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Global Change Biology Jan 2024Freshwater ecosystems are invaded by a non-random selection of taxa, among which crayfish stand out with successful examples worldwide. Species distribution models...
Freshwater ecosystems are invaded by a non-random selection of taxa, among which crayfish stand out with successful examples worldwide. Species distribution models (SDMs) have been used to detect suitable areas for invasive species and predict their potential distributions. However, these prediction exercises assume the stability of realized environmental niches, which is uncertain during invasion. Worldwide evaluations involving cosmopolitan invaders may be particularly useful but have seldom been considered. Focusing on the successful invasion history of the red swamp crayfish, Procambarus clarkii, we assessed its geographic expansion and niche trends over time. Based on global occurrences from 1854 to 2022, multiple sequential SDMs have been implemented based on a set of bioclimatic variables. The environmental suitability for each period was projected through to the next period(s) using an ensemble procedure of commonly used SDM algorithms. As the records of the species are known, it was possible to check whether the modelling projections were concordant with the observed expansion of red swamp crayfish at a global scale. This also permitted analysis of its realized niche, and its dynamics, during different expansion phases. SDM maps based on past species records showed concordance with the known crayfish distributions and yielded similar spatial patterns with outputs overperforming random combinations of cells in term of suitability. The results also reflect the stability of the species niche, which despite some expansions during the invasion process, changed little in terms of main position in functional space over time. SDMs developed in the early stages of invasion provide useful insights but also tend to underpredict the potential range compared to models that were built for later stages. Our approach can be easily transferable to other well-documented taxa and represents valuable evidence for validating the use of SDMs, considering a highly dynamic world where biogeographical barriers are often bypassed.
Topics: Animals; Ecosystem; Introduced Species; Astacoidea; Fresh Water
PubMed: 38273539
DOI: 10.1111/gcb.17059 -
International Journal of Molecular... Jan 2024Red swamp crayfish, (), is an important model crustacean organism used in many types of research. However, the effects of different doses of aminomethylphosphonic acid...
Red swamp crayfish, (), is an important model crustacean organism used in many types of research. However, the effects of different doses of aminomethylphosphonic acid (AMAP) on the transcriptome and metabolites of have not been explored. Thus, this study investigated the molecular and metabolic mechanisms activated at the different exposure dosages of AMAP in to provide new insights into the strategies of in response to the high concentrations of AMAP in the environment. In the present study, the were divided into three groups (control group; low-dosage AMAP exposure; high-dosage AMAP exposure), and hepatopancreatic tissue samples were dependently taken from the three groups. The response mechanisms at the different dosages of AMAP were investigated based on the transcriptome and metabolome data of . Differentially expressed genes and differentially abundant metabolites were identified in the distinct AMAP dosage exposure groups. The genes related to ribosome cell components were significantly up-regulated, suggesting that ribosomes play an essential role in responding to AMAP stress. The metabolite taurine, involved in the taurine and hypotaurine metabolism pathway, was significantly down-regulated. may provide feedback to counteract different dosages of AMAP via the upregulation of ribosome-related genes and multiple metabolic pathways. These key genes and metabolites play an important role in the response to AMAP stress to better prepare for survival in high AMAP concentrations.
Topics: Animals; Astacoidea; Transcriptome; Metabolome; Taurine; Organophosphonates
PubMed: 38256017
DOI: 10.3390/ijms25020943 -
Comparative Biochemistry and... Mar 2024Cherax quadricarinatus exhibit sexual dimorphism, with males outpacing females in size specification and growth rate. However, there is limited understanding of the...
Cherax quadricarinatus exhibit sexual dimorphism, with males outpacing females in size specification and growth rate. However, there is limited understanding of the molecular mechanisms underlying sex determination and sex differentiation in crustaceans. To study the differences between intersex individuals and normal individuals, this study counted the proportion of intersex individuals in the natural population, collected the proportion of 7 different phenotypes in 200 intersex individuals, and observed the differences in tissue sections. RNA-seq was used to study the different changes in the transcriptome of normal and intersex gonads. The results showed that: the percentage of intersex in the natural population was 1.5 %, and the percentage of different types of intersex ranged from 0.5 % to 22.5 %; the sections revealed that the development of normal ovaries was stagnant at the primary oocyte stage when intersex individuals with ovaries were present; We screened for pathways and genes that may be associated with gonadal development and sex, including ovarian steroid synthesis, estrogen signaling pathway, oocyte meiosis, progesterone-mediated oocyte maturation, etc. Relevant genes including tra2a, dmrta2, ccnb2, foxl2, and smad4. This study provides an important molecular basis for sex determination, sex-controlled breeding, and unisex breeding in red crayfish.
