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Sensors (Basel, Switzerland) Jan 2023In order to advance engineering applications of robotics such as wearable health-monitoring devices, humanoid robots, etc., it is essential to investigate the tactile...
In order to advance engineering applications of robotics such as wearable health-monitoring devices, humanoid robots, etc., it is essential to investigate the tactile sensations of artificial haptic sensors mimicking bioinspired human cutaneous mechanoreceptors such as free nerve endings, Merkel's cells, Krause end bulbs, Meissner corpuscles, Ruffini endings, and Pacinian corpuscles. The generated receptor's potential response to extraneous stimuli, categorized as slow adaption (SA) or fast adaption (FA), is particularly significant as a typical property. The present study addressed the estimation of SA and FA by utilizing morphologically fabricated mechanoreceptors made of our proposed magnetically responsive intelligent fluid, hybrid fluid (HF), and by applying our proposed electrolytic polymerization. Electric circuit models of the mechanoreceptors were generated using experimental data on capacitance and inductance on the basis of the electric characteristics of impedance. The present results regarding equivalent firing rates based on FA and SA are consistent with the FA and SA findings of vital mechanoreceptors by biomedical analysis. The present investigative process is useful to clarify the time of response to a force on the fabricated artificial mechanoreceptor.
PubMed: 36772367
DOI: 10.3390/s23031327 -
Somatosensory & Motor Research Mar 2024The purpose of this study was to determine the changes in the Blood Oxygen Level Dependent signal of Primary somatosensory area (S1) and Brodmann area 3 (BA3) per finger...
PURPOSE AND METHOD
The purpose of this study was to determine the changes in the Blood Oxygen Level Dependent signal of Primary somatosensory area (S1) and Brodmann area 3 (BA3) per finger and phalanx in comparison to the activation voxel when 250 Hz vibratory stimulation with high sensitivity for the Pacinian corpuscle was given to the four fingers and three phalanges.
RESULTS
The result of analyzing the activation voxel showed a significant difference for S1 per finger and phalanx, but for BA3, no significant difference was observed despite a similar trend to S1. In contrast, the activation intensity (BOLD) displayed a significant difference for S1 per finger and phalanx and for BA3, where the activation voxel had no significant variation. In addition, while the result of S1 did not indicate whether the index or the little fingers had the highest sensitivity based on the BOLD signal per finger, the result of BA3 marked the strongest BOLD signal for the little finger as a response to 250 Hz vibratory stimulation. The activation intensity per phalanx was the highest for the intermediate phalanx for S1 and BA3, which was in line with a previous study comparing the activation voxel.
CONCLUSIONS
The method based on the intensity of the nerve activation is presumed to have high sensitivity as the signal intensity is monitored within a specific, defined area. Thus, for the extraction of brain activation patterns of micro-domains, such as BA3, monitoring the BOLD signal that reflects the nerve activation intensity more sensitively is likely to be advantageous.
Topics: Somatosensory Cortex; Magnetic Resonance Imaging; Fingers; Brain Mapping
PubMed: 36721377
DOI: 10.1080/08990220.2023.2173165 -
Tomography (Ann Arbor, Mich.) Sep 2022Anatomically accurate models of a human finger can be useful in simulating various disorders. In order to have potential clinical value, such models need to include a...
Anatomically accurate models of a human finger can be useful in simulating various disorders. In order to have potential clinical value, such models need to include a large number of tissue types, identified by an experienced professional, and should be versatile enough to be readily tailored to specific pathologies. Magnetic resonance images were acquired at ultrahigh magnetic field (7 T) with a radio-frequency coil specially designed for finger imaging. Segmentation was carried out under the supervision of an experienced radiologist to accurately capture various tissue types (TTs). The final segmented model of the human index finger had a spatial resolution of 0.2 mm and included 6,809,600 voxels. In total, 15 TTs were identified: subcutis, Pacinian corpuscle, nerve, vein, artery, tendon, collateral ligament, volar plate, pulley A4, bone, cartilage, synovial cavity, joint capsule, epidermis and dermis. The model was applied to the conditions of arthritic joint, ruptured tendon and variations in the geometry of a finger. High-resolution magnetic resonance images along with careful segmentation proved useful in the construction of an anatomically accurate model of the human index finger. An example illustrating the utility of the model in biomedical applications is shown. As the model includes a number of tissue types, it may present a solid foundation for future simulations of various musculoskeletal disease processes in human joints.
