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Journal of Pharmacy & Bioallied Sciences Apr 2024The aim of the in vitro study was to compare the internal merge and marginal perfection of polyetheretherketone (PEEK) and polyetherketoneketone (PEKK) crowns under a...
PURPOSE
The aim of the in vitro study was to compare the internal merge and marginal perfection of polyetheretherketone (PEEK) and polyetherketoneketone (PEKK) crowns under a stereomicroscope.
METHODS
All-ceramic preparation is done on the maxillary first premolar which is mounted on the wax block which was scanned using 3Shape scanner and duplicated into 30 acrylic CAD/CAM dies ( = 15) for the placement of PEEK and PEKK crowns. The YENADENT milling system was used to fabricate 15 samples from Group A PEEK crowns and to fabricate 15 samples from Group B PEKK crowns. According to the manufacturer's instructions, an equal amount of the dual-cure resin luting agent (Rely X U200 Self-Adhesive resin, 3M, Germany) was dispensed on the mixing pad before being mixed with cement and painted on the internal surfaces of the copings with the finger pressure crowns which were luted. The values were then analyzed using one-way ANOVA (post hoc) followed by Dunnett's -test.
RESULTS
Among those two groups, PEEK group materials showed the lowest mean value of (28.73.3 ± 4.01) for marginal fit and (26.72 ± 2.53) for internal gap, whereas PEKK group showed a mean value of (32.85 ± 4.63) and (33.06 ± 4.14), respectively.
CONCLUSION
Among these two groups, comparatively less marginal fit and internal adaptation is seen in PEKK when compared to the PEEK crowns. While the marginal fit and internal adaptation of both PEEK and PEKK copings were in acceptable clinical range.
PubMed: 38882827
DOI: 10.4103/jpbs.jpbs_756_23 -
Journal of Pharmacy & Bioallied Sciences Apr 2024Bioactivity refers to the ability of a material to interact with living organisms or biological systems in a way that elicits a specific response. In the context of...
BACKGROUND
Bioactivity refers to the ability of a material to interact with living organisms or biological systems in a way that elicits a specific response. In the context of materials science and medicine, bioactivity is particularly important because it can determine the suitability of material for various applications.
OBJECTIVE
To evaluate and compare different commercially available calcium silicate-based materials regarding: 1. Morphological and elemental analysis at the dentin/material interface. 2. Calcium and silicon release and uptake by adjacent root canal dentine by evaluating the calcium and silicon incorporation depth in adjacent root canal dentin.
MATERIALS AND METHODS
This study examined four calcium silicate-based cements: Biodentine, MTA Angelus, BioAggregate, and MTA Plus. One hundred extracted human teeth with intact apices and no cavities were selected. Root sections measuring 3 mm in length were created at the mid-root level using low-speed diamond discs. Bioactivity was evaluated at 1, 7, 30, and 90 days, respectively.
RESULTS
The principal composition of the interfacial dentine layer and incorporation of calcium and silicon into dentine was measured at 1, 7, 30, and 90 days. Statistical analysis was performed by multiple comparisons using Tukey HSD.
CONCLUSION
All the materials have shown bioactivity, i.e. release of calcium, silicon, and their uptake in the adjacent dentin in the presence of phosphate-buffered saline.
PubMed: 38882788
DOI: 10.4103/jpbs.jpbs_959_23 -
Journal of Pharmacy & Bioallied Sciences Apr 2024To evaluate the fracture resistance (FR) of the teeth having reenacted perforating internal resorption cavities repaired by distinctive calcium silicate-based cements...
Reinforcement of Teeth Having Reenacted Perforating Internal Resorption Cavities After Repair Using Different Calcium Silicate-based Cements and Backfilling Materials: An Study.
