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Indian Journal of Dental Research :... Jan 2024Dental caries is a dynamic process. By using therapeutic agents, early, noncavitated lesions and caries limited to the enamel can be stopped or even remineralized. For... (Observational Study)
Observational Study
BACKGROUND
Dental caries is a dynamic process. By using therapeutic agents, early, noncavitated lesions and caries limited to the enamel can be stopped or even remineralized. For the remineralization of the initial carious lesion, many nonfluoridated remineralizing agents were investigated.
OBJECTIVES
An observational study to assess the remineralizing efficacy of tricalcium phosphate (TCP), nano-hydroxyapatite (nHAp) and ozone remineralizing agents on the artificial carious lesion.
METHODOLOGY
In this observational research, the artificial carious lesion was produced on extracted 40 premolar teeth. Later, remineralizing agents (Group A: nHAp, Group B: TCP, Group C: Ozone remineralizing agents, Group D: Control group (Deionized water) were used to remineralize demineralized teeth. Utilizing the Vickers Hardness Number, the level of demineralization and remineralization was assessed. Later these readings were statistically assessed using the Tukey's HSD (honestly significant difference) and ANOVA tests in SPSS version 21.0. The P value was set at 0.05 or less.
RESULTS
After demineralization, there was a decrease in enamel microhardness values, with 32% in Group A, 26% in Group B, 22% in Group C, and 21% in Group D, respectively. From the baseline to demineralization, there was a statistically significant decrease in microhardness across all groups. After remineralization, Groups A, B, and C experienced an increase in microhardness while Group D experienced no changes. This showed that Group A had the highest remineralization percentage, followed by Group B and Group C.
CONCLUSION
nHAp and TCP had the greater remineralizing ability, which can be used to manage initial carious lesions.
Topics: Calcium Phosphates; Tooth Remineralization; Durapatite; Dental Caries; Humans; Ozone; In Vitro Techniques; Cariostatic Agents; Bicuspid; Dental Enamel
PubMed: 38934756
DOI: 10.4103/ijdr.ijdr_704_22 -
International Journal of Molecular... Jun 2024Zr-50Ti alloys are promising biomaterials due to their excellent mechanical properties and low magnetic susceptibility. However, Zr-50Ti alloys do not inherently bond...
Zr-50Ti alloys are promising biomaterials due to their excellent mechanical properties and low magnetic susceptibility. However, Zr-50Ti alloys do not inherently bond well with bone. This study aims to enhance the bioactivity and bonding strength of Zr-50Ti alloys for orthopedic implant materials. Initially, the surface of Zr-50Ti alloys was treated with a sulfuric acid solution to create a microporous structure, increasing surface roughness and area. Subsequently, low crystalline calcium phosphate (L-CaP) precipitation was controlled by adding Mg and/or CO ions in modified simulated body fluid (m-SBF). The treated Zr-50Ti alloys were then subjected to cold isostatic pressing to force m-SBF into the micropores, followed by incubation to allow L-CaP formation. The apatite-forming process was tested in simulated body fluid (SBF). The results demonstrated that the incorporation of Mg and/or CO ions enabled the L-CaP to cover the entire surface of Zr-50Ti alloys within only one day. After short-term soaking in SBF, the L-CaP layer, modulated by Mg and/or CO ions, formed a uniform hydroxyapatite (HA) coating on the surface of the Zr-50Ti alloys, showing potential for optimized bone integration. After soaking in SBF for 14 days, the bonding strength between the apatite layer and alloy has the potential to meet the orthopedic application requirement of 22 MPa. This study demonstrates an effective method to enhance the bioactivity and bonding strength of Zr-50Ti alloys for orthopedic applications.
Topics: Alloys; Zirconium; Body Fluids; Calcium Phosphates; Surface Properties; Titanium; Biocompatible Materials; Materials Testing; Magnesium; Durapatite
PubMed: 38928293
DOI: 10.3390/ijms25126587 -
International Journal of Molecular... Jun 2024Polyurethane (PU) is a promising material for addressing challenges in bone grafting. This study was designed to enhance the bone grafting capabilities of PU by...
