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Plants (Basel, Switzerland) Dec 2023The legacy effects of invasive plant species can hinder the recovery of native communities, especially under nitrogen deposition conditions, where invasive species show...
The legacy effects of invasive plant species can hinder the recovery of native communities, especially under nitrogen deposition conditions, where invasive species show growth advantages and trigger secondary invasions in controlled areas. Therefore, it is crucial to thoroughly investigate the effects of nitrogen deposition on the legacy effects of plant invasions and their mechanisms. The hypotheses of this study are as follows: (1) Nitrogen deposition amplifies the legacy effects of plant invasion. This phenomenon was investigated by analysing four potential mechanisms covering community system structure, nitrogen metabolism, geochemical cycles, and microbial mechanisms. The results suggest that microorganisms drive plant-soil feedback processes, even regulating or limiting other factors. (2) The impact of nitrogen deposition on the legacy effects of plant invasions may be intensified primarily through enhanced nitrogen metabolism via microbial anaerobes bacteria. Essential insights into invasion ecology and ecological management have been provided by analysing how nitrogen-fixing bacteria improve nitrogen metabolism and establish sustainable methods for controlling invasive plant species. This in-depth study contributes to our better understanding of the lasting effects of plant invasions on ecosystems and provides valuable guidance for future ecological management.
PubMed: 38202380
DOI: 10.3390/plants13010072 -
Computational Biology and Chemistry Apr 2024Extensive research has accumulated which suggests that phosphatidylinositol 3-kinase delta (PI3Kδ) is closely related to the occurrence and development of various human...
Extensive research has accumulated which suggests that phosphatidylinositol 3-kinase delta (PI3Kδ) is closely related to the occurrence and development of various human diseases, making PI3Kδ a highly promising drug target. However, PI3Kδ exhibits high homology with other members of the PI3K family, which poses significant challenges to the development of PI3Kδ inhibitors. Therefore, in the present study, a hybrid virtual screening (VS) approach based on a ligand-based pharmacophore model and multicomplex-based molecular docking was developed to find novel PI3Kδ inhibitors. 13 crystal structures of the human PI3Kδ-inhibitor complex were collected to establish models. The inhibitors were extracted from the crystal structures to generate the common feature pharmacophore. The crystallographic protein structures were used to construct a naïve Bayesian classification model that integrates molecular docking based on multiple PI3Kδ conformations. Subsequently, three VS protocols involving sequential or parallel molecular docking and pharmacophore approaches were employed. External predictions demonstrated that the protocol combining molecular docking and pharmacophore resulted in a significant improvement in the enrichment of active PI3Kδ inhibitors. Finally, the optimal VS method was utilized for virtual screening against a large chemical database, and some potential hit compounds were identified. We hope that the developed VS strategy will provide valuable guidance for the discovery of novel PI3Kδ inhibitors.
Topics: Humans; Molecular Docking Simulation; Phosphatidylinositol 3-Kinases; Phosphatidylinositol 3-Kinase; Protein Kinase Inhibitors; Pharmacophore; Bayes Theorem; Ligands
PubMed: 38198965
DOI: 10.1016/j.compbiolchem.2023.108011 -
American Journal of Cancer Research 2023This study aimed to summarize the current developments and hub genes in the ferroptosis field using bibliometrics and bioinformatics and provide guidance for future...
This study aimed to summarize the current developments and hub genes in the ferroptosis field using bibliometrics and bioinformatics and provide guidance for future developments. The publications on ferroptosis from 2012 to 2021 were extracted from the Web of Science database. VOSviewer software and CiteSpace software were used to visualize and predict the trend of ferroptosis research. The key genes related to ferroptosis were selected from the Web of Genecards, and Kyoto Encyclopedia of Genes and Genomes (KEGG)/Gene Ontology (GO) analysis was performed. Cytoscape software and online survival curve analysis platform were also used to screen hub genes and analyze their roles. Chinese researchers published the highest number of publications in this field, while American publications exhibited higher quality. In terms of institutions, Central South University and Zhejiang University have the highest number of publications. published more studies than other journals. The application of ferroptosis is a major research area, and, importantly, "RCD", "FTH1", and "nomogram" are the keywords. We also found tumor-related pathways of interest in the field of ferroptosis. genes were of significance for the prognosis of tumors. The number of publications on ferroptosis may increase in the future. Cooperation among countries and disciplines is particularly important in this regard. Also, the applications of ferroptosis, especially in chemotherapy and immunotherapy for tumors, will be the focus of future research. Keywords "RCD", "FTH1", and "nomogram" is receiving high attention, and in-depth studies on tumor-related genes , , and may provide new therapeutic targets.
