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Journal of Orthopaedic Surgery and... Jun 2024We aimed to analyze the outcomes of intraarticular extra virgin olive oil (EVOO) injection on mechanically induced rabbit knee osteoarthritis (OA) by studying the...
BACKGROUND
We aimed to analyze the outcomes of intraarticular extra virgin olive oil (EVOO) injection on mechanically induced rabbit knee osteoarthritis (OA) by studying the morphological, histological, and radiological findings.
METHODS
The study was conducted on 32 New Zealand White rabbits. The randomly numbered subjects were divided into two main groups. The rabbits numbered 1 to 16 were selected to be the group to receive EVOO, and the remaining were selected into a control group. Both groups were separated into two subgroups for short-term (five weeks) and long-term (10 weeks) follow-up. Anterior cruciate ligament transection was applied on the left knees of all the rabbits via medial parapatellar arthrotomy to simulate knee instability. Immediately after the surgical procedure, 0.2 cc of EVOO was injected into the knee joint of rabbits numbered 1-16, and the control group received 0.2 cc of sterile saline. On the 14th day, long-term group subjects were administered another dose of 0.2 cc EVOO intraarticularly.
RESULTS
The gross morphological scores of the control group subjects were significantly different from the EVOO group for both short-term (p = 0,055) and long-term (p = 0,041) scores. In parallel, the MRI results of the EVOO subjects were significantly different from the control group for both short-term and long-term follow-up assessment scores (p = 0.017, p = 0.014, respectively). The Mankin scoring results showed that there were statistically significant differences between the EVOO and control group in the comparison of both total scores (p = 0.001 for short-term and p = 0.004 for long-term) and subgroup scoring, including macroscopic appearance, chondrocyte cell number, staining, and Tidemark integrity in both short-term (p = 0.005, p = 0.028, p = 0.001, p = 0.005, respectively) and long-term assessments (p = 0.002, p = 0.014, p < 0.001, p = 0. 200, respectively).
CONCLUSIONS
We have observed promising outcomes of intra-articular application of extra virgin olive oil in the treatment of acute degenerative osteoarthritis in rabbit knees. Due to its potential cartilage restorative and regenerative effects, EVOO, when administered intra-articularly, may be a promising agent to consider for further research in the treatment of OA.
Topics: Rabbits; Animals; Olive Oil; Injections, Intra-Articular; Osteoarthritis, Knee; Male
PubMed: 38849876
DOI: 10.1186/s13018-024-04818-5 -
BMC Complementary Medicine and Therapies Jun 2024The chemical composition and biological activities of Eucalyptus essential oils (EOs) have been documented in numerous studies against multiple infectious diseases. The...
BACKGROUND
The chemical composition and biological activities of Eucalyptus essential oils (EOs) have been documented in numerous studies against multiple infectious diseases. The antibacterial activity of individual Eucalyptus EOs against strains that cause ear infections was investigated in our previous study. The study's antibacterial activity was promising, which prompted us to explore this activity further with EO blends.
METHODS
We tested 15 combinations (9 binary combinations and 6 combinations of binary combinations) of Eucalyptus EOs extracted by hydrodistillation from eight Tunisian Eucalyptus species dried leaves against six bacterial strains responsible for ear infections: three bacterial isolates (Haemophilus influenzae, Haemophilus parainfluenzae, and Klebsiella pneumoniae) and three reference bacteria strains (Pseudomonas aeruginosa, ATTC 9027; Staphylococcus aureus, ATCC 6538; and Escherichia coli, ATCC 8739). The EOs were analyzed using GC/FID and GC/MS. The major compounds, as well as all values obtained from the bacterial growth inhibition assay, were utilized for statistical analysis.
RESULTS
The antibacterial activity of the EO blends exhibited significant variation within Eucalyptus species, bacterial strains, and the applied methods. Principal component analysis (PCA) and hierarchical cluster analysis (HCA), based on the diameters of the inhibition zone, facilitated the identification of two major groups and ten subgroups based on the level of antibacterial activity. The highest antibacterial activity was observed for the mixture of EOs extracted from E. panctata, E. accedens, and E. cladoclayx (paac) as well as E. panctata, E. wandoo, E. accedens, and E. cladoclayx (pwac) using the disc diffusion method. Additionally, significant activity was noted with EOs extracted from E. panctata, E. wandoo (pw) and E. panctata, E. accedens (pa) using the broth microdilution method.
CONCLUSION
Our findings suggest that certain EO combinations (paac, pwac, pw, and pa) could be considered as potential alternative treatment for ear infections due to their demonstrated highly promising antibacterial activities.
