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Heliyon Jun 2024Among new technologies aimed at improving water productivity, nanotechnology have been identified as effective means of enhancing the properties of agricultural water....
Among new technologies aimed at improving water productivity, nanotechnology have been identified as effective means of enhancing the properties of agricultural water. Building on the synergy of plasma and nanobubbles, this study explored the combination of plasma electric discharge and nanocavitation as a novel approach for soilless cultivation. The study aimed to investigate the impact of this hybrid technology on hydroponics nutrient solution. Additionally, the study further aimed to assess the effect of both technologies individually, as well as various application periods, including 3, 9, and 15 min for hybrid technology use. The study employed the nutrient flow technique to hydroponically cultivate lettuce. The findings showed that the application of each technology individually did not significantly increase yield. However, the hybrid technology treatment for 9 min resulted in a significant yield increase of almost 60 %. This improvement can be attributed to the stability, solubility, and absorption of products resulting from plasma treatment, as well as the antimicrobial and anti-algae effects of both technologies. Additionally, an increase in flavonoid and potassium content and a decrease in iron were observed in plants grown under optimal treatment. Overall, this study demonstrated the potential for synergy between plasma and nanobubble techniques in hydroponic culture.
PubMed: 38912454
DOI: 10.1016/j.heliyon.2024.e32578 -
Innovation (Cambridge (Mass.)) Jul 2024Stony corals, the primary architects of coral reef ecosystems, are largely underrepresented in omics studies despite their importance. The presence of endosymbiotic... (Review)
Review
Stony corals, the primary architects of coral reef ecosystems, are largely underrepresented in omics studies despite their importance. The presence of endosymbiotic Symbiodiniaceae algae complicates the extraction of pure coral DNA, posing a challenge for genomic research. Here, we devised a comprehensive methodological framework that incorporates various experimental treatments to achieve 99% purity in coral DNA extraction and a robust bioinformatics pipeline to guarantee the assembly of high-quality, contamination-free coral genomes. Validation of our framework using samples demonstrated its efficacy and superiority in obtaining high-quality pure coral genomes using easily accessible adult colony. This integrated framework serves as a critical foundation for large-scale genome-enabled research on stony corals, providing insight into coral evolution and conservation.
PubMed: 38912429
DOI: 10.1016/j.xinn.2024.100643 -
Nature Water 2024Understanding the impacts of microplastics (MPs) on aqueous environments requires understanding their transport dynamics and how their presence affects other natural...
Understanding the impacts of microplastics (MPs) on aqueous environments requires understanding their transport dynamics and how their presence affects other natural processes and cycles. In this context, one aspect to consider is how MPs interact with freshwater snow (FWS), a mixture of algae and natural particles. FWS is one of the primary drivers of the flux of organic matter from the water surface to the bottom sediment, where zooplankton, diurnal migration, fish faecal pellets settling and turbulent mixing can also play prominent roles. Understanding how MPs and FWS heteroaggregation affects their respective settling velocities is important to assess not only MPs fate and transport but also their ecological impacts by altering FWS deposition and thereby nutrient cycling. In this present study, we obtained a mechanistic understanding of the processes controlling MPs settling dynamics and heteroaggregation with FWS and the subsequent impacts on the settling rates of both MPs and ballasted FWS. Here we used a plexiglass column equipped with a stereoscopic camera system to track the settling velocities of (1) MPs of various compositions, densities and morphologies, (2) FWS flocs and (3) MP-FWS agglomerates. For each experimental set, thousands of particles were tracked over a series of image sequences. We found that agglomerates with high-density MPs settled at least twofold faster than FWS alone, implying a much smaller residence time in the water column, except for cases with MP fibres or low-density plastics. These findings will help to refine MP fate models and, while contingent on MPs number, may impact biogeochemical cycles by changing the flux of nutrients contained in FWS to the sediment.
PubMed: 38912368
DOI: 10.1038/s44221-024-00248-z -
Frontiers in Public Health 2024Microplastics (MPs) are particles with a diameter of <5 mm. The disposal of plastic waste into the environment poses a significant and pressing issue concern globally.... (Review)
Review
Microplastics (MPs) are particles with a diameter of <5 mm. The disposal of plastic waste into the environment poses a significant and pressing issue concern globally. Growing worry has been expressed in recent years over the impact of MPs on both human health and the entire natural ecosystem. MPs impact the feeding and digestive capabilities of marine organisms, as well as hinder the development of plant roots and leaves. Numerous studies have shown that the majority of individuals consume substantial quantities of MPs either through their dietary intake or by inhaling them. MPs have been identified in various human biological samples, such as lungs, stool, placenta, sputum, breast milk, liver, and blood. MPs can cause various illnesses in humans, depending on how they enter the body. Healthy and sustainable ecosystems depend on the proper functioning of microbiota, however, MPs disrupt the balance of microbiota. Also, due to their high surface area compared to their volume and chemical characteristics, MPs act as pollutant absorbers in different environments. Multiple policies and initiatives exist at both the domestic and global levels to mitigate pollution caused by MPs. Various techniques are currently employed to remove MPs, such as biodegradation, filtration systems, incineration, landfill disposal, and recycling, among others. In this review, we will discuss the sources and types of MPs, the presence of MPs in different environments and food, the impact of MPs on human health and microbiota, mechanisms of pollutant adsorption on MPs, and the methods of removing MPs with algae and microbes.
