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International Journal of Systematic and... Jun 2024Strain I65 (=KACC 22647=JCM 35315), a novel Gram-stain-negative, strictly aerobic, non-motile, non-spore-forming, rod-shaped, and orange-pigmented bacterium was isolated...
Strain I65 (=KACC 22647=JCM 35315), a novel Gram-stain-negative, strictly aerobic, non-motile, non-spore-forming, rod-shaped, and orange-pigmented bacterium was isolated from influent water of a wastewater treatment system after treatment with several antibiotics, such as meropenem, gentamicin, and macrolide. The newly identified bacterial strain I65 exhibits significant multi-drug and heavy metal resistance characteristics. Strain I65 was grown in Reasoner's 2A medium [0 %-2 % (w/v) NaCl (optimum, 0 %), pH 5.0-10.0 (optimum, pH 7.0), and 20-45°C (optimum, 30 °C)]. Phylogenetic analysis based on 16S rRNA gene sequencing confirmed that strain I65 was closely related to CCBAU 05354 (99.56 % sequence similarity), THG-DN5.5 (97.51 %), and GR10-1 (97.09 %). Further analysis of the whole-genome sequence confirmed that the digital DNA-DNA hybridization, average nucleotide identity, and average amino acid identity values between strain I65 and CCBAU 05354 were 23.4, 80.7, and 85.0 %, respectively, suggesting that strain I65 is distinct from . The genome size of strain I65 was 6.1 Mbp, as assessed using the Oxford Nanopore platform, and its genomic DNA G+C content was 43.0 mol%. The major fatty acids of strain I65 were iso-C and iso-C G, and the major respiratory quinone was MK-7. Moreover, the major polar lipid of strain I65 was phosphatidylethanolamine. Based on genotypic, chemotaxonomic, and phenotype data, strain I65 represents a novel species belonging to the genus , for which the name sp. nov. is proposed. The type strain is I65 (=KACC 22647=JCM 35315).
Topics: Wastewater; RNA, Ribosomal, 16S; Phylogeny; DNA, Bacterial; Fatty Acids; Bacterial Typing Techniques; Base Composition; Sequence Analysis, DNA; Nucleic Acid Hybridization; Flavobacteriaceae; Anti-Bacterial Agents; Vitamin K 2; Phospholipids; Water Microbiology; Whole Genome Sequencing
PubMed: 38885037
DOI: 10.1099/ijsem.0.006425 -
International Journal of Systematic and... Jun 2024A Gram-stain-negative, orange-yellow, rod-shaped bacterium, designated strain SCSIO 19198, was isolated from sediment of the Haima cold seep in the South China Sea, PR...
A Gram-stain-negative, orange-yellow, rod-shaped bacterium, designated strain SCSIO 19198, was isolated from sediment of the Haima cold seep in the South China Sea, PR China. The strain was aerobic and non-motile. Growth of strain SCSIO 19198 occurred at pH 7-9 (optimum, pH 7), 15-37 °C (optimum, 25-32 °C) and with 3-8 % (w/v) NaCl (optimum, 3-6 % NaCl). Phylogenetic analyses based on 16S rRNA sequences revealed that strain SCSIO 19198 belonged to the genus , having the highest similarity to HD-3 (98.35 %), followed by KYW589 (95.17 %) and GYP-24 (94.89 %). The DNA G+C content was 35.92 mol%. The average nucleotide identity value between the genome of strain SCSIO 19198 and that of HD-3 was 88.49 %. The digital DNA-DNA hybridization value between strain SCSIO 19198 and HD-3 was 36 %. The major fatty acids (>10 %) of strain SCSIO 19198 were iso-C, iso-C G, summed feature 3 (C 6/C ω7) and anteiso-C. MK-6 was the only detected respiratory quinone. The polar lipids of strain SCSIO 19198 included phosphatidylethanolamine, two aminolipids, glycolipid and two unidentified lipids. The phenotypic, phylogenetic, chemotaxonomic and genomic data clearly suggest that strain SCSIO 19198 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is SCSIO 19198 (=MCCC 1K08674=KCTC 102078).
