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Chemico-biological Interactions Sep 2023The aerial crop dusting and spraying of fields with the phosphorothioate insecticide parathion in the late 1900s, significantly improved crop yields but resulted in high...
The aerial crop dusting and spraying of fields with the phosphorothioate insecticide parathion in the late 1900s, significantly improved crop yields but resulted in high levels of occupational toxicity in handlers and agricultural workers, as well as cases of intentional self-harm poisoning, culminating in its banning in many western countries by early 2000s. However because of the low solubility and volatility of parathion, most available products were formulated using organic solvents e.g. xylene, to increase the efficacy of the aerosols and dusts. In the present study, the toxicity of parathion was assessed when formulated in an aqueous solvents (ethanol/PBS (1:9)), and delivered to macaques as an aerosol. Doses of 780 μg/kg and 1.56 mg/kg were delivered one day apart, using a modified nebulizer calculated to result in lung deposition of ∼480 μg/kg with a similar or larger amount being swallowed; these doses being similar to the estimated lethal oral dose 286ug/kg - 1.43 mg/kg of formulated parathion in humans. Surprisingly, this dose (a combined amount of ∼14 mg) caused only low AChE inhibition and moderate BChE inhibition with no clinical symptoms, indicating that the use of organic solvents may have previously played a critical role in the severity of parathion toxicity following inhalation exposure. In addition, unlike constitutively toxic OPs, which are highly toxic when inhaled, these results are consistent with the idea that phosphorothioate insecticides appear to be more intoxicating following oral than inhalation exposure. However, this still remains uncertain because the presence of organic solvents in the ingested parathion studies was not always known.
Topics: Humans; Insecticides; Parathion; Solvents; Dose-Response Relationship, Drug; Ethanol; Cholinesterase Inhibitors
PubMed: 37453609
DOI: 10.1016/j.cbi.2023.110635 -
Journal of Environmental Sciences... Nov 2023Fenthion and parathion are two representative kinds of organophosphorus pesticides and widely used in agriculture. They are directly or indirectly released into the...
Fenthion and parathion are two representative kinds of organophosphorus pesticides and widely used in agriculture. They are directly or indirectly released into the atmosphere by spraying or volatilization processes. However, their heterogeneous reactivity toward OH radicals has not yet been well understood. Therefore, this work investigated the heterogeneous kinetics of the OH-initiated degradation of surface-bound fenthion and parathion using a flow reactor. The results showed that OH radicals played an important role in the atmospheric degradation of fenthion and parathion. Their average rate constants were (7.20 ± 0.77) × 10 and (10.40 ± 0.60) × 10 cm/(mol· sec) at a relative humidity (RH) and temperature of 35% and 20 °C, respectively, suggesting that they have relatively short lifetimes in the atmosphere. In addition, a negative RH dependence and a positive temperature dependence of the rate constants were observed. The Arrhenius expressions of fenthion and parathion were k = (1.34 ± 0.48) × 10exp[-(1432.59 ± 105.29)/T] and k = (1.96 ± 1.38) × 10exp[-(1619.98 ± 222.02)/T], respectively, and their overall activation energy was estimated to be (11.88 ± 0.87) and (13.48 ± 1.83) kJ/mol. The experimental results will update the kinetic data of fenthion and parathion in the atmosphere and be helpful to further understand their atmospheric transportation processes.
Topics: Parathion; Fenthion; Pesticides; Organophosphorus Compounds; Kinetics; Hydroxyl Radical
PubMed: 37451785
DOI: 10.1016/j.jes.2022.05.040 -
Cancer Causes & Control : CCC Nov 2023Some pesticides may increase the risk of certain lymphoid malignancies, but few studies have examined Hodgkin lymphoma (HL). In this exploratory study, we examined... (Meta-Analysis)
Meta-Analysis
PURPOSE
Some pesticides may increase the risk of certain lymphoid malignancies, but few studies have examined Hodgkin lymphoma (HL). In this exploratory study, we examined associations between agricultural use of 22 individual active ingredients and 13 chemical groups and HL incidence.
