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International Journal of General... 2023Low volume change and minimal trauma observed during angiography are the reason why physicians often overlook any changes affecting pre-operative electrolytes levels...
BACKGROUND
Low volume change and minimal trauma observed during angiography are the reason why physicians often overlook any changes affecting pre-operative electrolytes levels after coronary intervention. However, few studies have addressed the issue of electrolyte changes after the coronary intervention. Therefore, our study investigates coronary angiography's effect on electrolytes and provides the quick identification of groups more prone to electrolyte changes.
METHODS
From the department of cardiology of the second affiliated hospital of Shandong's first medical university, 374 patients undergoing coronary angiography were selected. Pre-intervention and post-intervention serums, sodium (Na), potassium (K), chloride (Cl), magnesium (Mg) and renal function were analyzed. The correlation between influential factors was also assessed. The association of hypokalemia with short-major adverse cardiac events (MACE) and arrhythmia was evaluated.
RESULTS
Among the 374 subjects including 264 patients who had a simple angiography and 110 patients who received coronary artery interventional therapy. A decrease in potassium levels was found in 81.8% of the patients, and post-interventional hypokalemia was observed in 15.0%. After the intervention, the hypokalemia among males was 2.18 times than that of females, and the pre-operative serum potassium level was 3.5mmol/L≤K<4.0mmol/L and was 2.09 times than that of K≥4.0 mmol/L, but was not associated with age and either simple coronary angiography or PCI (percutaneous coronary intervention). Hypernatremia was also prevalent in males under 60 years and with pre-operative hypernatremia. Significant variations were found between hypokalemia and influential factors like hypertension, diabetes, and gastrointestinal disease. We also found that there was no obvious correlation between hypokalemia and recurrent angina, heart failure and death, but significantly increased the risk of some arrhythmias.
CONCLUSION
Male patients are more likely to suffer from electrolyte disturbance after coronary intervention. There is a need to emphasize monitoring and managing electrolyte changes to prevent severe complications in the peri-operative period.
PubMed: 37868813
DOI: 10.2147/IJGM.S430510 -
Molecular Therapy. Methods & Clinical... Dec 2023Recombinant adeno-associated viral vector (rAAV) mediated gene therapy is gaining traction in treating genetic disorders. Current rAAV production systems yield a mixture...
Recombinant adeno-associated viral vector (rAAV) mediated gene therapy is gaining traction in treating genetic disorders. Current rAAV production systems yield a mixture of capsids largely devoid of the transgene (empty capsid) compared with the desired therapeutic product (full capsid). Anion exchange chromatography (AEX) is an attractive method for separating empty and full AAV capsids because of its scalability. Resin types and buffer composition are key considerations for AEX and must support capsid stability to be suitable for downstream processing. We examined the impact of binding durations (0-8 h) using various binding ionic strengths (15-75 mM), pH (7.5-9.0), resin chemistry (POROS XQ, POROS HQ, POROS I, and BIA QA monolith), and proprietary Q resins with different ligand densities for effects on capsid stability. Empty capsids were altered upon extended binding, leading to retention time shifts and loss of resolution between empty and full capsids. Viral capsid protein analysis reveals that full capsids have more viral capsid protein 3 (VP3) proteins than empty capsids. Analytical hydrophilic liquid chromatography showed that empty capsid retention time shift is accompanied by changes to the empty capsid's native VP3 protein. Among the potential stabilizing additives considered, magnesium chloride was the most effective at reducing negative impacts caused by extended binding.
PubMed: 37868210
DOI: 10.1016/j.omtm.2023.101112 -
Frontiers in Veterinary Science 2023This study aimed to establish baseline variables for calves transported by road and ferry from Ireland to the Netherlands and to investigate the effect of journey [two...
