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Oecologia Mar 2024Procambarus clarkii is a notorious invasive species that has led to ecological concerns owing to its high viability and rapid reproduction. South Korea, a country...
Procambarus clarkii is a notorious invasive species that has led to ecological concerns owing to its high viability and rapid reproduction. South Korea, a country exposed to a high risk of introduction of invasive species due to active international trade, has suffered from recent massive invasions by invasive species, necessitating the evaluation of potential areas requiring intensive monitoring. In this study, we developed two different types of species distribution models, CLIMEX and random forest, for P. clarkii using occurrence records from the United States. The potential distribution in the United States was predicted along coastal lines and inland regions located below 40°N latitude The model was then applied to evaluate the potential distribution in South Korea, and an ensemble map was constructed to identify the most vulnerable domestic regions. According to both models, the domestic potential distribution was highest in most areas located at low altitudes. In the ensemble model, most of the low-altitude western regions, the eastern coast, and some southern inland regions were predicted to be suitable for the distribution of P. clarkii, and a similar distribution pattern was predicted when the model was projected into the future climate. Through this study, it is possible to secure basic data that can be used for the early monitoring of the introduction and subsequent distribution of P. clarkii.
Topics: Animals; Astacoidea; Commerce; Climate Change; Internationality; Introduced Species; Moths
PubMed: 38386057
DOI: 10.1007/s00442-024-05516-z -
Fish & Shellfish Immunology Apr 2024Antimicrobial peptides (AMPs), which are widely present in animals and plants, have a broad distribution, strong broad-spectrum antibacterial activity, low likelihood of...
Effects of antimicrobial peptides from dietary Hermetia illucens larvae on the growth, immunity, gene expression, intestinal microbiota and resistance to Aeromonas hydrophila of juvenile red claw crayfish (Cherax quadricarinatus).
Antimicrobial peptides (AMPs), which are widely present in animals and plants, have a broad distribution, strong broad-spectrum antibacterial activity, low likelihood of developing drug resistance, high thermal stability and antiviral properties. The present study investigated the effects of adding AMPs from Hermetia illucens larvae on the growth performance, muscle composition, antioxidant capacity, immune response, gene expression, antibacterial ability and intestinal microbiota of Cherax quadricarinatus (red claw crayfish). Five experimental diets were prepared by adding 50 (M1), 100 (M2), 150 (M3) and 200 (M4) mg/kg of crude AMP extract from H. illucens larvae to the basal diet feed, which was also used as the control (M0). After an eight-week feeding experiment, it was discovered that the addition of 100-150 mg/kg of H. illucens larvae AMPs to the feed significantly improved the weight gain rate and specific growth rate of C. quadricarinatus. Furthermore, the addition of H. illucens larvae AMPs to the feed had no significant effect on the moisture content, crude protein, crude fat and ash content of the C. quadricarinatus muscle. The addition of 100-150 mg/kg of H. illucens larvae AMPs in the feed also increased the antioxidant capacity, nonspecific immune enzyme activity and related gene expression levels in C. quadricarinatus, thereby enhancing their antioxidant capacity and immune function. The H. illucens larvae AMPs improved the structure and composition of the intestinal microbiota of C. quadricarinatus, increasing the microbial community diversity of the crayfish gut. Finally, the addition of 100-150 mg/kg of H. illucens larvae AMPs in the feed enhanced the resistance of C. quadricarinatus against Aeromonas hydrophila, improving the survival rate of the crayfish. Based on the aforementioned findings, it is recommended that H. illucens larvae AMPs be incorporated into the C. quadricarinatus feed at a concentration of 100-150 mg/kg.
Topics: Animals; Larva; Astacoidea; Gastrointestinal Microbiome; Aeromonas hydrophila; Antimicrobial Peptides; Antioxidants; Diptera; Diet; Gene Expression; Anti-Bacterial Agents
PubMed: 38360192
DOI: 10.1016/j.fsi.2024.109437 -
Comparative Biochemistry and... Apr 2024Iron is a common and essential element for maintaining life in bacteria, plants and animals and is found in soil, fresh waters and marine waters; however, over exposure...
