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Journal of Conservative Dentistry and... May 2024The aim of the study is to check the antibacterial efficacy of various ayurvedic oils used as a solvent with zinc oxide for preparing endodontic sealers.
AIM
The aim of the study is to check the antibacterial efficacy of various ayurvedic oils used as a solvent with zinc oxide for preparing endodontic sealers.
MATERIALS AND METHODS
Forty-five extracted premolars were taken and were cut coronally and apically such that 7 mm of tooth specimen was prepared. Teeth were sterilized by autoclaving inoculated with and incubated for 24 h. The specimens were divided into three groups of 15 each. Group 1 - ZnO powder + Eugenol, Group 2 - ZnO powder + Aremidadi Oil, and Group 3 - ZnO powder + Dashmool oil. Bacterial growth in each specimen was calculated before and after sealer application and noted as the initial and final colony count. The antimicrobial effect of each sealer was measured by calculating the percentage reduction in colony count (%). One-way analysis of variance and tests will be used for statistical analysis.
RESULTS
The Zn + Arimedadi oil group showed the maximum antibacterial effect among the sealers tested and the Zn + eugenol sealer showed the least antimicrobial effect In comparison, there was a statistically significant difference between all the groups.
CONCLUSION
Ayurvedic oil-based root canal sealers showed better antibacterial efficacy than eugenol-based sealers. Arimedadi oil showed the highest antibacterial activity against and Eugenol showed the least when used as a solvent.
PubMed: 38939540
DOI: 10.4103/JCDE.JCDE_119_24 -
Journal of Conservative Dentistry and... May 2024The purpose of this study is to comparatively evaluate the effect of discoloration of nanohybrid composite by four different phytopigments.
OBJECTIVE
The purpose of this study is to comparatively evaluate the effect of discoloration of nanohybrid composite by four different phytopigments.
MATERIALS AND METHODS
Fifty disk-shaped samples of nanohybrid (3M Filtek Z350) resin composites were prepared using an acrylic template of dimension 5 mm × 3 mm. They were randomly divided into five groups and immersed in solutions of tomato powder, beetroot powder, java plum powder, and turmeric powder. Distilled water was used as the control group. The samples were placed in respective solutions for 3 h daily and stored in artificial saliva for the rest of the day for 28 days. Color values (L*, a*, b*) were measured by colorimeter using the CIE L*a*b* system at the end of the 7 and 28 days of immersion. Color differences ΔE*ab were statistically analyzed.
RESULTS
All the samples showed a change in color of nanohybrid composite resin to varying degrees. The mean ΔE*ab value obtained with beetroot solution was the highest among all the groups at the end of the 7 and 28 days, depicting that beetroot solution showed maximum mean color variation, followed by java plum solution, turmeric solution, and tomato solution.
CONCLUSION
All the phytopigments used in this study have the potential to discolor the nanohybrid composite resin, with beetroot causing the most severe discoloration.
PubMed: 38939539
DOI: 10.4103/JCDE.JCDE_169_24 -
Journal of Conservative Dentistry and... May 2024To evaluate cleaning efficacy and debris extrusion of supplementary file systems XP Endo Finisher (XPEF) and XP Endo Finisher R (XPEFR) in endodontic retreatment.
AIM
To evaluate cleaning efficacy and debris extrusion of supplementary file systems XP Endo Finisher (XPEF) and XP Endo Finisher R (XPEFR) in endodontic retreatment.
MATERIALS AND METHODS
Thirty single-rooted teeth with single canals were selected, canal preparation done till file #30 6% and obturation completed using lateral condensation technique with AH Plus sealer. The samples were stored at 37°C in a 100% humidity incubator for 7 days. The samples were distributed across the three groups according to the method of retreatment ( = 10): Group I: Neo Endo retreatment (NER) files, Group II: NER files + XPEF, and Group III: NER files + XPEFR. Removal of gutta percha using each file system according to the distributed groups was performed. The extruded debris was collected in an Eppendorf tube, dried in a hot air oven, and weighed. Teeth were sliced longitudinally using carborundum discs. Coronal, middle, and apical thirds were assessed for cleaning efficacy under a stereomicroscope. Results were tabulated and subjected to the statistical analysis using the Kruskal-Wallis -test followed by turkey HSD test. All statistical tests were carried out at significance level < 0.05.
