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International Journal of Systematic and... Feb 2020Two yellow-pigmented, Gram-stain-negative and rod-shaped bacterial strains, designated as RY24 and ZYY160, were isolated from rice. Results of phylogenetic analysis...
Two yellow-pigmented, Gram-stain-negative and rod-shaped bacterial strains, designated as RY24 and ZYY160, were isolated from rice. Results of phylogenetic analysis based on 16S rRNA gene sequences showed that strains RY24 and ZYY160 belonged to the genus , and the 16S rRNA gene sequence similarity was 100 % The DNA homology between the two strains was 99.7 %. The 16S rRNA and gene sequences of the two strains showed highest similarity values to CGMCC 1.3392 and DSM 15758 (sharing 99.31 and 94.34 %, respectively). The major fatty acids of two strains were identified as summed feature 8 (Cω7 and/or Cω6), C and summed feature 3 (Cω7 and/or Cω6), and the major respiratory quinone was identified as ubiquinone Q-9, which are typical chemotaxonomic features of members of the genus . The genomic DNA G+C contents of strains RY24 and ZYY160 were determined to be 64.25 and 64.21 mol%, respectively. The DNA-DNA relatedness and average nucleotide identity values between the two strains and their closely related type strains were below 36 and 90 %, which supported that RY24 and ZYY160 represent a novel species in the genus . Phylogenetic and chemotaxonomic evidence, together with phenotypic characteristics, showed that the two isolates constitute a novel species of the genus . The type strain is RY24 (JCM 33201=ACCC 61555), for which the name sp. nov. is proposed.
Topics: Bacterial Typing Techniques; Base Composition; China; DNA, Bacterial; Fatty Acids; Genes, Bacterial; Nucleic Acid Hybridization; Oryza; Phylogeny; Pseudomonas; RNA, Ribosomal, 16S; Sequence Analysis, DNA; Ubiquinone
PubMed: 31751195
DOI: 10.1099/ijsem.0.003852 -
Molecular Plant-microbe Interactions :... Feb 2020In endophytes, the abundance of genes coding for enzymes processing reactive oxygen species (ROS), including hydrogen peroxide (HO), argues for a crucial role of ROS...
In endophytes, the abundance of genes coding for enzymes processing reactive oxygen species (ROS), including hydrogen peroxide (HO), argues for a crucial role of ROS metabolism in plant-microbe interaction for plant colonization. Here, we studied HO metabolism of bread wheat ( L.) seeds and their microbiota during germination and early seedling growth, the most vulnerable stages in the plant life cycle. Treatment with hot steam diminished the seed microbiota, and these seeds produced less extracellular HO than untreated seeds. Using a culture-dependent approach, and genera were the most abundant epiphytes of dry untreated seeds. Incubating intact seedlings from hot steam-treated seeds with strains triggered HO production, whereas strains dampened HO levels, attributable to higher catalase activities. The genus was much less represented among seedling endophytes than genus , with other endophytic genera, including and , also possessing high catalase activities. Overall, our results show that certain bacteria of the seed microbiota are able to modulate the extracellular redox environment during germination and early seedling growth, and high catalase activity is proposed as a key trait of seed endophytes.
Topics: Germination; Hydrogen Peroxide; Oxidation-Reduction; Seedlings; Seeds; Triticum
PubMed: 31631769
DOI: 10.1094/MPMI-09-19-0248-R -
Clinical Case Reports Aug 2019Hidradenitis suppurativa (HS) is one of the neglected chronic inflammatory disorders which has not efficient treatment. These patients were susceptible to various...
Hidradenitis suppurativa (HS) is one of the neglected chronic inflammatory disorders which has not efficient treatment. These patients were susceptible to various infectious diseases because of their changes in immuneresponse. Also, HS pathogenesis remains unclear and its report can create novel insight into mechanism and pathogenesis of this infection. Moreover, given that different susceptibility patterns of spp this species should be identified to the species level; molecular methods are rapid, inexpensive, and reliable method for identification of infectious agents to the species level and appropriate treatment of infections.
PubMed: 31428378
DOI: 10.1002/ccr3.2265