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Microbiology Resource Announcements Mar 2024Here, we report the draft genome sequences of strains zfem001-005, five isolates from the intestinal microbiota of healthy larval zebrafish at a developmental age of 7...
Here, we report the draft genome sequences of strains zfem001-005, five isolates from the intestinal microbiota of healthy larval zebrafish at a developmental age of 7 days post fertilization. The isolates have been identified as , , , , and , respectively.
PubMed: 38376340
DOI: 10.1128/mra.00934-23 -
Journal of Global Antimicrobial... Mar 2024The contamination of fresh surface waters poses a significant burden on human health and prosperity, especially in marginalized communities with limited resources and...
OBJECTIVES
The contamination of fresh surface waters poses a significant burden on human health and prosperity, especially in marginalized communities with limited resources and inadequate infrastructure. Here, we performed in-depth genomic analyses of multidrug-resistant bacteria (MDR-B) isolated from Al-Oueik river water that is used for irrigation of agricultural fields in a disenfranchised area that also hosts a makeshift Syrian refugee camp.
METHODS
A composite freshwater sample was filtered. Faecal coliforms were counted and extended spectrum cephalosporins and/or ertapenem resistant bacteria were screened. Isolates were identified using MALDI-TOF-MS and analysed using whole-genome sequencing (WGS) to identify the resistome, sequence types, plasmid types, and virulence genes.
RESULTS
Approximately 10 CFU/100 mL of faecal coliforms were detected in the water. Four drug-resistant Gram-negative bacteria were identified, namely Escherichia coli, Klebsiella pneumoniae, Enterobacter hormaechei, and Pseudomonas otitidis. Notably, the E. coli isolate harboured bla and a YRIN-inserted PBP3, representing an emerging public health challenge. The K. pneumoniae isolate carried bla as well as mutations in the gene encoding the OmpK37 porin. Enterobacter hormaechei and P. otitidis harboured bla and bla, respectively.
CONCLUSION
This report provides comprehensive genomic analyses of MDR-B in irrigation water in Lebanon. Our results further support that irrigation water contaminated with faecal material can be a reservoir of important MDR-B, which can spread to adjacent agricultural fields and other water bodies, posing both public health and food safety issues. Therefore, there is an urgent need to implement effective water quality monitoring and management programs to control the proliferation of antibiotic-resistant pathogens in irrigation water in Lebanon.
Topics: Humans; Escherichia coli; Rivers; Enterobacter; Plasmids; Klebsiella pneumoniae; Gram-Negative Bacteria
PubMed: 38154747
DOI: 10.1016/j.jgar.2023.12.016 -
BMC Infectious Diseases Dec 2023Pseudomonas otitidis belongs to the genus Pseudomonas and causes various infections, including ear, skin, and soft tissue infections. P. otitidis has a unique... (Review)
Review
BACKGROUND
Pseudomonas otitidis belongs to the genus Pseudomonas and causes various infections, including ear, skin, and soft tissue infections. P. otitidis has a unique susceptibility profile, being susceptible to penicillins and cephalosporins but resistant to carbapenems, due to the production of the metallo-β-lactamase called POM-1. This revealed genetic similarities with Pseudomonas aeruginosa, which can sometimes lead to misidentification.
CASE PRESENTATION
We report the case of a 70-year-old Japanese male who developed cellulitis and bacteremia during chemotherapy for multiple myeloma. He was initially treated with meropenem, but blood culture later revealed gram-negative bacilli identified as P. otitidis using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). Carbapenem resistance was predicted from previous reports; therefore, we switched to dual therapy with levofloxacin and cefepime, and favorable treatment results were obtained.
CONCLUSION
This is the first reported case of P. otitidis cellulitis and bacteremia in an immunocompromised patient. Carbapenems are typically used in immunocompromised patients and P. otitidis is often resistant to it. However, its biochemical properties are similar to those of Pseudomonas aeruginosa; therefore, its accurate identification is critical. In the present study, we rapidly identified P. otitidis using MALDI-TOF MS and switched from carbapenems to an appropriate antimicrobial therapy, resulting in a successful outcome.
Topics: Humans; Male; Aged; Anti-Bacterial Agents; Cellulitis; Pseudomonas; Carbapenems; Pseudomonas Infections; Pseudomonas aeruginosa; Bacteremia; Immunocompromised Host; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
PubMed: 38110897
DOI: 10.1186/s12879-023-08919-0