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IET Nanobiotechnology 2024The massive growth of various microorganisms on the orthodontic bracket can form plaques and cause diseases. A novel amine-terminated hyperbranched...
The massive growth of various microorganisms on the orthodontic bracket can form plaques and cause diseases. A novel amine-terminated hyperbranched zirconium-polysiloxane (HPZP) antimicrobial coating was developed for an orthodontic stainless steel tank (SST). After synthesizing HPZP and HPZP-Ag coatings, their structures were characterized by nuclear magnetic resonance spectroscopy, scanning electron microscopy, thickness measurement, contact angle detection, mechanical stability testing, and corrosion testing. The cell toxicity of the two coatings to human gingival fibroblasts (hGFs) and human oral keratinocytes (hOKs) was detected by cell counting kit eight assays, and SST, HPZP@SST, and HPZP-Ag@SST were cocultured with , , and for 24 hr to detect the antibacterial properties of the coatings, respectively. The results show that the coatings are about 10 m, and the water contact angle of HPZP coating is significantly higher than that of HPZP-Ag coating ( < 0.01). Both coatings can be uniformly and densely distributed on SST and have good mechanical stability and corrosion resistance. The cell counting test showed that HPZP coating and HPZP-Ag coating were less toxic to cells compared with SST, and the toxicity of HPZP-Ag coating was greater than that of HPZP coating, with the cell survival rate greater than 80% after 72 hr cocultured with hGFs and hOKs. The antibacterial test showed that the number of bacteria on the surface of different materials was ranked from small to large: HPZP@SST < HPZP-Ag@SST < SST and 800 g/mL HPZP@SST showed a better bactericidal ability than 400 g/mL after cocultured , , and , respectively (all < 0.05). The results showed that HPZP coating had a better effect than HPZP-Ag coating, with effective antibacterial and biocompatible properties, which had the potential to be applied in orthodontic process management.
Topics: Stainless Steel; Coated Materials, Biocompatible; Humans; Anti-Bacterial Agents; Orthodontic Brackets; Zirconium; Siloxanes; Fibroblasts; Materials Testing; Amines; Staphylococcus aureus; Surface Properties; Escherichia coli; Keratinocytes; Cell Survival; Gingiva
PubMed: 38863970
DOI: 10.1049/2024/4391833 -
ACS Applied Materials & Interfaces Jun 2024Understanding of the interactions between macrophages and multifunctional nanoparticles is important for development of novel macrophage-based immunotherapies. Here, we...
Understanding of the interactions between macrophages and multifunctional nanoparticles is important for development of novel macrophage-based immunotherapies. Here, we investigated the effects of fluorescent thiol-organosilica particle size and surface properties on cell-particle interactions, including mitochondrial activity, using the mouse macrophage cell line J774A.1. Three different sizes of thiol-organosilica particles (150, 400, and 680 nm in diameter) containing fluorescein (OS/F150, OS/F400, and OS/F680) and particles surface functionalized with polyethylenimine (PEI) (OS/F150PEI, OS/F400PEI, and OS/F680PEI) were prepared. Flow cytometric analysis, time-lapse imaging, and single-cell analysis of particle uptake and mitochondrial activity of J774A.1 cells demonstrated variations in uptake and kinetics depending on the particle size and surface as well as on each individual cell. Cells treated with OS/F150 and OS/F150PEI showed higher uptake and mitochondrial activity than those treated with other particles. The interaction between endosomes and mitochondria was observed using 3D fluorescent imaging and was characterized by the involvement of iron transport into mitochondria by iron-containing proteins adsorbed on the particle surface. Scanning electron microscopy of the cells treated with the particles revealed alterations in cell membrane morphology, depending on particle size and surface. We performed correlative light and electron microscopy combined with time-lapse and 3D imaging to develop an integrated correlation analysis of particle uptake, mitochondrial activity, and cell membrane morphology in single macrophages. These cell-specific characteristics of macrophages against functional particles and their evaluation methods are crucial for understanding the immunological functions of individual macrophages and developing novel immunotherapies.
Topics: Mice; Animals; Mitochondria; Particle Size; Surface Properties; Macrophages; Organosilicon Compounds; Cell Line; Polyethyleneimine; Nanoparticles
PubMed: 38857433
DOI: 10.1021/acsami.4c06455 -
Biosensors & Bioelectronics Oct 2024Multiplex detection of low-abundance protein biomarkers in biofluids can contribute to diverse biomedical fields such as early diagnosis and precision medicine. However,...