To evaluate the fracture resistance (FR) of the teeth having reenacted perforating internal resorption cavities repaired by distinctive calcium silicate-based cements (CSCs) specifically: Endocem MTA, Biodentine, NeoMTA Plus, and backfilling materials. Ninety-six freshly extracted human mandibular premolar teeth were selected. Twelve roots were used as the negative control group. Rotary files were used to complete the final irrigation and root canal preparation on the remaining teeth. Following that, burs were used to make standardized internal resorption chambers in the middle part of the roots. Twelve of these samples were used as positive control samples. The remaining 72 root canals were obturated in the apical 4 mm using a single-cone approach, and they were separated into 6 groups based on the CSCs used to fill voids and the materials used as backfilling. Group 1: Endocem MTA (resorption) + Endocem MTA (coronal), Group 2: Endocem MTA (resorption) + Gutta-percha/sealer (coronal), Group 3: Biodentine (resorption) + Biodentine (coronal), Group 4: Biodentine (resorption) + Gutta-percha/sealer (coronal), Group 5: NeoMTA Plus (resorption) + NeoMTA Plus (coronal), and Group 6: NeoMTA Plus (resorption) + Gutta-percha/sealer (coronal). Specimens were inserted in acrylic resin and then subjected to fracture testing. Fracture strength tests were performed using a Universal Testing Machine. The force was employed vertically with a consistent speed of 1 mm/minute. The results were analyzed with Variance and Bonferrini tests at < 0.005. The mean force of fracture values were 447.00, 201.25, 318.75, 187.50, 596.58, 258.75, 347.50, and 298.75 N for Group 1, 2, 3, 4, 5, 6, 7, and 8, respectively. "There was a significant difference ( < 0.001) between the experimental groups and the control group". Group 5 showed the highest FR as compared to other groups. Backfilling with CSCs appears to be a better material than a gutta-percha/sealer combination. Neo MTA plus furthermore appeared the highest fracture-resistant material, while Biodentine + Gutta percha/sealer showed the least FR.
PubMed: 38882772
DOI: 10.4103/jpbs.jpbs_791_23 -
Journal of Pharmacy & Bioallied Sciences Apr 2024To assess clinically and histologically the efficacy of TheraCal LC and Biodentine as Direct Pulp Capping agents.
AIM
To assess clinically and histologically the efficacy of TheraCal LC and Biodentine as Direct Pulp Capping agents.
MATERIALS AND METHOD
Sixty caries-free maxillary first premolar teeth which were scheduled for orthodontic extraction were selected for the study and were divided equally into Group 1 (Theracal LC) and Group 2 (Biodentine). Thermal and electric pulp testing was performed on the teeth to assess pulp vitality followed by tooth preparation to expose pulp horns under local anesthesia. After achieving hemostasis, the direct pulp capping agent was placed and composite restoration was done. All the teeth were evaluated and compared in terms of clinical and histological findings after 6 weeks. The statistical analysis used was the Kruskal-Wallis test.
RESULTS
Teeth with Theracal LC showed no sensitivity to heat, cold, or percussion, whereas sensitivity to heat, cold, or percussion was noted with Biodentine. Histological evaluation revealed that TheraCal LC has higher efficiency in complete dentin bridge formation, had maximum dentin thickness, and showed minimal or no pulpal disorganization and pulpal inflammation as compared to Biodentine.
CONCLUSION
TheraCal LC proves to be a promising future as a direct pulp capping agent.
PubMed: 38882760
DOI: 10.4103/jpbs.jpbs_805_23 -
Journal of Dental Research, Dental... 2024Insufficient information exists regarding the fracture resistance and failure pattern of newly developed zirconia-reinforced lithium disilicate (ZL, Vita Ambria) onlays....
BACKGROUND
Insufficient information exists regarding the fracture resistance and failure pattern of newly developed zirconia-reinforced lithium disilicate (ZL, Vita Ambria) onlays. This in vitro study compared the fracture resistance of two types of onlays: monolithic lithium disilicate (LD) and monolithic ZL.
METHODS
Forty-eight ceramic onlay restorations were fabricated on epoxy dies using a maxillary first premolar model. The samples were divided into two main groups: LD and ZL. Half of each group was subjected to thermomechanical fatigue loading (TML) using a chewing simulator. All the samples were cemented with self-adhesive resin cement. Subsequently, they were loaded until failure in a universal testing machine, and the fracture patterns and resistance were recorded.
RESULTS
Before TML, ZL demonstrated the highest statistically significant mean fracture resistance (499.76±34.14N) compared to LD (470.40±27.38N). After TML, ZL showed the highest non-statistically significant mean fracture resistance (429.27±131.42N), while LD's mean fracture resistance decreased (377.31±62.18N).
CONCLUSION
Monolithic zirconia-reinforced onlays demonstrated higher fracture resistance and a more favorable failure mode compared to LD. However, the impact of thermomechanical aging resulted in reduced fracture resistance for both materials, with a notable preference observed for ZL.
PubMed: 38881646
DOI: 10.34172/joddd.40843 -
Dental Materials : Official Publication... Jun 2024Quality control testing of dental materials requires a standard to enable the generation of reproducible and comparable data. Currently there are no standards for...
