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Pediatric Health, Medicine and... 2023The most important anemia next to iron deficiency is anemia of inflammation. Micronutrient deficits, such as those in zinc and iron, can be caused by intestinal...
BACKGROUND
The most important anemia next to iron deficiency is anemia of inflammation. Micronutrient deficits, such as those in zinc and iron, can be caused by intestinal permeability and gut inflammation brought on by environmental enteric dysfunction. This study was aimed to evaluate the prevalence and association of anemia with Environmental Enteropathy.
METHODS
Data on water sanitation and hygiene indicators and sociodemographic characteristics were collected using structured questionnaire. The lactulose to mannitol ratio (L:M) was calculated from the concentration of both sugars in the urine. Level of Hemoglobin was detected by using Hemocue-301 digital photometer. Blood and urine sample was collected from three hundred children aged 12-59 months to determine the status of Anaemia and Environmental Enteropathy respectively.
RESULTS
Data were analyzed by using Descriptive statistics, cross-tabulation, and logistic regression model to indicate prevalence and association of anemia with environmental Enteropathy in children less than five years old. The prevalence of anemia in children with environmental enteropathy was 63.8% (95% CI: 57.6, 71.7), and there was a significant association (p = 0.0001, AOR 3.502, 95% CI: 1.929-6.371) between anemia and environmental enteropathy. In a multivariate analysis, children aged 1-3 years with caretakers who had no or only primary education and with monthly income of less than 3000 ETB were more likely to develop anemia.
CONCLUSION
The result of this study indicated that two-thirds of children less than five with environmental enteropathy had developed anemia, and there is a significant association between environmental enteropathy and anemia. Even though there are other causes of anemia, based on the findings of this study, more research is needed to identify factors associated with environmental enteropathy to mitigate anemia due to intestinal permeability or malabsorption and its impact in children under the age of five.
PubMed: 36798588
DOI: 10.2147/PHMT.S387747 -
Orthopaedic Surgery Apr 2023Leucocyte esterase (LE) strip test is the most rapid, convenient, and cheap method to diagnose chronic periprosthesis joint infection (PJI). However, the determination...
OBJECTIVE
Leucocyte esterase (LE) strip test is the most rapid, convenient, and cheap method to diagnose chronic periprosthesis joint infection (PJI). However, the determination of LE strip mainly relies on colorimetric method with strong subjectivity, which leads to low diagnostic accuracy. Therefore, we try to convert LE strip images into digital data through the RGB photometric system to achieve objective diagnosis. This method will greatly improve the accuracy of LE strip detection and diagnosis of PJI.
METHODS
From January 2021 to September 2021, 46 patients with suspected PJI after total hip and knee arthroplasty underwent diagnostic joint puncture. After effective joint fluid samples were harvested, they were divided into original fluid and centrifuged fluid for LE strip detection. Real-time images of LE strip were taken at 90 s, 3 min, 5 min, 10 min, and 15 min after sampling, and their brightness (Y) was obtained after they were input into an RGB photometric system. Grouping was based on centrifugation, infection, and time points, and then the differences in brightness among groups were compared. The correlation between LE strip image brightness and WBC count was evaluated. Student t-test was used for the parametric data and chi-square test for qualitative data. Simple linear regression was utilized to analyze the correlation between brightness and WBC count in each group.
RESULTS
Included were 19 cases of PJI and 27 Non-PJI subjects diagnosed against ICM2018 diagnostic criteria. The brightness was lower in the PJI group than in Non-PJI group (p < 0.05). The brightness of the uncentrifuged group was lower than that of the centrifuged group (p < 0.05). Irrespective of centrifugation or infection, the brightness of LE strip decreased with the exposure time after sampling. The brightness of LE strip was correlated with WBC count at different time points, with the correlation being strongest 5 min after sampling (R (5 min) = 0.86, p < 0.0001). The correlation between LE strip brightness and WBC count was also found in the centrifugation group, with the correlation being most robust 15 min after sampling (R (15 min) = 0.73, p < 0.0001).
CONCLUSION
A remarkable correlation was found between LE strip brightness and the WBC count. It is feasible to directly quantify LE strip image on a RGB photometer to achieve quantitative detection of LE strip to diagnose PJI.
Topics: Humans; Prosthesis-Related Infections; Synovial Fluid; Arthroplasty, Replacement, Knee; Carboxylic Ester Hydrolases; Arthritis, Infectious; Biomarkers; Sensitivity and Specificity
PubMed: 36782275
DOI: 10.1111/os.13667 -
Journal of Anaesthesiology, Clinical... 2022Blood loss in neurosurgical procedures can be rapid and tremendous leading to consequential hemodynamic instability. HemoCue is a portable photometer used for the...