Multiplex detection of low-abundance protein biomarkers in biofluids can contribute to diverse biomedical fields such as early diagnosis and precision medicine. However, conventional techniques such as digital ELISA, microarray, and hydrogel-based assay still face limitations in terms of efficient protein detection due to issues with multiplexing capability, sensitivity, or complicated assay procedures. In this study, we present the degassed micromold-based particle isolation technique for highly sensitive and multiplex immunoassay with enzymatic signal amplification. Using degassing treatment of nanoporous polydimethylsiloxane (PDMS) micromold, the encoded particles are isolated in the mold within 5 min absorbing trapped air bubbles into the mold by air suction capability. Through 10 min of signal amplification in the isolated spaces by fluorogenic substrate and horseradish peroxidase labeled in the particle, the assay signal is amplified with one order of magnitude compared to that of the standard hydrogel-based assay. Using the signal amplification assay, vascular endothelial growth factor (VEGF) and chorionic gonadotropin beta (CG beta), the preeclampsia-related protein biomarkers, are quantitatively detected with a limit of detection (LoD) of 249 fg/mL and 476 fg/mL in phosphate buffer saline. The multiplex immunoassay is conducted to validate negligible non-specific detection signals and robust recovery rates in the multiplex assay. Finally, the VEGF and CG beta in real urine samples are simultaneously and quantitatively detected by the developed assay. Given the high sensitivity, multiplexing capability, and process simplicity, the presented particle isolation-based signal amplification assay holds significant potential in biomedical and proteomic fields.
Topics: Humans; Biosensing Techniques; Limit of Detection; Immunoassay; Vascular Endothelial Growth Factor A; Dimethylpolysiloxanes; Chorionic Gonadotropin, beta Subunit, Human; Biomarkers; Female; Pregnancy; Equipment Design
PubMed: 38850735
DOI: 10.1016/j.bios.2024.116465 -
ACS Applied Materials & Interfaces Jun 2024Wearable smart textile sensors for monitoring vital signs are fast, noninvasive, and highly desirable for personalized health management to diagnose health anomalies...
Wearable smart textile sensors for monitoring vital signs are fast, noninvasive, and highly desirable for personalized health management to diagnose health anomalies such as cardiovascular diseases and respiratory dysfunction. Traditional biosignal sensors, with power consumption issues, constrain the use of wearable medical devices. This study introduces an autonomous triboelectric smart textile sensor (AUTS) made of reduced graphene oxide/manganese dioxide/polydimethylsiloxane (RGO-M-PDMS) and polytetrafluoroethylene (TEFLON)-knitted silver electrode, offering promise for vital sign monitoring with self-powering, flexibility, and wearability. The sensor exhibits impressive output performance, with a sensitivity of 7.8 nA/kPa, response time of ≈40 ms, good stability of >15,000 cycles, stretchability of up to 40%, and machine washability of >20 washes. The AUTS has been integrated to the TriBreath respiratory belt for monitoring respiratory signals and pulse strap for pulse signals concurrently at different body pulse points. These sensors wirelessly transmitted the acquired biosignals to a smartphone, demonstrating the potential of a self-powered and real-time vital sign monitoring system.
Topics: Textiles; Graphite; Wearable Electronic Devices; Humans; Monitoring, Physiologic; Oxides; Vital Signs; Manganese Compounds; Dimethylpolysiloxanes; Polytetrafluoroethylene; Electrodes; Silver
PubMed: 38847366
DOI: 10.1021/acsami.4c04689 -
Journal of the Royal Society, Interface Jun 2024In the area of surgical applications, understanding the interaction between medical device materials and tissue is important since this interaction may cause...