OBJECTIVES
Quality control testing of dental materials requires a standard to enable the generation of reproducible and comparable data. Currently there are no standards for testing materials used for vital pulp therapy. The aim of this study was to develop a new standard to evaluate solubility of pulp preservation materials.
METHODS
The solubility of three materials used for vital pulp therapy: Biodentine, TheraCal and Activa was evaluated using two international standards for dental materials ISO 4049:2019 (S1) and ISO 6876:2012 (S2). For both standards, a modified methodology was evaluated. This included changing the volume of the solution used (S1M, S2M), using Dulbecco's modified eagle medium (DMEM) as an alternative to water (S1D, S2D) and periodic solution change for the ISO 4049 method (S1P, S1MP). Materials were characterised before and after completion of solubility test using scanning electron microscopy (SEM) and X-ray diffraction (XRD) analysis.
RESULTS
The test materials exhibited different solubility values depending on the methodology used. Biodentine exhibited significantly lower solubility when lower volumes of solution were used when tested using both ISO methods (p ≤ 0.05). TheraCal and Activa showed negative solubility values after desiccation when tested using ISO 4049:2019. The Biodentine exhibited changes in its microstructure which was dependent on the method used to test solubility.
CONCLUSIONS
The solubility values obtained were dependent on the method used. It is thus important to use methods that replicate the clinical environment for meaningful evaluations.
PubMed: 38876829
DOI: 10.1016/j.dental.2024.05.028 -
Biomedicine & Pharmacotherapy =... Jul 2024Pulp therapy has been emerged as a one of the efficient therapies in the field of endodontics. Among different types of new endodontic materials, pulpotec has been...
Pulp therapy has been emerged as a one of the efficient therapies in the field of endodontics. Among different types of new endodontic materials, pulpotec has been materialized as a recognized material for vital pulp therapy. However, its efficacy has been challenged due to lack of information about its cellular biocompatibility. This study evaluates the mechanistic biocompatibility of pulpotec cement with macrophage cells (RAW 264.7) at cellular and molecular level. The biocompatibility was evaluated using experimental and computational techniques like MTT assay, oxidative stress analysis and apoptosis analysis through flow cytometry and fluorescent microscopy. The results showed concentration-dependent cytotoxicity of pulpotec cement extract to RAW 264.7 cells with an LC 50 of X/10-X/20. The computational analysis depicted the molecular interaction of pulpotec cement extract components with metabolic proteins like Sod1 and p53. The study revealed the effects of Pulpotec cement's extract, showing a concentration-dependent induction of oxidative stress and apoptosis. These effects were due to influential structural and functional abnormalities in the Sod1 and p53 proteins, caused by their molecular interaction with internalized components of Pulpotec cement. The study provided a detailed view on the utility of Pulpotec in endodontic applications, highlighting its biomedical aspects.
Topics: Oxidative Stress; Apoptosis; Mice; Animals; RAW 264.7 Cells; Macrophages; Biocompatible Materials; Materials Testing; Cell Survival; Tumor Suppressor Protein p53; Dental Cements; Superoxide Dismutase-1
PubMed: 38870628
DOI: 10.1016/j.biopha.2024.116921 -
International Dental Journal Jun 2024The aim was to assess the peri-implant clinicoradiographic status and prostaglandin E2 (PGE2) levels in peri-implant sulcular fluid (PISF) samples collected from...
BACKGROUND
The aim was to assess the peri-implant clinicoradiographic status and prostaglandin E2 (PGE2) levels in peri-implant sulcular fluid (PISF) samples collected from individuals with cement-retained and crew-retained implants.
METHODS
In this observational study, participants with cement-retained and screw-retained implants were enrolled. A questionnaire was utilized to gather demographic information and assess the educational background of the participants. Peri-implant modified plaque and bleeding indices, probing depth, and crestal bone loss were measured. Subsequently, PISF samples were collected, and corresponding volumes were recorded. Commercial kits employing enzyme-linked immunosorbent assay were employed to quantify PGE2 levels. The sample size was determined, and group comparisons were conducted using the Student t test and the Mann-Whitney U-test. Logistic regression models were constructed to evaluate the correlation between PGE2 levels and clinicoradiographic and demographics. The predefined level of significance was established at P < .05.
RESULTS
Sixty-seven participants, consisting of 33 with cement-retained implants and 34 with screw-retained implants, were included in the study. The mean ages for individuals with cement and screw-retained implants were 54.2 ± 8.7 and 58.7 ± 7.4 years, respectively. The majority of participants had completed university-level education. Reportedly, 87.9% and 82.4% of individuals with cement and screw-retained implants, respectively brushed teeth twice daily. No significant differences were observed in clinicoradiographic parameters, PGE2 volume, and levels between cement-retained and screw-retained implants. There was no correlation between PGE2 levels and peri-implant clinicoradiographic parameters among individuals with either cement-retained or screw-retained implants.