Topics: Humans; Magnetic Resonance Imaging; Fingers; Radio Waves; Tendons; Tendon Injuries
PubMed: 36287795
DOI: 10.3390/tomography8050196 -
Proceedings of the National Academy of... Oct 2022Low-threshold mechanoreceptors (LTMRs) and their cutaneous end organs convert light mechanical forces acting on the skin into electrical signals that propagate to the...
Low-threshold mechanoreceptors (LTMRs) and their cutaneous end organs convert light mechanical forces acting on the skin into electrical signals that propagate to the central nervous system. In mouse hairy skin, hair follicle-associated longitudinal lanceolate complexes, which are end organs comprising LTMR axonal endings that intimately associate with terminal Schwann cell (TSC) processes, mediate LTMR responses to hair deflection and skin indentation. Here, we characterized developmental steps leading to the formation of Aβ rapidly adapting (RA)-LTMR and Aδ-LTMR lanceolate complexes. During early postnatal development, Aβ RA-LTMRs and Aδ-LTMRs extend and prune cutaneous axonal branches in close association with nascent TSC processes. Netrin-G1 is expressed in these developing Aβ RA-LTMR and Aδ-LTMR lanceolate endings, and ablation experiments indicate that Netrin-G1 functions in sensory neurons to promote lanceolate ending elaboration around hair follicles. The Netrin-G ligand (NGL-1), encoded by , is expressed in TSCs, and ablation of partially phenocopied the lanceolate complex deficits observed in mutants. Moreover, NGL-1-Netrin-G1 signaling is a general mediator of LTMR end organ formation across diverse tissue types demonstrated by the fact that Aβ RA-LTMR endings associated with Meissner corpuscles and Pacinian corpuscles are also compromised in the and mutant mice. Thus, axon-glia interactions, mediated in part by NGL-1-Netrin-G1 signaling, promote LTMR end organ formation.
Topics: Animals; Mice; Axons; Ligands; Mechanoreceptors; Netrins; Schwann Cells; Skin
PubMed: 36252008
DOI: 10.1073/pnas.2210421119 -
Biomedical Research (Tokyo, Japan) 2022Peripheral nerves are provided with a blood-nerve barrier which prevents the invasion of harmful substances and pathogens, and also regulates metabolic and ionic... (Review)
Review
Peripheral nerves are provided with a blood-nerve barrier which prevents the invasion of harmful substances and pathogens, and also regulates metabolic and ionic homeostasis within nerve fascicles. The barrier functions are attributed to both the concentric layer of flattened cells in the perineurium and blood vessels running in the endoneurium. The perineurial cells develop continuous tight junctions as a diffusion barrier. In order to take up a predominant nutrient, glucose, the perineurium as well as endoneurial capillaries expresses GLUT1, a glucose transporter. An axon-Schwann cell complex within peripheral nerves utilizes glucose as a major energy source via the GLUT1, as does the brain. Under conditions of a reduced utilization of glucose, only the perineurial cells can transfer other nutrients, namely monocarboxylates such as ketone bodies and lactate via MCT1. Thus, MCT1 colocalizes with GLUT1 in the perineurium but not in endoneurial capillaries. To identify the cellular origins of the nerve sheath, marker proteins such as glial specific S100 protein, GLUT1, endoneurial CD34, and EMA (epithelial membrane antigen) are useful. Immunohistochemical findings for these markers are reviewed in this paper, focusing on the perineurium and endoneurium and their derivatives, Pacinian and Meissner corpuscles. Growing evidence throws light on the critical involvement of the nerve sheaths in the development, maintenance, and diseases of peripheral nerves.
Topics: Glucose; Glucose Transporter Type 1; Ketone Bodies; Lactates; Mucin-1; Peripheral Nerves; S100 Proteins
PubMed: 36244793
DOI: 10.2220/biomedres.43.145 -
Sensors (Basel, Switzerland) Sep 2022The development of gustatory sensors is essential for the development of smart materials for use in robotics, and in the food, beverage, and pharmaceutical industries....