PubMed: 38187041
DOI: No ID Found -
BMC Plant Biology Jan 2024As a vital type of noncoding RNAs, circular RNAs (circRNAs) play important roles in plant growth and development and stress response. However, little is known about the...
BACKGROUND
As a vital type of noncoding RNAs, circular RNAs (circRNAs) play important roles in plant growth and development and stress response. However, little is known about the biological roles of circRNAs in regulating the stability of male fertility restoration for cytoplasmic male sterility (CMS) conditioned by Gossypium harknessii cytoplasm (CMS-D2) cotton under high-temperature (HT) stress.
RESULTS
In this study, RNA-sequencing and bioinformatics analysis were performed on pollen grains of isonuclear alloplasmic near-isogenic restorer lines NH [N(Rfrf)] and SH [S(Rfrf)] with obvious differences in fertility stability under HT stress at two environments. A total of 967 circRNAs were identified, with 250 differentially expressed under HT stress. We confirmed the back-splicing sites of eight selected circRNAs using divergent primers and Sanger sequencing. Tissue-specific expression patterns of five differentially expressed circRNAs (DECs) were also verified by RT-PCR and qRT-PCR. Functional enrichment and metabolic pathway analysis revealed that the parental genes of DECs were significantly enriched in fertility-related biological processes such as pollen tube guidance and cell wall organization, as well as the Pentose and glucuronate interconversions, Steroid biosynthesis, and N-Glycan biosynthesis pathways. Moreover, we also constructed a putative circRNA-mediated competing endogenous RNA (ceRNA) network consisting of 21 DECs, eight predicted circRNA-binding miRNAs, and their corresponding 22 mRNA targets, especially the two ceRNA modules circRNA346-miR159a-MYB33 and circRNA484-miR319e-MYB33, which might play important biological roles in regulating pollen fertility stability of cotton CMS-D2 restorer line under HT stress.
CONCLUSIONS
Through systematic analysis of the abundance, characteristics and expression patterns of circRNAs, as well as the potential functions of their parent genes, our findings suggested that circRNAs and their mediated ceRNA networks acted vital biological roles in cotton pollen development, and might be also essential regulators for fertility stability of CMS-D2 restorer line under heat stress. This study will open a new door for further unlocking complex regulatory mechanisms underpinning the fertility restoration stability for CMS-D2 in cotton.
Topics: Gossypium; RNA, Circular; Cytoplasm; Fertility; RNA; Heat-Shock Response
PubMed: 38183049
DOI: 10.1186/s12870-023-04706-w -
Chemistry (Weinheim An Der Bergstrasse,... Mar 2024Li-S batteries possess high energy density and have been one of the most promising energy storage systems. For sulfur cathodes, the electrochemical performance is still...
Li-S batteries possess high energy density and have been one of the most promising energy storage systems. For sulfur cathodes, the electrochemical performance is still seriously hindered by the polysulfide shuttling and sluggish conversion kinetics. It has been demonstrated to be one effective strategy to address the above issues via designing electrocatalysts with robust affinity and catalytic capacity towards polysulfides. However, it is still a great challenge to rapidly and economically discover high-performance electrocatalysts. Herein, using density functional theory calculation, we studied the chemisorption-catalysis behavior of sulfur species on a series of electrocatalysts (MCo X , M=Co, Zn, Cu, Ni, Fe, and Mn, X=O, S, and Se) to assess the effect of the anions and cations co-regulation on their electronic structure, chemisorption behavior, and catalytic property. FeCo Se and CuCo Se combined appropriate chemisorption with superior electronic conductivity and sulfur reduction catalytic capacity have been predicted as novel electrocatalysts for high-performance Li-S batteries. This study gives theoretical guidance for rapid discovery of high-efficient electrocatalyst to boost the electrochemical performance of sulfur cathodes.
