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Journal of Anaesthesiology, Clinical... 2022Blood loss in neurosurgical procedures can be rapid and tremendous leading to consequential hemodynamic instability. HemoCue is a portable photometer used for the...
BACKGROUND AND AIMS
Blood loss in neurosurgical procedures can be rapid and tremendous leading to consequential hemodynamic instability. HemoCue is a portable photometer used for the measurement of blood hemoglobin concentration. Using this point of care device, we contemplated this study to assess the reliability of HemoCue for suction hemoglobin determination and calculation of surgical blood loss by comparing with the gold standard laboratory Coulter Counter method in patients undergoing neurosurgical procedures.
MATERIAL AND METHODS
This prospective observational study was conducted in 233 patients undergoing elective neurosurgical procedures in the age group of 18 to 60 years and having preoperative hemoglobin (Hb) values above 9 g/dL. Values of preoperative hemoglobin, suction hemoglobin, and volume in the suction container were used to obtain the estimated blood loss. The normality of the data was tested using the Kolmogorov Smirnov test. Bland Altman analysis was used to test the reliability of HemoCue in estimating hemoglobin and blood loss with reference to the gold standard laboratory Coulter Counter automated hematology analyzer.
RESULTS
Median blood loss calculated in our study using HemoCue was 554.65 mL with an interquartile range of 336.81 mL to 982.39 mL. Laboratory counter method estimated median blood loss was 533.37 mL with an interquartile range of 335.21 mL to 994.73 mL. The majority of the data obtained and analyzed using the Bland and Altman analysis method were within a 95% confidence interval.
CONCLUSION
The HemoCue method is a reliable method and henceforth can be used to estimate blood loss in suction fluid in patients undergoing neurosurgical procedures.
PubMed: 36778832
DOI: 10.4103/joacp.JOACP_638_20 -
The Science of the Total Environment May 2023Dust particles originating from arid desert regions can be transported over long distances, presenting severe risks to climate, environment, social economics, and human...
Dust particles originating from arid desert regions can be transported over long distances, presenting severe risks to climate, environment, social economics, and human health at the source and downwind regions. However, there has been a dearth of continuous diurnal observations of vertically resolved mass concentration and optical properties of dust aerosols, which hinders our understanding of aerosol mixing, stratification, aerosol-cloud interactions, and their impacts on the environment. To fill the gap of the insufficient observations, to the best of our knowledge, this work presents the first high-spectral-resolution lidar (HSRL) observation providing days of continuous profiles of the mass concentration, along with particle linear depolarization ratio (PLDR), backscattering coefficient, extinction coefficient and lidar ratio (LR), simultaneously. We present the results of two strong dust events observed by HSRL over Beijing in 2021. The maximum particle mass concentrations reached (1.52 ± 3.5) x10 μg/m and (19.48 ± 0.36) x10 μg/m for the two dust events, respectively. The retrieved particle mass concentrations and aerosol optical depth (AOD) agree well with the observation from the surface PM concentrations and sun photometer with correlation coefficients of 0.90 and 0.95, respectively. The intensive properties of PLDR and LR of the dust aerosols are 0.31 ± 0.02 and 39 ± 7 sr at 532 nm, respectively, which are generally close to those obtained from observations in the downwind areas. Moreover, inspired by the observations from HSRL, a universal analytical relationship is discovered to evaluate the proportion of dust aerosol backscattering, extinction, AOD, and mass concentration using PLDR. The universal analytical relationship reveals that PLDR can directly quantify dust aerosol contribution, which is expected to further expand the application of polarization technology in dust detection. These valuable observations and findings further our understanding of the contribution of dust aerosol to the environment and help supplement dust aerosol databases.
PubMed: 36758704
DOI: 10.1016/j.scitotenv.2023.162091 -
Annales de Biologie Clinique Nov 2022In pediatrics, accurate measurement of total serum bilirubin (TSB) is of major importance for reliable diagnosis and appropriate management of neonatal jaundice....