In the area of surgical applications, understanding the interaction between medical device materials and tissue is important since this interaction may cause complications. The interaction often consists of a cell monolayer touching the medical device that can be mimicked . Prominent examples of this are contact lenses, where epithelial cells interact with the contact lens, or stents and catheters, which are in contact with endothelial cells. To investigate those interactions, in previous studies, expensive microtribometers were used to avoid pressures in the contact area far beyond physiologically relevant levels. Here, we aim to present a new methodology that is cost- and time-efficient, more accessible than those used previously and allows for the application of more realistic pressures, while permitting a quantification of the damage caused to the monolayer. For this, a soft polydimethylsiloxane is employed that better mimics the mechanical properties of blood vessels than materials used in other studies. Furthermore, a technique to account for misalignments within the experiment set-up is presented. This is carried out using the raw spatial and force data recorded by the tribometer and adjusting for misalignments. The methodology is demonstrated using an endothelial cell (human umbilical vein endothelial cells) monolayer.
Topics: Humans; Human Umbilical Vein Endothelial Cells; Friction; Dimethylpolysiloxanes
PubMed: 38842440
DOI: 10.1098/rsif.2023.0696 -
Chemosphere Aug 2024Organotin compounds (OTs) are well studied in various environmental compartments, with a critical focus on the water column as their primary entry point into aquatic...
Organotin compounds (OTs) are well studied in various environmental compartments, with a critical focus on the water column as their primary entry point into aquatic ecosystems. In this context, a method for the analysis of organotin (OTs) in water using silicone rubber-based passive sampling was optimized, validated, and field-tested. Validation covered crucial parameters, including the limit of detection (LOD), limit of quantification (LOQ), accuracy, precision, linearity, and matrix effect. The method was shown to be robust (R ≥ 0.99), with recoveries between 70.2 and 114.6%, and precise (CV < 12.8%) (N = 3). LOD and LOQ were ≤15 and ≤ 48 pg Sn L, respectively, for TBT and TPhT. The matrix effect showed to be low (>-20% ME < 20%) for all OTs but TPhT (69.4%). The silicone rubber-water partition coefficients (Log K) were estimated at 3.37 for MBT, 3.77 for DBT, 4.17 for TBT, 3.49 for MPhT, 3.83 for DPhT, and 4.22 for TPhT. During the field study carried out between October 2021 and February 2022 at the entrance of the Port of Santos navigation channel (Southeastern Brazil), sampling rates ranged between 4.1 and 4.6 L d, and the equilibrium was achieved for MBT, DBT, MPhT, and DPhT after ∼45 days of deployment. The freely dissolved concentrations varied between 134 and 165 pg Sn L for TBT, 388 and 610 pg Sn L for DBT, and 1114 and 1509 pg Sn L for MBT, while MPhT, DPhT, and TPhT were below the limit of detection. Results pointed out that J-FLEX® rubber-based passive sampling is a suitable and reliable alternative method for the continuous monitoring of OTs in the water column.
Topics: Organotin Compounds; Water Pollutants, Chemical; Environmental Monitoring; Silicones; Limit of Detection; Water
PubMed: 38823424
DOI: 10.1016/j.chemosphere.2024.142494 -
Chemical Science May 2024An efficient protocol for the asymmetric synthesis of fluorenols has been developed through an enantioconvergent process enabled by Pd(ii)/chiral norbornene cooperative...
An efficient protocol for the asymmetric synthesis of fluorenols has been developed through an enantioconvergent process enabled by Pd(ii)/chiral norbornene cooperative catalysis. This approach allows facile access to diverse functionalized chiral fluorenols with constantly excellent enantioselectivities, applying readily available racemic secondary -bromobenzyl alcohols and aryl iodides as the starting materials.
PubMed: 38817591
DOI: 10.1039/d4sc01004c -
Neonatal Network : NN May 2024In a sixty-eight-bed level-IV NICU, an increased incidence of hospital-acquired pressure injuries (HAPIs) from noninvasive ventilation (NIV) devices was identified. The...
In a sixty-eight-bed level-IV NICU, an increased incidence of hospital-acquired pressure injuries (HAPIs) from noninvasive ventilation (NIV) devices was identified. The aim of this quality improvement project was to decrease HAPIs from NIV by 10%. A literature review and the Plan-Do-Study-Act were implemented. The intervention included a customized silicone foam dressing under NIV, an NIV skincare bundle, and multidisciplinary support. Hospital-acquired pressure injury rates were tracked over 3 years postinterventions. The incidence of HAPIs declined by 20% from 0.2 per 1,000 patient days to 0.05 per 1,000 patient days. Relative risk was 4.6 times greater prior to intervention ( = .04). Continuous positive airway pressure (CPAP) failure was not noted and measured by the percentage of patients on ventilators pre- and postintervention. Customized silicone foam dressings under NIV, NIV skincare bundle, and multidisciplinary team support may decrease HAPIs in neonates without CPAP failure.
