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Macromolecular Rapid Communications May 2024Molecular motor amphiphiles have already been widely attempted for dynamic nanosystems across multiple length-scale for developments of small functional materials,...
Molecular motor amphiphiles have already been widely attempted for dynamic nanosystems across multiple length-scale for developments of small functional materials, including controlling macroscopic foam properties, amplifying motion as artificial molecular muscles, and serving as extracellular matrix mimicking cell scaffolds. However, limiting examples of bola-type molecular motor amphiphiles are considered for constructing macroscopic biomaterials. Herein, this work presents the designed two second generation molecular motor amphiphiles, motor bola-amphiphiles (MBAs). Aside from the photoinduced motor rotation of MBAs achieved in both organic and aqueous media, the rate of recovering thermal helix inversion step can be controlled by the rotor part with different steric hindrances. Dynamic assembled structures of MBAs are observed under (cryo)-transmission electron microscopy (TEM). This dynamicity assists MBAs in further assembling as macroscopic soft scaffolds by applying a shear-flow method. Upon photoirradiation, the phototropic bending function of MBA scaffolds is observed, demonstrating the amplification of molecular motion into macroscopic phototropic bending functions at the macroscopic length-scale. Since MBAs are confirmed with low cytotoxicity, human bone marrow-derived mesenchymal stem cells (hBM-MSCs) can grow on the surface of MBA scaffolds. These results clearly demonstrate the concept of designing MBAs for developing photoresponsive dynamic functional materials to create new-generation soft robotic systems and cell-material interfaces.
PubMed: 38805189
DOI: 10.1002/marc.202400261 -
Physical Review Letters May 2024We demonstrate the emergence of nonreciprocal superradiant phase transitions and novel multicriticality in a cavity quantum electrodynamics system, where a two-level...
We demonstrate the emergence of nonreciprocal superradiant phase transitions and novel multicriticality in a cavity quantum electrodynamics system, where a two-level atom interacts with two counterpropagating modes of a whispering-gallery-mode microcavity. The cavity rotates at a certain angular velocity and is directionally squeezed by a unidirectional parametric pumping χ^{(2)} nonlinearity. The combination of cavity rotation and directional squeezing leads to nonreciprocal first- and second-order superradiant phase transitions. These transitions do not require ultrastrong atom-field couplings and can be easily controlled by the external pump field. Through a full quantum description of the system Hamiltonian, we identify two types of multicritical points in the phase diagram, both of which exhibit controllable nonreciprocity. These results open a new door for all-optical manipulation of superradiant transitions and multicritical behaviors in light-matter systems, with potential applications in engineering various integrated nonreciprocal quantum devices.
PubMed: 38804940
DOI: 10.1103/PhysRevLett.132.193602 -
Physical Review Letters May 2024We present the operating principle and the first observing run of a novel kind of direct detector for axions and axionlike particles in the galactic halo. Sensitive to...
We present the operating principle and the first observing run of a novel kind of direct detector for axions and axionlike particles in the galactic halo. Sensitive to the polarisation rotation of linearly polarised laser light induced by an axion field, our experiment is the first detector of its kind collecting scientific data. We discuss our peak sensitivity of 1.51×10^{-10} GeV^{-1} (95% confidence level) to the axion-photon coupling strength in the axion mass range of 1.97-2.01 neV which is, for instance, motivated by supersymmetric grand-unified theories. We also report on effects that arise in our high-finesse in-vacuum cavity at an unprecedented optical continuous-wave intensity of 4.7 MW/cm^{2}. Our detector already belongs to the most sensitive direct searches within its measurement band, and our results pave the way towards surpassing the current sensitivity limits even of astrophysical observations in the mass range from 10^{-8} down to 10^{-16} eV via quantum-enhanced laser interferometry, especially with the potential of scaling our detector up to kilometer length.
PubMed: 38804919
DOI: 10.1103/PhysRevLett.132.191002 -
Analytical Methods : Advancing Methods... Jun 2024Fluorescent labeling is a widely used method for protein detection and fluorescence imaging. A solvatochromic and fluorogenic molecular rotor DASPBCl was developed for...
Fluorescent labeling is a widely used method for protein detection and fluorescence imaging. A solvatochromic and fluorogenic molecular rotor DASPBCl was developed for covalent protein labeling in solution and SDS-PAGE, and also for stable mitochondria labeling and fluorescence imaging. The dye DASPBCl consisted of a 4-(,-dimethylamino)phenyl moiety as the electron donor and a positively charged -benzylpyridinium moiety as the electron acceptor. A benzyl chloride group was introduced into the pyridine moiety for covalent labeling of thiol in proteins. When the fluorescent dye DASPBCl is covalently labeled to the thiol of proteins, significantly enhanced fluorescence was obtained, which is attributed to the polarity sensitivity caused solvatochromic effect from the hydrophobic protein structure and the viscosity sensitivity caused fluorogenic effect from the restriction of single bond rotation. DASPBCl exhibits high sensitivity and good linear response for protein detection in SDS-PAGE analysis with both the pre-staining method and post-staining method. DASPBCl was also successfully used for covalently protein-anchored fluorescence imaging of mitochondria in living cells.