Iron is a common and essential element for maintaining life in bacteria, plants and animals and is found in soil, fresh waters and marine waters; however, over exposure is toxic to organisms. Iron is used in electron transport complexes within mitochondria as well as a co-factor in many essential proteins. It is also established that iron accumulation in the central nervous system in mammals is associated with various neurological disorders. Ample studies have investigated the long-term effects of iron overload in the nervous system. However, its acute effects in nervous tissue and additional organ systems warrant further studies. This study investigates the effects of iron overload on development, behavior, survival, cardiac function, and glutamatergic synaptic transmission in the Drosophila melanogaster. Additionally, physiological responses in crayfish were examined following Fe exposure. Fe reduced neuronal excitability in proprioceptive neurons in a crayfish model. Thus, Fe may block stretch activated channels (SACs) as well as voltage-gated Na channels. Exposure also rapidly reduces synaptic transmission but does not block ionotropic glutamatergic receptors, suggesting a blockage of pre-synaptic voltage-gated Ca channels in both crustacean and Drosophila models. The effects are partly reversible with acute exposure, indicating the cells are not rapidly damaged. This study is relevant in demonstrating the effects of Fe on various physiological functions in different organisms in order to further understand the acute and long-term consequences of overload.
Topics: Animals; Iron; Drosophila melanogaster; Iron Overload; Physiological Phenomena; Astacoidea; Invertebrates; Mammals
PubMed: 38354992
DOI: 10.1016/j.cbpc.2024.109856 -
Proceedings of the National Academy of... Feb 2024GABA receptor (GBR) activation inhibits neurotransmitter release in axon terminals in the brain, except in medial habenula (MHb) terminals, which show robust...
GABA receptor (GBR) activation inhibits neurotransmitter release in axon terminals in the brain, except in medial habenula (MHb) terminals, which show robust potentiation. However, mechanisms underlying this enigmatic potentiation remain elusive. Here, we report that GBR activation on MHb terminals induces an activity-dependent transition from a facilitating, tonic to a depressing, phasic neurotransmitter release mode. This transition is accompanied by a 4.1-fold increase in readily releasable vesicle pool (RRP) size and a 3.5-fold increase of docked synaptic vesicles (SVs) at the presynaptic active zone (AZ). Strikingly, the depressing phasic release exhibits looser coupling distance than the tonic release. Furthermore, the tonic and phasic release are selectively affected by deletion of synaptoporin (SPO) and Ca-dependent activator protein for secretion 2 (CAPS2), respectively. SPO modulates augmentation, the short-term plasticity associated with tonic release, and CAPS2 retains the increased RRP for initial responses in phasic response trains. The cytosolic protein CAPS2 showed a SV-associated distribution similar to the vesicular transmembrane protein SPO, and they were colocalized in the same terminals. We developed the "Flash and Freeze-fracture" method, and revealed the release of SPO-associated vesicles in both tonic and phasic modes and activity-dependent recruitment of CAPS2 to the AZ during phasic release, which lasted several minutes. Overall, these results indicate that GBR activation translocates CAPS2 to the AZ along with the fusion of CAPS2-associated SVs, contributing to persistency of the RRP increase. Thus, we identified structural and molecular mechanisms underlying tonic and phasic neurotransmitter release and their transition by GBR activation in MHb terminals.
Topics: Animals; Receptors, GABA-B; Habenula; Astacoidea; Presynaptic Terminals; Caffeine; Neurotransmitter Agents; gamma-Aminobutyric Acid
PubMed: 38346189
DOI: 10.1073/pnas.2301449121 -
Aquatic Toxicology (Amsterdam,... Mar 2024Abamectin is a globally used pesticide, which is one of 16-member macrocyclic lactones compound. As an environmental contaminant, pesticide residues pose a great threat...
Abamectin is a globally used pesticide, which is one of 16-member macrocyclic lactones compound. As an environmental contaminant, pesticide residues pose a great threat to the health and survival of aquatic animals. Procambarus clarkii is one of the most important economic aquatic animals in China. It is necessary to explore the toxic mechanism of abamectin to P. clarkii. In this study, the toxic mechanism of abamectin to P. clarkii was investigated by 0, 3 and 6 μg/L abamectin stress for 28 days. The digestive-, antioxidant- and immune- related enzymes activities, genes expression levels, and histological observations were analytical indicators of growth performance, digestive capacity, and defense systems. The results in this study showed that with abamectin concentration increasing, the growth of P. clarkii was stunted significantly, and the mortality rate increased significantly. With exposure time and abamectin concentration increasing, the expression levels of related genes, the activities of digestive-, antioxidant-, and immune- related enzymes decreased ultimately. Moreover, through histological observation, it was found that with abamectin concentration increasing, the hepatopancreas, muscle, and intestine were damaged. As elucidated by the results, once abamectin exists in the environment for a long time, even low doses will threaten to healthy growth and survival of P. clarkii. This study explored the potential toxicity and the toxic mechanism of abamectin to P. clarkii, and provides a theoretical basis for further study on the toxicity of pesticides to aquatic animals.
