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PeerJ 2024Plastic pollution is a significant issue on the East Coast of Surabaya, emphasizing the need to develop microplastic monitoring programs. Barnacles became one of the...
BACKGROUND
Plastic pollution is a significant issue on the East Coast of Surabaya, emphasizing the need to develop microplastic monitoring programs. Barnacles became one of the potential microplastic bioindicator species on the East Coast of Surabaya. This study aimed to characterize the visual and polymers of microplastics found in barnacles and assess their potential as a bioindicator species for microplastic pollution on the East Coast of Surabaya.
METHODS
Microplastic polymer analysis was performed using ATR-FTIR.
RESULTS
A total of 196 microplastic particles were found in barnacles, water, and sediment. The size of microplastics in barnacles, water, and sediment varied, with the size in barnacles dominated by class 1 (1-10 µm), in water by class 2 (10-50 µm), and in sediments by class 3 (50-100 µm). Fragments dominated the shape of microplastics in barnacles, while water and sediment were dominated by fiber. The microplastic color in barnacles, water, and sediment was dominated by blue, and the microplastic polymer composition on barnacles, water, and sediments was dominated by cellophane (36%). was found to be predominant and identified as a potential microplastic bioindicator because it is a cosmopolitan species. Its population was found to correlate positively with cellophane (CP) accumulation. The Pearson's correlation test between barnacle length and microplastic length at a = 0.05 was inversely proportional to = - 0.411 ( < 0.05), categorized as a strong enough correlation. These findings are essential in developing monitoring programs and mitigating the impact of microplastics on the marine environment.
Topics: Animals; Microplastics; Thoracica; Environmental Monitoring; Water Pollutants, Chemical; Geologic Sediments; Spectroscopy, Fourier Transform Infrared
PubMed: 38938603
DOI: 10.7717/peerj.17548 -
Journal of Nanobiotechnology Jun 2024Efficient monitoring of glucose concentration in the human body necessitates the utilization of electrochemically active sensing materials in nonenzymatic glucose...
Controlled synthesized of ternary Cu-Co-Ni-S sulfides nanoporous network structure on carbon fiber paper: a superior catalytic electrode for highly-sensitive glucose sensing.
BACKGROUND
Efficient monitoring of glucose concentration in the human body necessitates the utilization of electrochemically active sensing materials in nonenzymatic glucose sensors. However, prevailing limitations such as intricate fabrication processes, lower sensitivity, and instability impede their practical application. Herein, ternary Cu-Co-Ni-S sulfides nanoporous network structure was synthesized on carbon fiber paper (CP) by an ultrafast, facile, and controllable technique through on-step cyclic voltammetry, serving as a superior self-supporting catalytic electrode for the high-performance glucose sensor.
RESULTS
The direct growth of free-standing Cu-Co-Ni-S on the interconnected three-dimensional (3D) network of CP boosted the active site of the composites, improved ion diffusion kinetics, and significantly promoted the electron transfer rate. The multiple oxidation states and synergistic effects among Co, Ni, Cu, and S further promoted glucose electrooxidation. The well-architected Cu-Co-Ni-S/CP presented exceptional electrocatalytic properties for glucose with satisfied linearity of a broad range from 0.3 to 16,000 μM and high sensitivity of 6829 μA mM cm. Furthermore, the novel sensor demonstrated excellent selectivity and storage stability, which could successfully evaluate the glucose levels in human serum. Notably, the novel Cu-Co-Ni-S/CP showed favorable biocompatibility, proving its potential for in vivo glucose monitoring.
CONCLUSION
The proposed 3D hierarchical morphology self-supported electrode sensor, which demonstrates appealing analysis behavior for glucose electrooxidation, holds great promise for the next generation of high-performance glucose sensors.
Topics: Copper; Electrodes; Nickel; Catalysis; Humans; Cobalt; Electrochemical Techniques; Biosensing Techniques; Sulfides; Carbon Fiber; Glucose; Nanopores; Oxidation-Reduction; Blood Glucose
PubMed: 38937768
DOI: 10.1186/s12951-024-02635-w -
BMC Oral Health Jun 2024The aim of this study was to evaluate the adhesion of Candida glabrata, Candida albicans, Candida krusei, Candida parapsilosis and Candida tropicalis yeasts to...
BACKGROUND
The aim of this study was to evaluate the adhesion of Candida glabrata, Candida albicans, Candida krusei, Candida parapsilosis and Candida tropicalis yeasts to disk-shaped resin materials produced from resin which used in the production of surgical guide with 0, 45 and 90-degrees printing orientations by Liquid Crystal Display additive manufacturing technology.