RESULTS
It was seen that Group II (NER files + XPEF) exhibited better cleaning efficacy than Group III (NER files + XPEFR), although the results were not statistically significant. Greater debris extrusion was seen with Group III when compared to Group II.
CONCLUSION
Supplementary files XPEF/XPEFR enhance the cleaning efficacy in endodontic retreatment, but the debris extrusion of XPEFR is more than XPEF.
PubMed: 38939537
DOI: 10.4103/JCDE.JCDE_90_24 -
Journal of Conservative Dentistry and... May 2024This study aims to evaluate the microleakage between the gingival seat and base material and to assess the interface integrity between the base material and overlying...
Deep margin elevation in class II cavities: A comparative evaluation of microleakage and interface integrity using confocal laser microscopy and scanning electron microscopy.
AIM
This study aims to evaluate the microleakage between the gingival seat and base material and to assess the interface integrity between the base material and overlying composite in class II cavities restored using deep margin elevation.
MATERIALS AND METHODS
Thirty maxillary molars ( = 30) were taken, and class II cavities were prepared with a gingival seat extending below the cementoenamel junction. These teeth were divided into three groups for subgingival margin elevation using different materials: Group A ( = 10) - flowable composite, Group B ( = 10) - glass ionomer cement (GIC), and Group C ( = 10) - GIC with nanohydroxyapatite (GIC n-HAp). The remaining cavities were restored with bulk-fill composite. After undergoing 1000 thermocycling cycles, half of the samples were examined for microleakage using confocal laser microscopy, and the other half were assessed for interface integrity using scanning electron microscopy. Microleakage was statistically analyzed by one-way ANOVA, and interface integrity was analyzed by Kruskal-Wallis tests.
RESULTS
The study found that GIC n-HAp exhibited significantly lower microleakage between the base material and gingival seat than flowable composite and GIC. However, regarding interface integrity between the base material and bulk-fill composite, flowable composite, and GIC outperformed GIC n-HAp.
CONCLUSIONS
Incorporating n-HAp into GIC effectively reduced microleakage at the dentin-base material interface. However, the interface integrity between GIC n-HAp and the composite poses a challenge.
PubMed: 38939536
DOI: 10.4103/JCDE.JCDE_155_24 -
Journal of Conservative Dentistry and... May 2024To evaluate the efficacy of incorporated novel additives in Glass Ionomer Cement to ameliorate biocompatibility and mechanical properties.
AIM
To evaluate the efficacy of incorporated novel additives in Glass Ionomer Cement to ameliorate biocompatibility and mechanical properties.
INTRODUCTION
Though Glass Ionomer Cement (GIC) has multiple advantages, it is not strong enough for medical applications, and its biocompatibility is questionable. To improve biocompatibility and its mechanical properties, a study was performed to investigate the potential benefits of adding graphene, carbon nanotubes, hydroxyapatite, and bioactive glass to GIC. The objective was to enhance both the mechanical properties and biocompatibility of GIC.
MATERIAL AND METHOD
Modified Glass Ionomer Cement was prepared by creating five groups. Hydroxyapatite, multi-walled carbon nanotubes, graphene, and bioactive glass were incorporated in a 10:1 weight ratio, respectively. Group 5 was designated as the control group and used Fuji Type II GIC. After preparing 90 samples, they were kept in deionized water for a day and then evaluated their compressive strength, microhardness, and diametral tensile strength, and also checked their in vitro cytotoxicity by direct contact with L929 mammalian fibroblast cells.
STATISTICAL ANALYSIS
The data were examined using mean and standard deviation descriptive statistics. The comparative evaluation was done via Tukey HSD test and one-way ANOVA using S.P.S.S. software.