Polyurethane (PU) is a promising material for addressing challenges in bone grafting. This study was designed to enhance the bone grafting capabilities of PU by integrating hydroxyapatite (HAp), which is known for its osteoconductive and osteoinductive potential. Moreover, a uniform distribution of HAp in the porous structure of PU increased the effectiveness of bone grafts. PEG/APTES-modified scaffolds were prepared through self-foaming reactions. A uniform pore structure was generated during the spontaneous foaming reaction, and HAp was uniformly distributed in the PU structure (PU15HAp and PU30HAp) during foaming. Compared with the PU scaffolds, the HAp-modified PU scaffolds exhibited significantly greater protein absorption. Importantly, the effect of the HAp-modified PU scaffold on bone repair was tested in a rat calvarial defect model. The microstructure of the newly formed bone was analyzed with microcomputed tomography (μ-CT). Bone regeneration at the defect site was significantly greater in the HAp-modified PU scaffold group than in the PU group. This innovative HAp-modified PU scaffold improves current bone graft materials, providing a promising avenue for improved bone regeneration.
Topics: Polyurethanes; Animals; Durapatite; Tissue Scaffolds; Rats; Bone Regeneration; Skull; Rats, Sprague-Dawley; X-Ray Microtomography; Male; Porosity; Bone Transplantation
PubMed: 38928145
DOI: 10.3390/ijms25126440 -
Scientific Reports Jun 2024The aim of this study is to introduce a dental capping agent for the treatment of pulp inflammation (pulpitis). Nanohydroxyapatite with Elaeagnus angustifolia L. extract...
The aim of this study is to introduce a dental capping agent for the treatment of pulp inflammation (pulpitis). Nanohydroxyapatite with Elaeagnus angustifolia L. extract (nHAEA) loaded with metronidazole (nHAEA@MTZ) was synthesized and evaluated using a lipopolysaccharide (LPS) in vitro model of pulpitis. nHAEA was synthesized through sol-gel method and analyzed using Scanning Electron Microscopy, Transmission Electron Microscopy, and Brunauer Emmett Teller. Inflammation in human dental pulp stem cells (HDPSCs) induced by LPS. A scratch test assessed cell migration, RT PCR measured cytokines levels, and Alizarin red staining quantified odontogenesis. The nHAEA nanorods were 17-23 nm wide and 93-146 nm length, with an average pore diameter of 27/312 nm, and a surface area of 210.89 m/g. MTZ loading content with controlled release, suggesting suitability for therapeutic applications. nHAEA@MTZ did not affect the odontogenic abilities of HDPSCs more than nHAEA. However, it was observed that nHAEA@MTZ demonstrated a more pronounced anti-inflammatory effect. HDPSCs treated with nanoparticles exhibited improved migration compared to other groups. These findings demonstrated that nHAEA@MTZ could be an effective material for pulp capping and may be more effective than nHAEA in reducing inflammation and activating HDPSCs to enhance pulp repair after pulp damage.
Topics: Plant Extracts; Humans; Pulpitis; Metronidazole; Dental Pulp; Durapatite; Nanoparticles; Green Chemistry Technology; Drug Carriers; Stem Cells; Cell Movement; Cells, Cultured
PubMed: 38926433
DOI: 10.1038/s41598-024-65582-4 -
Journal of Materials Science. Materials... Jun 2024The current clinical application of glaucoma drainage devices is made of non-degradable materials. These non-degradable drainage devices often trigger inflammatory...
The current clinical application of glaucoma drainage devices is made of non-degradable materials. These non-degradable drainage devices often trigger inflammatory responses and scar proliferation, possibly leading to surgical failure. We developed a biodegradable material hydroxyapatite-coated magnesium (HA-Mg) as a glaucoma drainage device. Twelve New Zealand white rabbits were randomly assigned to three groups: HA-Mg drainage plate group (6 right eyes), trabeculectomy group (6 right eyes), and control group (12 left eyes). Results showed that all HA-Mg drainage plates were completely degraded ~4 months postoperatively. At the 5th month postoperatively, there was no statistical difference in the corneal endothelium density between the HA-Mg drainage plate group and the control group (p = 0.857). The intraocular pressure (IOP) level in the HA-Mg drainage plate implantation group was lower than in the other two groups. The trypan blue dye still drained from the anterior chamber to the subconjunctiva 5 months after HA-Mg drainage plate implantation. HE staining revealed the scleral linear aqueous humor drainage channel and anterior synechia were observed after drainage plate completely degraded, with no obvious infiltration with the inflammatory cells. This study showed the safety and efficacy of HA-Mg glaucoma drainage plate in controlling IOP after implantation into the anterior chamber of rabbit eyes.
Topics: Animals; Rabbits; Intraocular Pressure; Glaucoma Drainage Implants; Anterior Chamber; Glaucoma; Magnesium; Durapatite; Trabeculectomy
PubMed: 38916635
DOI: 10.1007/s10856-024-06806-x -
F1000Research 2023A non-union fracture is one of the most common complications arising from an untreated fracture. Bone grafts are able to fasten bone healing which can prevent and cure...