PubMed: 38164045
DOI: 10.1002/chem.202303285 -
Frontiers in Psychiatry 2023Depression affects the development of adolescents and makes it difficult for them to adapt to future life. The purpose of this study was to elucidate the population...
BACKGROUND
Depression affects the development of adolescents and makes it difficult for them to adapt to future life. The purpose of this study was to elucidate the population characteristics of adolescent depression.
METHODS
This study measured depression based on the Patient Health Questionnaire-9 items and sociodemographic questionnaire. A total of 8,235 valid questionnaires were collected from six schools in Haikou and Qionghai, Hainan Province, covering the ages of 13 to 18. The questionnaires included high schools with multiple levels, including general high schools, key high schools, and vocational high schools. Latent category analysis (LCA) was used to identify potential categories of depressive symptoms among adolescents. Latent Class Analysis (LCA) was used for determining depressive symptom latent categories and their proportional distribution among adolescents.
RESULTS
LCA analysis divided the data into 3 categories, namely no depression, low depression, and high depression groups. The percentage of the high depression group was 10.1%, and that of the low depression group was 48.4%. The Jorden index was greatest for a PHQ-9 score of 14.5. The 1 grade of junior middle school students entered the high and low depression groups 1.72 and 1.33 times more often than seniors. The number of the 1 grade of high school students included in the high and low depression groups was 1.55 and 1.42 times of the 3 grade of high school students group. The detection rate of the high depression group of vocational school adolescents was 13.5%, which was significantly higher than that of key high schools (9.6%) and general high schools (9.0%).
CONCLUSION
This study found that 1 grade of junior middle school students and the 1 grade of high school students were more likely to fall into depressive conditions. Moreover, Adolescent girls require more attention than boys. Vocational school students need more psychological guidance.
PubMed: 38152357
DOI: 10.3389/fpsyt.2023.1182024 -
Orphanet Journal of Rare Diseases Dec 2023Despite the increasing incidence of aplastic anemia in China, few studies have explored its effect on the patients' quality of life from the perspective of these...
BACKGROUND
Despite the increasing incidence of aplastic anemia in China, few studies have explored its effect on the patients' quality of life from the perspective of these patients. In fact, patients with aplastic disorder live with the disease for a long time, and need to face a variety of difficult realities, including multiple disease symptoms and drug side effects, heavy burden of medical costs, difficulties in social reintegration, and negative emotional distress. Therefore, this study used descriptive qualitative research to explore the direct and rich quality-of-life experiences of patients with aplastic anemia.
METHODS
A total of 19 patients with aplastic anemia were recruited in this study using purposive sampling combined with maximum variation strategy. 5 of the patients with AA were from northern China, and the others were from southern China. Data were collected using semi-structured interviews and analyzed using the conventional content analysis method.
RESULTS
This study yielded important information about the experiences of patients with aplastic anemia in China. The content analysis method finally identified 3 themes and 9 sub-themes, including: physical symptoms (declining physical capacity, treatment-related symptoms, changes in body image), psychological symptoms (mood changes related to the stage of the disease, change in self-image, growth resulting from the disease experience), social burden (decline in career development, perceived burden to the family, social stigma). Patients with AA from different regions didn't show much difference in quality of life.
CONCLUSIONS
Aplastic anemia affects the physical, psychological, and social aspects of patients' lives. Therefore, health care providers need to consider the patients' physical response and psychological feelings to provide relevant medical guidance and multi-channel social support that would improve their confidence and quality of life.
CLINICAL TRIAL REGISTRATION
Name: Development and preliminary application of Quality of Life Scale for Patients with Aplastic Anemia. Number: ChiCTR2100047575. URL: http://www.chictr.org.cn/login.aspx?referurl=%2flistbycreater.aspx .