This study aimed to establish baseline variables for calves transported by road and ferry from Ireland to the Netherlands and to investigate the effect of journey [two comparable journeys in April (J1) and May (J2) 2022] and source [source farm or mart (SF/MA)] on these variables. A total of 66 calves from the SF/MA were transported from Ireland to commercial veal farms in the Netherlands. Blood samples were collected at the SF/MA, assembly center (Ireland), lairage (France), and on arrival on the veal farm (Netherlands). They were analyzed for indicator variables related to energy balance, hydration/electrolytes, physical/muscular stress, immunity, and inflammation [glucose, beta-hydroxybutyrate (BHB), non-esterified fatty acids (NEFA), potassium, sodium, magnesium, chloride, urea, haematocrit, total protein, creatine kinase, L-lactate, cortisol, white blood cell, neutrophil, lymphocyte and monocyte counts, serum amyloid-A, and haptoglobin]. Health variables eye and nose discharge, skin tent (a measure of dehydration), and navel inflammation were scored by a trained observer, and calves were weighed at every blood-sampling time point. All blood variables and body weight changed significantly ( < 0.05) during transport, most notably between the assembly center and lairage. Reference ranges were available for 18 variables; 11 of these variables exceeded the reference ranges at the lairage, whilst 10 variables exceeded the reference ranges on arrival at the veal farm. However, health variables did not change during transport. A journey-to-journey comparison indicated much variation; 18 out of 25 variables differed significantly on at least one time point. In total, J1 calves experienced a more severe change in BHB, potassium, strong-ion-difference, L-lactate, and eye and nose discharge than J2 calves. The source of calves also affected their physiology; 12 out of 25 variables studied differed significantly, all of which were confined to the first time point. Specifically, MA calves had elevated levels of NEFA, urea, haematocrit, L-lactate, cortisol, white blood cell, neutrophil, and monocyte counts and lower levels of corrected chloride and lymphocyte count. Overall, calves in this study showed a generalized physiological disturbance beyond reference limits during long-distance transport, but no animal died during transport or for 3 weeks post-arrival.
PubMed: 37829357
DOI: 10.3389/fvets.2023.1238734 -
Heliyon Sep 2023Groundwater resources around the world required periodic monitoring in order to ensure the safe and sustainable utilization for humans by keeping the good status of...
Groundwater resources around the world required periodic monitoring in order to ensure the safe and sustainable utilization for humans by keeping the good status of water quality. However, this could be a daunting task for developing countries due to the insufficient data in spatiotemporal resolution. Therefore, this research work aimed to assess groundwater quality in terms of drinking and irrigation purposes at the adjacent part of the Rooppur Nuclear Power Plant (RNPP) in Bangladesh. For the purposes of achieving the aim of this study, nine groundwater samples were collected seasonally (dry and wet season) and seventeen hydro-geochemical indicators were analyzed, including Temperature (Temp.), pH, electrical conductivity (EC), total dissolved solids (TDS), total alkalinity (TA), total hardness (TH), total organic carbon (TOC), bicarbonate (HCO), chloride (Cl), phosphate (PO), sulfate (SO), nitrite (NO), nitrate (NO), sodium (Na), potassium (K), calcium (Ca) and magnesium (Mg). The present study utilized the Canadian Council of Ministers of the Environment water quality index (CCME-WQI) model to assess water quality for drinking purposes. In addition, nine indices including EC, TDS, TH, sodium adsorption ratio (SAR), percent sodium (Na%), permeability index (PI), Kelley's ratio (KR), magnesium hazard ratio (MHR), soluble sodium percentage (SSP), and Residual sodium carbonate (RSC) were used in this research for assessing the water quality for irrigation purposes. The computed mean CCME-WQI score found higher during the dry season (ranges 48 to 74) than the wet season (ranges 40 to 65). Moreover, CCME-WQI model ranked groundwater quality between the "poor" and "marginal" categories during the wet season implying unsuitable water for human consumption. Like CCME-WQI model, majority of the irrigation index also demonstrated suitable water for crop cultivation during dry season. The findings of this research indicate that it requires additional care to improve the monitoring programme for protecting groundwater quality in the RNPP area. Insightful information from this study might be useful as baseline for national strategic planners in order to protect groundwater resources during the any emergencies associated with RNPP.
PubMed: 37809741
DOI: 10.1016/j.heliyon.2023.e19668 -
PloS One 2023The aim of this study is to evaluate the synergistic effect of polyester fiber-reinforced and nanoslica on the technical performance and durability of geopolymer mortar...
The aim of this study is to evaluate the synergistic effect of polyester fiber-reinforced and nanoslica on the technical performance and durability of geopolymer mortar in terms of the chemical resistance. The study examined how the addition of polyester fiber and nanosilica affects the short-term severe durability of geopolymer mortar specimens made with fly ash (type F). The specimens were cured under ambient conditions. Different percentages (0.6%, 1.2%, and 1.8%) of polyester fiber were used, both with and without nanosilica. Additionally, a reference mixture containing only nanosilica was prepared.All mixtures had a liquid to binder ratio of 0.50, and the ratio of NaOH to Na2SiO3 solution was kept at 2.5:1 by weight. The produced mixes, after 28 days of ambient curing, were immersed for another 28 days in solutions containing 3.5%, 5%, and 5% of sodium chloride, magnesium sulphate and sulfuric acid, respectively. For comparison, control specimens which were not exposed to chemical attacks were tested at the same age of 56 days. Moreover, water absorption and sorptivity tests were conducted to explain the durability performance in a more detailed way. The test results express that the combination of both materials showed a synergistic effect and resulted in greater improvements in compressive and flexural strengths. Both materials can reduce the reduction in compressive strength caused by sulfuric acid exposure, but polyester fiber can increase mass loss. The presence of magnesium sulfate and sodium chloride can lead to a reduction in strength, but the addition of both polyester fiber and nanosilica can mitigate these effects. The addition of fibers creates a network of pores that can limit water absorption, and nanosilica can further enhance the microstructure and reduce water absorption. However, using polyester fiber beyond 1.2 percent can adversely affect the rate of water absorption.
