-
Insects Mar 2023This study was conducted to assess the bioactive value of honey samples collected from three countries. In total, 150 honey samples from Saudi Arabia (50), Libya (50),...
This study was conducted to assess the bioactive value of honey samples collected from three countries. In total, 150 honey samples from Saudi Arabia (50), Libya (50), and Egypt (50) were collected and compared, based on the results of the melissopalynological analysis, their physicochemical attributes, antioxidant and antimicrobial activities, and biochemical properties, together with their total phenolic and total flavonoid contents. Depending on the geographical origin, we observed different levels of growth suppression for six resistant bacterial strains. The pathogenic microorganisms tested in this study were and There was a strong correlation between the polyphenol and flavonoid contents, as well as significant ( < 0.05) radical scavenging activities. The melissopalynological analysis and physicochemical properties complied with the recommendation of the Gulf and Egyptian Technical Regulations on honey, as well as the Codex Alimentarius of the World Health Organization and the European Union Normative related to honey quality. It was concluded that honey from the three countries has the capacity to suppress pathogenic bacterial growth and has significant radical scavenging activities. Moreover, these findings suggest that honey may be considered as an interesting source of antimicrobial compounds and antioxidants for therapeutical and nutraceutical industries or for food manufacturers.
PubMed: 37103134
DOI: 10.3390/insects14040319 -
Molecules (Basel, Switzerland) Apr 2023Numerous natural habitats, such as soil, air, fermented foods, and human stomachs, are home to different strains. Some strains have a distinctive predominance and are...
Numerous natural habitats, such as soil, air, fermented foods, and human stomachs, are home to different strains. Some strains have a distinctive predominance and are widely recognized among other microbial communities, as a result of their varied habitation and physiologically active metabolites. The present study collected vegetable products (potato, carrot, and tomato) from local markets in Almaty, Kazakhstan. The bacterial isolates were identified using biochemical and phylogenetic analyses after culturing. Our phylogenetic analysis revealed three Gram-positive bacterial isolates BSS11, BSS17, and BSS19 showing 99% nucleotide sequence similarities with O-3, Md1-42, and Khozestan2. The crude extract was prepared from bacterial isolates to assess the antibiotic resistance potency and the antimicrobial potential against various targeted multidrug-resistant strains, including , , , , , , , , , , , , , , and . This study found that the species that were identified have the ability to produce antibiotic chemicals. Additionally, the GC-MS analysis of three bacterial extracts revealed the presence of many antibiotic substances including phenol, benzoic acid, 1,2-benzenedicarboxylic acid and bis(2-methylpropyl), methoxyphenyl-oxime, and benzaldehyde. This work sheds light on the potential of to be employed as an antimicrobial agent to target different multidrug-resistant bacterial strains. The results indicate that market vegetables may be a useful source of strains displaying a range of advantageous characteristics that can be used in the creation of biological antibiotics.
Topics: Humans; Anti-Bacterial Agents; Bacillus; Vegetables; Phylogeny; Anti-Infective Agents; Bacillus subtilis; Escherichia coli; Microbial Sensitivity Tests
PubMed: 37049972
DOI: 10.3390/molecules28073210 -
International Journal of Analytical... 2023The study showed the ability to synthesize environmentally friendly silver nanoparticles (AgNPs) using extracts from seeds and stems. Surface plasmon resonance peaks...
The study showed the ability to synthesize environmentally friendly silver nanoparticles (AgNPs) using extracts from seeds and stems. Surface plasmon resonance peaks in the UV-Vis absorption spectra of both plant extracts verified AgNP synthesis. The structural and morphological properties of the AgNPs were investigated using analytical techniques such as XRD, FTIR, TEM, and EDAX. The AgNPs have an FCC crystalline structure, according to XRD study, and their sizes range from 20 to 40 nm, according to TEM images. Based on the results, these plant extracts have been identified as suitable bioresources for AgNP production. The study also showed that both AgNPs had significant levels of antibacterial activity when tested on four different microbial strains using the agar-well diffusion method. The bacteria tested included two Gram-positive strains ( and ) and two Gram-negative strains ( and ). Furthermore, the AgNPs were found to have a significant anticancer effect on MCF-7 cell lines, suggesting that they may be useful in therapeutic applications. Overall, this research highlights the potential of the plant extracts considered as a source for synthesizing eco-friendly AgNPs with potential applications in medicine and other fields.
PubMed: 36992867
DOI: 10.1155/2023/6352503 -
Cureus Feb 2023Urinary tract infection (UTI) is one of the most commonly encountered bacterial infections. Due to the misuse or excessive use of antibiotics, the upsurge of...
BACKGROUND
Urinary tract infection (UTI) is one of the most commonly encountered bacterial infections. Due to the misuse or excessive use of antibiotics, the upsurge of multidrug-resistance cases in UTIs has now become a global threat to public health. Exploring a newer or safer treatment using green synthesized nanoparticles (NPs) is another substitute for eliminating multidrug-resistant pathogens.
