protocol for non-restorative management of dentin hypersensitivity (DH).
OBJECTIVES
This study aimed to establish a single-session associative protocol for non-restorative management of dentin hypersensitivity (DH).
MATERIALS AND METHODS
Twenty-four individuals with DH and a minimum sensitivity level of 4 on the visual analog scale (VAS) were selected. The study was conducted in a split-mouth design, with each participant ( = 20) having at least 1 affected tooth in all quadrants. The management protocols consisted of control group: universal adhesive, Neural Desensitizing Protocol group: 5% potassium nitrate, Mixed Desensitizing Protocol (PAM) group: 5% sodium fluoride and 5% potassium nitrate, Remineralizing Desensitizing Protocol (PDR) group: surface-partially reacted glass technology photopolymerizable varnish. Evaluations were performed immediately after application, at 1 week, 1 month, 2 months, and 12 months using the VAS sensitivity test.
RESULTS
The scores were subjected to statistical analysis using the Friedman test ( < 0.05), Durbin-Conover test ( < 0.05), and Wilcoxon test ( < 0.05). At the 12-month evaluation, all groups showed statistically significant differences compared to the initial assessment. For the evaluation after 12 months, there was a statistically significant difference between the PAM group, the control group, and the PDR group.
CONCLUSIONS
It can be concluded that all groups were effective in controlling DH, but there were significant results in the control group and PDR group. The clinical relevance of this study is to demonstrate that the application of single-session desensitizing protocols can be effective in controlling DH for up to 12 months.
TRIAL REGISTRATION
Brazilian Clinical Trials Registry Identifier: RBR-4r63d7s.
PubMed: 38841389
DOI: 10.5395/rde.2024.49.e15 -
Journal of Oral Biology and... 2024Fluoridated mouth rinses improve anti-cariogenic environment but decrease oral pH below critical value, affecting orthodontic bracket surface topography and causing...
BACKGROUND
Fluoridated mouth rinses improve anti-cariogenic environment but decrease oral pH below critical value, affecting orthodontic bracket surface topography and causing corrosive changes over prolonged use. This invitro study aimed to quantitatively and qualitatively assess the surface topography and metallic ion release of the stainless steel (SS) brackets at varying acidic and alkaline pH.
MATERIALS AND METHODS
Forty unused SS brackets were divided into four groups (Group A, B, C, D) and immersed for 48- hours in solutions of artificial saliva and sodium fluoride (0.2 %) mouth rinse at varying pH of 5.5,6.7,7 and 8. The surface morphologic changes were analyzed under scanning electron microscope (SEM) at 50×, 150×, and 500× magnification. The changes in slot area were scored using the customized scale. The Energy Dispersive Xray Spectroscopy Analysis (EDAX) was used to estimate the probed elements' atomic and weight percentage.
RESULTS
The mean score of the scale was 3.4 for the brackets immersed in the acidic solution which was statistically significant (p = 0.00)and for alkaline and neutral solutions (p = 0.00). Chromium was found to be significantly higher in the alkaline solution (p = 0.016) followed by the neutral solution. Carbon was found excess in acidic solution than the neutral and alkaline solution.
CONCLUSION
Quantitative and qualitative analysis of the ion release in stainless steel brackets using SEM and EDAX revealed the corrosive effect of fluoride ion causing maximum surface changes in acidic medium and chromium release in alkaline pH.
PubMed: 38832295
DOI: 10.1016/j.jobcr.2024.05.001 -
Heliyon May 2024The ability of ureolytic bacteria to break down stable urea to alkaline ammonia leads to several environmental and health challenges. Ureolytic bacteria such as and...
Synergistic inhibition of ureolytic activity and growth of suggests cobinding of fluoride and acetohydroxamic acid at the urease active site and provides a novel strategy to combat ureolytic bacteria.
The ability of ureolytic bacteria to break down stable urea to alkaline ammonia leads to several environmental and health challenges. Ureolytic bacteria such as and can become pathogenic and cause persistent infections that can be difficult to treat. Inhibiting urease activity can reduce the growth and pathogenicity of ureolytic bacteria. In the present study, we investigated the synergistic effects of tannic acid (TA) and the urease inhibitors fluoride (F) and acetohydroxamic acid (AHA). The concentration of AHA needed for efficient inhibition of the ureolytic activity of can be significantly reduced if AHA is coapplied with tannic acid and sodium fluoride (NaF). Thus, only 1.20 μmol l AHA in combination with 0.30 mmol l tannic acid and 0.60 mmol l NaF delayed the onset of ureolytic pH increase by 95.8 % and increased the growth lag phase by 124.3 % relative to untreated . At these concentrations, without AHA, TA and NaF increased the onset of the ureolytic pH change by only 37.0 % and the growth lag phase by 52.5 %. The strong inhibition obtained with low concentrations of AHA in triple-compound treatments suggests cobinding of F and AHA at the urease active site and could reduce the side effects of AHA when it is employed as a drug against e.g. urinary tract infections (UTIs) and blocked catheters. This study reports the basis for a promising novel therapeutic strategy to combat infections caused by ureolytic bacteria and the formation of urinary tract stones and crystalline biofilms on catheters.
PubMed: 38826744
DOI: 10.1016/j.heliyon.2024.e31209