METHODS
We used data from three agricultural cohorts participating in the AGRICOH consortium: the French Agriculture and Cancer Cohort (2005-2009), Cancer in the Norwegian Agricultural Population (1993-2011), and the US Agricultural Health Study (1993-2011). Lifetime pesticide use was estimated from crop-exposure matrices or self-report. Cohort-specific covariate-adjusted overall and age-specific (< 40 or ≥ 40 years) hazard ratios (HRs) and 95% confidence intervals (CIs) were estimated using Cox regression and combined using random effects meta-analysis.
RESULTS
Among 316 270 farmers (75% male) accumulating 3 574 815 person-years at risk, 91 incident cases of HL occurred. We did not observe statistically significant associations for any of the active ingredients or chemical groups studied. The highest risks of HL overall were observed for the pyrethroids deltamethrin (meta-HR = 1.86, 95% CI 0.76-4.52) and esfenvalerate (1.86, 0.78-4.43), and inverse associations of similar magnitude were observed for parathion and glyphosate. Risk of HL at ≥ 40 years of age was highest for ever-use of dicamba (2.04, 0.93-4.50) and lowest for glyphosate (0.46, 0.20-1.07).
CONCLUSION
We report the largest prospective investigation of these associations. Nonetheless, low statistical power, a mixture of histological subtypes and a lack of information on tumour EBV status complicate the interpretability of the results. Most HL cases occurred at older ages, thus we could not explore associations with adolescent or young adult HL. Furthermore, estimates may be attenuated due to non-differential exposure misclassification. Future work should aim to extend follow-up and refine both exposure and outcome classification.
Topics: Young Adult; Adolescent; Humans; Male; Adult; Female; Pesticides; Hodgkin Disease; Prospective Studies; Occupational Exposure; Agriculture
PubMed: 37418114
DOI: 10.1007/s10552-023-01748-1 -
Journal of Hazardous Materials Sep 2023Pesticide misuse has well-documented detrimental effects on ecosystems, with Nile tilapia (Oreochromis niloticus) being particularly vulnerable. The current study...
Pesticide misuse has well-documented detrimental effects on ecosystems, with Nile tilapia (Oreochromis niloticus) being particularly vulnerable. The current study focuses on the impact of widely used sugarcane crop pesticides, Imazapic (IMZ) and Methyl Parathion (MP), on tilapia gill tissues and their lipid membranes. This investigation was motivated by the specific role of the lipid membrane in transport regulation. Bioinspired cell membrane models, including Langmuir monolayers and liposomes (LUVs and GUVs), were utilized to explore the interaction of IMZ and MP. The results revealed electrostatic interactions between IMZ and MP and the polar head groups of lipids, inducing morphological alterations in the lipid bilayer. Tilapia gill tissue exposed to the pesticides exhibited hypertrophic increases in primary and secondary lamellae, total lamellar fusion, vasodilation, and lifting of the secondary lamellar epithelium. These alterations can lead to compromised oxygen absorption by fish and subsequent mortality. This study not only highlights the harmful effects of the pesticides IMZ and MP, but also emphasizes the crucial role of water quality in ecosystem well-being, even at minimal pesticide concentrations. Understanding these impacts can better inform management practices to safeguard aquatic organisms and preserve ecosystem health in pesticide-affected environments.
Topics: Animals; Tilapia; Pesticides; Methyl Parathion; Ecosystem; Cichlids; Lipids; Gills; Water Pollutants, Chemical
PubMed: 37390683
DOI: 10.1016/j.jhazmat.2023.131943 -
Environmental Pollution (Barking, Essex... Oct 2023Parathion and diazinon are two significant organophosphorus pesticides broadly used in agriculture. However, these compounds are toxic and can enter into the environment...
Sulfur-functionalized porphyrin-based covalent organic framework as a metal-free dual-functional catalyst for photodegradation of organophosphorus pesticides under visible-LED-light.
