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Planta Medica Jun 2024Antimicrobial photodynamic therapy (aPDT) is an evolving treatment strategy against human pathogenic microbes such as the species, including the emerging pathogen ....
Antimicrobial photodynamic therapy (aPDT) is an evolving treatment strategy against human pathogenic microbes such as the species, including the emerging pathogen . Using a modified EUCAST protocol, the light-enhanced antifungal activity of the natural compound parietin was explored. The photoactivity was evaluated against three separate strains of five yeasts, and its molecular mode of action was analysed via several techniques, i.e., cellular uptake, reactive electrophilic species (RES), and singlet oxygen yield. Under experimental conditions ( = 428 nm, H = 30 J/cm, PI = 30 min), microbial growth was inhibited by more than 90% at parietin concentrations as low as c = 0.156 mg/L (0.55 µM) for and , c = 0.313 mg/L (1.10 µM) for , c = 0.625 mg/L (2.20 µM) for , and c = 1.250 mg/L (4.40 µM) for . Mode-of-action analysis demonstrated fungicidal activity. Parietin targets the cell membrane and induces cell death via ROS-mediated lipid peroxidation after light irradiation. In summary, parietin exhibits light-enhanced fungicidal activity against all species tested (including ) and , covering three of the four critical threats on the WHO's most recent fungal priority list.
Topics: Antifungal Agents; Cryptococcus neoformans; Microbial Sensitivity Tests; Candida auris; Light; Candida; Reactive Oxygen Species; Photochemotherapy; Anthraquinones; Photosensitizing Agents
PubMed: 38843798
DOI: 10.1055/a-2249-9110 -
ACS Applied Materials & Interfaces Jun 2024Dental caries is a widespread bacterial infectious disease that imposes a significant public health burden globally. The primary culprits in caries development are...
Dental caries is a widespread bacterial infectious disease that imposes a significant public health burden globally. The primary culprits in caries development are cariogenic bacteria, notably (), due to their robust biofilm-forming capabilities. To address this issue, a series of cationic pyridinium-substituted photosensitizers with aggregation-induced emission have been designed. All of these aggregation-induced emission luminogens (AIEgens) exhibit outstanding microbial visualization and photodynamic killing of , thanks to their luminous fluorescence and efficient singlet oxygen generation ability. Notably, one of the membrane-anchored AIEgens (TDTPY) can inactivate planktic and its biofilm without causing significant cytotoxicity. Importantly, application of TDTPY-mediated photodynamic treatment on in vivo rodent models has yielded commendable imaging results and effectively slowed down caries progression with assured biosafety. Unlike traditional single-mode anticaries materials, AIEgens integrate the dual functions of detecting and removing and are expected to build a new caries management diagnosis and treatment platform. To the best of our knowledge, this is also the first report on the use of AIEgens for anticaries studies both in vitro and in vivo.
Topics: Streptococcus mutans; Photosensitizing Agents; Dental Caries; Photochemotherapy; Animals; Biofilms; Mice; Singlet Oxygen; Humans; Anti-Bacterial Agents
PubMed: 38842123
DOI: 10.1021/acsami.4c04585 -
Journal of Nanobiotechnology Jun 2024Adoptive cellular immunotherapy as a promising and alternative cancer therapy platform is critical for future clinical applications. Natural killer (NK) cells have...
Adoptive cellular immunotherapy as a promising and alternative cancer therapy platform is critical for future clinical applications. Natural killer (NK) cells have attracted attention as an important type of innate immune regulatory cells that can rapidly kill multiple adjacent cancer cells. However, these cells are significantly less effective in treating solid tumors than in treating hematological tumors. Herein, we report the synthesis of a FeO-PEG-CD56/Avastin@Ce6 nanoprobe labeled with NK-92 cells that can be used for adoptive cellular immunotherapy, photodynamic therapy and dual-modality imaging-based in vivo fate tracking. The labeled NK-92 cells specifically target the tumor cells, which increases the amount of cancer cell apoptosis in vitro. Furthermore, the in vivo results indicate that the labeled NK-92 cells can be used for tumor magnetic resonance imaging and fluorescence imaging, adoptive cellular immunotherapy, and photodynamic therapy after tail vein injection. These data show that the developed multifunctional nanostructure is a promising platform for efficient innate immunotherapy, photodynamic treatment and noninvasive therapeutic evaluation of breast cancer.