A non-union fracture is one of the most common complications arising from an untreated fracture. Bone grafts are able to fasten bone healing which can prevent and cure non-union fractures. Therefore, alternative hydroxyapatite bone grafts from waste resources are needed to increase the availability of bone grafts in the healthcare system. A bone substitute, hydroxyapatite (HA), has the ability to prevent non-union fractures. Green mussel shell contains 95.69 percent HA, allowing for an annual production of 133.97-287.07 tons per ha of HA, and is a potent alternative material in the manufacture of HA. This research was conducted for four months using a true experimental research method with a post-test-only control group design. This study used 36 New Zealand rabbits ( ) which were divided into 9 groups: positive control, negative control, and intervention at weeks 2, 4 and 6 after the intervention. All groups were subjected to three general procedures: pre-surgery, surgery, and post-surgery. The findings demonstrated that green mussel shell HA has efficacy in accelerating bone healing, better than HA bovine, as compared to the 6-week negative control group and demonstrated a significant difference ( < 0.05). Green mussel hydroxyapatite is proven to be able to fasten and maximize the bone healing process as fast as bovine HA, and even has higher efficacy than bovine HA.
Topics: Animals; Durapatite; Rabbits; Femur; Perna; Alkaline Phosphatase; Animal Shells; Bone Substitutes
PubMed: 38915771
DOI: 10.12688/f1000research.132881.1 -
Journal of Applied Biomaterials &... 2024This study aims to investigate the effect of coating time on the formation of hydroxyapatite (HA) coating layer on ZK60 substrate and understand the biodegradation...
OBJECTIVES
This study aims to investigate the effect of coating time on the formation of hydroxyapatite (HA) coating layer on ZK60 substrate and understand the biodegradation behavior of the coated alloy for biodegradable implant applications.
METHODS
Biodegradable ZK60 alloy was coated by HA layer for different times of 0.5, 1, 2, and 4 h by chemical conversion method. After coating, all the coated specimens were used for immersion test in Hanks' solution to understand the effect of coating time on the degradation behavior of the alloy. The degradation rate of the coated alloy was evaluated by Mg ion quantification and pH change during immersion test. The microstructure of the coating layer was examined by scanning electron microscope (SEM) equipped with an energy-dispersive X-ray spectroscopy (EDS) before and after immersion to understand the degradation behavior of the coated alloy.
RESULTS
HA coating layers were formed successfully on surface of ZK60 specimens after 0.5, 1, 2, and 4 h with different microstructure. Optimal coating quality was observed at 1 or 2 h, characterized by well-formed and uniform HA layers. However, extending the coating duration to 4 h led to the formation of cracks within the HA layer, accompanied by Mg(OH). Specimens coated for 1 and 2 h exhibited the lowest degradation rates, while specimens coated for 0.5 and 4 h showed the highest degradation rates. Furthermore, analysis of degradation products revealed the predominance of calcium phosphates formed on the surface of specimens coated for 1 and 2 h. Conversely, specimens coated for 0.5 and 4 h exhibited Mg(OH) as the primary degradation product, suggesting a less effective corrosion barrier under these conditions.
CONCLUSION
The HA layer formed after 2 h demonstrated as the most effective coating layer for enhancing the corrosion resistance of the ZK60 alloy for biomedical applications.
Topics: Durapatite; Alloys; Coated Materials, Biocompatible; Materials Testing; Corrosion; Magnesium
PubMed: 38912599
DOI: 10.1177/22808000241251564 -
Journal of Materials Science. Materials... Jun 2024Calcium phosphate cements, primarily brushite cements, require the addition of setting retarders to ensure adequate processing time and processability. So far, citric...
Calcium phosphate cements, primarily brushite cements, require the addition of setting retarders to ensure adequate processing time and processability. So far, citric acid has been the primary setting retarder used in this context. Due to the poor biocompatibility, it is crucial to explore alternative options for better processing. In recent years, the setting retarder phytic acid (IP6) has been increasingly investigated. This study investigates the biological behaviour of calcium phosphate cements with varying concentrations of IP6, in addition to their physical properties. Therefore cytocompatibility in vitro testing was performed using osteoblastic (MG-63) and osteoclastic (RAW 264.7 differentiated with RANKL) cells. We could demonstrate that the physical properties like the compressive strength of specimens formed with IP6 (brushite_IP6_5 = 11.2 MPa) were improved compared to the reference (brushite = 9.8 MPa). In osteoblast and osteoclast assays, IP6 exhibited significantly better cytocompatibility in terms of cell activity and cell number for brushite cements up to 11 times compared to the brushite reference. In contrast, the calcium-deficient hydroxyapatite (CDHA) cements produced similar results for IP6 (CDHA_IP6_0.25 = 27.0 MPa) when compared to their reference (CDHA = 21.2 MPa). Interestingly, lower doses of IP6 were found to be more effective than higher doses with up to 3 times higher. Additionally, IP6 significantly increased degradation in both passive and active resorption. For these reasons, IP6 is emerging as a strong new competitor to established setting retarders such as citric acid. These cements have potential applications in bone augmentation, the stabilisation of non-load bearing fractures (craniofacial), or the cementation of metal implants.