In pediatrics, accurate measurement of total serum bilirubin (TSB) is of major importance for reliable diagnosis and appropriate management of neonatal jaundice. However, several studies evidenced poor comparability of results obtained with the different available methods either in central lab or in POCT, on serum, capillary blood or transcutaneous. This situation is partly due to the lack of Reference Materials, especially for high bilirubin concentrations but also on poor communication between central lab and neonatology unit. To progress on these issues, we have compiled some data from CNRHP to propose guidelines for choice, use and management of POCT devices and to help clinical laboratories to achieve a better answer to clinical needs with specific local constraints. The results from several CNRHP studies are presented: traceability to International System of Units, inter-laboratories comparability, POCT vs central labs comparisons with POCT CO-oximeter or photometer, integration of transcutaneous bilirubinometer. We propose, based on an analysis of devices advantages and issues, guidelines to help labs either to improve neonates monitoring in their local context; we distinguished the choices inside laboratory for a better harmonization of results compared to published thresholds and outside lab contexts, to organize a coordinated chain with POCT devices, with capillary and/or transcutaneous approaches.
Topics: Infant, Newborn; Humans; Child; Kernicterus; Neonatal Screening; Bilirubin; Hyperbilirubinemia, Neonatal; Jaundice, Neonatal
PubMed: 36696559
DOI: 10.1684/abc.2022.1774 -
Diagnostics (Basel, Switzerland) Jan 2023This article systematizes available data from the literature on biliary gastritis (BG) in order to increase the awareness of specialists about the latest possibilities... (Review)
Review
This article systematizes available data from the literature on biliary gastritis (BG) in order to increase the awareness of specialists about the latest possibilities for diagnosing the disease. BG occurs as a result of pathological duodenogastric reflux. In patients with a preserved duodenogastric junction, the dominant factor is represented by motor disorders of the upper digestive tract (primary biliary gastritis), while in patients recovering from surgical interventions it is represented by structural changes (secondary biliary gastritis). Progressive BG can lead to atrophy of the gastric mucosa, intestinal metaplasia, epithelial dysplasia, and eventually to gastric cancer. Diagnostic methods for BG are carried out to identify risk factors, exclude alarm symptoms and identify persistent motor disorders and pathological reflux (24 h pH-impedancemetry, hepatobiliary scintigraphy, 24 h monitoring of bilirubin content in the reflux using a Bilitec 2000 photometer), as well as to diagnose gastritis itself (esophagogastroduodenoscopy, morphological gastrobiopsy examination). The diagnosis of BG should be based on a multidisciplinary approach that combines a thorough analysis of a patient's complaints, an anamnesis of the disease, and the results of endoscopic and histological research methods.
PubMed: 36672996
DOI: 10.3390/diagnostics13020186 -
Saudi Journal of Ophthalmology :... 2022Aqueous flare and cells are inflammatory parameters of anterior chamber inflammation resulting from disruption of the blood-ocular barrier. The ocular inflammation...
Aqueous flare and cells are inflammatory parameters of anterior chamber inflammation resulting from disruption of the blood-ocular barrier. The ocular inflammation related to anterior chamber cells and flare is assessed by conventional clinical grading systems through using the slit-lamp examination. However, a more quantitative and objective assessment is needed for more precise and reproducible inflammatory assessment in uveitis. Laser flare photometer (LFP) was introduced as a noninvasive, objective, and quantitative evaluation of aqueous flare intensity and number of cells in the anterior chamber with good accuracy and repeatability. The success of LFP allowed clinicians to further evaluate the pathophysiology of intra-ocular inflammation and to incorporate LFP measurements to their routine clinical practice for diagnosis, management, and treatment of uveitis cases. In this review, we will discuss the importance of clinical utilization of LFP and the correlation between LFP and clinical grading systems along with some technical aspects. Furthermore, we will give a literature summary on the current applications of LFP in clinical practice of cases which present with various types of uveitis and diverse ocular conditions with or without inflammation.
PubMed: 36618569
DOI: 10.4103/sjopt.sjopt_119_22 -
Talanta Apr 2023Uric acid (UA) is the final product of purine metabolism. A high concentration of UA in body fluid may lead to kidney stones, gout, and some cardiovascular diseases....