Topics: Breast Neoplasms; Humans; Female; Killer Cells, Natural; Animals; Photochemotherapy; Mice; Polyethylene Glycols; Cell Line, Tumor; CD56 Antigen; Immunotherapy, Adoptive; Apoptosis; Magnetic Resonance Imaging; Mice, Inbred BALB C; Mice, Nude
PubMed: 38840120
DOI: 10.1186/s12951-024-02599-x -
Lasers in Medical Science Jun 2024The aim of this study was to compare two types of light irradiation devices for antimicrobial photodynamic therapy (aPDT). A 660-nm light-emitting diode (LED) and a... (Comparative Study)
Comparative Study
The aim of this study was to compare two types of light irradiation devices for antimicrobial photodynamic therapy (aPDT). A 660-nm light-emitting diode (LED) and a 665-nm laser diode (LD) were used for light irradiation, and 0.1 mg/L TONS 504, a cationic chlorin derivative, was used as the photosensitizer. We evaluated the light attenuation along the vertical and horizontal directions, temperature rise following light irradiation, and aPDT efficacy against Staphylococcus aureus under different conditions: TONS 504 only, light irradiation only, and TONS 504 with either LED (30 J/cm) or LD light irradiation (continuous: 30 J/cm; pulsed: 20 J/cm at 2/3 duty cycle, 10 J/cm at 1/3 duty cycle). Both LED and LD light intensities were inversely proportional to the square of the vertical distance from the irradiated area. Along the horizontal distance from the nadir of the light source, the LED light intensity attenuated according to the cosine quadrature law, while the LD light intensity did not attenuate within the measurable range. Following light irradiation, the temperature rise increased as the TONS 504 concentration increased in the order of pulsed LD < continuous LD < LED irradiation. aPDT with light irradiation only or TONS 504 only had no antimicrobial effect, while aPDT with TONS 504 under continuous or pulsed LD light irradiation provided approximately 3 log reduction at 30 J/cm and 20 J/cm and approximately 2 log reduction at 10 J/cm. TONS 504-aPDT under pulsed LD light irradiation provided anti-microbial effect without significant temperature rise.
Topics: Photochemotherapy; Staphylococcus aureus; Photosensitizing Agents; Humans; Lasers, Semiconductor; Porphyrins; Temperature
PubMed: 38839711
DOI: 10.1007/s10103-024-04103-1 -
ACS Applied Materials & Interfaces Jun 2024Currently, photodynamic therapy (PDT) is restricted by the laser penetration depth. Except for PDT at 1064 nm wavelength excitation, the development of other...
Currently, photodynamic therapy (PDT) is restricted by the laser penetration depth. Except for PDT at 1064 nm wavelength excitation, the development of other NIR-II-activated nanomaterials with a higher response depth is still hindered and rarely reported in the literature. To overcome these problems, we fabricated a nanoplatform with heterostructures that generate reactive oxygen species (ROS) and ferrite nanoparticles under a high concentration of zinc doping (ZnFeO NPs), which can achieve oxidative damage of tumor cells under near-infrared (NIR) illumination. The recombination of photoelectrons and holes has been markedly inhibited due to the formation of heterostructures in the interfaces, thus greatly enhancing the capability for ROS and oxygen production by modulating the single-component doping content. The efficiency of PDT was verified by and assays under NIR light. Our results revealed that NIR-II (1208 nm) light irradiation of ZnFeO NPs exerted a remarkable antitumor activity, superior to NIR-I light (808 nm). More importantly, the reported ZnFeO NPs strategy provides an opportunity for the success of comparison with light in the first and second near-infrared regions.
Topics: Photochemotherapy; Humans; Zinc; Infrared Rays; Animals; Mice; Reactive Oxygen Species; Photosensitizing Agents; Ferric Compounds; Antineoplastic Agents; Cell Line, Tumor; Mice, Inbred BALB C
PubMed: 38833169
DOI: 10.1021/acsami.4c05717 -
Journal of Materials Chemistry. B Jun 2024In this study, the heavy-atom-free BODIPY dendrimer TM-BDP was synthesized for near-infrared photodynamic therapy, and was composed of a triphenylamine-BODIPY dimer and...
