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International Journal of Molecular... Jun 2024Rich biological information in sweat provides great potential for health monitoring and management. However, due to the complexity of sweat, the development of...
Rich biological information in sweat provides great potential for health monitoring and management. However, due to the complexity of sweat, the development of environmentally friendly green electronic products is of great significance to the construction of ecological civilization. This study utilized a simple combination of polystyrene sulfonate sodium (PSS) and filter paper (FP) to prepare cellulose materials coated with conductive polymers, developing an electrochemical sensor based on the modified materials. The mechanical and electrochemical properties of the fabricated PSS/FP membrane were optimized by adjusting the feeding dosage of PSS. The realized PSS/FP composite containing 7% PSS displayed good conductivity (9.1 × 10 S/m), reducing electric resistance by 99.2% compared with the original FP membrane (6.7 × 10 S/m). The stable current of the membrane in simulated sweat under different pH environments is highly correlated with the pH values. Additionally, when the membrane is exposed to simulated sweat with varying ion concentrations, the current signal changes in real time with the concentration variations. The response time averages around 0.3 s.
Topics: Sweat; Cellulose; Hydrogen-Ion Concentration; Electric Conductivity; Polystyrenes; Polymers; Humans; Electrochemical Techniques; Biosensing Techniques
PubMed: 38928100
DOI: 10.3390/ijms25126393 -
Bioengineering (Basel, Switzerland) Jun 2024Biophysical factors play a fundamental role in human embryonic development. Traditional in vitro models of organogenesis focused on the biochemical environment and did... (Review)
Review
Biophysical factors play a fundamental role in human embryonic development. Traditional in vitro models of organogenesis focused on the biochemical environment and did not consider the effects of mechanical forces on developing tissue. While most human tissue has a Young's modulus in the low kilopascal range, the standard cell culture substrate, plasma-treated polystyrene, has a Young's modulus of 3 gigapascals, making it 10,000-100,000 times stiffer than native tissues. Modern in vitro approaches attempt to recapitulate the biophysical niche of native organs and have yielded more clinically relevant models of human tissues. Since Clevers' conception of intestinal organoids in 2009, the field has expanded rapidly, generating stem-cell derived structures, which are transcriptionally similar to fetal tissues, for nearly every organ system in the human body. For this reason, we conjecture that organoids will make their first clinical impact in fetal regenerative medicine as the structures generated ex vivo will better match native fetal tissues. Moreover, autologously sourced transplanted tissues would be able to grow with the developing embryo in a dynamic, fetal environment. As organoid technologies evolve, the resultant tissues will approach the structure and function of adult human organs and may help bridge the gap between preclinical drug candidates and clinically approved therapeutics. In this review, we discuss roles of tissue stiffness, viscoelasticity, and shear forces in organ formation and disease development, suggesting that these physical parameters should be further integrated into organoid models to improve their physiological relevance and therapeutic applicability. It also points to the mechanotransductive Hippo-YAP/TAZ signaling pathway as a key player in the interplay between extracellular matrix stiffness, cellular mechanics, and biochemical pathways. We conclude by highlighting how frontiers in physics can be applied to biology, for example, how quantum entanglement may be applied to better predict spontaneous DNA mutations. In the future, contemporary physical theories may be leveraged to better understand seemingly stochastic events during organogenesis.
PubMed: 38927855
DOI: 10.3390/bioengineering11060619 -
BMC Plant Biology Jun 2024Microplastic (MP) pollution in terrestrial ecosystems is gaining attention, but there is limited research on its effects on leafy vegetables when combined with heavy...
Microplastic (MP) pollution in terrestrial ecosystems is gaining attention, but there is limited research on its effects on leafy vegetables when combined with heavy metals. This study examines the impact of three MP types-polyethylene (PE), polyethylene terephthalate (PET), and polystyrene (PS)-at concentrations of 0.02, 0.05, and 0.1% w/w, along with cadmium (Cd) and biochar (B), on germination, growth, nutrient absorption, and heavy metal uptake in red amaranth (Amaranthus tricolor L.). We found that different MP types and concentrations did not negatively affect germination parameters like germination rate, relative germination rate, germination vigor, relative germination vigor, and germination speed. However, they increased phytotoxicity and decreased stress tolerance compared to an untreated control (CK1). The presence of MPs, particularly the PS type, reduced phosphorus and potassium uptake while enhancing Cd uptake. For example, treatments PSCdB, PSCdB, and PSCdB increased Cd content in A. tricolor seedlings by 158%, 126%, and 44%, respectively, compared to the treatment CdB (CK2). Additionally, MP contamination led to reduced plant height, leaf dry matter content, and fresh and dry weights, indicating adverse effects on plant growth. Moreover, the presence of MPs increased bioconcentration factors and translocation factors for Cd, suggesting that MPs might act as carriers for heavy metal absorption in plants. On the positive side, the addition of biochar improved several root parameters, including root length, volume, surface area, and the number of root tips in the presence of MPs, indicating potential benefits for plant growth. Our study shows that the combination of MPs and Cd reduces plant growth and increases the risk of heavy metal contamination in food crops. Further research is needed to understand how different MP types and concentrations affect various plant species, which will aid in developing targeted mitigation strategies and in exploring the mechanisms through which MPs impact plant growth and heavy metal uptake. Finally, investigating the potential of biochar application in conjunction with other amendments in mitigating these effects could be key to addressing MP and heavy metal contamination in agricultural systems.