RESULT
It showed that Group 3 had better results in compressive strength (144.478+- 3.989), diametral tensile strength (20.29+- 0.8601), and microhardness (131+-3.536) when compared with other groups while in the biocompatibility (viability %) Group 1 [82.55], Group 3 [76.49], Group 4 [87.63], while Group 2[58.02].
CONCLUSION
Group 3 has better physical properties in microhardness, diametral tensile strength, and compressive strength, than the other groups. In Biocompatibility, Group 1, Group 3, Group 4, and Group 5 were noncytotoxic at the same time multi-walled carbon nanotubes group (Group 2) had cytotoxic potential.
PubMed: 38939535
DOI: 10.4103/JCDE.JCDE_81_24 -
Cureus May 2024Root resorption is a challenging endodontic case in terms of the management of both hard and soft tissues in patients. It requires thorough knowledge, the proper choice...
Root resorption is a challenging endodontic case in terms of the management of both hard and soft tissues in patients. It requires thorough knowledge, the proper choice of material, and regular follow-ups. Several etiological factors are responsible for the susceptibility of the tooth to resorption. The most common are dental caries and trauma. This case report sheds light on the etiopathogenesis of the development of internal root resorption and the clinical management of the resorptive defect. It also focuses on the need for proper diagnostic methodology for treating such complex defects.
PubMed: 38939253
DOI: 10.7759/cureus.61214 -
Cureus May 2024This is a unique case of a single-rooted maxillary premolar with two separate canals in bayonet or S-shaped radicular anatomy undergoing post-endodontic reinforcement...
This is a unique case of a single-rooted maxillary premolar with two separate canals in bayonet or S-shaped radicular anatomy undergoing post-endodontic reinforcement prior to crown placement. Bayonet-shaped canals present challenges in canal negotiation, cleaning, shaping, and obturation. The complexity of these canals heightens the risk of accidental file breakage and other iatrogenic errors, posing significant challenges. Post-endodontic restoration in S-shaped canals with double curvature poses challenges in precise placement and material adaptation. A novel technique was implicated to preserve the functionality and structural aesthetics of a decayed maxillary first premolar tooth.
PubMed: 38939248
DOI: 10.7759/cureus.61224 -
Cureus May 2024is a new genus of anaerobic bacteria discovered in the year 2000. It is one of the most common bacteria present in the host microbial flora of dental plaque. The levels...
Comparative Evaluation of Mogibacterium timidum in the Subgingival Plaque of Periodontally Healthy and Chronic Periodontitis Patients: A Real-Time Polymerase Chain Reaction (PCR) Study.
BACKGROUND
is a new genus of anaerobic bacteria discovered in the year 2000. It is one of the most common bacteria present in the host microbial flora of dental plaque. The levels of are supposedly higher in inflammatory conditions.
AIMS AND OBJECTIVES
This study aimed to quantify the levels of species in the subgingival plaque samples of healthy patients and patients with chronic periodontitis.
MATERIALS AND METHODS
A total of 24 samples of the subgingival plaque, 12 healthy samples and 12 samples of chronic periodontitis patients, were collected in a buffer solution using a sterile Gracey curette. These samples were then sent to a laboratory for real-time polymerase chain reaction (PCR) testing.
RESULTS
was found in higher quantities in plaque samples taken from chronic periodontitis patients when compared to healthy patients.
CONCLUSION
can be said to be associated with chronic periodontitis condition. Further studies are required to know the exact nature of the pathogen.
PubMed: 38939247
DOI: 10.7759/cureus.61211 -
International Journal of Dentistry 2024To determine the minimum dentin thickness in the mesial and distal walls of the mesiobuccal (MB) and mesiolingual (ML) canals of the mandibular first molars using...
AIM
To determine the minimum dentin thickness in the mesial and distal walls of the mesiobuccal (MB) and mesiolingual (ML) canals of the mandibular first molars using cone-beam computed tomography (CBCT).
