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Saudi Medical Journal Jan 2023To update a possible role of cosmeceutical topic treatment to obtain a better scar.
OBJECTIVES
To update a possible role of cosmeceutical topic treatment to obtain a better scar.
METHODS
This is a preliminary supportive study. A total of 14 patients who went to the General Hospital of Mexico City, Mexico, between May and December 2020, for breast reconstruction were included in the current study. The biopsies were carried out to the scar area of the previous I° and II° surgery. The patients were thus divided into 2 groups: those who used Cicolea cream® as a treatment supplement and those who used only petrolatum.
RESULTS
Collagen fibers arranged in a regular pattern in the group treated with Cicolea compared to dispersed collagen fibers in the group treated with pure petrolatum. Furthermore, the patients who presented hypertrophic or keloid scars secondary to mastectomy, developed after insertion of breast expanders an organized scarring process, with improvement of scar if treated with Cicolea.
CONCLUSION
Based on our observations, it is possible to propose that the action of the polyphenols present in the different components of Cicolea® cream leads to a better evolution of the wound healing compared to the action of petrolatum composition.
Topics: Humans; Female; Breast Neoplasms; Cicatrix, Hypertrophic; Mastectomy; Wound Healing; Skin; Petrolatum; Collagen
PubMed: 36634956
DOI: 10.15537/smj.2023.44.1.20220556 -
Human & Experimental Toxicology 2023Aluminum phosphide (AlP) poisoning is a serious medical emergency with a high mortality rate. The absence of an exact antidote for AlP poisoning necessitates the quest... (Randomized Controlled Trial)
Randomized Controlled Trial
Comparing the effectiveness of L-carnitine and paraffin oil in acute aluminum phosphide poisoning using predictive biomarkers and scores: A randomized controlled clinical trial.
Aluminum phosphide (AlP) poisoning is a serious medical emergency with a high mortality rate. The absence of an exact antidote for AlP poisoning necessitates the quest for alternative treatment options. The study sought to assess the efficacy of adding L-carnitine or medicated paraffin oil to the conventional approach of treatment employed in cases of acute AlP poisoning. We conducted a 1 year, randomized, controlled, parallel-group, single-blind clinical study. 96 individuals with acute AlP poisoning were randomly assigned to one of three groups. The standard AlP therapy was administered to all groups according to the Poison Control Center guidelines at the Ain-Shams University hospitals. All patients underwent a medical history review, clinical examination, and laboratory tests. The outcomes were assessed. The participants in the study groups had mean ages ranging from 25.6 to 26.3 years. The cases analyzed were evenly distributed between genders, with the majority originating from rural areas. The average delay time varied from 2.9 to 4.2 h. All patients in the study reported ingesting AlP during suicide attempts. 12 hours after admission, many clinical and biochemical data improved in both intervention groups including cytochrome c oxidase, caspase-3, caspase-9, catalase, and superoxide dismutase. The intervention groups required significantly less mechanical ventilation and had a lower mortality rate than the control group. Decontamination with paraffin oil could be advantageous for reducing the severity of AlP poisoning, improving prognosis, and lowering the mortality rate.
Topics: Humans; Male; Female; Adult; Pesticides; Single-Blind Method; Aluminum Compounds; Phosphines; Mineral Oil; Biomarkers; Poisoning
PubMed: 36592154
DOI: 10.1177/09603271221149650 -
International Journal of Environmental... Dec 2022Excipients are used as vehicles for topical treatments; however, there are not many studies that evaluate the impact of different excipients themselves. The aim of this...
Excipients are used as vehicles for topical treatments; however, there are not many studies that evaluate the impact of different excipients themselves. The aim of this research is to assess skin homeostasis changes in healthy individuals after using water/oil (W/O), oil/water (O/W), Beeler base, foam and Vaseline excipients. A within-person randomized trial was conducted that included healthy individuals without previous skin diseases. Skin barrier function parameters, including stratum corneum hydration (SCH), transepidermal water loss (TEWL), pH, temperature, erythema, melanin and elasticity (R0, R2, R5 and R7), were measured on the volar forearm before and after using each excipient. Sixty participants were included in the study, with a mean age of 32 years. After applying w/o excipient erythema decreased by 25 AU, ( < 0.001) and elasticity increased by 6%. After using the o/w excipient, erythema decreased by 39.36 AU ( < 0.001) and SCH increased by 6.85 AU ( = 0.009). When applying the Beeler excipient, erythema decreased by 41.23 AU ( < 0.001) and SCH increased by 15.92 AU ( < 0.001). Foam and Vaseline decreased TEWL and erythema. Excipients have a different impact on skin barrier function. Knowing the effect of excipients on the skin could help to develop new topical treatments and help specialists to choose the best excipient according to the pathology.