Topics: Humans; Male; Female; Animals; Astacoidea; Transcriptome; Gonads; Ovary; Phenotype
PubMed: 38246110
DOI: 10.1016/j.cbd.2024.101194 -
Scientific Reports Jan 2024Carbon sources are considered as critical input for the health and immunity of aquatic animals. The present study investigated the impact of different carbon sources on...
Carbon sources are considered as critical input for the health and immunity of aquatic animals. The present study investigated the impact of different carbon sources on water quality parameters, carbon to nitrogen (C/N) ratio and microbial community in sediments, and health responses of marron (Cherax cainii) under laboratory conditions. Following one week of acclimation, 120 marron were randomly assigned to 12 experimental tanks. There were four treatments including one untreated control and three groups with carbon addition to maintain a C/N ratio of 12 maintained in culture water. Carbon supplementation groups included corn flour (CBC12), molasses (MBC12) and wheat flour (WBC12). At the end of the 60-day trial, MBC12 resulted in the highest sediment C/N ratio, followed by CBC12. Weight gain and specific growth rate were higher in MBC12, compared to control. The protease activity in marron hepatopancreas, total haemocyte count and lysozyme activity in haemolymph were highest in MBC12. Analysis of 16S rRNA sequence data of tank sediments revealed increased bacterial alpha diversity in MBC12 and WBC12. Proteobacteria was the most abundant phylum in MBC12 (88.6%), followed by control (82.4%) and CBC12 (72.8%). Sphingobium and Novosphingobium were the most abundant genera in control and MBC12 groups, respectively. Higher Aeromonas abundance in CBC12 and Flavobacterium in WBC12 were observed. Overall results indicated that MBC12 led to improved water quality, retaining high C/N ratio and enriched the bacterial populations in sediments resulting in improved growth and immune performance of marron.
Topics: Animals; Astacoidea; RNA, Ribosomal, 16S; Flour; Triticum; Bacteria; Geologic Sediments; Carbon
PubMed: 38228662
DOI: 10.1038/s41598-024-51585-8 -
Zootaxa Aug 2023Cherax woworae n. sp. is a moderately-sized burrowing freshwater crayfish endemic to the Teminabuan District, South Sorong Regency, Southwest Papua Province, Indonesia....
Cherax woworae n. sp. is a moderately-sized burrowing freshwater crayfish endemic to the Teminabuan District, South Sorong Regency, Southwest Papua Province, Indonesia. This species is one of many freshwater crayfish species from the region that are popular ornamental animals. In the pet trade, it is commonly known as the Blue Moon Crayfish or Blue Kong Crayfish. Cherax woworae is genetically and morphologically similar to Cherax gherardii Patoka, Blha & Kouba, 2015, but both species may be easily distinguished morphologically and by their substantial genetic divergence.
Topics: Animals; Astacoidea; Indonesia; Decapoda; Fresh Water
PubMed: 38220891
DOI: 10.11646/zootaxa.5325.4.8 -
Food Chemistry May 2024The purpose of this study was to compare the influences of gamma-poly glutamic acid (γ-PGA) (1, 2, 3, and 4 %) to see which could outperform conventional...
The purpose of this study was to compare the influences of gamma-poly glutamic acid (γ-PGA) (1, 2, 3, and 4 %) to see which could outperform conventional cryoprotectant mixture (4 % sorbitol + 4 % sucrose) on cooked crayfish properties, such as physicochemical, textural qualities, oxidation reaction, water distributions, and microstructure integrity, during different freeze-thaw cycles. Crayfish quality characteristics improved significantly as γ-PGA concentration increased compared to control samples.Adding γ-PGA 4 % reduced the carbonyl content from 4.20 to 3.00 nmol/ mg protein during fluctuation-1 (F1), and from 4.15 to 2.80 nmol/ mg protein during fluctuation-2 (F2) compared to control samples. Furthermore, it increased the total sulfhydryl content from 4.15 and 4.76 to 6.19 and 6.47 mol/10 g protein during F1 and F2 and after five freeze-thaw cycles (FTC). This suggests that this concentration was more effective at controlling protein changes than other concentrations. γ-PGA generally enhanced the water-holding capacity by preventing protein denaturation and limiting ice crystal recrystallization. As a result, microstructure stability was evident, texture degradation was avoided, and the crayfish's color was preserved.