METHODOLOGY
Leaf extract of was used for green synthesis of gold NPs. Synthesis of gold nanoparticles (ScAu-NPs) was achieved by optimizing various reaction parameters. These ScAu-NPs were characterized through UV-visible spectroscopy, transmission electron microscope, Fourier transform infrared spectroscopy (FTIR), and X-ray diffraction. ScAu-NPs were then processed for antibacterial activity against clinically isolated multidrug-resistant pathogens like , , and .
RESULTS
Characterization of NPs revealed that biosynthesized NPs were spherical in shape. FTIR spectroscopy showed the presence of phenolics and aromatic compounds. Biosynthesized NPs exhibit good antibacterial activity with a significant bacterial reduction seen against all bacterial isolates compared to the controls.
CONCLUSION
From the results of the present study, the formulation of biosynthesized ScAu-NPs can be utilized in drug development for eliminating infections caused by multidrug-resistant pathogens.
PubMed: 36919069
DOI: 10.7759/cureus.34830 -
TheScientificWorldJournal 2023In this study, previously isolated from Lake Mariout (Alexandria, Egypt) was cultivated in the laboratory using a medium for pharmaceutical grade , named as Amara and...
In this study, previously isolated from Lake Mariout (Alexandria, Egypt) was cultivated in the laboratory using a medium for pharmaceutical grade , named as Amara and Steinbüchel medium. Hot water extract of the Egyptian was prepared by autoclaving dried biomass in distilled water at 121°C for 15 min. This algal water extract was analyzed by GC-MS to evaluate its volatile compounds and fatty acids composition. The antimicrobial activity of phycobiliprotein extract from using phosphate buffer was evaluated against thirteen microbial strains (two Gram-positive bacteria, eight Gram-negative bacteria, one yeast, and two filamentous fungi). The major components of fatty acids in the hot extract of Egyptian were hexadecanoic acid (palmitic acid, 55.19%) and octadecanoic acid (stearic acid, 27.14%). The main constituents of its volatile compounds were acetic acid (43.33%) and oxalic acid (47.98%). The most potent antimicrobial effect of phycobiliprotein extract was obtained against two Gram-negative bacteria and , filamentous fungus , and the pathogenic yeast (all of which showed MIC values of 58.1 g/ml). and come second in their susceptibility to the phycobiliprotein extract from and and are the least in susceptibility, with MIC values of 116.2 and 232.5 g/ml, respectively, while phycobiliprotein extract has no antibacterial effect on methicillin-resistant as well as susceptible , , , and . These findings confirmed the nutritional value of Egyptian isolated from Lake Mariout and suggest the potential use of this strain as an ingredient in the cooking of some foods to increase the level of stearic acid and palmitic acid. Moreover, its effective antibacterial activities against some important and highly resistant to antibiotics bacterial pathogens in addition to its antifungal effects recommend the therapeutic use of its biomass.
Topics: Egypt; Spirulina; Fatty Acids; Lakes; Antifungal Agents; Anti-Bacterial Agents; Gram-Negative Bacteria; Yeasts; Candida albicans; Water; Stearic Acids; Palmitic Acids; Microbial Sensitivity Tests
PubMed: 36890980
DOI: 10.1155/2023/9919814 -
Food Science & Nutrition Feb 2023This study investigates the isolation and characterization of the main lactic acid bacteria responsible for fermentation of Oggtt, a dried fermented buttermilk. Five...
This study investigates the isolation and characterization of the main lactic acid bacteria responsible for fermentation of Oggtt, a dried fermented buttermilk. Five isolates with Gram-positive staining and negative catalase and oxidase activity were identified using phenotypic and genotypic methods, and their antagonistic, exopolysaccharides and organic acid production, proteolytic activity, and antioxidant capacity were assessed. The isolates are classified as Ogt_1, Ogt_2, Ogt_3, Ogt_4, and Ogt_5. All strains possessed high antagonistic activity against , and . All strains produced high levels of lactic acid (11177.3-15404.9 μg/ml), tartaric acid (2197.8-4058.5 μg/ml), and exopolysaccharides(20.86-239.9 mg/L) and possessed high proteolytic and antioxidant activity at variable manners. Overall, this study indicates the isolation of important strains from Oggtt, which could be used as starter cultures for developing functional foods.
PubMed: 36789076
DOI: 10.1002/fsn3.3140 -
African Journal of Infectious Diseases 2023This study investigated the efficacy of the essential oil (EO) of (clove) on the bacteria associated with .
BACKGROUND
This study investigated the efficacy of the essential oil (EO) of (clove) on the bacteria associated with .
MATERIALS AND METHODS
Ear swab samples were collected and bacteria isolated were identified using morphological and biochemical procedures. Essential oil was extracted from the dried flower buds using the hydro-distillation method while physicochemical and phytochemical analysis was done on the oil. Antibiotic susceptibility test and agar well diffusion was used to determine the susceptibility of the isolates to the EO. In - silico analysis was conducted to determine the drugable compound in the EO.