Topics: Humans; Microplastics; Ecosystem; Water Pollutants, Chemical; Environmental Monitoring
PubMed: 38912266
DOI: 10.3389/fpubh.2024.1411389 -
Open Life Sciences 2024Recent advancements in protein/enzyme engineering have enabled the production of a diverse array of high-value compounds in microbial systems with the potential for... (Review)
Review
Recent advancements in protein/enzyme engineering have enabled the production of a diverse array of high-value compounds in microbial systems with the potential for industrial applications. The goal of this review is to articulate some of the most recent protein engineering advances in bacteria, yeast, and other microbial systems to produce valuable substances. These high-value substances include α-farnesene, vitamin B12, fumaric acid, linalool, glucaric acid, carminic acid, mycosporine-like amino acids, patchoulol, orcinol glucoside, d-lactic acid, keratinase, α-glucanotransferases, β-glucosidase, seleno-methylselenocysteine, fatty acids, high-efficiency β-glucosidase enzymes, cellulase, β-carotene, physcion, and glucoamylase. Additionally, recent advances in enzyme engineering for enhancing thermostability will be discussed. These findings have the potential to revolutionize various industries, including biotechnology, food, pharmaceuticals, and biofuels.
PubMed: 38911927
DOI: 10.1515/biol-2022-0856 -
Plant Communications Jun 2024
PubMed: 38909281
DOI: 10.1016/j.xplc.2024.101007 -
Scientific Reports Jun 2024This study investigated the first-ever reported use of freshwater Nannochloropsis for the bioremediation of dairy processing side streams and co-generation of valuable...
This study investigated the first-ever reported use of freshwater Nannochloropsis for the bioremediation of dairy processing side streams and co-generation of valuable products, such as β-galactosidase enzyme. In this study, N. limnetica was found to grow rapidly on both autoclaved and non-autoclaved whey-powder media (referred to dairy processing by-product or DPBP) without the need of salinity adjustment or nutrient additions, achieving a biomass concentration of 1.05-1.36 g L after 8 days. The species secreted extracellular β-galactosidase (up to 40.84 ± 0.23 U L) in order to hydrolyse lactose in DPBP media into monosaccharides prior to absorption into biomass, demonstrating a mixotrophic pathway for lactose assimilation. The species was highly effective as a bioremediation agent, being able to remove > 80% of total nitrogen and phosphate in the DPBP medium within two days across all cultures. Population analysis using flow cytometry and multi-channel/multi-staining methods revealed that the culture grown on non-autoclaved medium contained a high initial bacterial load, comprising both contaminating bacteria in the medium and phycosphere bacteria associated with the microalgae. In both autoclaved and non-autoclaved DPBP media, Nannochloropsis cells were able to establish a stable microalgae-bacteria interaction, suppressing bacterial takeover and emerging as dominant population (53-80% of total cells) in the cultures. The extent of microalgal dominance, however, was less prominent in the non-autoclaved media. High initial bacterial loads in these cultures had mixed effects on microalgal performance, promoting β-galactosidase synthesis on the one hand while competing for nutrients and retarding microalgal growth on the other. These results alluded to the need of effective pre-treatment step to manage bacterial population in microalgal cultures on DPBP. Overall, N. limnetica cultures displayed competitive β-galactosidase productivity and propensity for efficient nutrient removal on DPBP medium, demonstrating their promising nature for use in the valorisation of dairy side streams.
Topics: beta-Galactosidase; Microalgae; Whey; Lactose; Stramenopiles; Fresh Water; Biodegradation, Environmental; Biomass; Nitrogen
PubMed: 38906947
DOI: 10.1038/s41598-024-65146-6 -
Environmental Research Jun 2024Urbanization of estuaries drastically changed existing shorelines and bathymetric contours, in turn modifying habitat for marine foundational species that host critical...
