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JPEN. Journal of Parenteral and Enteral... Sep 2023Our goal was to quantify the pH and total acidity of human milk fortified with human milk fortifiers (HMFs), powder infant formulas, and protein additives.
BACKGROUND
Our goal was to quantify the pH and total acidity of human milk fortified with human milk fortifiers (HMFs), powder infant formulas, and protein additives.
METHODS
Commercial liquid HMFs and powder infant formulas were added to pasteurized pooled donor human milk in triplicate and stirred. The pH of unfortified and fortified human milk at 22, 24, 26, 27, 28, and 30 kcal/ounce (624, 680, 737, 765, 794, and 850 kcal/g, respectively) was determined using a pH meter. Phenolphthalein acidity at 24 and 30 kcal/ounce (680 and 850 kcal/g, respectively) was determined using diluted sodium hydroxide.
RESULTS
The pH of unfortified human milk increased within the first hour (6.52 ± 0.06 vs 6.62 ± 0.05, P < 0.0001). Changes in pH largely correlated with caloric density; however, directional changes varied considerably between HMFs and powder infant formulas. Two liquid HMFs demonstrated modest reductions in pH with increasing caloric density whereas one liquid HMF alkalinized human milk with increasing caloric density (analysis of variance P < 0.0001). Phenolphthalein acidity was significantly higher for five HMFs and lower for one HMF at 30 kcal/ounce (850 kcal/g) but not 24 kcal/ounce (680 kcal/g). Powder infant formulas generally increased pH with increasing caloric density (analysis of variance P < 0.0001), but no differences in phenolphthalein acidity were noted.
CONCLUSION
Changes in acid/base balancefor fortified human milk are variable and may be a consideration when selecting a fortifying agent for human milk.
Topics: Infant; Humans; Milk, Human; Powders; Food, Fortified; Dietary Supplements; Phenolphthaleins
PubMed: 37350060
DOI: 10.1002/jpen.2537 -
BMC Musculoskeletal Disorders Jun 2023This study aims to investigate the effect of a course of selected corrective exercises on posture, scapula-humeral rhythm and performance of adolescent volleyball...
BACKGROUND
This study aims to investigate the effect of a course of selected corrective exercises on posture, scapula-humeral rhythm and performance of adolescent volleyball players.
METHODS
30 adolescent volleyball players with upper cross syndrome were purposefully selected and assigned into 2 control and training groups. The degree of back curvature was evaluated using a flexible ruler, forward head and forward shoulder size by photographic method, scapula-humeral rhythm by Lateral Scapular Slide Test (LSST), and performance by closed kinetic chain test. The training group performed the exercises for 10 weeks. After the exercises, the post-test was administered. To analyze the data, analysis of co-variance tests and paired t-test at the level of 0.05 were employed.
RESULTS
The research results showed that corrective exercises have a significant effect on abnormalities of forward head, forward shoulder, kyphosis, scapula-humeral rhythm and performance.
CONCLUSIONS
Corrective exercises can be effective in reducing shoulder girdle and spine abnormalities and improving scapula- humeral rhythm and performance of volleyball players.
Topics: Adolescent; Humans; Volleyball; Humerus; Scapula; Posture; Dioctyl Sulfosuccinic Acid; Phenolphthalein
PubMed: 37316911
DOI: 10.1186/s12891-023-06592-7 -
Biosensors May 2023Osteoarthritis (OA) is the most common joint disease, which accompanies pain and inconvenience in daily life owing to degradation of cartilage and adjacent tissues. In...
Osteoarthritis (OA) is the most common joint disease, which accompanies pain and inconvenience in daily life owing to degradation of cartilage and adjacent tissues. In this study, we propose a simple point-of-care testing (POCT) kit for the detection of the MTF1 OA biomarker to achieve on-site clinical diagnosis of OA. The kit contains an FTA card for patient sample treatments, a sample tube for loop-mediated isothermal amplification (LAMP), and a phenolphthalein-soaked swab for naked eye detection. The MTF1 gene was isolated from synovial fluids using an FTA card and amplified using the LAMP method at 65 °C for 35 min. A test part of the phenolphthalein-soaked swab was decolorized in the presence of the MTF1 gene due to the pH change after the LAMP, but the color remained pink in the absence of the MTF1 gene. The control part of the swab served as a reference color in relation to the test part. When real-time LAMP (RT-LAMP), gel electrophoresis, and colorimetric detection of the MTF1 gene were performed, the limit of detection (LOD) was confirmed at 10 fg/μL, and the overall processes were completed in 1 h. The detection of an OA biomarker in the form of POCT was reported for the first time in this study. The introduced method is expected to serve as a POCT platform directly applicable by clinicians for easy and rapid identification of OA.