Topics: Amaranthus; Cadmium; Charcoal; Microplastics; Soil Pollutants; Germination; Nutrients; Seedlings
PubMed: 38926861
DOI: 10.1186/s12870-024-05312-0 -
Analytical and Bioanalytical Chemistry Jun 2024Flow cytometry plays a pivotal role in biotechnology by providing quantitative measurements for a wide range of applications. Nonetheless, achieving precise particle...
Flow cytometry plays a pivotal role in biotechnology by providing quantitative measurements for a wide range of applications. Nonetheless, achieving precise particle quantification, particularly without relying on counting beads, remains a challenge. In this study, we introduce a novel exhaustive counting method featuring a sample loop-based injection system that delivers a defined sample volume to a detection system to enhance quantification in flow cytometry. We systematically assess the performance characteristics of this system with micron-sized polystyrene beads, addressing issues related to sample introduction, adsorption, and volume measurement. Results underscore the excellent analytical performance of the proposed method, characterized by high linearity and repeatability. We compare our approach to counting bead-based measurements, and while an approximate bias value was observed, the measured values were found to be similar between the methods, demonstrating its comparability and reliability. This method holds great promise for improving the accuracy and precision of particle quantification in flow cytometry, with implications for various fields including healthcare and environmental monitoring.
PubMed: 38926227
DOI: 10.1007/s00216-024-05387-6 -
ACS Applied Materials & Interfaces Jun 2024In recent years, the study of microplastics (MPs) and nanoplastics (NPs) and their effects on human health has gained significant attention. The impacts of NPs on lipid...
In recent years, the study of microplastics (MPs) and nanoplastics (NPs) and their effects on human health has gained significant attention. The impacts of NPs on lipid metabolism and the specific mechanisms involved remain poorly understood. To address this, we utilized high-throughput sequencing and molecular biology techniques to investigate how endoplasmic reticulum (ER) stress might affect hepatic lipid metabolism in the presence of polystyrene nanoplastics (PS-NPs). Our findings suggest that PS-NPs activate the PERK-ATF4 signaling pathway, which in turn upregulates the expression of genes related to lipid synthesis via the ATF4-PPARγ/SREBP-1 pathway. This activation leads to an abnormal accumulation of lipid droplets in the liver. 4-PBA, a known ER stress inhibitor, was found to mitigate the PS-NPs-induced lipid metabolism disorder. These results demonstrate the hepatotoxic effects of PS-NPs and clarify the mechanisms of abnormal lipid metabolism induced by PS-NPs.
PubMed: 38926154
DOI: 10.1021/acsami.4c04416 -
The Science of the Total Environment Jun 2024This research explored the effects of widely utilized nanomaterial graphene oxide (GO) and organic matter humic acid (HA) on the transport of microplastics under...
This research explored the effects of widely utilized nanomaterial graphene oxide (GO) and organic matter humic acid (HA) on the transport of microplastics under different ionic solution strengths in bare sand and iron oxide-coated sand. The results found transport of polystyrene microplastics (PS) did not respond to the presence of HA in sand that contains large amounts of iron oxide. Compared to bare quartz sand, ionic strength had little effect: <20 % of PS passed through Fe sand columns. There was a significant promotion of PS transport in the presence of GO, however, which can be attributed to the increased surface electronegativity of PS and steric hindrance. Moreover, GO combined with HA significantly promoted the transport of PS in the Fe sand, and transport further increased when the concentration of HA increased from 5 to 10 mg/L. Interestingly, the degree of this increase exactly corresponded to the change in the surface charge of the microplastics, demonstrating that electrostatic interaction dominated the PS transport. Further results indicated that co-existing pollutants had significant impacts on the transport of microplastics under various conditions by altering the surface characteristics of the plastic particles and the spatial steric hindrance within porous media. This research will offer insights into predicting the transport and fate of microplastics in complex environments.
PubMed: 38925391
DOI: 10.1016/j.scitotenv.2024.174270 -
The Science of the Total Environment Jun 2024Nanoplastics are difficult to remove from water using conventional flotation processes due to their stability and resistance to biodegradation. Here, polystyrene...