METHODS
Disk-shaped specimens were printed with surgical guide resin using the Liquid Crystal Display production technique in 3 printing orientations (0, 45 and 90-degrees). Surface roughness and contact angle values were evaluated. Real-Time PCR analysis was performed to evaluate Candida adhesion (C. glabrata, C. albicans, C. krusei, C. parapsilosis and C. tropicalis) Field emission scanning electron microscope (FESEM) images of the materials were obtained.
RESULTS
Specimens oriented at 45-degrees demonstrated higher surface roughness (P < .05) and lower contact angle values than other groups. No significant difference was found in the adhesion of C. glabrata, C. albicans, and C. parapsilosis among specimens printed at 0, 45, and 90-degrees orientations (P > .05). A higher proportion of C. krusei and C. tropicalis was found in the specimens printed at orientation degrees of 45 = 90 < 0 with statistical significance. Analyzing the adhesion of all Candida species reveals no statistical disparity among the printing orientations.
CONCLUSIONS
The surface roughness, contact angle, and adhesion of certain Candida species are affected by printing orientations. Hence, careful consideration of the printing orientation is crucial for fabricating products with desirable properties. In 45-degree production, roughness increases due to the layered production forming steps, whereas in 0-degree production, certain Candida species exhibit high adhesion due to the formation of porous structures. Consequently, considering these factors, it is advisable to opt for production at 90-degrees, while also considering other anticipated characteristics.
Topics: Surface Properties; Candida; Microscopy, Electron, Scanning; Candida glabrata; Candida tropicalis; Candida parapsilosis; Humans; Candida albicans; Materials Testing; Cell Adhesion; Real-Time Polymerase Chain Reaction; Printing, Three-Dimensional; Resins, Synthetic; Wettability
PubMed: 38937749
DOI: 10.1186/s12903-024-04505-1 -
BMC Oral Health Jun 2024Ameloblastic fibrosarcoma (AFS) is a rare malignant odontogenic tumor, commonly occurring in young adults and typically affecting the mandibular region. We report an... (Review)
Review
BACKGROUND
Ameloblastic fibrosarcoma (AFS) is a rare malignant odontogenic tumor, commonly occurring in young adults and typically affecting the mandibular region. We report an exceptionally rare and highly atypical case of AFS in an elderly female patient originating from the maxillary bone.
CASE PRESENTATION
A 66-year-old woman was admitted with a two-week history of a lump in her left upper molar. CT scans suggested a cyst in the maxillary bone. An incisional biopsy revealed a spindle cell neoplasm. MRI showed abnormalities in the left maxilla, indicating a possible tumorous lesion. The patient underwent a subtotal maxillectomy, wide tumor excision, intraoral epithelial flap transplantation, and dental extraction. Histology identified atypical tumor cells with visible mitotic figures. Immunohistochemistry showed negative for PCK and CD34 expression, but positive for Vimentin and SMA expression. The Ki-67 proliferation index ranged from 30 to 50%. These findings suggested a potentially malignant soft tissue tumor in the left maxilla, leaning towards a diagnosis of AFS. The patient received postoperative radiotherapy. There was no recurrence during the six-month follow-up.
CONCLUSION
Based on repeated pathological evidence, we report a rare case of an elderly female with AFS originating from the maxillary bone. Surgery and postoperative radiotherapy resulted in a favorable outcome.
Topics: Humans; Female; Aged; Maxillary Neoplasms; Odontogenic Tumors; Fibrosarcoma; Tomography, X-Ray Computed; Vimentin; Magnetic Resonance Imaging
PubMed: 38937725
DOI: 10.1186/s12903-024-04509-x -
Scientific Reports Jun 2024In this study, polymer-coated anisotropic inorganic nanowires dispersed in PBI matrix were introduced to construct 1D proton conducting channels within PBI. Ionic-liquid...