Topics: Animals; Astacoidea; Temperature; Freezing; Water; Polyglutamic Acid
PubMed: 38219359
DOI: 10.1016/j.foodchem.2023.138258 -
Journal of Environmental Management Feb 2024It has been proposed that biomonitoring may benefit from the use of metabolomics (the study of all small molecules in an organism) to detect sub-lethal organism stress...
It has been proposed that biomonitoring may benefit from the use of metabolomics (the study of all small molecules in an organism) to detect sub-lethal organism stress through changes in the metabolite profile (i.e., the metabolome). However, to integrate the metabolome into biomonitoring programs the amount of natural variability among and within populations of indicator taxa must be established prior to generating a reference condition. This study determined variation in the metabolome among ecoregion and stream of origin in the northern crayfish (Faxonius virilis) and if that variation inhibited detection of stressor effects at sites exposed to human activities. We collected crayfish from seven minimally disturbed streams (i.e., reference streams), distributed across three level II ecoregions in central Canada and compared their metabolomes. We found ecoregion and stream origin were poor predictors of crayfish metabolomes. This result suggests crayfish metabolomes were similar, despite differing environmental conditions. Metabolomes of crayfish collected from three stream sites exposed to agricultural activity and municipal wastewater (i.e., test sites) were then compared to the crayfish metabolomes from the seven reference streams. Findings showed that crayfish metabolomes from test sites were strongly differentiated from those at all reference sites. The consistency in the northern crayfish metabolome at the studied reference streams indicates that a single reference condition may effectively detect impacts of human activities across the sampled ecoregions.
Topics: Animals; Humans; Biological Monitoring; Astacoidea; Environmental Monitoring; Metabolome; Metabolomics
PubMed: 38211428
DOI: 10.1016/j.jenvman.2024.120076 -
Applied Microbiology and Biotechnology Dec 2024The intestinal microbiota interacts with the host and plays an important role in the immune response, digestive physiology, and regulation of body functions. In...
The intestinal microbiota interacts with the host and plays an important role in the immune response, digestive physiology, and regulation of body functions. In addition, it is also well documented that the intestinal microbiota of aquatic animals are closely related to their growth rate. However, whether it resulted in different sizes of crayfish in the rice-crayfish coculture model remained vague. Here, we analyzed the intestinal microbiota characteristics of crayfish of three sizes in the same typical rice-crayfish coculture field by high-throughput sequencing technology combined with quantitative real-time polymerase chain reaction (qRT-PCR) and enzyme activity, investigating the relationship between intestinal microbiota in crayfish and water and sediments. The results showed that the dominant intestinal microbiota of crayfish was significantly different between the large size group (BS), normal size group (NS), and small size group (SS), where Bacteroides and Candidatus_Bacilloplasma contributed to the growth of crayfish by facilitating food digestion through cellulolysis, which might be one of the potential factors affecting the difference in sizes. Follow-up experiments confirmed that the activity of lipase (LPS) and protease was higher in BS, and the relative expression of development-related genes, including alpha-amylase (α-AMY), myocyte-specific enhancer factor 2a (MEF2a), glutathione reductase (GR), chitinase (CHI), and ecdysone receptor (EcR), in BS was significantly higher than that in SS. These findings revealed the intestinal microbiota characteristics of crayfish of different sizes and their potential impact on growth, which is valuable for managing and manipulating the intestinal microbiota in crayfish to achieve high productivity in practice. KEY POINTS: • Significant differences in the dominant microflora of BS, NS, and SS in crayfish. • Cellulolysis might be a potential factor affecting different sizes in crayfish. • Adding Bacteroides and Candidatus_Bacilloplasma helped the growth of crayfish.
Topics: Animals; Gastrointestinal Microbiome; Astacoidea; Microbiota; Seafood; Bacteroides; Oryza
PubMed: 38204126
DOI: 10.1007/s00253-023-12914-5 -
Journal of Virology Feb 2024Autophagy generally functions as a cellular surveillance mechanism to combat invading viruses, but viruses have evolved various strategies to block autophagic...