Urbanization of estuaries drastically changed existing shorelines and bathymetric contours, in turn modifying habitat for marine foundational species that host critical biodiversity. And yet we lack approaches to characterize a significant fraction of the biota that inhabit these ecosystems on time scales that align with rates of urbanization. Environmental DNA (or eDNA) metabarcoding that combines multiple assays targeting a broad range of taxonomic groups can provide a solution, but we need to determine whether the biological communities it detects ally with different habitats in these changing aquatic environments. In this study, we tested whether tree of life metabarcoding (ToL-metabarcoding) data extracted from filtered seawater samples correlated with four known geomorphic habitat zones across a heavily urbanized estuary (Sydney Harbour, Australia). Using this method, we substantially expanded our knowledge on the composition and spatial distribution of marine biodiversity across the tree of life in Sydney Harbour, particularly for organisms where existing records are sparse. Excluding terrestrial DNA inputs, we identified significant effects of both distance from the mouth of Sydney Harbour and geomorphic zone on biological community structure in the ToL-metabarcoding dataset (entire community), as well as in each of the taxonomic subgroups that we considered (fish, macroinvertebrates, algae and aquatic plants, bacteria). This effect appeared to be driven by taxa as a collective versus a few individual taxa, with each taxon explaining no more than 0.62% of the variation between geomorphic zones. Similarly, taxonomic richness was significantly higher within geomorphic zones with large sample sizes, but also decreased by 1% with each additional kilometer from the estuary mouth, a result consistent with a reduction in tidal inputs and available habitat in upper catchments. Based on these results, we suggest that ToL-metabarcoding can be used to benchmark biological monitoring in other urbanized estuaries globally, and in Sydney Harbour at future time points based on detection of bioindicators across the tree of life. We also suggest that robust biotic snapshots can be archived following extensive curation of taxonomic assignments that incorporates ecological affinities, supported by records from relevant and regional biodiversity repositories.
PubMed: 38906450
DOI: 10.1016/j.envres.2024.119454 -
Ecotoxicology and Environmental Safety Jun 2024Copper (Cu) is a necessary mineral nutrient for plant growth and development and is involved in several morphological, physiological, and biochemical processes; however,...
Integrated physiological, biochemical, and transcriptomic analyses of Bruguiera gymnorhiza leaves under long-term copper stress: Stomatal size, wax crystals and composition.
Copper (Cu) is a necessary mineral nutrient for plant growth and development and is involved in several morphological, physiological, and biochemical processes; however, high concentrations of Cu can negatively impact these processes. The role of stomata in responding to various biotic and abiotic stimuli has not been studied in Bruguiera gymnorhiza, particularly in terms of their coordinated interactions at the molecular, physiological, and biochemical levels. Moreover, numerous plants employ strategies such as the presence of thick waxy cuticles on their leaf epidermis and the closing of stomata to reduce water loss. Thus, this study investigates the accumulation of Cu in B. gymnorhiza and its effect on leaf morphology and the molecular response under different Cu treatments (0, 200, and 400 mg L⁻¹, Cu0, Cu200, and Cu400, respectively) during a two years stress period. The results show that Cu stress affected accumulation and transport, increased the activities of peroxidase and ascorbate peroxidase, concentrations of soluble sugar, proline, and HO, and decreased the activity of catalase and content of malondialdehyde. Also, Cu-induced stress decreased the uptake of phosphorus and nitrogen and inhibited plant photosynthesis, which consequently led to reduced plant growth. Scanning electron microscopy combined with gas chromatography-mass spectrometry showed that B. gymnorhiza leaves had higher wax crystals and compositions under increased Cu stress, which forced the leaf's stomata to be closed. Also, the contents of alkanes, alcohols, primary alcohol levels (C26:0, C28:0, C30:0, and C32:0), n-Alkanes (C29 and C30), and other wax loads were significantly higher, while fatty acid (C12, C16, and C18) was lower in Cu200 and Cu400 compared to Cu0. Furthermore, the transcriptomic analyses revealed 1240 (771 up- and 469 downregulated), 1000 (723 up- and 277 down-regulated), and 1476 (808 up- and 668 downregulated) differentially expressed genes in Cu0 vs Cu200, Cu0 vs Cu400, and Cu200 vs Cu400, respectively. RNA-seq analyses showed that Cu mainly affected eight pathways, including photosynthesis, cutin, suberin, and wax biosynthesis. This study provides a reference for understanding mangrove response to heavy metal stress and developing novel management practices.
PubMed: 38905937
DOI: 10.1016/j.ecoenv.2024.116609 -
Journal of Environmental Management Jun 2024In many industrial processes a large amount of water with high salinity is co-produced whose treatment poses considerable challenges to the available technologies. The...
In many industrial processes a large amount of water with high salinity is co-produced whose treatment poses considerable challenges to the available technologies. The produced water (PW) from offshore operations is currently being discharged to sea without treatment for dissolved pollutants due to space limitations. A biofilter on the seabed adjacent to a production platform would negate all size restrictions, thus reducing the environmental impact of oil and gas production offshore. The moving bed biofilm reactor (MBBR) was investigated for PW treatment from different oilfields in the North Sea at 10 °C and 40 °C, corresponding to the sea and PW temperature, respectively. The six PW samples in study were characterized by high salinity and chemical oxygen demand with ecotoxic effects on marine algae S. pseudocostatum (0.4%
PubMed: 38905793
DOI: 10.1016/j.jenvman.2024.121391