BACKGROUND AND AIMS
Blood loss in neurosurgical procedures can be rapid and tremendous leading to consequential hemodynamic instability. HemoCue is a portable photometer used for the measurement of blood hemoglobin concentration. Using this point of care device, we contemplated this study to assess the reliability of HemoCue for suction hemoglobin determination and calculation of surgical blood loss by comparing with the gold standard laboratory Coulter Counter method in patients undergoing neurosurgical procedures.
MATERIAL AND METHODS
This prospective observational study was conducted in 233 patients undergoing elective neurosurgical procedures in the age group of 18 to 60 years and having preoperative hemoglobin (Hb) values above 9 g/dL. Values of preoperative hemoglobin, suction hemoglobin, and volume in the suction container were used to obtain the estimated blood loss. The normality of the data was tested using the Kolmogorov Smirnov test. Bland Altman analysis was used to test the reliability of HemoCue in estimating hemoglobin and blood loss with reference to the gold standard laboratory Coulter Counter automated hematology analyzer.
RESULTS
Median blood loss calculated in our study using HemoCue was 554.65 mL with an interquartile range of 336.81 mL to 982.39 mL. Laboratory counter method estimated median blood loss was 533.37 mL with an interquartile range of 335.21 mL to 994.73 mL. The majority of the data obtained and analyzed using the Bland and Altman analysis method were within a 95% confidence interval.
CONCLUSION
The HemoCue method is a reliable method and henceforth can be used to estimate blood loss in suction fluid in patients undergoing neurosurgical procedures.
PubMed: 36778832
DOI: 10.4103/joacp.JOACP_638_20 -
The Science of the Total Environment May 2023Dust particles originating from arid desert regions can be transported over long distances, presenting severe risks to climate, environment, social economics, and human...
Dust particles originating from arid desert regions can be transported over long distances, presenting severe risks to climate, environment, social economics, and human health at the source and downwind regions. However, there has been a dearth of continuous diurnal observations of vertically resolved mass concentration and optical properties of dust aerosols, which hinders our understanding of aerosol mixing, stratification, aerosol-cloud interactions, and their impacts on the environment. To fill the gap of the insufficient observations, to the best of our knowledge, this work presents the first high-spectral-resolution lidar (HSRL) observation providing days of continuous profiles of the mass concentration, along with particle linear depolarization ratio (PLDR), backscattering coefficient, extinction coefficient and lidar ratio (LR), simultaneously. We present the results of two strong dust events observed by HSRL over Beijing in 2021. The maximum particle mass concentrations reached (1.52 ± 3.5) x10 μg/m and (19.48 ± 0.36) x10 μg/m for the two dust events, respectively. The retrieved particle mass concentrations and aerosol optical depth (AOD) agree well with the observation from the surface PM concentrations and sun photometer with correlation coefficients of 0.90 and 0.95, respectively. The intensive properties of PLDR and LR of the dust aerosols are 0.31 ± 0.02 and 39 ± 7 sr at 532 nm, respectively, which are generally close to those obtained from observations in the downwind areas. Moreover, inspired by the observations from HSRL, a universal analytical relationship is discovered to evaluate the proportion of dust aerosol backscattering, extinction, AOD, and mass concentration using PLDR. The universal analytical relationship reveals that PLDR can directly quantify dust aerosol contribution, which is expected to further expand the application of polarization technology in dust detection. These valuable observations and findings further our understanding of the contribution of dust aerosol to the environment and help supplement dust aerosol databases.
PubMed: 36758704
DOI: 10.1016/j.scitotenv.2023.162091 -
Diagnostics (Basel, Switzerland) Jan 2023This article systematizes available data from the literature on biliary gastritis (BG) in order to increase the awareness of specialists about the latest possibilities... (Review)
Review
This article systematizes available data from the literature on biliary gastritis (BG) in order to increase the awareness of specialists about the latest possibilities for diagnosing the disease. BG occurs as a result of pathological duodenogastric reflux. In patients with a preserved duodenogastric junction, the dominant factor is represented by motor disorders of the upper digestive tract (primary biliary gastritis), while in patients recovering from surgical interventions it is represented by structural changes (secondary biliary gastritis). Progressive BG can lead to atrophy of the gastric mucosa, intestinal metaplasia, epithelial dysplasia, and eventually to gastric cancer. Diagnostic methods for BG are carried out to identify risk factors, exclude alarm symptoms and identify persistent motor disorders and pathological reflux (24 h pH-impedancemetry, hepatobiliary scintigraphy, 24 h monitoring of bilirubin content in the reflux using a Bilitec 2000 photometer), as well as to diagnose gastritis itself (esophagogastroduodenoscopy, morphological gastrobiopsy examination). The diagnosis of BG should be based on a multidisciplinary approach that combines a thorough analysis of a patient's complaints, an anamnesis of the disease, and the results of endoscopic and histological research methods.