Topics: Animals; Antioxidants; Astacoidea; Water Pollutants, Chemical; Ivermectin; Pesticides
PubMed: 38340542
DOI: 10.1016/j.aquatox.2024.106861 -
Journal of Agricultural and Food... Feb 2024Cyantraniliprole is a novel insecticide recently introduced for rice pest control that may cause potential threats to the red swamp crayfish () in rice-crayfish...
Cyantraniliprole is a novel insecticide recently introduced for rice pest control that may cause potential threats to the red swamp crayfish () in rice-crayfish coculture systems. In this study, we investigated the acute toxicity of cyantraniliprole against with a LC value of 149.77 mg/L (96 h), first. Some abnormal behaviors of treated with 125 mg/L cyantraniliprole, including incunabular hyperexcitability, imbalance, inactivity, and increased excretion were observed. Moreover, it was observed that exposure to 5 mg/L cyantraniliprole for 14 days resulted in histopathological alterations in abdominal muscle, gills, hepatopancreas, and intestines. Furthermore, exposure to 0.05 and 5 mg/L cyantraniliprole induced increased activities of several oxidative stress-related enzymes, which was verified by the upregulation of related genes. Additionally, dysregulation of the intestinal microbiota was determined via 16S rRNA sequencing. These results will provide the basis for the utilization of cyantraniliprole in the fields of rice-crayfish integrated system.
Topics: Animals; Gastrointestinal Microbiome; Astacoidea; RNA, Ribosomal, 16S; Oxidative Stress; Oryza; Pyrazoles; ortho-Aminobenzoates
PubMed: 38324778
DOI: 10.1021/acs.jafc.3c07693 -
Comparative Biochemistry and... Mar 2024Growth is a crucial economic trait of all aquaculture species. It is important to explore the molecular regulation on growth, which could help improve the growth rate of...
Growth is a crucial economic trait of all aquaculture species. It is important to explore the molecular regulation on growth, which could help improve the growth rate of species. Mining the growth-related genes is the foundation for revealing its molecular regulation on growth. Presently, the molecular regulation of growth in Procambarus clarkii is not clear, and the study on exploring growth-related genes is limited. In this study, RNA-Seq was used to compare gene expression profiles of the individuals with different growth rates involved in four groups including Big Male (BM), Big Female (BF), Small male (SM), and Small Female (SF) from one P. clarkii family, and the analyses were performed in combination with sex. Meanwhile, whole-genome resequencing data was used to get growth-specific SNP (Single Nucleotide Polymorphism)/InDel (Insertion/Deletion) sites information. Totally, we identified 16,127 genes, of which 9065 were successfully annotated in the GO database. Among these, 1328 DEGs were identified in BM vs. SM, with 357 up-regulated and 971 down-regulated. Additionally, 3507 DEGs were identified in BF vs. SF, with 241 up-regulated and 3266 down-regulated. 96 DEGs were up-regulated and 820 DEGs were down-regulated in Growth-related Group. The expression levels of nine DEGs were validated by RT-qPCR to verify the analysis results of sequencing. 684,040 growth-related SNPs and 182,050 growth-related InDels were obtained after screened. These findings provide candidate growth-related genes and growth-specific SNP/InDel sites for regulation of growth traits in P. clarkii, and new insight into the molecular regulation of P. clarkii growth.
Topics: Humans; Female; Male; Animals; Transcriptome; Gene Expression Profiling; Astacoidea; Genome; RNA-Seq
PubMed: 38306949
DOI: 10.1016/j.cbd.2024.101198 -
Food Chemistry Jun 2024Studies have shown that high hydrostatic pressure (HHP) processing and chlorogenic acid (CA) treatment can effectively reduce food allergenicity. We hypothesize that...
Studies have shown that high hydrostatic pressure (HHP) processing and chlorogenic acid (CA) treatment can effectively reduce food allergenicity. We hypothesize that these novel processing techniques can help tackle crayfish allergy and examined the impact and mechanism of HHP (300 MPa, 15 min) and CA (CA:tropomyosin = 1:4000, 15 min) on the allergenicity of crayfish tropomyosin. Our results revealed that CA, rather than HHP, effectively reduced tropomyosin's allergenicity, as evident in the alleviation of allergic symptoms in a food allergy mouse model. Spectroscopy and molecular docking analyses demonstrated that CA could reduce the allergenicity of tropomyosin by covalent or non-covalent binding, altering its secondary structure (2.1 % decrease in α-helix; 1.9 % increase in β-fold) and masking tropomyosin's linear epitopes. Moreover, CA-treated tropomyosin potentially induced milder allergic reactions by up-regulating TLR8. While our results supported the efficacy of CA in alleviating crayfish allergy, further exploration is needed to determine clinical effectiveness.