Topics: Humans; Adult; Excipients; Skin; Petrolatum; Administration, Topical; Erythema
PubMed: 36554558
DOI: 10.3390/ijerph192416678 -
Journal of Agricultural and Food... Dec 2022Mineral oil aromatic hydrocarbons (MOAHs) include mutagenic and carcinogenic substances and are considered a potential health risk. Current methods address the total...
Mineral oil aromatic hydrocarbons (MOAHs) include mutagenic and carcinogenic substances and are considered a potential health risk. Current methods address the total MOAH content but cannot address the actual toxicological hazard of individual components. This work presents a combined methodology closing those gaps: high-performance liquid chromatography (HPLC) coupled to gas chromatography with flame ionization detection was used to determine the MOAH content. To characterize present substance classes, comprehensive two-dimensional gas chromatography with time-of-flight mass spectrometry was applied. Preparative HPLC separated MOAHs into subgroups, which were tested with a miniaturized Ames test evaluating DNA reactivity of isolated fractions. Combining these methods allowed a correlation between present subgroups and DNA reactivity. The developed approach was applied to a mineral oil and distinguished between not DNA-reactive mono- and diaromatics and DNA-reactive tri- and polyaromatics, providing a proof of concept. Hereinafter, it will be applied to diverse sample matrices including mineral oils, food, and food contact materials.
Topics: Mineral Oil; Food Contamination; Gas Chromatography-Mass Spectrometry; Hydrocarbons, Aromatic; Flame Ionization
PubMed: 36524957
DOI: 10.1021/acs.jafc.2c05970 -
European Review For Medical and... Dec 2022Dexpanthenol is an ingredient in multiple topical pharmaceutical preparations thanks to its high penetration and localized concentration. It is included in many...
OBJECTIVE
Dexpanthenol is an ingredient in multiple topical pharmaceutical preparations thanks to its high penetration and localized concentration. It is included in many ointments or lotions for dermatological use, assisting in healing and reducing pruritus. Vaseline is a synthetic product obtained by distilling crude oil. It is commercially available in several grades. The study presented here examined how topically applied agents (dexpanthenol or vaseline) affect nasal epithelial cells in culture. In particular, the study aimed to identify any alterations to epithelial cells which might indicate toxicity.
MATERIALS AND METHODS
The nasal epithelial cells used were sourced from mucosal tissue fragments left over the following septorhinoplasty on five patients not suffering from rhinosinusitis. The first step was to dissect the mucosal fragments into smaller pieces on a sterilized Petri dish. These fragments were then placed into the DMEM-F12 cell culture medium, which had been freshly prepared. The dexpanthenol and vaseline were diluted in dimethylsulfoxide (DMSO) to a concentration of 5 mg/mL. The cells in the wells were exposed to varying concentrations of dexpanthenol or vaseline. The actual concentration of the test reagent to which the epithelial cells were exposed ranged from 0.15 mg/mL to 5 mg/mL. The exposure period was 24 hours. The cells were finally examined using a Leica SP5II confocal microscope. The features sought were DNA fragmentation, condensation of the nuclei, changes in the outer membrane, or cytoskeletal abnormality. These features, if present, indicate cytotoxicity.
RESULTS
The viability of the cultured nasal epithelial cells was unaltered by a 24-hour exposure to dexpanthenol, nor was the cellular proliferation rate affected at the level of statistical significance. There was evidence of a cytotoxic effect from exposing nasal epithelial cells to vaseline in liquid form for 24 hours. There was a reduction in cellular viability in the plates where the highest dose of vaseline (5 mg/mL) was used. Cellular viability was not affected significantly at any of the doses below 5 mg/mL.