Topics: Sulfuric Acids; Coal Ash; Compressive Strength; Magnesium Sulfate; Polyesters; Silicon Dioxide; Sodium Chloride
PubMed: 37751445
DOI: 10.1371/journal.pone.0289497 -
Applied Microbiology and Biotechnology Dec 2023Vibrio natriegens is a halophilic bacterium with the fastest generation time of non-pathogenic bacteria reported so far. It therefore has high potential as a production...
Vibrio natriegens is a halophilic bacterium with the fastest generation time of non-pathogenic bacteria reported so far. It therefore has high potential as a production strain for biotechnological production processes or other applications in biotechnology. Culture media for V. natriegens typically contain high sodium chloride concentrations. The corresponding high chloride concentrations can lead to corrosion processes on metal surfaces in bioreactors. Here we report the development of a low-chloride chemically defined medium for V. natriegens. Sodium chloride was completely replaced by the sodium salts disodium hydrogen phosphate, disodium sulfate, and sodium citrate, while keeping the total concentration of sodium ions constant. The use of citrate prevents the occurrence of precipitates, especially of ammonium magnesium phosphate. With this defined medium, high-cell-density fed-batch cultivations in laboratory-scale bioreactors using exponential feeding yielded biomass concentrations of more than 60 g L. KEY POINTS: A defined medium for V. natriegens that only contains traces of chloride was developed Corrosion processes on metal surfaces in industrial bioreactors can thus be prevented High yields of biomass can be achieved in fed-batch cultivation with this medium.
Topics: Chlorides; Sodium Chloride; Bioreactors; Vibrio; Sodium
PubMed: 37741940
DOI: 10.1007/s00253-023-12799-4 -
Crystal Growth & Design Sep 2023Magnesium hydroxide, Mg(OH), is an inorganic compound extensively employed in several industrial sectors. Nowadays, it is mostly produced from magnesium-rich minerals....
Magnesium hydroxide, Mg(OH), is an inorganic compound extensively employed in several industrial sectors. Nowadays, it is mostly produced from magnesium-rich minerals. Nevertheless, magnesium-rich solutions, such as natural and industrial brines, could prove to be a great treasure. In this work, synthetic magnesium chloride and sodium hydroxide (NaOH) solutions were used to recover Mg(OH) by reactive crystallization. A detailed experimental campaign was conducted aiming at producing grown Mg(OH) hexagonal platelets. Experiments were carried out in a stirred tank crystallizer operated in single- and double-feed configurations. In the single-feed configuration, globular and nanoflakes primary particles were obtained, as always reported in the literature when NaOH is used as a precipitant. However, these products are not complying with flame-retardant applications that require large hexagonal Mg(OH) platelets. This work suggests an effective precipitation strategy to favor crystal growth while, at the same time, limiting the nucleation mechanism. The double-feed configuration allowed the synthesis of grown Mg(OH) hexagonal platelets. The influence of reactant flow rates, reactant concentrations, and reaction temperature was analyzed. Scanning electron microscopy (SEM) pictures were also taken to investigate the morphology of Mg(OH) crystals. The proposed precipitation strategy paves the road to satisfy flame-retardant market requirements.
PubMed: 37692336
DOI: 10.1021/acs.cgd.3c00462 -
Medicine Sep 2023The diagnosis of Gentleman syndrome (GS) is usually delayed because the clinical symptoms are easily mistaken.
RATIONALE
The diagnosis of Gentleman syndrome (GS) is usually delayed because the clinical symptoms are easily mistaken.
PATIENT CONCERNS
A 19-year-old male patient was referred to endocrinology due to intermittent twitch of extremities for approximately 7 years.
DIAGNOSES
The diagnosis of GS was made based on the laboratory and gene detection results. We identified 2 new variants in the SLC12A3 gene [c.857 A > C (exon7) and c.2089_2095del (exon17)] in his Asian family.
INTERVENTIONS
The patient received the treatment of potassium chloride sustained release tablets, potassium magnesium aspartate and spironolactone. After given potassium supplement through enema, his serum potassium level was corrected to normal.