Topics: RNA, Ribosomal, 16S; China; Phylogeny; Fatty Acids; Base Composition; DNA, Bacterial; Bacterial Typing Techniques; Nucleic Acid Hybridization; Geologic Sediments; Sequence Analysis, DNA; Seawater; Vitamin K 2; Molecular Sequence Data
PubMed: 38885036
DOI: 10.1099/ijsem.0.006428 -
International Journal of Systematic and... Jun 2024A novel Gram-stain-negative strain, designated JM10B15, was isolated from pond water for collected from Jiangmen City, Guangdong province, south PR China. Cells of the...
A novel Gram-stain-negative strain, designated JM10B15, was isolated from pond water for collected from Jiangmen City, Guangdong province, south PR China. Cells of the strain were aerobic, rod-shaped, and motile by lateral flagella. JM10B15 could grow at 15-40 °C, pH 6.0-9.5, and in 0-3.0 % NaCl, with optimal growth at 25-35 °C, pH 7.5-8.5, and in 0 % NaCl, respectively. Furthermore, this strain grew well on Reasoner's 2A agar but not on nutrient broth agar or Luria-Bertani agar. JM10B15 was a denitrifying bacterium capable of removing nitrites and nitrates, and three key functional genes, , , and , were identified in its genome. The results of phylogenetic analyses based on the 16S rRNA gene and genome sequences indicated that JM10B15 belonged to the genus . JM10B15 showed the highest 16S rRNA sequence similarity to YJ-T1-11 (98.8 %), followed by IFAM 1031 (98.6 %) and HB-1 (98.1 %). The average nucleotide identity and digital DNA-DNA hybridization values between JM10B15 and the other type strains of genus were 78.1-82.1 % and 18.4-22.1 %, respectively. The major fatty acids of strain JM10B15 were summed feature 8 (C 6 and/or C 7) and C 7 11-methyl. In addition, the major respiratory quinone of this novel strain was Q-10, and the predominant polar lipids were phosphatidylglycerol, phosphatidylethanolamine, four unidentified phospholipids, three unidentified lipids, and an unidentified aminophospholipid. Results of analyses of the phylogenetic, genomic, physiological, and biochemical characteristics indicated that JM10B15 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is JM10B15 (=GDMCC 1.4148=KCTC 8140).
Topics: Phylogeny; RNA, Ribosomal, 16S; Fatty Acids; Ponds; DNA, Bacterial; Denitrification; China; Bacterial Typing Techniques; Base Composition; Animals; Nucleic Acid Hybridization; Sequence Analysis, DNA; Penaeidae; Phospholipids; Water Microbiology; Nitrates; Ubiquinone; Nitrites
PubMed: 38885035
DOI: 10.1099/ijsem.0.006430 -
Heliyon Jun 2024Bisphenol A (BPA) is a common environmental endocrine disruptor that negatively impairs male reproductive ability. This study aimed to explore the alterations in serum...
OBJECTIVE
Bisphenol A (BPA) is a common environmental endocrine disruptor that negatively impairs male reproductive ability. This study aimed to explore the alterations in serum metabolomics that occur following BPA exposure and the mechanism via which BPA induces the death of testicular cells in a male mouse model.
METHODS
The mice were classified into two groups: BPA-exposed and control groups, and samples were collected for metabolomic determination, semen quality analysis, electron microscopy, enzyme-linked immunosorbent assay, quantitative real-time PCR, pathological staining, and Western blot analysis.
RESULTS
BPA exposure caused testicular damage and significantly decreased sperm quality in mice. Combined with non-target metabolomic analysis, this was closely related to ferroptosis induced by abnormal metabolites of arachidonic acid and phosphatidylcholine, and the expression of its related genes, acyl CoA synthetase 4, glutathione peroxidase 4, lysophosphatidylcholine acyltransferase 3, and phosphatidylethanolamine-binding protein 1 were altered.