The development of gustatory sensors is essential for the development of smart materials for use in robotics, and in the food, beverage, and pharmaceutical industries. We therefore designed a prototype of a rubber tongue embedded with a gustatory receptor mimicking a human tongue using our previously proposed hybrid fluid rubber (HF rubber) and an electrolytic polymerization technique. The fabricated gustatory receptor was composed of Pacinian corpuscles, which are well known and have already been elucidated as effective haptic and auditory receptors in previous studies. Moreover, the receptor has self-powered voltage generated as built-in electricity as a result of the ionized particles and molecules in the HF rubber. The utilization of a layered structure for the Pacinian corpuscles induced a typical response not only to normal and shear forces but to thermal variations. Typical gustatory characteristics, including the initial response voltage and the cyclic voltammogram form, were clearly varied by five tastes: saltiness, sourness, sweetness, bitterness, and umami. These results were due to ORP, pH, and conductivity.
Topics: Electrolytes; Humans; Polymerization; Rubber; Smart Materials; Taste; Tongue
PubMed: 36146328
DOI: 10.3390/s22186979 -
Sensors (Basel, Switzerland) Jul 2022The development of auditory sensors and systems is essential in smart materials of robotics and is placed at the strategic category of mutual communication between...
The development of auditory sensors and systems is essential in smart materials of robotics and is placed at the strategic category of mutual communication between humans and robots. We designed prototypes of the rubber-made equilibrium and auditory sensors, mimicking hair cells in the saccule and the cochlea at the vestibule of the human ear by utilizing our previously proposed technique of electrolytic polymerization on the hybrid fluid rubber (HF rubber). The fabricated artificial hair cells embedded with mimicked free nerve endings and Pacinian corpuscles, which are well-known receptors in the human skin and have already been elucidated effective in the previous study, have the intelligence of equilibrium and auditory sensing. Moreover, they have a voltage that is generated from built-in electricity caused by the ionized particles and molecules in the HF rubber due to piezoelectricity. We verified the equilibrium and auditory characteristics by measuring the changes in voltage with inclination, vibration over a wide frequency range, and sound waves. We elucidated experimentally that the intelligence has optimum morphological conditions. This work has the possibility of advancing the novel technology of state-of-the-art social robotics.
Topics: Humans; Polymerization; Robotics; Rubber; Skin, Artificial; Smart Materials
PubMed: 35891135
DOI: 10.3390/s22145447 -
Diagnostics (Basel, Switzerland) Jul 2022Plantar fibromatosis, known as Ledderhose disease, is a neoplastic disease characterized by a locally-aggressive bland fibroblastic proliferation. Although Pacinian...
BACKGROUND
Plantar fibromatosis, known as Ledderhose disease, is a neoplastic disease characterized by a locally-aggressive bland fibroblastic proliferation. Although Pacinian corpuscles alterations are commonly described in palmar fibromatosis, there are still no references about Pacinian corpuscles alterations in the rarer plantar version.
METHODS
We present a case report where a wide cutaneous resection, including the plantar fascia was performed, allowing a detailed study of Pacinian corpuscles. Pacinian corpuscles were analyzed using immunohistochemistry for neurofilament proteins, S100 protein, CD34, vimentin, glucose transporter 1, epithelial membrane antigen, neural-cell adhesion molecule, actin, desmin, type IV collagen, and high-affinity neurotrophin Trk-receptors. Moreover, the density and the size of the corpuscles were determined.
RESULTS
A clear increase in the number (hyperplasia) of Pacinian corpuscles was evidenced in the Ledderhose disease plantar fascia in comparison with similarly aged normal subjects. Pacinian hypertrophy was not demonstrated, but a significant decrease in the number of corpuscular lamellae was noted, with a subsequent increase in the interlamellar spaces. Pacinian corpuscles from the pathological plantar fascia showed an abnormal structure and immunohistochemical profile, generally without identifiable axons, and also absence of an inner core or an intermediate layer. Moreover, other molecules related with trophic maintenance of corpuscles were also absent. Finally, a vascular proliferation was commonly noted in some corpuscles, which involved all corpuscular constituents.
CONCLUSION
The observed Pacinian corpuscles hyperplasia could be considered a diagnostic clue of plantar fibromatosis.