Topics: Eucalyptus; Oils, Volatile; Anti-Bacterial Agents; Microbial Sensitivity Tests; Humans; Bacterial Infections; Bacteria; Plant Oils
PubMed: 38849805
DOI: 10.1186/s12906-024-04494-2 -
BMC Plant Biology Jun 2024This study investigates a novel idea about the foliar application of nanoparticles as nanofertilizer combined with a natural stimulant, blue-green algae Spirulina...
BACKGROUND
This study investigates a novel idea about the foliar application of nanoparticles as nanofertilizer combined with a natural stimulant, blue-green algae Spirulina platensis L. extract, as a bio-fertilizer to achieve safety from using nanoparticles for enhancement of the growth and production of the plant. Thus, this experiment aimed to chemically synthesize copper nanoparticles via copper sulfate in addition to evaluate the impact of CuNPs at 500, 1000, and 1500 mg/L and the combination of CuNPs with or without microalgae extract at 0.5, 1, and 1.5 g/L on the morphological parameters, photosynthetic pigments accumulation, essential oil production, and antioxidant activity of French basil.
RESULTS
The results revealed that foliar application of CuNPs and its interaction with spirulina extract significantly increased growth and yield compared with control, the treatments of 1000 and 1500 mg/L had less impact than 500 mg/L CuNPs. Plants treated with 500 mg/L CuNPs and 1.5 g/L spirulina extract showed the best growth and oil production, as well as the highest accumulation of chlorophylls and carotenoids. The application of CuNPs nanofertilizer caused a significant increase in the antioxidant activity of the French basil plant, but the combination of CuNPs with spirulina extract caused a decrease in antioxidant activity.
CONCULOSION
Therefore, foliar application of natural bio-fertilizer with CuNPsis necessary for obtaining the best growth and highest oil production from the French basil plant with the least damage to the plant and the environment.
Topics: Spirulina; Copper; Ocimum basilicum; Metal Nanoparticles; Antioxidants; Plant Leaves; Fertilizers; Chlorophyll; Photosynthesis; Oils, Volatile
PubMed: 38849727
DOI: 10.1186/s12870-024-05153-x -
Scientific Reports Jun 2024This study aimed to evaluate the impact of thyme and/or garlic oil administration on growth performance, immunity, antioxidant, biochemical parameters, and net farm...
This study aimed to evaluate the impact of thyme and/or garlic oil administration on growth performance, immunity, antioxidant, biochemical parameters, and net farm income of Damascus goats. Forty weaned Damascus goats were allocated into four groups. The first group was the control without oral administration, while the 2nd (Th), 3rd (Gr), and 4th (ThGr) groups were orally administrated by (2 ml/goat/day) of thyme oil, garlic oil and their mixture (1:1), respectively during the whole experiment period. The final body weight of goats orally administered oil mixture was the heaviest group, it was 10, 4.5 and 3.5% than the control, Th. and Gr. groups, respectively with better feed conversion ratio and high net farm income. Goats of ThGr. group revealed the best immunity, antioxidant and general health condition than the control group with 50% reduction of MDA. Liver (AST, 33% and ALT, 38%) and kidney (creatinine, 88%) functions improved by oils mixtures orally administration compared with the control group. LDL, triglyceride and cholesterol were reduced by 47, 33 and 21% compared with the control group, respectively. Thus, mixture oil administration (thyme and garlic at the ratio of 1:1, 2 ml/goat/day) improved growth (10%), antioxidant status (MDA 50%), liver (AST, 33% and ALT, 38%), kidney function (creatinine, 88%), the FCR (17.4%) and net farm income (21%), of Damascus goats.
Topics: Animals; Goats; Thymus Plant; Antioxidants; Plant Oils; Garlic; Animal Feed; Sulfides; Liver; Allyl Compounds
PubMed: 38849384
DOI: 10.1038/s41598-024-62417-0 -
Heliyon Jun 2024To extend the vase life of cut flowers, there is now a trend of using plant essential oils in place of synthetic chemicals, as they are fully biodegradable, more...