Topics: Animals; Mice; Tropomyosin; Astacoidea; Chlorogenic Acid; Toll-Like Receptor 8; Molecular Docking Simulation; Food Hypersensitivity; Allergens
PubMed: 38301561
DOI: 10.1016/j.foodchem.2024.138614 -
Aquatic Toxicology (Amsterdam,... Mar 2024In recent years, excessive discharge of pollutants has led to increasing concentrations of cadmium (Cd) and diclofenac (DCF) in water; however, the toxicity mechanism of... (Review)
Review
Toxic effects of combined exposure to cadmium and diclofenac on freshwater crayfish (Procambarus clarkii): Insights from antioxidant enzyme activity, histopathology, and gut microbiome.
In recent years, excessive discharge of pollutants has led to increasing concentrations of cadmium (Cd) and diclofenac (DCF) in water; however, the toxicity mechanism of combined exposure of the two pollutants to aquatic animals has not been fully studied. Procambarus clarkii is an economically important aquatic species that is easily affected by Cd and DCF. This study examined the effects of combined exposure to Cd and DCF on the tissue accumulation, physiology, biochemistry, and gut microflora of P. clarkii. The results showed that Cd and DCF accumulated in tissues in the order of hepatopancreas > gill > intestine > muscle. The hepatopancreas and intestines were subjected to severe oxidative stress, with significantly increased antioxidant enzyme activity. Pathological examination revealed lumen expansion and epithelial vacuolisation in the hepatopancreas and damage to the villous capillaries and wall in the intestine. The co-exposure to Cadmium (Cd) and Diclofenac (DCF) disrupts the Firmicutes/Bacteroidetes (F/B) ratio, impairing the regular functioning of intestinal microbiota in carbon (C) and nitrogen (N) cycling. This disturbance consequently hinders the absorption and utilization of energy and nutrients in Procambarus clarkii. This study offers critical insights into the toxicological mechanisms underlying the combined effects of Cd and DCF, and suggests potential approaches to alleviate their adverse impacts on aquatic ecosystems.
Topics: Animals; Gastrointestinal Microbiome; Cadmium; Antioxidants; Diclofenac; Astacoidea; Ecosystem; Water Pollutants, Chemical; Oxidative Stress; Fresh Water; Environmental Pollutants
PubMed: 38295602
DOI: 10.1016/j.aquatox.2024.106844 -
Journal of Invertebrate Pathology Mar 2024Pathogen spores have been recognized as prey with implications for resource dynamics, energy transfer and disease transmission. In aquatic ecosystems, filter-feeders are...
Pathogen spores have been recognized as prey with implications for resource dynamics, energy transfer and disease transmission. In aquatic ecosystems, filter-feeders are able to consume such motile forms of pathogens that can cause severe disease in susceptible hosts. The interactions between European crayfish and the crayfish plague pathogen Aphanomyces astaci are of particular conservation interest. In this study, we aim to evaluate the ecological interactions between Ap. astaci, its host Astacus astacus and individuals of the genus Daphnia, filter-feeding planktonic crustaceans. Our focus was on the consumption of the motile zoospores by Daphnia individuals, but we also considered the potential of Daphnia as non-target hosts. We conducted a series of infection and life-history experiments with Ap. astaci, three Daphnia species (D. magna, D. galeata, and D. pulex) and the noble crayfish As. astacus. We did not observe any lethal effects in the infection experiments involving Ap. astaci and Daphnia. Only D. pulex showed differences in some life-history traits. The feeding experiment using the motile zoospores of Ap. astaci as alternative food source or as supplement to different amounts of algal food revealed their nutritional value: D. magna individuals survived, grew, and reproduced on a zoospore diet alone. When zoospores were supplemented to the regular algal diet, all life-history parameters have been significantly improved. However, this successful consumption of zoospores did not result in a reduced mortality of the susceptible crayfish As. astacus during the infection experiment. Nevertheless, the pathogen load of Ap. astaci in the tissues of As. astacus was significantly reduced as a consequence of the feeding activity of Daphnia. Our results indicate that an abundant filter-feeding community can reduce the amount of infective zoospores in the water body and thus be beneficial to susceptible crayfish hosts, potentially acting as a general buffer against zoospore-transmitted diseases in lentic waters.
Topics: Humans; Animals; Astacoidea; Aphanomyces; Ecosystem; Host-Pathogen Interactions; Seafood
PubMed: 38286329
DOI: 10.1016/j.jip.2024.108069