Topics: Humans; Anemia, Aplastic; Quality of Life; Qualitative Research; Emotions; China
PubMed: 38129869
DOI: 10.1186/s13023-023-02993-y -
Zhonghua Shao Shang Yu Chuang Mian Xiu... Dec 2023To develop a risk prediction model for the recurrence of diabetic foot ulcer (DFU) in diabetic patients and primarily validate its predictive value. Meta-analysis... (Meta-Analysis)
Meta-Analysis
To develop a risk prediction model for the recurrence of diabetic foot ulcer (DFU) in diabetic patients and primarily validate its predictive value. Meta-analysis combined with retrospective cohort study was conducted. The Chinese and English papers on risk factors related to DFU recurrence publicly published in China Biology Medicine disc, China National Knowledge Infrastructure, Wanfang Database, VIP Database, and PubMed, Embase, Cochrane Library, and Web of Science, and the search time was from the establishment date of each database until March 31, 2022. The papers were screened and evaluated, the data were extracted, a meta-analysis was performed using RevMan 5.4.1 statistical software to screen risk factors for DFU recurrence, and Egger's linear regression was used to assess the publication bias of the study results. Risk factors for DFU recurrence mentioned in ≥3 studies and with statistically significant differences in the meta-analysis were selected as the independent variables to develop a logistic regression model for risk prediction of DFU recurrence. The medical records of 101 patients with DFU who met the inclusion criteria and were admitted to Affiliated Hospital of Guizhou Medical University from January 2019 to June 2022 were collected. There were 69 males and 32 females, aged (63±14) years. The receiver operating characteristic (ROC) curve of the predictive performance of the above constructed predictive model for DFU recurrence was drawn, and the area under the ROC curve, maximum Youden index, and sensitivity and specificity at the point were calculated. Dataset including data of 8 risk factors for DFU recurrence and the DFU recurrence rates of 10 000 cases was simulated using RStudio software and a scatter plot was drawn to determine two probabilities for risk division of DFU recurrence. Using the coefficients corresponding to 8 DFU recurrence risk factors ×10 and taking the integer as the score of coefficient weight of each risk factor, the total score was obtained by summing up, and the cutoff scores for risk level division were calculated based on the total score × two probabilities for risk division of DFU recurrence. Finally, 20 papers were included, including 3 case-control studies and 17 cohort studies, with a total of 4 238 cases and DFU recurrence rate of 22.7% to 71.2%. Meta-analysis showed that glycosylated hemoglobin >7.5% and with plantar ulcer, diabetic peripheral neuropathy, diabetic peripheral vascular disease, smoking, osteomyelitis, history of amputation/toe amputation, and multidrug-resistant bacterial infection were risk factors for the recurrence of DFU (with odds ratios of 3.27, 3.66, 4.05, 3.94, 1.98, 7.17, 11.96, 3.61, 95% confidence intervals of 2.79-3.84, 2.06-6.50, 2.50-6.58, 2.65-5.84, 1.65-2.38, 2.29-22.47, 4.60-31.14, 3.13-4.17, respectively, <0.05). There were no statistically significant differences in publication biases of diabetic peripheral neuropathy, diabetic peripheral vascular disease, glycosylated hemoglobin >7.5%, plantar ulcer, smoking, multidrug-resistant bacterial infection, or osteomyelitis (>0.05), but there was a statistically significant difference in the publication bias of amputation/toe amputation (=-30.39, <0.05). The area under the ROC curve of the predictive model was 0.81 (with 95% confidence interval of 0.71-0.91) and the maximum Youden index was 0.59, at which the sensitivity was 72% and the specificity was 86%. Ultimately, 29.0% and 44.8% were identified respectively as the cutoff for dividing the probability of low risk and medium risk, and medium risk and high risk for DFU recurrence, while the corresponding total scores of low, medium, and high risks of DFU recurrence were <37, 37-57, and 58-118, respectively. Eight risk factors for DFU recurrence are screened through meta-analysis and the risk prediction model for DFU recurrence is developed, which has moderate predictive accuracy and can provide guidance for healthcare workers to take interventions for patient with DFU recurrence risk.