Autophagy generally functions as a cellular surveillance mechanism to combat invading viruses, but viruses have evolved various strategies to block autophagic degradation and even subvert it to promote viral propagation. White spot syndrome virus (WSSV) is the most highly pathogenic crustacean virus, but little is currently known about whether crustacean viruses such as WSSV can subvert autophagic degradation for escape. Here, we show that even though WSSV proliferation triggers the accumulation of autophagosomes, autophagic degradation is blocked in the crustacean species red claw crayfish. Interestingly, the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complex including SNAP29, VAMP7, and the novel autophagosome SNARE protein Syx12 is required for autophagic flux to restrict WSSV replication, as revealed by gene silencing experiments. Simultaneously, the expressed WSSV tegument protein VP26, which likely localizes on autophagic membrane mediated by its transmembrane region, binds the Qb-SNARE domain of SNAP29 to competitively inhibit the binding of Syx12-Qa-SNARE with SNAP29-Qb-SNARE; this in turn disrupts the assembly of the Syx12-SNAP29-VAMP7 SNARE complex, which is indispensable for the proposed fusion of autophagosomes and lysosomes. Consequently, the autophagic degradation of WSSV is likely suppressed by the expressed VP26 protein in crayfish, thus probably protecting WSSV components from degradation via the autophagosome-lysosome pathway, resulting in evasion by WSSV. Collectively, these findings highlight how a DNA virus can subvert autophagic degradation by impairing the assembly of the SNARE complex to achieve evasion, paving the way for understanding host-DNA virus interactions from an evolutionary point of view, from crustaceans to mammals.IMPORTANCEWhite spot syndrome virus (WSSV) is one of the largest animal DNA viruses in terms of its genome size and has caused huge economic losses in the farming of crustaceans such as shrimp and crayfish. Detailed knowledge of WSSV-host interactions is still lacking, particularly regarding viral escape from host immune clearance. Intriguingly, we found that the presence of WSSV-VP26 might inhibit the autophagic degradation of WSSV in the crustacean species red claw crayfish. Importantly, this study is the first to show that viral protein VP26 functions as a core factor to benefit WSSV escape by disrupting the assembly of the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complex, which is necessary for the proposed fusion of autophagosomes with lysosomes for subsequent degradation. These findings highlight a novel mechanism of DNA virus evasion by blocking SNARE complex assembly and identify viral VP26 as a key candidate for anti-WSSV targeting.
Topics: Animals; Astacoidea; Autophagosomes; Autophagy; Qb-SNARE Proteins; SNARE Proteins; Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins; White spot syndrome virus 1
PubMed: 38189252
DOI: 10.1128/jvi.01408-23 -
Environmental Pollution (Barking, Essex... Mar 2024Isoprothiolane (IPT) and tricyclazole (TCZ) are widely used in rice farming and recently in combined rice-fish farming. However, co-cultured animals are affected by...
Risk assessment of pesticide compounds: IPT and TCZ cause hepatotoxicity, activate stress pathway and affect the composition of intestinal flora in red swamp crayfish (Procambarusclarkii).
Isoprothiolane (IPT) and tricyclazole (TCZ) are widely used in rice farming and recently in combined rice-fish farming. However, co-cultured animals are affected by these pesticides. To investigate the organismal effects and toxicity of pesticides, crayfish were exposed to 0, 1, 10, or 100 ppt TCZ or IPT for 7 days. Pesticide bioaccumulation, survival rate, metabolic parameters, structure of intestinal flora, and antioxidant-, apoptosis-, and HSP-related gene expression were determined. Pesticide exposure caused bioaccumulation of IPT or TCZ in the hepatopancreas and muscles of crayfish; however, IPT bioaccumulation was higher than that of TCZ. Both groups showed significant changes in hepatopancreatic serum biochemical parameters. Mitochondrial damage and chromosomal agglutination were observed in hepatopancreatic cells exposed to 100 ppt IPT or TCZ. IPT induced more significant changes in serum biochemical parameters than TCZ. The results of intestinal flora showed that Vibro, Flavobacterium, Anaerorhabdus and Shewanella may have potential for use as a bacterial marker of TCZ and IPT. Antioxidant-, apoptosis-, and HSP-related gene expression was disrupted by pesticide exposure, and was more seriously affected by IPT. The results suggest that IPT or TCZ induce hepatopancreatic cell toxicity; however, IPT or TCZ content in dietary crayfish exposed to 1 ppt was below the food safety residue standard. The data indicated that IPT exposure may be more toxic than TCZ exposure in hepatopancreas and intestines and toxicity of organism are alleviated by activating the pathway of stress-response, providing an understanding of pesticide compounds in rice-fish farming and food safety.
Topics: Animals; Antioxidants; Pesticides; Gastrointestinal Microbiome; Astacoidea; Risk Assessment; Chemical and Drug Induced Liver Injury; Thiazoles; Thiophenes
PubMed: 38185353
DOI: 10.1016/j.envpol.2024.123315