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Journal of Investigative and Clinical... Nov 2019To investigate the relationship between the microbiota of periodontal pockets (PP) and root canals (RC) in dogs submitted to experimental periodontal disease (ExPD).
AIM
To investigate the relationship between the microbiota of periodontal pockets (PP) and root canals (RC) in dogs submitted to experimental periodontal disease (ExPD).
METHODS
ExPD was induced by combining cotton and wire ligatures. After 125 days, microbiological samples were collected from PP and RC. Strains isolated from 19 teeth were submitted to DNA extraction, 16S rRNA gene amplification and gene sequencing. Pearson's χ - and Fisher's exact tests and McNemar's test were used when appropriate.
RESULTS
The number of species in PP was greater than in RC, with prevalence of obligate anaerobes and Gram-negative bacteria. In the PP predominated Fusobacterium necrophorum, Porphyromonas gingivalis, Prevotella loescheii, Campylobacter gracilis and Veillonella parvula. In the RC samples, 9 had microbial growth, with predominance of the following genera: Staphylococcus, Streptococcus and Neisseria. Eight genera were common to both sites in the same tooth. PP presented a greater number of species than the RC. No significant difference was observed in the species found in PP and RC in the same tooth.
CONCLUSION
Microbial composition of the RC could be modulated by the presence of periodontal disease, especially in cases of severe periodontal destruction. RC microbiota was less complex and diverse than the PP.
Topics: Animals; Dental Pulp Cavity; Dogs; Microbiota; Periodontal Diseases; Periodontal Pocket; RNA, Ribosomal, 16S
PubMed: 31313892
DOI: 10.1111/jicd.12439 -
Current Microbiology Sep 2019A novel Gram-negative, obligately anaerobic, non-motile, non-spore forming, and rod-shaped bacterium, designated strain JS262, was isolated from human subgingival plaque...
A novel Gram-negative, obligately anaerobic, non-motile, non-spore forming, and rod-shaped bacterium, designated strain JS262, was isolated from human subgingival plaque of periodontitis lesion and was characterized by polyphasic taxonomic analysis. Comparison of 16S ribosomal RNA gene (16S rDNA) sequence revealed that the strain belonged to the genus Prevotella. The percent similarity of 16S rDNA of strain JS262 was closest to those of Prevotella buccae ATCC 33574 (89.1%) and Prevotella shahii JCM 12083 (88.9%). The major fatty acids of strain JS262 were C (29.2%), iso-C (19.2%), and anteiso-C (16.9%). Complete genome of strain JS262 was 2,691,540 bp in length and the G+C content was 43.9 mol%. Average nucleotide identity and genome-to-genome distance values between strain JS262 and P. buccae ATCC 33574 or P. loescheii DSM 19665 were > 70.4% and > 30.1%, respectively. On the basis of these data, a novel Prevotella species is proposed: Prevotella koreensis sp. nov. The type strain of P. koreensis is JS262 (= KCOM 3155 = JCM 33298).
Topics: Bacterial Typing Techniques; Base Composition; Dental Plaque; Fatty Acids; Female; Genome, Bacterial; Humans; Middle Aged; Periodontitis; Phylogeny; Prevotella
PubMed: 31214821
DOI: 10.1007/s00284-019-01720-w