CONCLUSIONS
The absence of cytotoxic effects from the application of dexpanthenol to the nasal mucosa indicates that this agent may be safely used within the nose. The cytotoxic effects of liquid vaseline observed in this trial (condensed nuclear chromatin, loss of cellular volume) indicate that this agent may be harmful when used intranasally. For patients who require nasal packing due to nose bleeds or following endoscopic sinus surgical procedures, dexpanthenol should be preferred to vaseline from the point of view of maximizing healing of a nasal injury.
Topics: Humans; Excipients; Petrolatum; Sinusitis; Pantothenic Acid
PubMed: 36524920
DOI: 10.26355/eurrev_202212_30496 -
Medicina Clinica Feb 2023
Topics: Humans; Pneumonia, Lipid; Oils; Mineral Oil; Paraffin
PubMed: 36464511
DOI: 10.1016/j.medcli.2022.10.008 -
Regulatory Toxicology and Pharmacology... Jan 2023Mineral oils (food grade white oil or liquid paraffin) have historically been safely used in a number of sensitive end-uses, including pharmaceutical, cosmetic and food.... (Review)
Review
Mineral oils (food grade white oil or liquid paraffin) have historically been safely used in a number of sensitive end-uses, including pharmaceutical, cosmetic and food. Recent concern that certain mineral hydrocarbons (branched and cyclo-alkanes) may accumulate in human tissues has prevented European Food Safety Authority (EFSA) from deriving guidance values for food exposures. Analysis of human and animal tissue indicate that an unresolved cloud of mostly highly branched alkanes and alkylated cycloalkanes within the C20-C35 range is consistently present in all tissues. This critical review thoroughly assesses the retention of "mineral oil saturated hydrocarbons" (MOSH) in human and animal tissues and evaluates if the presence of MOSH is considered adverse and appropriate to use for risk assessment, generation of guidance values for food exposure and/or generation of derivation of health-based guidance values. An adversity framework was utilized to perform an in-depth weight of the evidence analysis, and it was concluded that mere presence of MOSH does not translate to hazard identification, and is not considered adverse. In light of this conclusion, it would not be appropriate to utilize this endpoint as the point of departure to calculate a health guidance value.
Topics: Animals; Humans; Mineral Oil; Hydrocarbons; Cosmetics; Food Safety; Alkanes; Food Contamination
PubMed: 36402242
DOI: 10.1016/j.yrtph.2022.105284 -
Analytica Chimica Acta Nov 2022Contamination of foods with mineral oil hydrocarbons, particularly mineral oil aromatic hydrocarbons (MOAH), can potentially pose a health hazard to consumers. However,... (Review)
Review
Contamination of foods with mineral oil hydrocarbons, particularly mineral oil aromatic hydrocarbons (MOAH), can potentially pose a health hazard to consumers. However, identifying toxic substances among the many thousands of compounds comprising mineral oils in food samples is a difficult analytical challenge. According to the European Food Safety Authority, there is a lack of concentration and structural data about mineral oil hydrocarbons in foods, and therefore it is not clear to what extent consumers in Europe might be exposed to toxic levels of MOAH. The current gold standard method for determination of mineral oil hydrocarbons is online high-performance liquid chromatography (LC)-gas chromatography (GC) with flame ionization detection, which quantifies total saturated/aromatic content, but gives no qualitative information. The objective of this review is to explore the future prospects in mineral oil hydrocarbon determination and MOAH characterization in foods. To that end, peer reviewed literature was explored, particularly from the viewpoint of a methodology for detailed characterization of the MOAH fraction that can aid toxicological assessment. The literature clearly shows that there is much to be gained from the orthogonality power of multidimensional chromatographic separations and mass spectrometric (MS) detection. Comprehensive two-dimensional GC coupled to MS, preceded by pre-fractionations of MOAH by LC is suggested to be the most promising approach for further research. In addition, the strengths and weaknesses of a number of other, alternative approaches, both for qualitative and quantitative analysis, are discussed.
Topics: Mineral Oil; Food Contamination; Gas Chromatography-Mass Spectrometry; Hydrocarbons, Aromatic; Hydrocarbons
PubMed: 36328715
DOI: 10.1016/j.aca.2022.340098 -
International Journal of Environmental... Oct 2022An excess of body fat is one of the biggest public health concerns in the world, due to its relationship with the emergence of other health problems. Evidence suggests... (Randomized Controlled Trial)
Randomized Controlled Trial
Krill Oil Supplementation Does Not Change Waist Circumference and Sagittal Abdominal Diameter in Overweight Women: A Pilot Balanced, Double-Blind, and Placebo-Controlled Clinical Trial.