Topics: Male; Female; Humans; Diabetic Foot; Diabetic Neuropathies; Foot Ulcer; Retrospective Studies; Glycated Hemoglobin; Risk Factors; Osteomyelitis; Peripheral Vascular Diseases; Bacterial Infections; Diabetes Mellitus
PubMed: 38129301
DOI: 10.3760/cma.j.cn501225-20231101-00166 -
Journal of Clinical Nursing Jul 2024While previous research has established that resilience is affected by various factors, these studies have primarily focussed on individual variables associated with... (Observational Study)
Observational Study
BACKGROUND
While previous research has established that resilience is affected by various factors, these studies have primarily focussed on individual variables associated with resilience, without providing insights into how to influence the rate of change in resilience.
AIM
To examine the trajectory of resilience and identify the factors associated with changes in resilience among spousal caregivers of patients with newly diagnosed advanced cancer receiving treatment during the first 6 months.
DESIGN
An observational longitudinal study.
METHODS
A total of 312 spousal caregivers of patients with newly diagnosed advanced cancer were recruited from January 2022 to December 2022 in Yancheng, China. Three data collection points were established, spanning from the first month to 6 months after initial cancer treatment. A latent growth model was employed to depict the resilience trajectory at various time points. A latent growth model with time-invariant covariates was adopted to determine the factors influencing resilience trajectory. The study adhered to the STROBE checklist for proper reporting.
RESULTS
Throughout the follow-up period, the participants experienced a significant increase in resilience. Gender, family income, the patient's health status, spirituality and belief in familism were significantly associated with the baseline resilience level. Moreover, family income, the patient's health status, spirituality, caregiver burden and belief in familism were significantly associated with the rate of resilience change over time.
CONCLUSIONS
Spousal caregivers demonstrated a linear increase in resilience during the first 6 months after initial treatment. Meanwhile, changes in resilience were influenced by multiple factors during the early phase of cancer treatment. Thus, more attention should be paid to early identification and implementation of targeted interventions.
RELEVANCE TO CLINICAL PRACTICE
Healthcare professionals should understand the change in resilience among spousal caregivers and conduct timely mental health interventions to enhance the resilience of families affected by cancer.
PATIENT OR PUBLIC CONTRIBUTION
The Guidance for Reporting Involvement of Patients and the Public-Short Form reporting checklists were used to improve patient and public involvement.
Topics: Humans; Neoplasms; Male; Female; Resilience, Psychological; Caregivers; Middle Aged; Longitudinal Studies; China; Spouses; Adult; Aged; Adaptation, Psychological
PubMed: 38108199
DOI: 10.1111/jocn.16972 -
Journal of Child and Adolescent... Feb 2024This research aimed to examine changes caused by the COVID-19 pandemic in activities of daily living and health status of children with special needs.
OBJECTIVE
This research aimed to examine changes caused by the COVID-19 pandemic in activities of daily living and health status of children with special needs.
METHOD
The current descriptive and comparative study was carried out with 82 students studying at a special education practice school that provided daytime education to children with special needs before and during the COVID-19 pandemic. The Personal Information Form, the Katz Index of Independence in Activities of Daily Living, and the Omaha System Problem Classification Scheme were employed as data collection tools in the research.
RESULTS
The 10 most common problems during the COVID-19 pandemic were stated as cognition (86.6%), mental health (75.6%), speech and language (85.4%), interpersonal relationships (79.3%), personal care (79.3%), social contact (64.6%), growth and development (63.4%), neighborhood/workplace safety (58.5%), communication with community resources (57.3%), and caretaking/parenting (n = 47, 57.3%). The incidence of "cognition," "speech and language," "neighborhood/workplace safety," "communication with community resources," and "caretaking/parenting" problems of children with special needs was significantly higher during the pandemic than before the pandemic (p < 0.05; p < 0.001).
CONCLUSION
The current research emphasized the significant impacts on the health of children with physical and mental disabilities and loss of opportunity, as their education was considerably interrupted during the quarantine. During a pandemic, the regular evaluation of health benefits/risks is essential to ensure the continuity of medical follow-up, rehabilitation, education, and support mechanisms for children with disabilities.
Topics: Child; Humans; COVID-19; Activities of Daily Living; Disabled Children; Pandemics; Schools
PubMed: 38102988
DOI: 10.1111/jcap.12446