Uric acid (UA) is the final product of purine metabolism. A high concentration of UA in body fluid may lead to kidney stones, gout, and some cardiovascular diseases. Therefore, the non-invasive daily monitoring of UA is of great significance for both hyperuricemia patients and fit people. However, most of the current detection methods for UA are enzyme-dependent which limits the application scenarios and lacks portable instruments for on-site detection, including optics and electrochemistry. In this work, an enzyme-free and wide-range colorimetric sensor for UA and HO detection was developed based on a mercaptosuccinic acid (MSA)-modified Cu nanoparticles (CuNPs). Under the action of UA or HO, with the cleavage of MSAs on the CuNPs surface, small Cu particles are further aggregated into larger particles with a lightning violet color. With the employment of the multi-channel handheld automatic photometer (MHAP), the concentration of UA and HO can be determined on-site according to the absorbance measurement by the photodiodes. The linear range of UA was 5 μM-4.5 mM with the limit of detection (LOD) of 3.7 μM, while the linear range of HO was 5 mM-500 mM and 5 μM-5 mM with the LOD of 4.3 μM. This approach has been applied to the detection of UA in human urine, providing more possibilities for non-invasive home health monitoring, community medical diagnosis, and broader prospects of on-site disease detection.
Topics: Humans; Uric Acid; Hydrogen Peroxide; Copper; Colorimetry; Nanoparticles
PubMed: 36565527
DOI: 10.1016/j.talanta.2022.124196 -
Science Bulletin Oct 2022The Hunga Tonga-Hunga Ha'apai eruption on January 15, 2022 was one of the most explosive volcanic eruptions of the 21st century and has attracted global attention. Here...
The Hunga Tonga-Hunga Ha'apai eruption on January 15, 2022 was one of the most explosive volcanic eruptions of the 21st century and has attracted global attention. Here we show that large numbers of the volcanic aerosols from the eruption broke through the tropopause into the lower stratosphere, forming an ash plume with an overshooting top at 25-30 km altitude. In the four days following the eruption, the ash plume moved rapidly westward for nearly 10,000 km under stable stratospheric conditions characterized by strong tropical easterlies, weak meridional winds and weak vertical motion. The intrusion of the ash plume into the stratosphere resulted in a marked increase in atmospheric aerosol loading across northern Australia, with the aerosol optical depth (AOD) observed by satellites and sun-photometers peaking at 1.5 off the coast of northeastern Australia; these effects lasted for nearly three days. The ash plume was characterized by fine-mode particles clustered at a radius of about 0.26 µm, with an observed peak volume of 0.25 µm µm. The impact of the ash plume associated with the Hunga Tonga eruption on the stratospheric AOD and radiative balance in the tropical southern hemisphere is remarkable, with an observed volcanic-induced perturbation of the regional stratospheric AOD of up to 0.6. This perturbation largely explains an instantaneous bottom (top) of the atmosphere radiative forcing of -105.0 (-65.0) W m on a regional scale.
PubMed: 36546211
DOI: 10.1016/j.scib.2022.08.018 -
Journal of Affective Disorders Feb 2023Circadian activity rhythm disruption is a core feature in bipolar disorder. We investigated whether light exposure in daily life is associated with circadian activity...
BACKGROUND
Circadian activity rhythm disruption is a core feature in bipolar disorder. We investigated whether light exposure in daily life is associated with circadian activity rhythms in patients with bipolar disorder.
METHODS
In a cross-sectional study, we enrolled 194 outpatients with bipolar disorder who were participants of the Association between Pathology of Bipolar Disorder and Light Exposure in Daily Life (APPLE) cohort study. The participants' physical activity and daytime illuminance were measured using an actigraph over 7 consecutive days. Nighttime illuminance in the bedroom was measured using a portable photometer. Circadian activity rhythm parameters were calculated using cosinor analysis and a nonparametric circadian rhythm analysis.
RESULTS
The median daytime illuminance and nighttime illuminance were 224.5 lx (interquartile range, 154.5-307.5 lx) and 2.3 lx (0.3-9.4 lx), respectively. Multivariable linear regression analysis, adjusted for potential confounding factors, showed that higher daytime illuminance was significantly associated with higher amplitude and most active continuous 10-hour period, advanced acrophase, higher interdaily stability, and lower intradaily variability. Higher nighttime illuminance was significantly associated with lower relative amplitude, delayed onset of the least active continuous 5-hour period, and higher intradaily variability.
LIMITATIONS
As this was a cross-sectional study, the results do not necessarily imply that light exposure alters circadian activity rhythms.
CONCLUSIONS
Daytime light exposure was associated with a positive effect and nighttime light exposure with a negative effect on circadian activity rhythms in bipolar disorder.
Topics: Humans; Cross-Sectional Studies; Bipolar Disorder; Cohort Studies; Actigraphy; Circadian Rhythm
PubMed: 36538951
DOI: 10.1016/j.jad.2022.12.034 -
Toxicology Reports 2022The recent expansion of industries in Addis Ababa is causing additional environmental pollution through wastewater discharges; this is becoming a critical concern. Addis...