An excess of body fat is one of the biggest public health concerns in the world, due to its relationship with the emergence of other health problems. Evidence suggests that supplementation with long-chain polyunsaturated fatty acids (omega-3) promotes increased lipolysis and the reduction of body mass. Likewise, this clinical trial aimed to evaluate the effects of supplementation with krill oil on waist circumference and sagittal abdominal diameter in overweight women. This pilot, balanced, double-blind, and placebo-controlled study was carried out with 26 women between 20 and 59 years old, with a body mass index >25 kg/m. The participants were divided into the control (CG) ( = 15, 3 g/daily of mineral oil) and krill oil (GK) ( = 16, 3 g/daily of krill oil) groups, and received the supplementation for eight weeks. Food intake variables were obtained using a 24 h food recall. Anthropometric measurements (body mass, body mass index, waist circumference, and sagittal abdominal diameter) and handgrip strength were obtained. After the intervention, no changes were found for the anthropometric and handgrip strength variables ( > 0.05). Regarding food intake, differences were found for carbohydrate ( = 0.040) and polyunsaturated ( = 0.006) fatty acids, with a reduction in the control group and an increase in krill oil. In conclusion, supplementation with krill oil did not reduce the waist circumference and sagittal abdominal diameter. Therefore, more long-term studies with a larger sample size are necessary to evaluate the possible benefits of krill oil supplementation in overweight women.
Topics: Animals; Humans; Female; Young Adult; Adult; Middle Aged; Overweight; Waist Circumference; Euphausiacea; Sagittal Abdominal Diameter; Hand Strength; Mineral Oil; Dietary Supplements; Fatty Acids, Omega-3; Double-Blind Method; Carbohydrates
PubMed: 36294155
DOI: 10.3390/ijerph192013574 -
A novel monocyte differentiation pattern in pristane-induced lupus with diffuse alveolar hemorrhage.ELife Oct 2022Pristane causes chronic peritoneal inflammation resulting in lupus, which in C57BL/6 mice is complicated by lung microvascular injury and diffuse alveolar hemorrhage...
Pristane causes chronic peritoneal inflammation resulting in lupus, which in C57BL/6 mice is complicated by lung microvascular injury and diffuse alveolar hemorrhage (DAH). Mineral oil (MO) also causes inflammation, but not lupus or DAH. Since monocyte depletion prevents DAH, we examined the role of monocytes in the disease. Impaired bone marrow (BM) monocyte egress in /- mice abolished DAH, confirming the importance of monocyte recruitment to the lung. Circulating Ly6C monocytes from pristane-treated mice exhibited increased annexin-V staining in comparison with MO-treated controls without evidence of apoptosis, suggesting that pristane alters the distribution of phosphatidylserine in the plasma membrane before or shortly after monocyte egress from the BM. Plasma membrane asymmetry also was impaired in Nr4a1-regulated Ly6C 'patrolling' monocytes, which are derived from Ly6C precursors. Patrolling Ly6C monocytes normally promote endothelial repair, but their phenotype was altered in pristane-treated mice. In contrast to MO-treated controls, Nr4a1-regulated Ly6C monocytes from pristane-treated mice were CD138, expressed more TremL4, a protein that amplifies TLR7 signaling, and exuberantly produced TNFα in response to TLR7 stimulation. TremL4 expression on these novel CD138 monocytes was regulated by Nr4a1. Thus, monocyte CD138, high TremL4 expression, and annexin-V staining may define an activated/inflammatory subtype of patrolling monocytes associated with DAH susceptibility. By altering monocyte development, pristane exposure may generate activated Ly6C and Ly6C monocytes, contributing to lung microvascular endothelial injury and DAH susceptibility.
Topics: Mice; Animals; Monocytes; Mice, Inbred C57BL; Tumor Necrosis Factor-alpha; Mineral Oil; Phosphatidylserines; Toll-Like Receptor 7; Lung Diseases; Hemorrhage; Inflammation; Annexins
PubMed: 36264674
DOI: 10.7554/eLife.76205