In this study, the heavy-atom-free BODIPY dendrimer TM-BDP was synthesized for near-infrared photodynamic therapy, and was composed of a triphenylamine-BODIPY dimer and four 1-(2-morpholinoethyl)-1-indole-3-ethenyl groups. The TM-BDP could achieve near-infrared photodynamic therapy through two different photosensitive pathways, which include one-photon excitation at 660 nm and two-photon excitation at 1000 nm. In the one-photon excitation pathway, the TM-BDP could generate singlet oxygen and superoxide radicals under 660 nm illumination. In addition, the one-photon PDT experiment in human nasopharyngeal carcinoma (CNE-2) cells also indicated that the TM-BDP could specifically accumulate in lysosomes and show great cell phototoxicity with an IC of 22.1 μM. In the two-photon excitation pathway, the two-photon absorption cross-section at 1030 nm of TM-BDP was determined to be 383 GM, which means that it could generate reactive oxygen species (ROS) under 1000 nm femtosecond laser excitation. Moreover, the two-photon PDT experiment in zebrafish also indicated the TM-BDP could be used for two-photon fluorescence imaging and two-photon induced ROS generation in biological environments. Furthermore, in terms of the ROS generation mechanism, the TM-BDP employed a novel spin-vibronic coupling intersystem crossing (SV-ISC) process for the mechanism of ROS generation and the femtosecond transient absorption spectra indicated that this novel SV-ISC mechanism was closely related to its charge transfer state lifetime. These above experiments of TM-BDP demonstrate that the dendrimer design is an effective strategy for constructing heavy-atom-free BODIPY photosensitizers in the near-infrared region and lay the foundation for two-photon photodynamic therapy in future clinical trials.
Topics: Zebrafish; Animals; Photochemotherapy; Boron Compounds; Dendrimers; Photons; Photosensitizing Agents; Humans; Molecular Structure; Reactive Oxygen Species; Cell Survival; Cell Line, Tumor
PubMed: 38831689
DOI: 10.1039/d4tb00535j -
ACS Macro Letters Jun 2024The low therapeutic efficacy and potential long-term toxicity of antitumor treatments seriously limit the clinical application of phototherapies. Herein, we develop a...
The low therapeutic efficacy and potential long-term toxicity of antitumor treatments seriously limit the clinical application of phototherapies. Herein, we develop a degradable phototheranostic nanoplatform for NIR-II fluorescence bioimaging-guided synergistic photothermal (PTT) and photodynamic therapies (PDT) and immune activation to inhibit tumor growth. The phototheranostic nanoplatform (CX@PSS) consists of multidisulfide-containing polyurethane loaded with a photosensitizer CX, which can be specifically degraded in the GSH overexpressed tumor microenvironment (TME) and exhibits good NIR-II fluorescence, photodynamic, and photothermal properties. Under 808 nm light irradiation, CX@PSS exhibits efficient photothermal conversion and ROS generation, which further induces immunogenic cell death (ICD), releasing tumor-associated antigens and activating the immune response. and studies confirm the potential of CX@PSS in NIR II FL imaging-guided tumor treatments by synergistic PTT, PDT, and immune activation. This work is expected to provide a new pathway for clinical applications of imaging-guided tumor diagnosis and treatments.
Topics: Tumor Microenvironment; Animals; Mice; Humans; Photosensitizing Agents; Theranostic Nanomedicine; Photochemotherapy; Optical Imaging; Infrared Rays; Nanoparticles; Cell Line, Tumor; Neoplasms; Photothermal Therapy; Polyurethanes
PubMed: 38829688
DOI: 10.1021/acsmacrolett.4c00251 -
Journal of Materials Chemistry. B Jun 2024Due to the rapid progression and aggressive metastasis of breast cancer, its diagnosis and treatment remain a great challenge. The simultaneous inhibition of tumor...
Due to the rapid progression and aggressive metastasis of breast cancer, its diagnosis and treatment remain a great challenge. The simultaneous inhibition of tumor growth and metastasis is necessary for breast cancer to obtain ideal therapeutic outcomes. We herein report the development of radioactive hybrid semiconducting polymer nanoparticles (SPN) for imaging-guided tri-modal therapy of breast cancer. Two semiconducting polymers are used to form SPN with a diameter of around 60 nm nano-coprecipitation and they are also labeled with iodine-131 (I) to enhance the imaging functions. The formed SPN show good radiolabeling stability and excellent photodynamic and photothermal effects under 808 nm laser irradiation to produce singlet oxygen (O) and heat. Moreover, SPN can generate O with ultrasound irradiation their sonodynamic properties. After intravenous tail vein injection, SPN can effectively accumulate in the subcutaneous 4T1 tumors of living mice as verified fluorescence and single photon emission computed tomography (SPECT) imaging. With the irradiation of tumors using an 808 nm laser and US, SPN mediate photodynamic therapy (PDT), photothermal therapy (PTT) and sonodynamic therapy (SDT) to kill tumor cells. Such a tri-modal therapy leads to an improved efficacy in inhibiting tumor growth and suppressing tumor metastasis compared to the sole SDT and combinational PDT-PTT. This study thus demonstrates the applications of SPN to diagnose tumors and combine different therapies for effective breast cancer treatment.