CONCLUSION
BPA induced ferroptosis, caused testicular damage, and reduced fertility by affecting lipid metabolism in male mice. Inhibiting ferroptosis may potentially function as a therapeutic strategy to mitigate the male reproductive toxicity induced by BPA.
PubMed: 38882385
DOI: 10.1016/j.heliyon.2024.e31667 -
Journal of Ethnopharmacology Jun 2024Liver fibrosis is a generic fibrous scarring event resulting from accumulation of extracellular matrix (ECM) proteins, easily progressing to end-stage liver diseases....
ETHNOPHARMACOLOGICAL RELEVANCE
Liver fibrosis is a generic fibrous scarring event resulting from accumulation of extracellular matrix (ECM) proteins, easily progressing to end-stage liver diseases. Tao-Hong-Si-Wu-Tang (THSWT) is a traditional Chinese medicine formula applied in clinics to treat gynecological and chronic liver diseases. However, the role of THSWT on thioacetamide (TAA)-induced hepatic fibrosis and the specific mechanisms remains unclear.
AIM OF THE STUDY
To investigate the improving effects of THSWT on TAA-insulted hepatic fibrosis and the underlying mechanisms.
MATERIALS AND METHODS
UHPLC-MS/MS was performed to explore the chemical characterization of THSWT. Mice were orally administered with THSWT once daily for 6 weeks along with TAA challenge. Liver function was reflected through serum biomarkers and histopathological staining. RNA sequencing, non-targeted metabolomics and molecular biology experiments were applied to investigate the underlying mechanisms.
RESULTS
THSWT profoundly repaired lipid metabolism dysfunction and blocked collagen accumulation both in TAA-stimulated mice and in hepatocytes. Results of RNA sequencing and non-targeted metabolomics revealed that the anti-fibrotic effects of THSWT mostly relied on lipid metabolism repairment by increasing levels of acetyl-CoA, phosphatidylcholine, phosphatidylethanolamine, lysophosphatidylcholine and lysophosphatidylethanolamine, and decreasing relative abundances of acyl-CoA, total cholesterol, diacylglycerol, triacylglycerol and phosphatidylinositol. Mechanically, long-chain acyl-CoA synthetases 4 (ACSL4) was a key profibrotic target both in human and mice by disrupting lipid oxidation and metabolism in hepatic mitochondria. THSWT effectively blocked ACSL4 and promoted mitophagy to reverse above outcomes, which was verified by mitophagy depletion.
CONCLUSION
THSWT may be a promising therapeutic option for treating hepatic fibrosis and its complications by modulating lipid metabolism and promoting mitophagy in livers.
PubMed: 38878839
DOI: 10.1016/j.jep.2024.118456 -
Food Research International (Ottawa,... Aug 2024Comprehensive lipid and volatile compound analyses were performed with squids collected from four varied geographical locations to discriminate the regional...
Lipidomics profile and volatile compounds of squids (Illex argentinus, Ommastrephes Bartram and Dosidicus gigas) in the Argentine, North Pacific Ocean, Equator and Peru by UPLC-triple TOF-MS and HS-SPME-GC-O-MS.