Topics: Phytic Acid; Animals; Calcium Phosphates; Mice; Materials Testing; Bone Cements; Osteoblasts; RAW 264.7 Cells; Humans; Osteoclasts; Compressive Strength; Biocompatible Materials; Durapatite
PubMed: 38900219
DOI: 10.1007/s10856-024-06805-y -
Molecules (Basel, Switzerland) May 2024Bone tissue engineering (BTE) is the most promising strategy to repair bones injuries and defects. It relies on the utilization of a temporary support to host the cells...
Bone tissue engineering (BTE) is the most promising strategy to repair bones injuries and defects. It relies on the utilization of a temporary support to host the cells and promote nutrient exchange (i.e., the scaffold). Supercritical CO assisted drying can preserve scaffold nanostructure, crucial for cell attachment and proliferation. In this work, agarose aerogels, loaded with hydroxyapatite were produced in view of BTE applications. Different combinations of agarose concentration and hydroxyapatite loadings were tested. FESEM and EDX analyses showed that scaffold structure suffered from partial closure when increasing filler concentration; hydroxyapatite distribution was homogenous, and Young's modulus improved. Looking at BTE applications, the optimal combination of agarose and hydroxyapatite resulted to be 1% / and 10% /, respectively. Mechanical properties showed that the produced composites could be eligible as starting scaffold for BTE, with a Young's Modulus larger than 100 kPa for every blend.
Topics: Sepharose; Tissue Engineering; Durapatite; Tissue Scaffolds; Bone and Bones; Elastic Modulus; Gels; Humans; Materials Testing; Biocompatible Materials
PubMed: 38893374
DOI: 10.3390/molecules29112498 -
BMC Oral Health Jun 2024This study evaluated the clinical benefits of adding NanoBone with split-crest technique and simultaneous implant placement covered with platelet-rich fibrin membrane in... (Randomized Controlled Trial)
Randomized Controlled Trial Comparative Study
Tomographic assessment of bone changes in atrophic maxilla treated by split-crest technique and dental implants with platelet-rich fibrin and NanoBone versus platelet-rich fibrin alone: Randomized controlled trial.
BACKGROUND
This study evaluated the clinical benefits of adding NanoBone with split-crest technique and simultaneous implant placement covered with platelet-rich fibrin membrane in horizontally deficient maxillary ridges in terms of crestal and horizontal bone changes and patient morbidity.
METHODS
Forty patients indicated for maxillary ridge splitting and simultaneous implant placement were assigned randomly to the study groups: control group (Platelet Rich Fibrin membrane) and test group (Platelet Rich Fibrin membrane + Nanobone). The Cone Beam Computed Tomography Fusion technique was utilized to assess crestal and horizontal bone changes after five months of the surgical procedure. Patient morbidity was recorded for one week post-surgical.
RESULTS
Five months post-surgical, buccal crestal bone resorption was 1.26 ± 0.58 mm for the control group and 1.14 ± 0.63 mm for the test group. Lingual crestal bone resorption was 1.40 ± 0.66 mm for the control group and 1.47 ± 0.68 mm for the test group. Horizontal bone width gain was 1.46 ± 0.44 mm for the control group and 1.29 ± 0.73 mm for the test group. There was no significant statistical difference between study groups regarding crestal and horizontal bone changes and patient morbidity.
CONCLUSIONS
The tomographic assessment of NanoBone addition in this study resulted in no statistically significant difference between study groups regarding crestal and horizontal bone changes and patient morbidity. More randomized controlled clinical trials on gap fill comparing different bone grafting materials versus no grafting should be conducted.
GOV REGISTRATION NUMBER
NCT02836678, 13 January 2017.
Topics: Humans; Platelet-Rich Fibrin; Male; Female; Cone-Beam Computed Tomography; Maxilla; Middle Aged; Alveolar Bone Loss; Dental Implants; Adult; Alveolar Ridge Augmentation; Dental Implantation, Endosseous; Aged; Minerals; Follow-Up Studies; Drug Combinations; Silicon Dioxide; Durapatite
PubMed: 38877464
DOI: 10.1186/s12903-024-04420-5