Topics: Humans; Point-of-Care Testing; Molecular Diagnostic Techniques; Limit of Detection; Nucleic Acid Amplification Techniques; Osteoarthritis; Biomarkers; Phenolphthaleins; Sensitivity and Specificity
PubMed: 37232895
DOI: 10.3390/bios13050535 -
MethodsX 2023Natural, inland alkaline soda waters form a particular type of saline waters, characterized by a permanent alkaline chemical property. In many cases only the total...
Natural, inland alkaline soda waters form a particular type of saline waters, characterized by a permanent alkaline chemical property. In many cases only the total alkalinity by methyl-orange titration is reported, without phenolphthalein titration. Therefore, a reliable estimation of carbonates from total alkalinity is essential for a precise scientific chemical classification. The concentration of bicarbonate [HCO ] can be reliably estimated in waters using the Advanced Speciation Method (ASM) if methyl-orange total alkalinity titration and pH data are available, while the concentration of carbonate [CO ] is not reliably estimated by the ASM when interfering factors with acid/base properties (e.g., phosphate, silicate, ammonia, etc.) are present in significant concentrations in natural waters. Therefore, here I present and prove an experimental polynomial function for carbonate estimation with the following equation based on the concentration of bicarbonate: [CO ] = -2.878E-7 ± 5.438E-8 × [HCO ] + 0.069±0.003 × [HCO ] This Boros's carbonate estimation method can contribute to a more efficient evaluation of field water samples with several analytical difficulties.•Bicarbonate can be reliably estimated using the Advanced Speciation Method (ASM).•Estimation of the carbonate concentration using ASM in the presence of interfering acid/base factors in alkaline waters.•Experimental polynomial function for reliable carbonate estimation in alkaline soda waters.
PubMed: 37095870
DOI: 10.1016/j.mex.2023.102175 -
F1000Research 2023Dental caries is a major non-communicable disease of public health concern caused due to freely available dietary sugars. We aimed to compare the sugar content and...
Dental caries is a major non-communicable disease of public health concern caused due to freely available dietary sugars. We aimed to compare the sugar content and erosive potential with duration of use and drug classes of orodispersible tablets (ODTs). We conducted an evaluation of the total sugar content (TSC), Potential of Hydrogen (pH), solubility, and Titratable Acidity (TA) of commonly prescribed 62 ODTs. TA was measured by titrating the samples with known amount of. 0.1N sodium hydroxide (NaOH) with phenolphthalein indicator and pH was determined by digital pH meter. TSC was evaluated by phenol sulphuric acid. Solubility was assessed by filtration. Out of the 62 ODTs, majority were Antimicrobials (n=30). One-quarter of the ODTs (26%) had a mean pH below ≤5.5. No significant difference was seen in the mean pH with respect to different drug classes (p=0.082) and duration of use of ODTs. A significant difference was seen in the mean percentage solubility with respect to drug classes (p<0.001). Antimicrobials had the least percentage of solubility as compared to other drug classes. Antiemetics and proton pump inhibitors (24.33 ± 17.34) had significantly higher mean percentage sugar content than Antimicrobials (23.25 ± 17.16). No significant difference was seen in the mean TSC with respect to various drug classes (p=0.718) and between the duration of use of drugs (P=0.568) respectively. No significant difference was seen in the mean percentage TA with respect to drug class (p=0.123) and duration of use of drugs (p=0.424). Overall, we can conclude that one in four ODT formulations had a pH below 5.5 (critical pH). Only one ODT formulation did not have a sugar content. No difference was seen in the mean pH, sugar content, and TA with respect to duration of use of drugs and drug classes.
Topics: Humans; Sugars; Dental Caries; Tablets; Solubility
PubMed: 37089132
DOI: 10.12688/f1000research.130786.2 -
Molecular Pharmaceutics Apr 2023Despite the recent success of amorphous solid dispersions (ASDs) at enabling the delivery of poorly soluble small molecule drugs, ASD-based dosage forms are limited by...