MATERIALS AND METHODS
CBCT examinations of 624 mandibular first molars from an Indian subpopulation were analyzed. The mesial and distal minimum dentin thickness was evaluated in 1 mm intervals apical to the furcation area. Independent -test was used to analyze the data ( = 0.05). Using Cohen's kappa coefficient, the interexaminer and intraexaminer reliability was evaluated.
RESULTS
The mesial dentin thickness was significantly higher than the distal dentin thickness for MB and ML canals (=0.01). The average dentin thickness in the distal and mesial plane of the MB canal was 1.15 ± 0.15 mm and 1.52 ± 0.19 mm at the 1 mm level and 0.83 ± 0.13 and 1.08 ± 0.18 at the 5 mm level, respectively. For the ML canal, the average dentin thickness in the distal plane and the mesial plane was 1.24 ± 0.18 mm and 1.44 ± 0.21 at the 1 mm level and 0.91 ± 0.16 and 1.01 ± 0.17 at the 5 mm level, respectively. Statistical analysis between the MB and ML canals showed significant differences in the dentin thickness at 4 and 5 mm levels in both the distal and the mesial planes (=0.01). In more than 85% of the cases, the minimum dentin thickness was seen at the 5 mm level in both the distal and mesial planes in MB and ML canals.
CONCLUSION
The distal planes of the mesiolingual and mesiobuccal canals were thinner in most cases, making the distal surface more prone to iatrogenic perforations. Considerably, at 4 and 5 mm from the furcation, the distal wall was significantly thinner than the mesial walls. Understanding the anatomy of the danger zone in the mesial roots of the mandibular first molars may serve to minimize the risk of endodontic mishaps such as strip perforations.
PubMed: 38938693
DOI: 10.1155/2024/8823070 -
Journal of Nanobiotechnology Jun 2024Efficient monitoring of glucose concentration in the human body necessitates the utilization of electrochemically active sensing materials in nonenzymatic glucose...
Controlled synthesized of ternary Cu-Co-Ni-S sulfides nanoporous network structure on carbon fiber paper: a superior catalytic electrode for highly-sensitive glucose sensing.
BACKGROUND
Efficient monitoring of glucose concentration in the human body necessitates the utilization of electrochemically active sensing materials in nonenzymatic glucose sensors. However, prevailing limitations such as intricate fabrication processes, lower sensitivity, and instability impede their practical application. Herein, ternary Cu-Co-Ni-S sulfides nanoporous network structure was synthesized on carbon fiber paper (CP) by an ultrafast, facile, and controllable technique through on-step cyclic voltammetry, serving as a superior self-supporting catalytic electrode for the high-performance glucose sensor.
RESULTS
The direct growth of free-standing Cu-Co-Ni-S on the interconnected three-dimensional (3D) network of CP boosted the active site of the composites, improved ion diffusion kinetics, and significantly promoted the electron transfer rate. The multiple oxidation states and synergistic effects among Co, Ni, Cu, and S further promoted glucose electrooxidation. The well-architected Cu-Co-Ni-S/CP presented exceptional electrocatalytic properties for glucose with satisfied linearity of a broad range from 0.3 to 16,000 μM and high sensitivity of 6829 μA mM cm. Furthermore, the novel sensor demonstrated excellent selectivity and storage stability, which could successfully evaluate the glucose levels in human serum. Notably, the novel Cu-Co-Ni-S/CP showed favorable biocompatibility, proving its potential for in vivo glucose monitoring.
CONCLUSION
The proposed 3D hierarchical morphology self-supported electrode sensor, which demonstrates appealing analysis behavior for glucose electrooxidation, holds great promise for the next generation of high-performance glucose sensors.
Topics: Copper; Electrodes; Nickel; Catalysis; Humans; Cobalt; Electrochemical Techniques; Biosensing Techniques; Sulfides; Carbon Fiber; Glucose; Nanopores; Oxidation-Reduction; Blood Glucose
PubMed: 38937768
DOI: 10.1186/s12951-024-02635-w