PubMed: 35885610
DOI: 10.3390/diagnostics12071705 -
Annals of Anatomy = Anatomischer... Aug 2022Small clear synaptic-like vesicles fill axon terminals of mechanoreceptors. Their functional significance is controversial and probably includes release of...
BACKGROUND
Small clear synaptic-like vesicles fill axon terminals of mechanoreceptors. Their functional significance is controversial and probably includes release of neurotransmitters from afferent axon terminals. Synaptophysin, a major protein of the synaptic vesicle membrane, is present in presynaptic endings of the central and peripheral nervous systems. It is also expressed in mechanosensory neurons which extend into skin forming sensory corpuscles. Nevertheless, synaptophysin occurrence in these structures has never been investigated.
METHODS
Here we used immunohistochemistry to detect synaptophysin in adult human dorsal root ganglia, cutaneous Meissner and Pacinian corpuscles and Merkel cell-neurite complexes from foetal to elderly period. Moreover, we analyzed whether synaptophysin co-localizes with the mechano-gated protein PIEZO2.
RESULTS
Synaptophysin immunoreactivity was observed in primary sensory neurons (36 ± 6%) covering the entire soma size ranges. Axons of Meissner's and Pacinian corpuscles were positive for synaptophysin from 36 and 12 weeks of estimated gestational age respectively, to 72 years old. Synaptophysin was also detected in Merkel cells (from 14 weeks of estimated gestational age to old age). Additionally in adult skin, synaptophysin and PIEZO2 co-localized in the axon of Meissner and Pacinian corpuscles, Merkel cells as well as in some axons of Merkel cell-neurite complexes.
CONCLUSION
Present results demonstrate that a subpopulation of primary sensory neurons and their axon terminals forming cutaneous sensory corpuscles contain synaptophysin, a typical presynaptic vesicle protein. Although the functional relevance of these findings is unknown it might be related to neurotransmission mechanisms linked to mechanotransduction.
Topics: Adult; Aged; Axons; Biomarkers; Humans; Mechanoreceptors; Mechanotransduction, Cellular; Pacinian Corpuscles; Skin; Synaptophysin
PubMed: 35588932
DOI: 10.1016/j.aanat.2022.151955 -
Annals of Anatomy = Anatomischer... Aug 2022PIEZO2 is a transmembrane protein forming part of an ion channel required for mechanotransduction. In humans, PIEZO2 is present in axon terminals of adult Meissner and...
BACKGROUND
PIEZO2 is a transmembrane protein forming part of an ion channel required for mechanotransduction. In humans, PIEZO2 is present in axon terminals of adult Meissner and Pacinian corpuscles, as well as Merkel cells in Merkel cell-neurite complexes.
METHODS
To study the acquisition of functional capability for mechanotransduction of developing type I slowly adapting low-threshold mechanoreceptors, i.e., Merkel cell-neurite complexes, a battery of immunohistochemical and immunofluorescence techniques was performed on human skin specimens covering the whole development and growth, from 11 weeks of estimated gestational age to 20 years of life. In addition, developmental expression of PIEZO2 type I (Meissner's corpuscles) and type II (Pacinian corpuscles) rapidly adapting mechanoreceptors was studied in parallel.
RESULTS
The first evidence of Merkel cells showing the typical morphology and placement was at 13 weeks of estimated gestation age, and at this time positive immunoreactivity for PIEZO2 was achieved. PIEZO2 expression in axons terminals started at 23 WEGA in Pacinian corpuscles and at 36 WEGA in the case of Meissner corpuscles. The occurrence of PIEZO2 in Merkel cells, Meissner and Pacinian corpuscles was maintained for all the time investigated. Interestingly PIEZO2 was absent in most Aβ type I slowly adapting low-threshold mechanoreceptors that innervate MC while it was regularly present in most Aβ type I and type II rapidly adapting low-threshold mechanoreceptors that supplies Meissner and Pacinian corpuscles.
CONCLUSION
The present results provide evidence that human cutaneous mechanoreceptors could perform mechanotransduction already during embryonic development.
Topics: Adult; Female; Humans; Ion Channels; Mechanoreceptors; Mechanotransduction, Cellular; Merkel Cells; Pacinian Corpuscles; Pregnancy; Skin
PubMed: 35523396
DOI: 10.1016/j.aanat.2022.151953