Despite the recent success of amorphous solid dispersions (ASDs) at enabling the delivery of poorly soluble small molecule drugs, ASD-based dosage forms are limited by low drug loading. This is partially due to a sharp decline in drug release from the ASD at drug loadings surpassing the 'limit of congruency' (LoC). In some cases, the LoC is as low as 5% drug loading, significantly increasing the risk of pill burden. Despite efforts to understand the mechanism responsible for the LoC, a clear picture of the molecular processes occurring at the ASD/solution interface remains elusive. In this study, the ASD/solution interface was studied for two model compounds formulated as ASDs with copovidone. The evolution of a gel layer and its phase behavior was captured with fluorescence confocal microscopy, where fluorescent probes were added to label the hydrophobic and hydrophilic phases. Phase separation was detected in the gel layer for most of the ASDs. The morphology of the hydrophobic phase was found to correlate with the release behavior, where a discrete phase resulted in good release and a continuous phase formed a barrier leading to poor release. The continuous phase formed at a lower drug loading for the system with stronger drug-polymer interactions. This was due to incorporation of the polymer into the hydrophobic phase. The study highlights the complex molecular and phase behavior at the ASD/solution interface of copovidone-based ASDs and provides a thermodynamic argument for qualitatively predicting the release behavior based on drug-polymer interactions.
Topics: Solubility; Drug Liberation; Vinyl Compounds; Pharmaceutical Preparations; Polymers; Drug Compounding
PubMed: 36926898
DOI: 10.1021/acs.molpharmaceut.3c00020 -
Carbohydrate Polymers Jan 2023Nowadays, supramolecular hydrogels have gained special importance and development of versatile approaches for their preparation as well as their new facile...
Nowadays, supramolecular hydrogels have gained special importance and development of versatile approaches for their preparation as well as their new facile characterization strategies has elicited tremendous scientific interest. Herein, we demonstrate that modified cellulose nanowhisker with gallic acid pendant groups (CNW-GA) could effectively bind with CNW grafted with β-Cyclodextrin (CNW-g-β-CD) through HG interaction to form fully biocompatible and low-cost supramolecular hydrogel. Also, we reported an easy and efficient colorimetric characterization method for confirming HG complexation using naked eye. The possibility of this characterization strategy evaluated both experimentally and theoretically using DFT method. Also, phenolphthalein (PP) was used for visual detection of HG complexation. Interestingly, PP undergoes a rearrangement in its structure in presence of CNW-g-β-CD because of HG complexation that turns the purple molecule into a colorless compound in alkaline condition. Addition of CNW-GA to the resulting colorless solution turned the color to purple again which easily confirmed HG formation.
PubMed: 36876823
DOI: 10.1016/j.carbpol.2022.120222 -
Journal of Safety Research Feb 2023Air carriers, but not general aviation, have long employed in-flight data to identify risks/implement corrective measures for improved safety. Herein, using in-flight...
BACKGROUND
Air carriers, but not general aviation, have long employed in-flight data to identify risks/implement corrective measures for improved safety. Herein, using in-flight data, aircraft (in non-instrument-rated private pilots (PPLs) ownership) operations in two potentially hazardous environments (mountains, degraded visibility) were researched for safety practice deficiencies. Four questions were posed, the first two related to mountainous terrain operations: were aircraft (a) flown with hazardous ridge-level winds, (b) within gliding distance of level terrain? Regarding degraded visibility, did aviators (c) depart with low cloud ceilings (≤3,000 ft.), (d) fly at night away from urban lighting?
METHODS
The study cohort comprised: (a) single engine aircraft in sole PPL proprietorship (b) registered in Automatic Dependent Surveillance-Broadcast (ADS-B-Out) equipage-required locations prone to low cloud ceilings in three mountainous states. ADS-B-Out data for cross-country flights (>200 nm) were collected.
RESULTS
250 flights (50 airplanes) were tracked (spring/summer 2021). For aircraft transiting areas subject to mountain winds influences, 65% completed one/multiple flights with potentially hazardous ridge-level winds. Two thirds of airplanes traversing mountainous topography would have, for at least one flight, been unable to glide to level terrain with a powerplant failure. Encouragingly, flight departures for 82% of the aircraft were with >3,000 ft. cloud ceilings. Likewise, flights for >86% of the study cohort were undertaken during daylight. Employing a risk scale, operations for 68% of the study cohort did not exceed low-risk (i.e., ≤1 unsafe practice) and high-risk flight(s) (three concurrent unsafe practices) were rare (4% of airplanes). In log-linear analysis, no interactions were evident between the four unsafe practices (p = 0.602).
DISCUSSION
Hazardous winds and inadequate engine failure planning were identified as safety deficiencies for general aviation mountain operations.
PRACTICAL APPLICATION
This study advocates for the expanded use of ADS-B-Out in-flight data to inform safety deficiencies/implement corrective measures toward improving general aviation safety.