Topics: Fluorescent Dyes; Mitochondria; Humans; Electrophoresis, Polyacrylamide Gel; Sulfhydryl Compounds; Optical Imaging; HeLa Cells; Staining and Labeling; Proteins
PubMed: 38804857
DOI: 10.1039/d4ay00376d -
Phytochemistry Aug 2024Fungi from the genus Aspergillus are important resources for the discovery of bioactive agents. This investigation characterized the isolation, structural elucidation,...
Fungi from the genus Aspergillus are important resources for the discovery of bioactive agents. This investigation characterized the isolation, structural elucidation, and antimicrobial evaluation of 46 metabolites produced by the marine-derived fungus Aspergillus sp. ZZ1861 in rice solid and potato dextrose broth liquid media. The structures of these isolated compounds were determined based on their HRESIMS data, NMR spectral analyses, and data from ECD, NMR, and optical rotation calculations. Emericelactones F and G, 20R,25S-preshamixanthone, 20R,25R-preshamixanthone, phthalimidinic acid A, phthalimidinic acid B, aspergilol G, and 2-hydroxyemodic amide are eight previously undescribed compounds and (S)-2-(5-hydroxymethyl-2-formylpyrrol-1-yl) propionic acid lactone is reported from a natural resource for the first time. It is also the first report of the configurations of 25S-O-methylarugosin A, 25R-O-methylarugosin A, 5R-(+)-9-hydroxymicroperfuranone, and 5R-(+)-microperfuranone. Phthalimidinic acid A, phthalimidinic acid B, aspergilol G, and 2-hydroxyemodic amide have antifungal activity against Candida albicans with MIC values of 1.56, 3.12, 1.56, and 12.5 μg/mL, respectively, 20R,25S-preshamixanthone (MIC 25 μg/mL) shows antibacterial activity against Escherichia coli, and 20R,25R-preshamixanthone exhibits antimicrobial activity against all three tested pathogens of methicillin-resistant Staphylococcus aureus, E. coli, and C. albicans with MIC values of 50, 25, 25 μg/mL, respectively.
Topics: Aspergillus; Microbial Sensitivity Tests; Anti-Bacterial Agents; Molecular Structure; Antifungal Agents; Structure-Activity Relationship; Candida albicans
PubMed: 38797256
DOI: 10.1016/j.phytochem.2024.114164 -
Biosensors & Bioelectronics Sep 2024The low abundance, heterogeneous expression, and temporal changes of miRNA in different cellular locations pose significant challenges for both the detection sensitivity...
The low abundance, heterogeneous expression, and temporal changes of miRNA in different cellular locations pose significant challenges for both the detection sensitivity of miRNA liquid biopsy and intracellular imaging. In this work, we report an intelligently assembled biosensor based on catalytic hairpin assembly (CHA) and aggregation-induced emission (AIE), named as catalytic hairpin aggregation-induced emission (CHAIE), for the ultrasensitive detection and intracellular imaging of miRNA-155. To achieve such goal, tetraphenylethylene-N (TPE-N) is used as AIE luminogen (AIEgen), while graphene oxide is introduced to quench the fluorescence. When the target miRNA is present, CHA reaction is triggered, causing the AIEgen to self-assemble with the hairpin DNA. This will restrict the intramolecular rotation of the AIEgen and produce a strong AIE fluorescence. Interestingly, CHAIE does not require any enzyme or expensive thermal cycling equipment, and therefore provides a rapid detection. Under optimal conditions, the proposed biosensor can determine miRNA in the concentration range from 2 pM to 200 nM within 30 min, with the detection limit of 0.42 pM. The proposed CHAIE biosensor in this work offers a low background signal and high sensitivity, making it applicable for highly precise spatiotemporal imaging of target miRNA in living cells.
Topics: Graphite; MicroRNAs; Biosensing Techniques; Humans; Nanocomposites; Fluorescent Dyes; Limit of Detection; Stilbenes; Catalysis; Optical Imaging; Spectrometry, Fluorescence; Fluorescence
PubMed: 38797033
DOI: 10.1016/j.bios.2024.116416 -
Sensors (Basel, Switzerland) May 2024Transient Luminous Events (TLEs) are short-lived, upper-atmospheric optical phenomena associated with thunderstorms. Their rapid and random occurrence makes manual...