In this study, polymer-coated anisotropic inorganic nanowires dispersed in PBI matrix were introduced to construct 1D proton conducting channels within PBI. Ionic-liquid and solvothermal methods were used for the synthesis of ZrO and WO NWs, which were coated with PVPA and PDDA polymers to increase their proton conductivity. Our results showed that, prepared membranes have amorphous nature due to the dominating presence of PBI. SEM analysis revealed the average thickness of membrane of about 36 µm. TG/DTA analysis detected lower weight loss of WO NWs (total 2.8%) compared to ZrO NWs (18%). Proton conductivity analysis showed that, PDDA/WO NWs possess relatively 4 times higher proton conductivity (4 10 Scm) compared to PDDA/ZrO NWs (1 10 Scm) at 80 ℃. In addition, PDDA-coated WO NWs dispersed PBI membranes showed the highest fuel cell current density (1.2 A/cm) and power density (215 mW/cm) at 150 ℃ after 24 h which is nearly 2.5 times higher than pure PBI membrane. In addition, they exhibited the lowest in-situ proton resistance of about (0.47 Ω) compared with that of pure PBI membrane (0.8 Ω). Our results are introducing new concepts towards the development of thin and efficient polymer electrolyte membranes for PEM fuel cells.
PubMed: 38937620
DOI: 10.1038/s41598-024-65955-9 -
Nature Communications Jun 2024The Stöber method is a widely-used sol-gel route for synthesizing amorphous SiO colloids and conformal coatings. However, the material systems compatible with this...
The Stöber method is a widely-used sol-gel route for synthesizing amorphous SiO colloids and conformal coatings. However, the material systems compatible with this method are still limited. Herein, we have extended the approach to metal-organic frameworks (MOFs) and coordination polymers (CPs) by mimicking the Stöber method. We introduce a general synthesis route to amorphous MOFs or CPs by making use of a base-vapor diffusion method, which allows to precisely control the growth kinetics. Twenty-four different amorphous CPs colloids were successfully synthesized by selecting 12 metal ions and 17 organic ligands. Moreover, by introducing functional nanoparticles (NPs), a conformal amorphous MOFs coating with controllable thickness can be grown on NPs to form core-shell colloids. The versatility of this amorphous coating technology was demonstrated by synthesizing over 100 core-shell composites from 20 amorphous CPs shells and over 30 different NPs. Besides, various multifunctional nanostructures, such as conformal yolk-amorphous MOF shell, core@metal oxides, and core@carbon, can be obtained through one-step transformation of the core@amorphous MOFs. This work significantly enriches the Stöber method and introduces a platform, enabling the systematic design of colloids exhibiting different level of functionality and complexity.
PubMed: 38937499
DOI: 10.1038/s41467-024-49772-2 -
Nature Communications Jun 2024Atomic-scale molecular modeling and simulation are powerful tools for computational biology. However, constructing models with large, densely packed molecules, non-water...
Atomic-scale molecular modeling and simulation are powerful tools for computational biology. However, constructing models with large, densely packed molecules, non-water solvents, or with combinations of multiple biomembranes, polymers, and nanomaterials remains challenging and requires significant time and expertise. Furthermore, existing tools do not support such assemblies under the periodic boundary conditions (PBC) necessary for molecular simulation. Here, we describe Multicomponent Assembler in CHARMM-GUI that automates complex molecular assembly and simulation input preparation under the PBC. In this work, we demonstrate its versatility by preparing 6 challenging systems with varying density of large components: (1) solvated proteins, (2) solvated proteins with a pre-equilibrated membrane, (3) solvated proteins with a sheet-like nanomaterial, (4) solvated proteins with a sheet-like polymer, (5) a mixed membrane-nanomaterial system, and (6) a sheet-like polymer with gaseous solvent. Multicomponent Assembler is expected to be a unique cyberinfrastructure to study complex interactions between small molecules, biomacromolecules, polymers, and nanomaterials.
Topics: Nanostructures; Polymers; Molecular Dynamics Simulation; Proteins; Models, Molecular; Solvents; Computational Biology; Software
PubMed: 38937468
DOI: 10.1038/s41467-024-49700-4 -
Nature Communications Jun 2024Developing superporous hemostatic sponges with simultaneously enhanced permeability and mechanical properties remains challenging but highly desirable to achieve rapid...
Developing superporous hemostatic sponges with simultaneously enhanced permeability and mechanical properties remains challenging but highly desirable to achieve rapid hemostasis for non-compressible hemorrhage. Typical approaches to improve the permeability of hemostatic sponges by increasing porosity sacrifice mechanical properties and yield limited pore interconnectivity, thereby undermining the hemostatic efficacy and subsequent tissue regeneration. Herein, we propose a temperature-assisted secondary network compaction strategy following the phase separation-induced primary compaction to fabricate the superporous chitosan sponge with highly-interconnected porous structure, enhanced blood absorption rate and capacity, and fatigue resistance. The superporous chitosan sponge exhibits rapid shape recovery after absorbing blood and maintains sufficient pressure on wounds to build a robust physical barrier to greatly improve hemostatic efficiency. Furthermore, the superporous chitosan sponge outperforms commercial gauze, gelatin sponges, and chitosan powder by enhancing hemostatic efficiency, cell infiltration, vascular regeneration, and in-situ tissue regeneration in non-compressible organ injury models, respectively. We believe the proposed secondary network compaction strategy provides a simple yet effective method to fabricate superporous hemostatic sponges for diverse clinical applications.