Parathion and diazinon are two significant organophosphorus pesticides broadly used in agriculture. However, these compounds are toxic and can enter into the environment and atmosphere via various processes. Herein, we synthesized and post-functionalized a porphyrinic covalent organic framework (COF), COF-366, with elemental sulfur under solvent-free conditions to give polysulfide-functionalized COF-366, namely PS@COF. The resulting material consisting of porphyrin sensitizer and sulfur nucleophilic sites was used as a dual-functional heterogeneous catalyst for the degradation of these organic compounds using visible-LED-light. Accordingly, the effects of several pertinent parameters such as pH (3-9), the catalyst dosage (5-30 mg), time (up to 80 min), and substrate concentration (10-50 mg L) were studied in detail and optimized. The post-modified COF showed excellent photocatalytic activity (>97%) in the detoxification of diazinon and parathion for 60 min at pH 5.5. Kinetic studies indicated a fast degradation rate with pseudo-second order model for 20 mg L of diazinon and parathion. The total organic carbon detection and gas chromatography-mass spectrometry (GC-MS) confirmed the organic intermediates and byproducts formed during the process. PS@COF displayed good recyclability and high reusable efficiency for six cycles without a noteworthy lose in its catalytic activity, owing to its robust structure.
Topics: Pesticides; Diazinon; Organophosphorus Compounds; Metal-Organic Frameworks; Photolysis; Kinetics; Parathion; Metals; Sulfur
PubMed: 37379874
DOI: 10.1016/j.envpol.2023.122109 -
Talanta Dec 2023To determine parathion in cereals, hydrophilic and hydrophobic deep eutectic solvents (DESs) were used by digital image colorimetry with smartphones. In the solid-liquid...
To determine parathion in cereals, hydrophilic and hydrophobic deep eutectic solvents (DESs) were used by digital image colorimetry with smartphones. In the solid-liquid extraction part, hydrophilic DESs were used as extractants to extract parathion from cereals. In the liquid-liquid microextraction part, hydrophobic DESs dissociated into terpineol and tetrabutylammonium bromide in situ. The dissociated hydrophilic tetrabutylammonium ions reacted with parathion extracted in hydrophilic DESs under alkaline conditions to produce a yellow product, which was extracted and concentrated by dispersed organic phase terpinol. Digital image colorimetry integrated with the use of a smartphone was used for quantitative analysis. The limit of detection and quantification were 0.003 mg kg and 0.01 mg kg, respectively. The recoveries for parathion were 94.8-106.2% with a relative standard deviation less than 3.6%. The proposed method was applied to analyze parathion in cereal samples: the method has the potential to be applied to pesticide residue analysis in food products.
Topics: Solvents; Edible Grain; Smartphone; Deep Eutectic Solvents; Parathion; Colorimetry; Liquid Phase Microextraction; Limit of Detection
PubMed: 37339538
DOI: 10.1016/j.talanta.2023.124831 -
Journal of Hazardous Materials Sep 2023An important public concern worldwide is soil pollution caused by organophosphorus pesticides and their primary metabolites. To protect the public's health, screening...
An important public concern worldwide is soil pollution caused by organophosphorus pesticides and their primary metabolites. To protect the public's health, screening these pollutants on-site and determining their soil bioavailability is important, but doing so is still challenging. This work improved the already-existing organophosphorus pesticide hydrolase (mpd) and transcriptional activator (pobR), and it first designed and constructed a novel biosensor (Escherichia coli BL21/pNP-LacZ) that can precisely detect methyl parathion (MP) and its primary metabolite p-nitrophenol with low background value. To create a paper strip biosensor, E. coli BL21/pNP-LacZ was fixed to filter paper using bio-gel alginate and sensitizer polymyxin B. According to the calibrations of the paper strip biosensor for soil extracts and standard curve, the color intensity of the paper strip biosensor collected by the mobile app may be used to compute the concentration of MP and p-nitrophenol. This method's detection limits were 5.41 µg/kg for p-nitrophenol and 9.57 µg/kg for MP. The detection of p-nitrophenol and MP in laboratory and field soil samples confirmed this procedure. Paper strip biosensor on-site allows for the semi-quantitative measurement of p-nitrophenol and MP levels in soils in a simple, inexpensive, and portable method.