Topics: Humans; Infant, Newborn; Pressure Ulcer; Noninvasive Ventilation; Bandages; Female; Quality Improvement; Intensive Care Units, Neonatal; Male; Silicones; Patient Care Bundles; Skin Care; Iatrogenic Disease
PubMed: 38816221
DOI: 10.1891/NN-2023-0070 -
Biomaterials Advances Sep 2024Conductive elastomers present desirable qualities for sensing pressure in-vivo, such as high piezoresistance in tiny volumes, conformability and, biocompatibility. Many...
Conductive elastomers present desirable qualities for sensing pressure in-vivo, such as high piezoresistance in tiny volumes, conformability and, biocompatibility. Many electrically conductive nanocomposites however, are susceptible to electrical drift following repeated stress cycles and chemical aging. Here we propose an innovative approach to stabilize nanocomposite percolation network against incomplete recovery to improve reproducibility and facilitate sensor calibration. We decouple the tunnelling-percolation network of highly-oriented pyrolytic graphite (HOPG) nanoparticles from the incomplete viscoelastic recovery of the polydimethylsiloxane (PDMS) matrix by inserting minute amounts of insulating SiO nanospheres. SiO nanospheres effectively reduce the number of nearest neighbours at each percolation node switching off the parallel electrical pathways that might become activated under incomplete viscoelastic relaxation. We varied the size of SiO nanospheres and their filling fraction to demonstrate nearly complete piezoresistance recovery when SiO and HOPG nanoparticles have equal diameters (≈400 nm) and SiO and HOPG volume fractions are 1 % and 29.5 % respectively. We demonstrate an in-vivo blood pressure sensor based on this bi-filler composite.
Topics: Nanocomposites; Silicon Dioxide; Graphite; Blood Pressure; Humans; Dimethylpolysiloxanes; Blood Pressure Determination; Electric Conductivity
PubMed: 38815550
DOI: 10.1016/j.bioadv.2024.213905 -
BMC Surgery May 2024To investigate whether simethicone expediates the remission of abdominal distension after laparoscopic cholecystectomy (LC).
OBJECTIVE
To investigate whether simethicone expediates the remission of abdominal distension after laparoscopic cholecystectomy (LC).
METHODS
This retrospective study involved LC patients who either received perioperative simethicone treatment or not. Propensity score matching (PSM) was employed to minimize bias. The primary endpoint was the remission rate of abdominal distension within 24 h after LC. Univariable and multivariable logistic regression analyses were conducted to identify independent risk factors affecting the early remission of abdominal distension after LC. Subsequently, a prediction model was established and validated.
RESULTS
A total of 1,286 patients were divided into simethicone (n = 811) and non-simethicone groups (n = 475) as 2:1 PSM. The patients receiving simethicone had better remission rates of abdominal distension at both 24 h and 48 h after LC (49.2% vs. 34.7%, 83.9% vs. 74.8%, respectively), along with shorter time to the first flatus (14.6 ± 11.1 h vs. 17.2 ± 9.1 h, P < 0.001) compared to those without. Multiple logistic regression identified gallstone (OR = 0.33, P = 0.001), cholecystic polyp (OR = 0.53, P = 0.050), preoperative abdominal distention (OR = 0.63, P = 0.002) and simethicone use (OR = 1.89, P < 0.001) as independent factors contributing to the early remission of abdominal distension following LC. The prognosis model developed for predicting remission rates of abdominal distension within 24 h after LC yielded an area under the curve of 0.643 and internal validation a value of 0.644.
CONCLUSIONS
Simethicone administration significantly enhanced the early remission of post-LC abdominal distension, particularly for patients who had gallstones, cholecystic polyp, prolonged anesthesia or preoperative abdominal distention.
TRIAL REGISTRATION
ChiCTR2200064964 (24/10/2022).
Topics: Humans; Retrospective Studies; Female; Male; Propensity Score; Middle Aged; Cholecystectomy, Laparoscopic; Simethicone; Postoperative Complications; Adult; Treatment Outcome; Aged; Abdomen
PubMed: 38811935
DOI: 10.1186/s12893-024-02460-w