Topics: Animals; Porosity; Chitosan; Hemostatics; Swine; Hemostasis; Permeability; Hemorrhage; Male
PubMed: 38937462
DOI: 10.1038/s41467-024-49578-2 -
Cell Death & Disease Jun 2024In eukaryotes, the nucleolus is the critical non-membranous organelle within nuclei that is responsible for ribosomal DNA (rDNA) transcription and ribosome biogenesis....
In eukaryotes, the nucleolus is the critical non-membranous organelle within nuclei that is responsible for ribosomal DNA (rDNA) transcription and ribosome biogenesis. The transcription of rDNA, a rate-limiting step for ribosome biogenesis, is tightly regulated to meet the demand for global protein synthesis in response to cell physiology, especially in neurons, which undergo rapid changes in morphology and protein composition during development and synaptic plasticity. However, it is unknown how the pre-initiation complex for rDNA transcription is efficiently assembled within the nucleolus in neurons. Here, we report that the nucleolar protein, coronin 2B, regulates rDNA transcription and maintains nucleolar function through direct interaction with upstream binding factor (UBF), an activator of RNA polymerase I transcriptional machinery. We show that coronin 2B knockdown impairs the formation of the transcription initiation complex, inhibits rDNA transcription, destroys nucleolar integrity, and ultimately induces nucleolar stress. In turn, coronin 2B-mediated nucleolar stress leads to p53 stabilization and activation, eventually resulting in neuronal apoptosis. Thus, we identified that coronin 2B coordinates with UBF to regulate rDNA transcription and maintain proper nucleolar function in neurons.
Topics: Apoptosis; Cell Nucleolus; Neurons; Animals; Pol1 Transcription Initiation Complex Proteins; Humans; DNA, Ribosomal; Microfilament Proteins; Transcription, Genetic; Tumor Suppressor Protein p53; Mice; Stress, Physiological
PubMed: 38937439
DOI: 10.1038/s41419-024-06852-x -
Environmental Pollution (Barking, Essex... Jun 2024The characterization of microplastic (MP) contamination in marine species is increasing as concerns about environmental and food safety are more and more discussed....
The characterization of microplastic (MP) contamination in marine species is increasing as concerns about environmental and food safety are more and more discussed. Here, we reported a quantitative and qualitative assessment of the contamination by anthropogenic particles (from visual sorting; AP) and MP (plastic-made) in the whole soft body or digestive tract of marine species. Four commercial species were studied, namely the Pacific oyster (Magallana gigas), the spiny spider crab (Maja sp.), the common sole (Solea solea) and seabass (Dicentrarchus labrax or punctatus). AP and MP uptake were studied over three to four seasons depending on the species. After tissues digestion, particles were extracted under a stereomicroscope and morphometric characteristics were reported. Then, polymers were identified by ATR-FTIR spectroscopy. Seasonal variations were mainly described in the Pacific oyster as AP uptake was lower in autumn and MP uptake was higher in spring. These variations may be linked to the reproduction and growth cycles of this species. Moreover, seabass ingestion was lower in autumn compared to winter. Contamination in spider crabs and soles showed either weak or no seasonal trends, both quantitatively and qualitatively. Overall, AP contamination in all studied species ranged from 1.17 ± 1.89 AP.ind (in sole) to 4.07 ± 6.69 AP.ind (in seabass) while MP contamination ranged from 0.10 ± 0.37 MP.ind (in sole) to 1.09 ± 3.06 MP.ind (in spider crab). Fibers were mostly reported in all species (at least 77.7%), along with cellulosic polymers (at least 43.7%). AP and MP uptake were detected in all species and at almost all seasons, with the only exception of the common sole during autumn. Therefore, this study emphasizes the ubiquity of AP and MP contamination in marine species and provides new knowledges about seasonal uptake by commercial species.
PubMed: 38936792
DOI: 10.1016/j.envpol.2024.124440