Comprehensive lipid and volatile compound analyses were performed with squids collected from four varied geographical locations to discriminate the regional characteristics. A total of 1442 lipid molecules and 110 volatiles were detected in the squid muscle samples. There were significant differences in the lipid profiles between Argentine squid (Illex argentinus, AGT), North Pacific Ocean squid (Ommastrephes Bartram, NPO), Equatorial squid (Dosidicus gigas, EQ), and Peruvian squid (Dosidicus gigas, PR) muscle. Phosphatidylcholines (14.64%), triacylglycerols (12.42%), and ceramides (10.97%) were the main lipid components. The contents of polyunsaturated fatty acid in phospholipids and in glycerolipids were 30.35-52.05% and 18.11-25.15%, respectively. The volatiles in squids exhibited significant regional variation; 1-pentanol and 1-octanol, 2-ethyl-1-hexanol and terpinen-4-ol, 2,7-ethyl-1-hexanol, 3-methy-1-butanol and 2-propyl-1-pentanol were identified as characteristic flavor compounds in AGT, NPO, EQ, and PR, respectively. Sphingomyelin, phosphatidylserine, phosphatidylethanolamine, and ceramide were strongly correlated with volatiles in squid muscle. Our study is a reference for the lipid nutritional value and flavor compounds of squids.
Topics: Animals; Decapodiformes; Volatile Organic Compounds; Pacific Ocean; Lipidomics; Gas Chromatography-Mass Spectrometry; Argentina; Peru; Chromatography, High Pressure Liquid; Solid Phase Microextraction; Triglycerides; Lipids; Phospholipids; Muscles
PubMed: 38876608
DOI: 10.1016/j.foodres.2024.114559 -
Food Chemistry Jun 2024The major lipids and antioxidant activities of Asterias rolleston gonad lipids were evaluated systematically. Major lipids of A. Rolleston gonad lipids were...
The major lipids and antioxidant activities of Asterias rolleston gonad lipids were evaluated systematically. Major lipids of A. Rolleston gonad lipids were triacylglycerols (TAGs) and phospholipids (PLs). Total lipids were composed of 15.62% of polyunsaturated fatty acids (PUFAs), and 40.81% of monounsaturated fatty acids (MUFAs). The most abundant PUFA were C20:5n-3 (EPA) (6.28%) and C22:6n-3 (DHA) (5.80%). Predominantly composed of phosphatidylcholine (PC) and phosphatidylethanolamine (PE), polar lipids were rich in PUFAs and could contain up to 34.59% EPA and DHA, and PE and PI (phosphatidylinositol) were also found to be the main carriers of EPA and ARA (arachidonic acid) in polar lipids. The MUFA and PUFA of Sn-2 in TAG are 39.72% and 30.37%, respectively. A total of 64 TAG species were identified, with Eo-P-M, Eo-Eo-M, and M-M-Eo being the main TAGs components. Moreover, A. rollestoni gonad lipids exhibited potent radical scavenging activities and reducing power in a dose-dependent manner.
PubMed: 38870815
DOI: 10.1016/j.foodchem.2024.140005 -
Food Chemistry Jun 2024Lipids are key aroma contributors in meat products. However, the role of different lipids in the presence of aroma compounds in roasted pigeons has not been studied. The...
Lipids are key aroma contributors in meat products. However, the role of different lipids in the presence of aroma compounds in roasted pigeons has not been studied. The formation of aroma compounds and lipids during the circulating non-fried roasting of pigeons was investigated. The results presented that 18 aroma compounds, including 5-methy-2,3-diethylpyrazine, were identified as key aroma compounds. A total of 6324 lipids were classed into 47 categories, such as phosphatidylcholine (PC), phosphatidylethanolamine (PE), and triglyceride (TG). Nine lipids, containing PA(P-20:0/22:4(7Z,10Z,13Z,16Z)) and LPC 16:0-SN1, showed promise as potential biomarkers for discriminating differential pigeons using OPLS-DA. PC (13.76%), TG (13.58%), and their products were major lipids, among which TG 16:0 16:0 18:2, LPC 18:2-SN1, and PC 18:1_18:1 played a crucial role in the presence of aroma compounds. Interestingly, the linoleic acid, an important aroma contributor, was predominantly bonded to the sn-2 position of phospholipid and sn-3 position of neutral lipids.
PubMed: 38870809
DOI: 10.1016/j.foodchem.2024.139960 -
Colloids and Surfaces. B, Biointerfaces May 2024The interaction between nucleotide molecules and lipid molecules plays important roles in cell activities, but the molecular mechanism is very elusive. In the present...