Topics: Aviation; Aircraft; Dioctyl Sulfosuccinic Acid; Lighting; Phenolphthalein
PubMed: 36868678
DOI: 10.1016/j.jsr.2022.10.011 -
Clinics in Orthopedic Surgery Feb 2023Corrective osteotomy is an effective surgery for correcting posture in patients with ankylosing spondylitis (AS). Despite satisfactory correction, some patients...
BACKGROUND
Corrective osteotomy is an effective surgery for correcting posture in patients with ankylosing spondylitis (AS). Despite satisfactory correction, some patients experience re-stooping during follow-up. However, there have been no studies on re-stooping in AS. We aimed to analyze the factors that affect re-stooping.
METHODS
Fifty patients (50 cases) who underwent thoracolumbar corrective osteotomy for AS from March 2006 to April 2018 were analyzed. We defined re-stooping as global kyphosis that recurs after corrective osteotomy. The patients were divided into two groups based on the ratio of correction loss: non-re-stooping group (N group) and re-stooping group (R group). We analyzed the demographic data and radiological parameters, such as modified Stoke Ankylosing Spondylitis Spine Score (mSASSS), sagittal vertical axis, and various angles. We also investigated the factors affecting re-stooping by analyzing the correlation between the ratio of correction loss and various factors.
RESULTS
A significant difference was seen in the change in the mSASSS from before surgery to the last follow-up between the N group (2.87 ± 3.08) and the R group (9.20 ± 5.44). In multivariate analysis, only the change in the mSASSS from before surgery to the last follow-up was significantly correlated with the ratio of correction loss.
CONCLUSIONS
Thoracolumbar corrective osteotomy seems to provide high satisfaction among patients with AS but can lead to re-stooping during follow-up. The change in mSASSS was related with re-stooping in the current study. We recommend active rehabilitative exercises and appropriate medication depending on the patient's condition, which may help delay the postoperative progression of AS.
Topics: Humans; Spondylitis, Ankylosing; Treatment Outcome; Spine; Kyphosis; Osteotomy; Phenolphthalein; Lumbar Vertebrae; Thoracic Vertebrae; Retrospective Studies
PubMed: 36778985
DOI: 10.4055/cios22075 -
Journal of the Mechanical Behavior of... Apr 2023Potential estrogenic effects and changes in fertility are some of the health problems associated with bisphenol A (BPA) derivatives used to produce some polymers,...
UNLABELLED
Potential estrogenic effects and changes in fertility are some of the health problems associated with bisphenol A (BPA) derivatives used to produce some polymers, including dental materials that contain Bis-GMA. Those issues drove this study proposing the synthesis of methacrylate resveratrol and phenolphthalein monomers that, combined with diluent monomers, generate copolymers. Their key characteristics were determined and analyzed on the chemical structure-property perspective considering monomer planarity and flexibility based on molecular dynamic simulations.
METHODS
Methacrylate resveratrol ((E)-5-(4-(methacryloyloxy)styryl)-1,3-phenylenebis(2-methylacrylate)), EMPM) and methacrylate phenolphthalein ((3-oxo-1,3-dihydroisobenzofuran-1,1-diyl)bis(4,1-phenylene)bis(2-methylacrylate)), DIFPM) were synthesized through the reaction of precursors with methacryloyl chloride. After monomers purification and spectroscopic characterization (FTIR and NMR), the following copolymers were produced: DIFPM/TEGDMA and Bis-GMA/TEGDMA, EMPM/HEMA and Bis-GMA/HEMA. Microhardness, degree of conversion, water sorption and contact angle data were statistically analyzed through one-way ANOVA and Tukey's test (p ≤ 0.05).
RESULTS
The DIFPM molecular structure's reduced flexibility proved to be an important factor to inhibit TEGDMA cyclization. In turn, the EMPM molecule's high planarity modified the spatial organization of the HEMA copolymer, altering the water diffusion and, therefore, the water sorption when compared to Bis-GMA copolymers.
CONCLUSION
The scientific findings contribute to better understand the effect of monomer chemical structures, molecular geometry, and planarity on some physicochemical properties of copolymers. Knowledge that can contribute to the design of new monomers to replace Bis-GMA.
Topics: Bisphenol A-Glycidyl Methacrylate; Resveratrol; Phenolphthalein; Methacrylates; Polymethacrylic Acids; Polyethylene Glycols; Polymers; Water; Composite Resins; Materials Testing; Dental Materials
PubMed: 36774763
DOI: 10.1016/j.jmbbm.2023.105701