The recent expansion of industries in Addis Ababa is causing additional environmental pollution through wastewater discharges; this is becoming a critical concern. Addis Ababa is located in the upper Awash River basin, and is the main source of industrial pollutants to the river. In this study, physicochemical parameters, nutrients and heavy metal content of wastewaters released from 16 factories, 6 tanneries, 6 beverages and 4 diverse factories, and the Akaki-Kality central wastewater treatment plant in Addis Ababa, were sampled to assess the level of pollutants. Heavy metals were determined using inductively coupled plasma optical emission spectroscopy (ICP-OES). Analysis of nutrients were conducted using Palintest Photometer. Physicochemical characteristics were measured either in situ using a portable micro meter or in the laboratory. Among the measured physicochemical properties, critical issues were observed with electrical conductivity, total dissolved solids and total hardness. Effluents from all of the tanneries, and a number of other factories, were found at levels higher than the maximum limits of various guideline standards. In addition, samples from two of the tanneries (T1 and T5), two beverage factories (B3 and B6) and the central wastewater treatment plant showed elevated concentrations of PO , which violated the limit (10 mg/l) set by Environmental Protection Agency of Ethiopia (ETHEPA). The two tanneries (T1 and T5) also contained higher SO than the guideline limit of 1000 mg/l. On the other hand, only one factory, one brewery (B3), exhibited NO above the standard limit of 20 mg/l. Whereas NH, NH , Cl, S and NO were within the limits in all of the samples. Severe pollution was found in wastewaters from tanneries, where half of them (T1, T5 and T6) contained Cr beyond the maximum limit of 2000 µg/l. Furthermore, a third of the tanneries (T1 and T5) and a beverage factory (B5) contained Fe, Mn, Zn and Cu, higher than the ETHEPA limits of 10000, 5000, 5000 and 2000 µg/l, respectively. Waste disposal from factories without proper treatment can cause great harm to the local people and the environment. Hence, the results of this study call for regulatory bodies to pay close attention to factories, particularly tanneries, in Addis Ababa in implementing adequate treatments of their wastewater discharges.
PubMed: 36518430
DOI: 10.1016/j.toxrep.2022.06.002 -
Toxicology Reports 2022The rapid development associated with Next Generation Tobacco Products (NGTP) has necessitated the development of high throughput methodologies to test their genotoxic...
Characterization of smoke and aerosol deliveries from combustible cigarettes, heated tobacco products and electronic nicotine delivery systems in the Vitrocell® Mammalian 6/48 exposure module.
The rapid development associated with Next Generation Tobacco Products (NGTP) has necessitated the development of high throughput methodologies to test their genotoxic potential in vitro when compared to conventional cigarette smoke (CS). An assessment of two Vitrocell® Mammalian 6/48 exposure modules in three independent experiments was made by comparing results from multiple dosimetric techniques applied to aerosol generated from 3R4F Kentucky Reference cigarettes, commercially available electronically heated tobacco product (eHTP) and Electronic Nicotine Delivery System (ENDS) using the Vitrocell® VC10®. Real-time aerosol particle concentration was assessed by means of light scattering photometers and expressed as area under the curve (∑AUC). Nicotine concentrations were determined analytically by LC/MS. Humectant amount and distribution was assessed for eHTP and ENDS by the quantification of free glycerol in a phosphate buffered saline (PBS) trap, whereas total particulate matter (TPM) was assessed in the 3R4F cigarettes by the fluorescence of the particulate at 485 nm in anhydrous dimethyl sulfoxide (DMSO) trap within the exposure. Dose was adjusted by means of the addition of ambient air to dilute the whole smoke/aerosol in L/min and sampled into the system at a rate of 5 mL/min. Dilution of CS ranged from 8.0 to 0.5 L/min and for the eHTP and ENDS ranged from 4 to 0 L/min (undiluted). Dosimetric analysis of the system showed good concordance within replicates (p-values ranged from p = 0.3762 to p = 0.8926) and showed that the Vitrocell® Mammalian 6/48 is a viable means for genotoxic assessment of aerosol generated from both conventional cigarettes and NGTP. Results demonstrate the need to tailor dosimetry approaches to different aerosols due to variations in the physio-chemical composition, with a multi-dosimetry approach recommended.
PubMed: 36518380
DOI: 10.1016/j.toxrep.2022.11.001