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Cell Reports May 2024Quorum sensing (QS) is a cell-to-cell communication mechanism mediated by small diffusible signaling molecules. Previous studies showed that RpfR controls Burkholderia...
Quorum sensing (QS) is a cell-to-cell communication mechanism mediated by small diffusible signaling molecules. Previous studies showed that RpfR controls Burkholderia cenocepacia virulence as a cis-2-dodecenoic acid (BDSF) QS signal receptor. Here, we report that the fatty acyl-CoA ligase DsfR (BCAM2136), which efficiently catalyzes in vitro synthesis of lauryl-CoA and oleoyl-CoA from lauric acid and oleic acid, respectively, acts as a global transcriptional regulator to control B. cenocepacia virulence by sensing BDSF. We show that BDSF binds to DsfR with high affinity and enhances the binding of DsfR to the promoter DNA regions of target genes. Furthermore, we demonstrate that the homolog of DsfR in B. lata, RS02960, binds to the target gene promoter, and perception of BDSF enhances the binding activity of RS02960. Together, these results provide insights into the evolved unusual functions of DsfR that control bacterial virulence as a response regulator of QS signal.
Topics: Quorum Sensing; Burkholderia cenocepacia; Virulence; Bacterial Proteins; Coenzyme A Ligases; Gene Expression Regulation, Bacterial; Promoter Regions, Genetic; Animals; Signal Transduction; Fatty Acids, Monounsaturated; Mice; Protein Binding; Lauric Acids
PubMed: 38748879
DOI: 10.1016/j.celrep.2024.114223 -
Molecular Plant-microbe Interactions :... Jan 2024Numerous bacterial species employ diffusible signal factor (DSF)-based quorum sensing (QS) as a widely conserved cell-cell signaling communication system to collectively... (Review)
Review
Numerous bacterial species employ diffusible signal factor (DSF)-based quorum sensing (QS) as a widely conserved cell-cell signaling communication system to collectively regulate various behaviors crucial for responding to environmental changes. -11-Methyl-dodecenoic acid, known as DSF, was first identified as a signaling molecule in pv. . Subsequently, many structurally related molecules have been identified in different bacterial species. This review aims to provide an overview of current understanding regarding the biosynthesis and regulatory role of DSF signals in both pathogenic bacteria and a biocontrol bacterium. Recent studies have revealed that the DSF-based QS system regulates antimicrobial factor production in a cyclic dimeric GMP-independent manner in the biocontrol bacterium . Additionally, the DSF family signals have been found to be involved in suppressing plant innate immunity. The discovery of these diverse signaling mechanisms holds significant promise for developing novel strategies to combat stubborn plant pathogens. [Formula: see text] Copyright © 2024 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.
Topics: Quorum Sensing; Xanthomonas campestris; Signal Transduction; Cyclic GMP; Bacterial Proteins
PubMed: 37880815
DOI: 10.1094/MPMI-05-23-0074-CR -
Applied and Environmental Microbiology Oct 2023Outer membrane vesicle (OMV)-delivered quinolone signal (PQS) plays a critical role in cell-cell communication in . However, the functions and mechanisms of...
Outer membrane vesicle (OMV)-delivered quinolone signal (PQS) plays a critical role in cell-cell communication in . However, the functions and mechanisms of membrane-enclosed PQS in interspecies communication in microbial communities are not clear. Here, we demonstrate that PQS delivered by both OMVs from and liposome reduces the competitiveness of , which usually shares the same niche in the lungs of cystic fibrosis patients, by interfering with quorum sensing (QS) in through the LysR-type regulator ShvR. Intriguingly, we found that ShvR regulates the production of the QS signals cis-2-dodecenoic acid (BDSF) and N-acyl homoserine lactone (AHL) by directly binding to the promoters of signal synthase-encoding genes. Perception of PQS influences the regulatory activity of ShvR and thus ultimately reduces QS signal production and virulence in . Our findings provide insights into the interspecies communication mediated by the membrane-enclosed QS signal among bacterial species residing in the same microbial community.IMPORTANCEQuorum sensing (QS) is a ubiquitous cell-to-cell communication mechanism. Previous studies showed that mainly employs cis-2-dodecenoic acid (BDSF) and N-acyl homoserine lactone (AHL) QS systems to regulate biological functions and virulence. Here, we demonstrate that quinolone signal (PQS) delivered by outer membrane vesicles from or liposome attenuates virulence by targeting the LysR-type regulator ShvR, which regulates the production of the QS signals BDSF and AHL in a. Our results not only suggest the important roles of membrane-enclosed PQS in interspecies and interkingdom communications but also provide a new perspective on the use of functional nanocarriers loaded with QS inhibitors for treating pathogen infections.
Topics: Humans; Quorum Sensing; Virulence; Acyl-Butyrolactones; Liposomes; Bacterial Proteins; Burkholderia cenocepacia; Pseudomonas aeruginosa; Gene Expression Regulation, Bacterial
PubMed: 37796010
DOI: 10.1128/aem.01184-23