The interaction between nucleotide molecules and lipid molecules plays important roles in cell activities, but the molecular mechanism is very elusive. In the present study, a small but noticeable interaction between the negatively charged phosphatidylethanolamine (PE) and Guanosine monophosphate (GMP) molecules was observed from the PE monolayer at the air/water interface. As shown by the sum frequency generation (SFG) spectra and Pi-A isotherm of the PE monolayer, the interaction between the PE and GMP molecules imposes very small changes to the PE molecules. However, the Brewster angle microscopy (BAM) technique revealed that the assembly conformations of PE molecules are significantly changed by the adsorption of GMP molecules. By comparing the SFG spectra of PE monolayers after the adsorption of GMP, guanosine and guanine, it is also shown that the hydrogen bonding effect plays an important role in the nucleotide-PE interactions. These results provide fundamental insight into the structure changes during the nucleotide-lipid interaction, which may shed light on the molecular mechanism of viral infection, DNA drug delivery, and cell membrane curvature control in the brain or neurons.
PubMed: 38870647
DOI: 10.1016/j.colsurfb.2024.113995 -
International Journal of Systematic and... Jun 2024Two novel strains, designated APW6 and APW11, were isolated from artificial pond water, and one novel strain, designated PFR6, was isolated from a root. These strains...
Two novel strains, designated APW6 and APW11, were isolated from artificial pond water, and one novel strain, designated PFR6, was isolated from a root. These strains were found to be Gram-negative, rod-shaped, motile by means of flagella, and oxidase-positive. Growth conditions of the type strains were as follows: APW6, 15-43 °C (optimum, 28 °C), pH 6.0-12.0 (optimum, pH 7.0), with no salinity; APW11, 4-35 °C (optimum, 25 °C), pH 6.0-11.0 (optimum, pH 9.0), with 0-1 % NaCl (w/v, optimum 0 %); PFR6, 10-38 °C (optimum 28 °C), pH 6.0-12.0 (optimum, pH 7.0), with 0-2 % NaCl (w/v; optimum, 0 %). Strains APW6, APW11, and PFR6 belonged to the genus , having the most 16S rRNA gene sequence similarity to DSM 654 (98.1 %), CHU3 (98.7 %), and CCUG 52769 (98.1 %). The estimated genome sizes of APW6, APW11, and PFR6 were 50 50 473, 56 70 008, and 52 16 869 bp, respectively and the G+C contents were 69.5, 66, and 68.5 mol%. The digital DNA-DNA hybridization, average amino acid identity, and average nucleotide identity values among the novel strains and related taxa were all lower than 22.4, 74.7, and 78.9 %, respectively. The predominant cellular fatty acids (>10 %) of all strains were summed feature 3 (comprising C 6 and/or C 7) and C. PFR6 also had summed feature 8 (comprising C 7 and/or C 6) as a major fatty acid. The polar lipid profile of all strains contained phosphatidylethanolamine, phosphoaminoglycolipid, and phosphoglycolipid. The distinct phylogenetic, physiological, and chemotaxonomic features reported in this study indicate that strains APW6, APW11, and PFR6 represent novel species within the genus , for which the names sp. nov., with the type strain APW6 (=KACC 22877=TBRC 16606), sp. nov., with the type strain APW11 (=KACC 22878=TBRC 16607), and sp. nov (=KACC 23257=TBRC 17653) are respectively proposed.
Topics: Phylogeny; Base Composition; Ponds; RNA, Ribosomal, 16S; Fatty Acids; DNA, Bacterial; Bacterial Typing Techniques; Sequence Analysis, DNA; Plant Roots; Rhodobacteraceae; Nucleic Acid Hybridization; Water Microbiology
PubMed: 38869492
DOI: 10.1099/ijsem.0.006426