OUTCOMES
The electrolyte imbalance including hypokalemia and hypomagnesemia were improved with a remission of the clinical manifestations. But the patient's condition still could not remain stable for his irregular oral potassium supplementation during the follow-up of nearly 3 months.
LESSONS
Our finding broadens the variant spectrum of SLC12A3 and contributes to a more quickly genetic counseling. As a result, when a patient presents with persistent, unspecified, and inadequately treated hypokalemia, tests for GS should indeed be considered. For suspected cases of GS, genetic testing should always be considered in the diagnosis.
Topics: Male; Humans; Young Adult; Adult; Gitelman Syndrome; Hypokalemia; Pedigree; East Asian People; Mutation; Solute Carrier Family 12, Member 3
PubMed: 37657006
DOI: 10.1097/MD.0000000000034967 -
Frontiers in Veterinary Science 2023Uroliths are concretions formed in the urinary tract. These can be problematic in humans and companion animals such as cats. Magnesium ammonium phosphate (struvite) and...
INTRODUCTION
Uroliths are concretions formed in the urinary tract. These can be problematic in humans and companion animals such as cats. Magnesium ammonium phosphate (struvite) and calcium oxalate (CaOx) are the most common forms of uroliths. The relative supersaturation (RSS) is a relative risk index of crystal formation. Here, an updated program for calculating RSS, EQUIL-HL21, was used to detect differences in RSS values when cats were fed foods formulated for urinary and non-urinary conditions. In addition, the contributions of urinary analytes to RSS values were examined via regression analyses.
METHODS
Historical data from feeding trials including foods indicated for use in urinary or non-urinary conditions were analyzed for nutrient composition and urinary parameters. RSS was calculated by EQUIL-HL21. The relationship between RSS values calculated by EQUIL-HL21 and urinary analytes was examined by regression models, which were selected by R and stepwise methods.
RESULTS
Cats that consumed urinary foods had significantly greater levels of urinary sodium and chloride compared with those that consumed non-urinary foods, consistent with the greater amounts of sodium and chloride in the urinary foods. Those that consumed non-urinary foods had higher urine pH, ammonium, potassium, phosphorus, magnesium, oxalate, citrate, and sulfate. Struvite RSS value and number of urinary crystals were significantly lower in cats fed the urinary foods. Mean CaOx RSS values were similar in both foods, though the number of CaOx crystals were significantly higher in cats that consumed non-urinary foods. A model predicting the natural log of struvite RSS values indicated that these values would increase with increasing urine pH, ammonium, chloride, calcium, phosphorus, and magnesium, and would decrease with increasing urine citrate and sulfate. CaOx RSS was predicted to increase as urinary chloride, calcium, and oxalates increased, and would decrease as urine pH, sodium, phosphorus, citrate, and sulfate increased.
DISCUSSION
These analyses demonstrate that the EQUIL-HL21 program can accurately detect expected differences between foods formulated for urinary and non-urinary indications. Regression models showed the eight urinary analytes that, respectively, contribute to the predicted RSS values for struvite and CaOx.
PubMed: 37601750
DOI: 10.3389/fvets.2023.1167840 -
Molecules (Basel, Switzerland) Aug 2023Surface impregnation of concrete structures with a migrating corrosion inhibitor is a promising and non-invasive technique for increasing the lifetime of existing...
Surface impregnation of concrete structures with a migrating corrosion inhibitor is a promising and non-invasive technique for increasing the lifetime of existing structures that already show signs of corrosion attack. The main requirement for inhibitors is their ability to diffuse the rebar at a sufficient rate to protect steel. The use of smart nanocontainers such as layered double hydroxides (LDH) to store corrosion inhibitors significantly increases efficiency by providing an active protection from chloride-induced corrosion. The addition of LDH to reinforced mortar can also improve the compactness and mechanical properties of this matrix. Here, we report the synthesis of a magnesium-aluminum LDH storing glutamine amino acid as a green inhibitor (labeled as Mg-Al-Gln), which can be used as a migrating inhibitor on mortar specimens. The corrosion behavior of the specimens was determined via electrochemical techniques based on measurements of corrosion potential and electrochemical impedance spectroscopy. A cell containing a 3.5% NaCl solution was applied to the mortar surface to promote the corrosion of embedded rebars. The specimens treated with Mg-Al-Gln presented an improved corrosion protection performance, exhibiting an increase in polarization resistance (Rp) compared to the reference specimens without an inhibitor (NO INH). This effect is a consequence of a double mechanism of protection/stimuli-responsive release of glutamine and the removal of corrosive chloride species from the medium.
PubMed: 37570833
DOI: 10.3390/molecules28155863