To extend the vase life of cut flowers, there is now a trend of using plant essential oils in place of synthetic chemicals, as they are fully biodegradable, more eco-friendly, and safer. The objective of this study was to examine the possible application and postharvest quality effects of three plant essential oils namely, ginger ( Roscoe), peppermint ( L.), and citronella ( Rendle), as natural vase solution for cut flowers. Peppermint essential oil showed promise as a holding solution for extending the vase life of orchids. To confirm vase life extension, emulsions containing peppermint essential oil at concentrations of 50 and 100 μg mL combined with 4 % glucose to formulate holding solutions applied to orchids. Vase life, some biochemical changes, electrolyte leakage, total microbial count in the holding solution, and physical condition via scanning electron microscopy (SEM) were evaluated over a period of 25 days. The three major compounds in peppermint essential oil were identified as menthol (33.24 %), 1-menthone (18.91 %) and menthofuran (14.85 %). The essential oil was applied in emulsion form as a holding solution. Treatment with 4 % glucose and either 50 or 100 μg mL peppermint essential oil prolonged the vase life of orchids to up to 28 days. Scanning electron microscopy on Day 7 showed that the xylem vessels of treated orchids remained clear, suggesting reduced microbial plugging at the stalk end. Similarly, on Day 20, a reduced microbial cell count was observed for treated orchids (<1 log CFU mL) in comparison with controls (7.20 ± 0.04 log CFU mL). Finally, the essential oil improved flower quality by helping preserve petal membrane stability and petal anthocyanin content. Our results suggest the application of peppermint essential oil as a novel alternative to chemicals used in holding solutions for extending the vase life of orchids.
PubMed: 38845939
DOI: 10.1016/j.heliyon.2024.e31636 -
Scientific Reports Jun 2024In this work, the surface of polyvinyl chloride PVC sheet was modified by blending it with sunflower seed oil SSO to obtain PVC sheet/SSO films of ratios 100/0, 90/10,...
In this work, the surface of polyvinyl chloride PVC sheet was modified by blending it with sunflower seed oil SSO to obtain PVC sheet/SSO films of ratios 100/0, 90/10, 80/20, 70/30, 60/40, and 50/50 (v/v)% using the solution casting method. Various techniques were used to characterize the prepared films, besides the use of hemolysis assays and blood clot formation tests. FTIR spectra revealed that there was a good interaction between the PVC sheet and the oil. The dielectric measurement indicated that SSO addition enhanced the dielectric properties of the sheet. The study of dielectric relaxation times confirmed the interaction between SSO and the sheet. DC conductivity increased to 6 × 10 S/m, so it could be applied in antistatic applications. Also, SSO addition increased the value of the thermal stability. According to SEM micrographs, the film was roughened at a ratio of 60/40 and smoothed out at 50/50. This behavior was confirmed with roughness and contact angle measurement results, in which the film of ratio 60/40 had the highest value equal to (72.03°) and then decreased at 50/50 to (59.62°). These results were confirmed by XRD measurement as the crystallinity increased at the film ratio of 60/40 and decreased again at 50/50. Also, the ratio of 60/40 demonstrated a large decrease in thrombus weights along with a slight increase in hemolysis, which is within the acceptable range and has a high degree of biocompatibility, so this concentration is recommended to be used in blood bags applications.
Topics: Sunflower Oil; Polyvinyl Chloride; Hemolysis; Spectroscopy, Fourier Transform Infrared; Humans; Animals; Blood Coagulation; Surface Properties; Plant Oils
PubMed: 38844516
DOI: 10.1038/s41598-024-62709-5 -
PloS One 2024This research presents a comprehensive study of sequential oxidative extraction (SOE) consisting of alkaline and acidic oxidation processes to extract nanocellulose from...
This research presents a comprehensive study of sequential oxidative extraction (SOE) consisting of alkaline and acidic oxidation processes to extract nanocellulose from plant biomass. This proposed process is advantageous as its operation requires a minimum process with mild solvents, and yet successfully isolated high-quality nanofibrillated cellulose (NFC) from raw OPEFB. The SOE involved ammonium hydroxide (NH4OH, 2.6 M) and formic acid (HCOOH, 5.3 M) catalyzed by hydrogen peroxide (H2O2, 3.2 M). This approach was used to efficiently solubilize the lignin and hemicellulose from Oil Palm Empty Fruit Bunch (OPEFB) at the temperature of 100°C and 1 h extraction time, which managed to retain fibrous NFC. The extracted solid and liquor at each stage were studied extensively through physiochemical analysis. The finding indicated that approximately 75.3%dwb of hemicellulose, 68.9%dwb of lignin, and 42.0%dwb of extractive were solubilized in the first SOE cycle, while the second SOE cycle resulted in 92.3%dwb, 99.6%dwb and 99.8%dwb of solubilized hemicellulose, lignin, and extractive/ash, respectively. High-quality NFC (75.52%dwb) was obtained for the final extracted solid with 76.4% crystallinity, which is near the crystallinity of standard commercial NFC. The proposed process possesses an effective synergy in producing NFC from raw OPEFB with less cellulose degradation, and most of the degraded hemicellulose and lignin are solubilized in the liquor.