PubMed: 36672996
DOI: 10.3390/diagnostics13020186 -
Saudi Journal of Ophthalmology :... 2022Aqueous flare and cells are inflammatory parameters of anterior chamber inflammation resulting from disruption of the blood-ocular barrier. The ocular inflammation...
Aqueous flare and cells are inflammatory parameters of anterior chamber inflammation resulting from disruption of the blood-ocular barrier. The ocular inflammation related to anterior chamber cells and flare is assessed by conventional clinical grading systems through using the slit-lamp examination. However, a more quantitative and objective assessment is needed for more precise and reproducible inflammatory assessment in uveitis. Laser flare photometer (LFP) was introduced as a noninvasive, objective, and quantitative evaluation of aqueous flare intensity and number of cells in the anterior chamber with good accuracy and repeatability. The success of LFP allowed clinicians to further evaluate the pathophysiology of intra-ocular inflammation and to incorporate LFP measurements to their routine clinical practice for diagnosis, management, and treatment of uveitis cases. In this review, we will discuss the importance of clinical utilization of LFP and the correlation between LFP and clinical grading systems along with some technical aspects. Furthermore, we will give a literature summary on the current applications of LFP in clinical practice of cases which present with various types of uveitis and diverse ocular conditions with or without inflammation.
PubMed: 36618569
DOI: 10.4103/sjopt.sjopt_119_22 -
Toxicology Reports 2022The recent expansion of industries in Addis Ababa is causing additional environmental pollution through wastewater discharges; this is becoming a critical concern. Addis...
The recent expansion of industries in Addis Ababa is causing additional environmental pollution through wastewater discharges; this is becoming a critical concern. Addis Ababa is located in the upper Awash River basin, and is the main source of industrial pollutants to the river. In this study, physicochemical parameters, nutrients and heavy metal content of wastewaters released from 16 factories, 6 tanneries, 6 beverages and 4 diverse factories, and the Akaki-Kality central wastewater treatment plant in Addis Ababa, were sampled to assess the level of pollutants. Heavy metals were determined using inductively coupled plasma optical emission spectroscopy (ICP-OES). Analysis of nutrients were conducted using Palintest Photometer. Physicochemical characteristics were measured either in situ using a portable micro meter or in the laboratory. Among the measured physicochemical properties, critical issues were observed with electrical conductivity, total dissolved solids and total hardness. Effluents from all of the tanneries, and a number of other factories, were found at levels higher than the maximum limits of various guideline standards. In addition, samples from two of the tanneries (T1 and T5), two beverage factories (B3 and B6) and the central wastewater treatment plant showed elevated concentrations of PO , which violated the limit (10 mg/l) set by Environmental Protection Agency of Ethiopia (ETHEPA). The two tanneries (T1 and T5) also contained higher SO than the guideline limit of 1000 mg/l. On the other hand, only one factory, one brewery (B3), exhibited NO above the standard limit of 20 mg/l. Whereas NH, NH , Cl, S and NO were within the limits in all of the samples. Severe pollution was found in wastewaters from tanneries, where half of them (T1, T5 and T6) contained Cr beyond the maximum limit of 2000 µg/l. Furthermore, a third of the tanneries (T1 and T5) and a beverage factory (B5) contained Fe, Mn, Zn and Cu, higher than the ETHEPA limits of 10000, 5000, 5000 and 2000 µg/l, respectively. Waste disposal from factories without proper treatment can cause great harm to the local people and the environment. Hence, the results of this study call for regulatory bodies to pay close attention to factories, particularly tanneries, in Addis Ababa in implementing adequate treatments of their wastewater discharges.
PubMed: 36518430
DOI: 10.1016/j.toxrep.2022.06.002 -
Toxicology Reports 2022The rapid development associated with Next Generation Tobacco Products (NGTP) has necessitated the development of high throughput methodologies to test their genotoxic...
Characterization of smoke and aerosol deliveries from combustible cigarettes, heated tobacco products and electronic nicotine delivery systems in the Vitrocell® Mammalian 6/48 exposure module.