Topics: Animals; Nanoparticles; Mice; Female; Polymers; Iodine Radioisotopes; Breast Neoplasms; Semiconductors; Photochemotherapy; Mice, Inbred BALB C; Humans; Cell Proliferation; Antineoplastic Agents; Cell Line, Tumor; Particle Size; Tomography, Emission-Computed, Single-Photon; Photothermal Therapy; Mammary Neoplasms, Experimental
PubMed: 38828732
DOI: 10.1039/d4tb00834k -
Spectrochimica Acta. Part A, Molecular... Oct 2024Considering the increasing number of pathogens resistant to commonly used antibiotics as well as antiseptics, there is an urgent need for antimicrobial approaches that...
Considering the increasing number of pathogens resistant to commonly used antibiotics as well as antiseptics, there is an urgent need for antimicrobial approaches that can effectively inactivate pathogens without the risk of establishing resistance. An alternative approach in this context is antibacterial photodynamic therapy (APDT). APDT is a process that involves bacterial cell death using appropriate wavelength light energy and photosensitizer and causes the production of reactive oxygen species inside or outside the microbial cell depending on the penetration of light energy. In our study, a new porphyrin compound 4,4'-methylenebis(2-((E)-((4-(10,15,20-triphenylporphyrin-5-yl)phenyl)imino)methyl)phenol) (SP) was designed and synthesized as photosensitizer and its structure was clarified by NMR (C and H) and mass determination method. Photophysical and photochemical properties were examined in detail using different methods. Singlet oxygen quantum yields were obtained as 0.48 and 0.59 by direct and indirect methods, respectively. Antibacterial activity studies have been conducted within the scope of biological activity and promising results have been obtained under LED light (500-700 nm, 265 V, 1500 LM), contributing to the antibacterial photodynamic therapy literature.
Topics: Photochemotherapy; Photosensitizing Agents; Porphyrins; Anti-Bacterial Agents; Singlet Oxygen; Microbial Sensitivity Tests; Light; Bacteria; Drug Design
PubMed: 38824758
DOI: 10.1016/j.saa.2024.124529 -
Journal of Colloid and Interface Science Oct 2024Chemotherapy and surgery stand as primary cancer treatments, yet the unique traits of the tumor microenvironment hinder their effectiveness. The natural compound...
Chemotherapy and surgery stand as primary cancer treatments, yet the unique traits of the tumor microenvironment hinder their effectiveness. The natural compound epigallocatechin gallate (EGCG) possesses potent anti-tumor and antibacterial traits. However, the tumor's adaptability to chemotherapy due to its acidic pH and elevated glutathione (GSH) levels, coupled with the challenges posed by drug-resistant bacterial infections post-surgery, impede treatment outcomes. To address these challenges, researchers strive to explore innovative treatment strategies, such as multimodal combination therapy. This study successfully synthesized Cu-EGCG, a metal-polyphenol network, and detailly characterized it by using synchrotron radiation and high-resolution mass spectrometry (HRMS). Through chemodynamic therapy (CDT), photothermal therapy (PTT), and photodynamic therapy (PDT), Cu-EGCG showed robust antitumor and antibacterial effects. Cu in Cu-EGCG actively participates in a Fenton-like reaction, generating hydroxyl radicals (·OH) upon exposure to hydrogen peroxide (HO) and converting to Cu. This Cu interacts with GSH, weakening the oxidative stress response of bacteria and tumor cells. Density functional theory (DFT) calculations verified Cu-EGCG's efficient GSH consumption during its reaction with GSH. Additionally, Cu-EGCG exhibited outstanding photothermal conversion when exposed to 808 nm near-infrared (NIR) radiation and produced singlet oxygen (O) upon laser irradiation. In both mouse tumor and wound models, Cu-EGCG showcased remarkable antitumor and antibacterial properties.
Topics: Anti-Bacterial Agents; Copper; Nanocomposites; Antineoplastic Agents; Animals; Mice; Humans; Catechin; Microbial Sensitivity Tests; Drug Resistance, Bacterial; Photochemotherapy; Wound Infection; Drug Screening Assays, Antitumor; Staphylococcus aureus; Photothermal Therapy; Particle Size; Escherichia coli; Cell Survival; Cell Line, Tumor; Surface Properties; Cell Proliferation
PubMed: 38824748
DOI: 10.1016/j.jcis.2024.05.080