Nanoplastics are difficult to remove from water using conventional flotation processes due to their stability and resistance to biodegradation. Here, polystyrene nanoplastics (PSNPs) were selected as the object of study. In addition, chitosan (CTS), an environmentally friendly natural cationic polymer, was selected to modify the air flotation process to improve the separation of PSNPs using air flotation. Adding chitosan effectively enhanced the removal of PSNPs using air flotation from 3.1 % to 96.7 %. The residual concentration decreased from 9.69 mg/L to 0.33 mg/L. Removal of PSNPs by CTS-modified air flotation was maintained at 92.8 % even when the air flotation time was significantly shortened. The zeta potential alterations demonstrated robust electrostatic attraction within the CTS-modified air flotation process. The contact angle measurements indicated that incorporating CTS could enhance the hydrophobic interaction between bubbles and PSNPs. PSNPs particles around 100 nm agglomerated to form floating flocs with a particle size of more than 4500 nm. Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM) images confirmed the presence of tight adhesion between PSNPs and CTS, indicating the presence of bridging adsorption during the process. The major PSNPs removal mechanisms included electrostatic attraction, enhancement of hydrophobicity, and bridging adsorption. Increasing the aeration volume could improve the removal rate, but this improvement was finite. Weakly acidic and low ionic strength conditions favored PSNPs removal. The CTS-modified air flotation process showed great potential for PSNPs removal from real water bodies.
PubMed: 38925388
DOI: 10.1016/j.scitotenv.2024.174254 -
The Science of the Total Environment Jun 2024The settling behavior of microplastics (MPs) plays a pivotal role in their transport and fate in aquatic environments, but the dominant mechanisms and physics governing...
The settling behavior of microplastics (MPs) plays a pivotal role in their transport and fate in aquatic environments, but the dominant mechanisms and physics governing the settling of MPs in rivers remain poorly understood. To gain mechanistic insights into the velocity lag of MPs in an open-channel flume under different turbulent flow conditions, an experimental study was conducted using three types of MPs: polystyrene, cellulose acetate, and acrylic, of sphere-shaped particles with diameters ranging from 1 mm to 5 mm. A particle tracking technique was employed to record and analyze the MPs velocity within turbulent flows. The results showed a variation in the vertical settling velocity of MPs ω ranging from -26 % to +16 %, when compared to their counterparts in still water (ω). A new formula for the drag coefficient (C) of MP particles was developed by introducing the suspension number (u/ω). The developed C formula was used to calculate the resultant velocity lag V, with a mean relative error of 16 % compared with the measured values. Further, the study highlighted that the MPs with large Stokes numbers are mainly driven by their own inertia and turbulence has less influence on their settling behavior. This study is crucial for understanding the settling behavior of MPs in turbulent flows and developing their transport and fate models for MPs in riverine systems.
PubMed: 38925387
DOI: 10.1016/j.scitotenv.2024.174179 -
Journal of Colloid and Interface Science Jun 2024The petal effect is a well-known natural phenomenon in surface science and has served as inspiration for the creation of several materials with superhydrophobic...
HYPOTHESIS
The petal effect is a well-known natural phenomenon in surface science and has served as inspiration for the creation of several materials with superhydrophobic qualities and high adhesion. As surface roughness has a crucial role in these properties, being able to modulate it could help us design materials at will. Capillary penetration frustrates diffusion and promotes large contact angles as well as high adhesion.
EXPERIMENTS
Polystyrene surfaces were created using the spin-coating technique. By varying the polymer concentration, the surface roughness was modified. To determine the roughness parameters, atomic force microscopy was used. We recorded advancing and receding contact angles using water and glycerol as test liquids to study contact angle hysteresis, the work of adhesion and the apparent surface energy, which was determined with the Chibowski and Perea-Carpio method. For the purpose of elucidating the wetting states, captive bubble experiments were conducted.
FINDINGS
Using an easy method, we create polystyrene surfaces with both superhydrophobicity and strong adhesion, where the roughness area factor regulates wetting transitions from Cassie-Baxter to Wenzel. The receding contact angle suggests capillary penetration, which we demonstrate by captive bubble experiments. In addition, a link was found between the surface roughness and apparent surface energy.
PubMed: 38925063
DOI: 10.1016/j.jcis.2024.06.121 -
Macromolecular Rapid Communications Jun 2024Poly(3,4-ethylenedioxythiophene):polystyrene sulfonate (PEDOT:PSS), a conductive polymer, has gained popularity as the channel layer in organic electrochemical...
Poly(3,4-ethylenedioxythiophene):polystyrene sulfonate (PEDOT:PSS), a conductive polymer, has gained popularity as the channel layer in organic electrochemical transistors (OECTs) due to its high conductivity and straightforward processing. However, difficulties arise in controlling its conductivity through gate voltage, presenting a challenge. To address this issue, we employ aromatic amidine base, diazabicyclo[4.3.0]non-5-ene (DBN), to stabilize the doping state of the PEDOT chain through a reliable chemical de-doping process. Furthermore, the addition of the proton-penetrable material Nafion to the PEDOT:PSS channel layer induces phase separation between the substances. By utilizing a solution containing both PEDOT:PSS and Nafion as the channel layer of OECTs, we enhance the efficiency of ion movement into the channel from the electrolyte, resulting in improved OECT performance. The inclusion of Nafion in the OECTs' channel layer modifies ion movement dynamics, allowing for the adjustment of synaptic properties such as pulse-paired facilitation (PPF), memory level, short-term plasticity (STP), and long-term plasticity (LTP). This research aims to introduce new possibilities in the field of neuromorphic computing and contribute to biomimetic technology through the enhancement of electronic component performance This article is protected by copyright. All rights reserved.
PubMed: 38924243
DOI: 10.1002/marc.202400165