The rapid development associated with Next Generation Tobacco Products (NGTP) has necessitated the development of high throughput methodologies to test their genotoxic potential in vitro when compared to conventional cigarette smoke (CS). An assessment of two Vitrocell® Mammalian 6/48 exposure modules in three independent experiments was made by comparing results from multiple dosimetric techniques applied to aerosol generated from 3R4F Kentucky Reference cigarettes, commercially available electronically heated tobacco product (eHTP) and Electronic Nicotine Delivery System (ENDS) using the Vitrocell® VC10®. Real-time aerosol particle concentration was assessed by means of light scattering photometers and expressed as area under the curve (∑AUC). Nicotine concentrations were determined analytically by LC/MS. Humectant amount and distribution was assessed for eHTP and ENDS by the quantification of free glycerol in a phosphate buffered saline (PBS) trap, whereas total particulate matter (TPM) was assessed in the 3R4F cigarettes by the fluorescence of the particulate at 485 nm in anhydrous dimethyl sulfoxide (DMSO) trap within the exposure. Dose was adjusted by means of the addition of ambient air to dilute the whole smoke/aerosol in L/min and sampled into the system at a rate of 5 mL/min. Dilution of CS ranged from 8.0 to 0.5 L/min and for the eHTP and ENDS ranged from 4 to 0 L/min (undiluted). Dosimetric analysis of the system showed good concordance within replicates (p-values ranged from p = 0.3762 to p = 0.8926) and showed that the Vitrocell® Mammalian 6/48 is a viable means for genotoxic assessment of aerosol generated from both conventional cigarettes and NGTP. Results demonstrate the need to tailor dosimetry approaches to different aerosols due to variations in the physio-chemical composition, with a multi-dosimetry approach recommended.
PubMed: 36518380
DOI: 10.1016/j.toxrep.2022.11.001 -
International Journal of Environmental... 2023One of the greatest environmental risks in the cement industry is particulate matter emission (i.e., PM and PM). This paper aims to develop descriptive-analytical...
UNLABELLED
One of the greatest environmental risks in the cement industry is particulate matter emission (i.e., PM and PM). This paper aims to develop descriptive-analytical solutions for increasing the accuracy of predicting particulate matter emissions using resample data of Kerman cement plant. Photometer instruments DUST TRAK and BS-EN-12341 method were used to determine concentration of PM and PM. Sampling was performed on 4 environmental stations of Kerman cement plant in the four seasons. In order to accurate assessment of particulate matter concentration, a new model was proposed to resample cement plant time series data using Pandas in Python. The effect of meteorological parameters including wind speed, relative humidity, air temperature and rainfall on the particulate matter concentration was investigated through statistical analysis. The results indicated that the maximum annual average of 24-h of PM belonged to the east side (opposite the clinker depot) in 2019 (31.50 μg m) and west side (in front of the mine) in 2020 (31.00 μg m). Also, maximum annual average of 24-h of PM belonged to the west side (in front of the mine) in 2020 (121.00 μg m) and east side (opposite the clinker depot) in 2020 (120.75 μg m). The PM and PM concentrations are more than the allowable limit. The results demonstrate that particulate matter concentration increases with increasing relative humidity and rainfall. Finally, the SARIMA model was used to predict the particulate matter concentration.
SUPPLEMENTARY INFORMATION
The online version contains supplementary material available at 10.1007/s13762-022-04645-3.
PubMed: 36405244
DOI: 10.1007/s13762-022-04645-3 -
Sensors (Basel, Switzerland) Oct 2022A novel portable low-cost Arduino-controlled photo- and fluorimeter for on-site measurements has been developed. The device uses LEDs as a light source and a...
A novel portable low-cost Arduino-controlled photo- and fluorimeter for on-site measurements has been developed. The device uses LEDs as a light source and a phototransistor as a light sensor. The circuit is based on the discharge of a capacitor with the photocurrent from the phototransistor. Validation experiments for absorbance measurements were performed by measuring protein concentration using the Bradford method and measuring phosphate ions in water using a commercial test kit. The emission light of the excited fluorescent dyes rhodamine 6G and calcofluor white was measured to validate the usability of the device as a fluorescence photometer. In all validation experiments, similar correlation coefficients and limit of detection could be achieved with the portable photo- and fluorimeter and a laboratory spectrometer and fluorimeter. Real sample analysis was performed, measuring phosphate concentration in freshwater and concentration of green fluorescent protein, extracted from
Topics: Fluorescent Dyes; Green Fluorescent Proteins; Photometry; Water; Phosphates
PubMed: 36298268
DOI: 10.3390/s22207916