CONCLUSIONS
Cement-retained and screw-retained implants exhibit a consistent peri-implant clinicoradiographic status, accompanied by stable levels of PGE2 in PISF provided oral hygiene maintenance regimens are stringently followed.
PubMed: 38866672
DOI: 10.1016/j.identj.2024.04.026 -
Cureus May 2024Silver amalgam, glass ionomer, resin-modified glass ionomer, compomers, light polymerized hybrid composite resin, and hybrid glass ionomer are among the most frequent...
INTRODUCTION
Silver amalgam, glass ionomer, resin-modified glass ionomer, compomers, light polymerized hybrid composite resin, and hybrid glass ionomer are among the most frequent restorative materials used as cavity-based or post-endodontics. Thus, to meet the needs of both patients and dentists, Cention N reimagines the traditional filling by integrating bulk placement, ion release, and durability into a dual-curing, aesthetically pleasing solution. Hoewver, we do not have enough information from studies comparing this hybrid restorative material's shear bond strengths to dentin to draw any firm conclusions. Cention N, zirconomer, and Vitremer are three hybrid tooth-colored restorative materials that were evaluated for their shear bond strength to dentin. This research aimed to compare and evaluate these materials.
METHODOLOGY
The purpose of this research was to use a universal Instron machine to measure the shear bond stress of three distinct hybrid tooth-colored restorative materials in relation to dentin. The research samples consisted of 45 extracted lower first premolars from humans. The teeth were then assigned into three groups of 15 samples each according to different color acrylic resin blocks, namely, group A (pink acrylic blocks), which had Cention in cement; group B (white acrylic blocks), which has zirconomer cement; and group C (violet acrylic blocks), which had Vitremer cement.
RESULTS
There was no statistically significant difference between the three groups and the normal distribution, as shown by the negligible values in the tests involving the three groups. Put simply, each of the three categories exhibits data that follows a normal distribution. This allows for further data analysis to be conducted using the parametric test of significance.
CONCLUSION
The shear bond strength of hybrid glass ionomer restorative materials has to be further investigated in both laboratory and living organism settings.
PubMed: 38864074
DOI: 10.7759/cureus.60123 -
Clinical Implant Dentistry and Related... Jun 2024To present the 3-year clinical, radiographic, and aesthetic outcomes in patients with congenitally missing lateral incisors rehabilitated with two narrow-diameter...
Clinical, radiographic, and aesthetic outcomes at two narrow-diameter implants to replace congenital missing maxillary lateral incisors: A 3-year prospective, clinical study.
INTRODUCTION
To present the 3-year clinical, radiographic, and aesthetic outcomes in patients with congenitally missing lateral incisors rehabilitated with two narrow-diameter implants (NDIs).
METHODS
The original population consisted of 100 patients rehabilitated with a cement-retained bi-layered zirconia single-unit crown supported by either a Ø2.9 mm (Test) or a Ø3.3 mm (Control) NDI (n = 50). At the 1- and 3-year follow-up (T2, T3), implant survival rate, crestal bone level (CBL) changes, biological, and technical complications were recorded, while the assessment of the aesthetic outcomes was performed using the Copenhagen Index Score.
RESULTS
Seventy-four patients Ø2.9 mm (n = 39) or Ø3.3 mm (n = 35) reached T3, as 24 patients were lost to follow-up and 1 implant (Ø3.3 mm) was removed. Throughout the observation period, minimal CBL changes (i.e., <1 mm) were detected between groups. Despite the positive aesthetic scores recorded (i.e., 1-2), at T3 20% of patients rehabilitated with a Ø3.3 mm versus 2.6% of patients Ø2.9 mm displayed an alveolar process deficiency (Score 3). No additional technical and/or mechanical complications were recorded between T2 and T3. Tooth vitality was maintained in all neighboring teeth. Peri-implant probing depths and plaque scores remained low in both groups (p > 0.05).
CONCLUSION
The use of 2.9 or 3.3 diameter implants showed comparable favorable mid-term results in terms of survival rate, CBL, and aesthetic outcomes. Hence, clinicians should rely on the use of such NDIs when replacing maxillary lateral incisors.
PubMed: 38863078
DOI: 10.1111/cid.13339