Transient Luminous Events (TLEs) are short-lived, upper-atmospheric optical phenomena associated with thunderstorms. Their rapid and random occurrence makes manual classification laborious and time-consuming. This study presents an effective approach to automating the classification of TLEs using state-of-the-art Convolutional Neural Networks (CNNs) and a Vision Transformer (ViT). The ViT architecture and four different CNN architectures, namely, ResNet50, ResNet18, GoogLeNet, and SqueezeNet, are employed and their performance is evaluated based on their accuracy and execution time. The models are trained on a dataset that was augmented using rotation, translation, and flipping techniques to increase its size and diversity. Additionally, the images are preprocessed using bilateral filtering to enhance their quality. The results show high classification accuracy across all models, with ResNet50 achieving the highest accuracy. However, a trade-off is observed between accuracy and execution time, which should be considered based on the specific requirements of the task. This study demonstrates the feasibility and effectiveness of using transfer learning and pre-trained CNNs for the automated classification of TLEs.
PubMed: 38794062
DOI: 10.3390/s24103208 -
Science Bulletin May 2024The Bloch band theory and Brillouin zone (BZ) that characterize wave-like behaviors in periodic mediums are two cornerstones of contemporary physics, ranging from...
The Bloch band theory and Brillouin zone (BZ) that characterize wave-like behaviors in periodic mediums are two cornerstones of contemporary physics, ranging from condensed matter to topological physics. Recent theoretical breakthrough revealed that, under the projective symmetry algebra enforced by artificial gauge fields, the usual two-dimensional (2D) BZ (orientable Brillouin two-torus) can be fundamentally modified to a non-orientable Brillouin Klein bottle with radically distinct manifold topology. However, the physical consequence of artificial gauge fields on the more general three-dimensional (3D) BZ (orientable Brillouin three-torus) was so far missing. Here, we theoretically discovered and experimentally observed that the fundamental domain and topology of the usual 3D BZ can be reduced to a non-orientable Brillouin Klein space or an orientable Brillouin half-turn space in a 3D acoustic crystal with artificial gauge fields. We experimentally identify peculiar 3D momentum-space non-symmorphic screw rotation and glide reflection symmetries in the measured band structures. Moreover, we experimentally demonstrate a novel stacked weak Klein bottle insulator featuring a nonzero Z topological invariant and self-collimated topological surface states at two opposite surfaces related by a nonlocal twist, radically distinct from all previous 3D topological insulators. Our discovery not only fundamentally modifies the fundamental domain and topology of 3D BZ, but also opens the door towards a wealth of previously overlooked momentum-space multidimensional manifold topologies and novel gauge-symmetry-enriched topological physics and robust acoustic wave manipulations beyond the existing paradigms.
PubMed: 38782659
DOI: 10.1016/j.scib.2024.05.003 -
Nature Communications May 2024Van-der-Waals magnetic materials can be exfoliated to realize ultrathin sheets or interfaces with highly controllable optical or spintronics responses. In majority,...
Van-der-Waals magnetic materials can be exfoliated to realize ultrathin sheets or interfaces with highly controllable optical or spintronics responses. In majority, these are collinear ferro-, ferri-, or antiferromagnets, with a particular scarcity of lattice-incommensurate helimagnets of defined left- or right-handed rotation sense, or helicity. Here, we report polarized neutron scattering experiments on DyTe, whose layered structure has highly metallic tellurium layers separated by double-slabs of dysprosium square nets. We reveal cycloidal (conical) magnetic textures, with coupled commensurate and incommensurate order parameters, and probe the evolution of this ground state in a magnetic field. The observations are well explained by a one-dimensional spin model, with an off-diagonal on-site term that is spatially modulated by DyTe's unconventional charge density wave (CDW) order. The CDW-driven term couples to antiferromagnetism, or to the net magnetization in an applied magnetic field, and creates a complex magnetic phase diagram indicative of competing interactions in this easily cleavable van-der-Waals helimagnet.
PubMed: 38769112
DOI: 10.1038/s41467-024-47127-5 -
Faraday Discussions May 2024Collisions of optically centrifuged CO molecules with = 244-282 ( = 22 800-30 300 cm) are investigated with high-resolution transient IR absorption spectroscopy to...
Collisions of optically centrifuged CO molecules with = 244-282 ( = 22 800-30 300 cm) are investigated with high-resolution transient IR absorption spectroscopy to reveal collisional and orientational phenomena of molecules with hyper-thermal rotational energies. The optical centrifuge is a non-resonant optical excitation technique that uses ultrafast, 800 nm chirped pulses to drive molecules to extreme rotational states through sequential Raman transitions. The extent of rotational excitation is controlled by tuning the optical bandwidth of the excitation pulses. Frequencies of 30 R-branch fundamental IR probe transitions are measured for the = 186-282 states of CO, expanding beyond previously reported IR transitions up to = 128. The optically centrifuged molecules have oriented angular momentum and unidirectional rotation. Polarization-sensitive transient IR absorption of individual rotational states of optically centrifuged molecules and their collision products reveals information about collisional energy transfer, relaxation kinetics, and dynamics of rotation-to-translation energy transfer. The transient IR probe also measures the extent of polarization anisotropy. Rotational energy transfer for lower energy molecules is discussed in terms of statistical models and a comparison highlights the role of increasing energy gap with and angular momentum of the optically centrifuged molecules.
PubMed: 38766993
DOI: 10.1039/d3fd00179b