RESULTS
Phytochemical analysis of the oil indicated the presence of flavonoids, sterols, phenols, carbohydrates and alkaloids. Physicochemical test of the EO exhibited the presence of eugenol (80.98%) as the most abundant phytocompound. Percentage occurrence of the bacterial isolates are as follows; (31.25%), (25%), (18.25%), (3.12%), (12.5%), (3.12%) and (6.25%). Zones of inhibition were within the range of 11.5±0.71mm-23.0±2.83mm. In silico studies demonstrated that 16 out of 38 compounds identified passed the ADMET analysis. Various compounds had different binding energies, such as linalool, carvacrol for (2NOJ), gamma-eudesmol, eudesmol for (6H2L), eucalyptol, gamma-eudesmol and eudesmol for (4MCX) and (4HBL).
CONCLUSION
This study shows the potency of clove EO as an antibacterial agent and its component as potential lead molecules in drug development and design to combat multi - drug resistance.
PubMed: 36756486
DOI: 10.21010/Ajidv17i1.3 -
Virus Research Mar 2023The crystalline formation of biofilms by Proteus blocks the urine flow which often complicates the health care of catheterized patients. Bacteriophages has been...
The crystalline formation of biofilms by Proteus blocks the urine flow which often complicates the health care of catheterized patients. Bacteriophages has been highlighted as a promising tool to control biofilm-mediated bacterial infections. Here, we isolated and characterized two newly isolated lytic phages capable of infecting clinical isolates of P. mirabilis and P. vulgaris. Moreover, insights regarding the biological and molecular characterization were analysed. Both RP6 and RP7 phages showed a Proteus-genus-specific profile, administering no lytic activity against other family of Enterobacteriaceae. The optimal MOI value of the RP6 and RP7 phages were determined as 0.1 and 0.01, respectively. The one-step growth curve showed that RP6 and RP7 phages have a short latent period of 20 min and large burst size of 220-371 PFU/ML per infected host cell. Bacteria growth was reduced immediately after the phages were added, which is shown by the optical density (OD) measurement after 24 hr. Proteus phage RP6 and RP7 were found to eradicate both the planktonic and mature biofilms produced by the Proteus isolates tested. Genome sequence of Proteus phage RP6 was found to be 58,619 bp, and a G-C content of 47%. Also, Proteus phage RP7 genome size was 103,593 bp with G-C ratio of 38.45%. A total of 70 and 172 open reading frame (ORF) was encoded in RP6 and RP7 phage genomes, respectively. Interestingly, there were no tRNA encoded by Proteus phage RP6 genome even though there is a significant G-C content difference between the phage and its host. Additionally, the exhibition of highly lytic activity and absence of virulence and antibiotic-resistant genes in both Proteus RP6 and RP7 phages emphasized that this newly isolated phages are promising for potential therapeutic phages.
Topics: Humans; Bacteriophages; Sewage; Proteus; Proteus mirabilis; Genome, Viral; Biofilms; Hospitals
PubMed: 36717023
DOI: 10.1016/j.virusres.2023.199049 -
Frontiers in Microbiology 2022Diabetes mellitus is a chronic metabolic disorder that exhibited great expansion all over the world. It is becoming an epidemic disease adding a major burden to the...
Characterization, antibacterial, antioxidant, antidiabetic, and anti-inflammatory activities of green synthesized silver nanoparticles using A. G. Mill and J. A. Nyberg extract.
INTRODUCTION
Diabetes mellitus is a chronic metabolic disorder that exhibited great expansion all over the world. It is becoming an epidemic disease adding a major burden to the health care system, particularly in developing countries.
METHODS
The plant under investigation in the current study A. G. Mill and J. A. Nyberg is traditionally used in Saudi Arabia for the treatment of diabetes mellitus. The methanolic extract (200 mg/kg) of the plant and pure gallic acid (40 mg/kg), a major metabolite of the plant, as well as their silver nanoparticle formulae (AgNPs) were evaluated for their antidiabetic activity.
RESULTS AND DISCUSSION
The results showed a decrease in body fat, obesity, an improvement in lipid profiles, normalization of hyperglycemia, insulin resistance, and hyperinsulinemia, and an improvement in liver tissue structure and function. However, the results obtained from AgNPs for both extract and the pure gallic acid were better in most measured parameters. Additionally, the activity of both the crude extract of the plant and its AgNPs were evaluated against a number of gram-positive, gram-negative bacteria and fungi. Although the activity of the crude extract ranged from moderate to weak or even non-active, the AgNPs of the plant extract clearly enhanced the antimicrobial activity. AgNPs of the extract demonstrated remarkable activity, especially against the Gram-negative pathogens (MIC 2.5 μg/ml) and (MIC 5 μg/ml). Furthermore, a promising antimicrobial activity was shown against the Gram-positive pathogen (MIC 1.25 μg/ml).
PubMed: 36687608
DOI: 10.3389/fmicb.2022.1078061