Topics: Methyl Parathion; Pesticides; Organophosphorus Compounds; Soil; Escherichia coli; Biological Availability; Aryldialkylphosphatase; Biosensing Techniques
PubMed: 37295330
DOI: 10.1016/j.jhazmat.2023.131725 -
Neural Networks : the Official Journal... Aug 2023Depression, as a global mental health problem, is lacking effective screening methods that can help with early detection and treatment. This paper aims to facilitate the...
Depression, as a global mental health problem, is lacking effective screening methods that can help with early detection and treatment. This paper aims to facilitate the large-scale screening of depression by focusing on the speech depression detection (SDD) task. Currently, direct modeling on the raw signal yields a large number of parameters, and the existing deep learning-based SDD models mainly use the fixed Mel-scale spectral features as input. However, these features are not designed for depression detection, and the manual settings limit the exploration of fine-grained feature representations. In this paper, we learn the effective representations of the raw signals from an interpretable perspective. Specifically, we present a joint learning framework with attention-guided learnable time-domain filterbanks for depression classification (DALF), which collaborates with the depression filterbanks features learning (DFBL) module and multi-scale spectral attention learning (MSSA) module. DFBL is capable of producing biologically meaningful acoustic features by employing learnable time-domain filters, and MSSA is used to guide the learnable filters to better retain the useful frequency sub-bands. We collect a new dataset, the Neutral Reading-based Audio Corpus (NRAC), to facilitate the research in depression analysis, and we evaluate the performance of DALF on the NRAC and the public DAIC-woz datasets. The experimental results demonstrate that our method outperforms the state-of-the-art SDD methods with an F1 of 78.4% on the DAIC-woz dataset. In particular, DALF achieves F1 scores of 87.3% and 81.7% on two parts of the NRAC dataset. By analyzing the filter coefficients, we find that the most important frequency range identified by our method is 600-700Hz, which corresponds to the Mandarin vowels /e/ and /eˆ/ and can be considered as an effective biomarker for the SDD task. Taken together, our DALF model provides a promising approach to depression detection.
Topics: Depression; Methyl Parathion; Speech; Acoustics
PubMed: 37285730
DOI: 10.1016/j.neunet.2023.05.041 -
Food Chemistry Oct 2023The goal of food safety supervision is to directly identify the pesticide residues on the surface of fruits and vegetables. This study proposed to develop a facile,...
The goal of food safety supervision is to directly identify the pesticide residues on the surface of fruits and vegetables. This study proposed to develop a facile, non-destructive, and sensitive method based on surface-enhanced Raman scattering (SERS) to detect non-systemic pesticides on the surface of fruits and vegetables. The composite material was prepared by loading CTAB guided Au@Ag NRs with positive charge onto filter paper which was modified with PDADMAC(+) and PSS(-) using electrostatic adsorption. Au@Ag NRs with bimetallic synergies were effectively adsorbed in the fiber grid to generate 3D SERS hotspots within a few microns of depth. The results showed that the 3D composite flexible substrate had a high SERS activity, great repeatability, and sensitivity when the method was utilized to detect 4-MBA, methyl-parathion, thiram and chlorpyrifos. Three kinds of non-systemic pesticides on the peel could be detected directly and quickly owing to the arbitrary bending of the substrate, demonstrating the efficiency of the SERS "paste-reading" method. The acquired findings demonstrated that PDADMAC/PSS/Au@Ag NRs composite filter paper had the potential to provide rapid feedback for in-situ analysis of pesticide residues on the surface of fruit and vegetable.
Topics: Pesticides; Fruit; Pesticide Residues; Vegetables; Metal Nanoparticles; Gold; Spectrum Analysis, Raman
PubMed: 37207598
DOI: 10.1016/j.foodchem.2023.136232