Topics: Cellulose; Fruit; Oxidation-Reduction; Arecaceae; Lignin; Nanofibers; Palm Oil; Polysaccharides; Hydrogen Peroxide
PubMed: 38843202
DOI: 10.1371/journal.pone.0299312 -
Nigerian Journal of Clinical Practice May 2024Fatigue is a common problem in patients with multiple sclerosis (MS) and is one of the most serious symptoms of the disease. Although many factors play a role in the... (Randomized Controlled Trial)
Randomized Controlled Trial
BACKGROUND
Fatigue is a common problem in patients with multiple sclerosis (MS) and is one of the most serious symptoms of the disease. Although many factors play a role in the etiology of fatigue in patients with MS, it has been reported that fatigue is caused by irregular or disrupted sleep patterns.
AIM
The purpose of the study was to examine the effects of lavender oil aromatherapy on the sleep and fatigue of MS patients.
METHODS
The study was designed as a randomized controlled trial and was conducted in Turkey. The data of the study were collected using the Patient Description Form, Fatigue Severity Scale, and Pittsburgh Sleep Quality Index.
RESULTS
The mean FSS score in the experimental group was 6.86 ± 0.94 before the procedure and 3.42 ± 0.85 after the procedure, the mean PSQI score was 9.45 ± 1.23 before the procedure and 6.68 ± 2.87 after the procedure, and the difference in the mean scores between the two groups was found to be statistically significant (P < 0.001).
CONCLUSION
The results showed that aromatherapy with lavender essential oil has significant impacts on fatigue and sleep in MS patients. Lavender essential oil aromatherapy can be used by nurses as an independent nursing practice. It is an inexpensive, noninvasive, and reliable technique to manage fatigue in individuals with MS.
Topics: Humans; Oils, Volatile; Aromatherapy; Lavandula; Turkey; Female; Plant Oils; Adult; Multiple Sclerosis; Male; Fatigue; Middle Aged; Sleep; Sleep Wake Disorders; Treatment Outcome
PubMed: 38842713
DOI: 10.4103/njcp.njcp_811_23 -
Frontiers in Plant Science 2024Pear decline (PD) is one of the most devastating diseases of in Europe and North America. It is caused by the pathogen Phytoplasma pyri' and transmitted by pear...
INTRODUCTION
Pear decline (PD) is one of the most devastating diseases of in Europe and North America. It is caused by the pathogen Phytoplasma pyri' and transmitted by pear psyllids (, , and ). Identifying attractant and repellent volatile organic compounds (VOCs) could improve the development of alternative plant protection measurements like push-pull or attract-and-kill strategies against pear psyllids. Our objective was to investigate which chemical cues of the host plant could influence the host-seeking behavior of pear psyllids, and if cedarwood (CWO) and cinnamon bark (CBO) essential oils could serve as repellents.
RESULTS AND DISCUSSION
Based on the literature, the five most abundant VOCs from pear plants elicited EAG responses in both and psyllid species. In Y-olfactometer trials, single compounds were not attractive to . However, the main compound mixture was attractive to and females. CWO and CBO were repellent against , and when formulated into nanofibers (NF), both were repellent in olfactometer trials. However, CBO nanoformulation was ineffective in masking the odors of pear plants. In a field trial, attractive, repellent CWO and blank formulated NF were inserted in attractive green sticky traps. captures in traps with CWO NF were statistically lower than in traps with the attractive mixture. Nevertheless, no statistical differences in the numbers of caught specimens were observed between CWO NF and those captured in green traps baited with blank NF. Transparent traps captured fewer psyllids than green ones. In a second field study with a completed different design (push-and-count design), dispensers filled with CBO were distributed within the plantation, and attractive green sticky traps were placed around the plantation. The numbers of trapped pear psyllids increased significantly in the border of the treated plantation, showing that psyllids were repelled by the EOs in the plantation. Although further field evaluation is needed to assess and improve their effectiveness, our results show that these aromatic compounds, repellent or attractive both in nanoformulations and marking pen dispensers, offer great potential as an environmentally sustainable alternative to currently applied methods for managing pear decline vectors.
PubMed: 38841281
DOI: 10.3389/fpls.2024.1375495 -
JAMA Network Open Jun 2024
Topics: Ontario; Cannabis; Humans; Plant Oils; Product Labeling; Drug Labeling
PubMed: 38837163
DOI: 10.1001/jamanetworkopen.2024.14922