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Microbiology Resource Announcements Jun 2024The genome sequence of multidrug-resistant RT01-5M1 strain isolated from Canadian farmed salmon was determined using Oxford nanopore and Illumina MiSeq sequencers. The...
The genome sequence of multidrug-resistant RT01-5M1 strain isolated from Canadian farmed salmon was determined using Oxford nanopore and Illumina MiSeq sequencers. The assembled chromosome was estimated at 5,699,993 bp in size, with two plasmids, 164,879 bp and 82,046 bp. The chromosome and smaller plasmid contained antimicrobial resistance genes.
PubMed: 38934593
DOI: 10.1128/mra.00153-24 -
Frontiers in Cellular and Infection... 2024spp. is a genus of bacteria that is known to be closely related to . It has been debated whether should be reclassified as a subgroup of . The aim of this study is to... (Comparative Study)
Comparative Study
PURPOSE
spp. is a genus of bacteria that is known to be closely related to . It has been debated whether should be reclassified as a subgroup of . The aim of this study is to compare clinical aspects of and , a species of that is known to be bacteriologically similar to spp.
METHODS
Using data collected at a tertiary care hospital in the United States, we identified 43 patients with infection and 1173 patients with infection. We compared patient demographics (age and sex), hospitalization status, isolation sites and antibiotic resistance profiles between the two species.
RESULTS
There was no significant difference in patient demographics between the two bacteria species. The proportions of intensive care unit (ICU) admission were higher among patients with infection (p=0.008). The most common site of isolation was urine for both species (39.5% of all patients with spp. vs. 59.3% for ). The second most common site of isolation was blood stream for spp. (23.3%) and respiratory tract for (10.8%). Except for the high proportion of resistant isolates of spp. for Trimethoprim/sulfamethoxazole, the antibiotic susceptibility profiles were similar between the two bacteria species. Both were susceptible to ciprofloxacin and meropenem.
CONCLUSION
While there are no significant differences in the patient demographics and antibiotic susceptibility profiles between spp. and , may cause more serious infection requiring ICU admissions. Also, may cause blood stream infection more frequently than .
Topics: Humans; Male; Klebsiella oxytoca; Female; Middle Aged; Aged; Enterobacteriaceae; Klebsiella Infections; Anti-Bacterial Agents; Enterobacteriaceae Infections; Microbial Sensitivity Tests; Adult; Tertiary Care Centers; Intensive Care Units; United States; Aged, 80 and over; Drug Resistance, Bacterial
PubMed: 38887491
DOI: 10.3389/fcimb.2024.1260212 -
Rhode Island Medical Journal (2013) Jun 2024Raoultella ornithinolytica is a rare, gram-negative environmental enterobacterium. Although infections in humans caused by R. ornithinolytica are uncommon, there are...
Raoultella ornithinolytica is a rare, gram-negative environmental enterobacterium. Although infections in humans caused by R. ornithinolytica are uncommon, there are increasing reports implicating it in urinary tract infections, hepatobiliary infections, and bacteremia, designating it as an emerging pathogen. Its habitat is primarily in aquatic environments and soil, with seafood frequently identified as a potential source of infection. While these infections have predominantly been described in immunocompromised patients previously, our case suggests that advanced age may be a significant risk factor. We describe a case of a 73-year-old man presenting with encephalopathy who then was found to have R. ornithinolytica bacteremia from a genitourinary source. Following antibiotic treatment, the infection resolved and the neurologic symptoms improved. To the best of our knowledge, this is the first documented case in the medical literature of R. ornithinolytica featuring a primary neurologic presentation.
Topics: Humans; Aged; Male; Enterobacteriaceae Infections; Anti-Bacterial Agents; Enterobacteriaceae; Brain Diseases; Bacteremia
PubMed: 38810006
DOI: No ID Found -
Frontiers in Microbiology 2024Seventeen Gram-negative, facultatively anaerobic bacterial strains were isolated from bleeding cankers of various broadleaf hosts and oak rhizosphere soil in Great...
Seventeen Gram-negative, facultatively anaerobic bacterial strains were isolated from bleeding cankers of various broadleaf hosts and oak rhizosphere soil in Great Britain. The strains were tentatively identified as belonging to the genus based on 16S rRNA gene sequencing. Multilocus sequence analysis (MLSA), based on four protein-encoding genes (, , , and ), separated the strains into three clusters within the genus clade. The majority of strains clustered with the type strain of , with the remaining strains divided into two clusters with no known type strain. Whole genome sequencing comparisons confirmed these two clusters of strains as belonging to two novel species which can be differentiated phenotypically from their current closest phylogenetic relatives. Therefore, two novel species are proposed: sp. nov. (type strain = BAC 10a-01-01 = LMG 33072 = CCUG 77096) and sp. nov. (type strain = TW_WC1a.1 = LMG 33073 = CCUG 77094).
PubMed: 38756725
DOI: 10.3389/fmicb.2024.1386923 -
Surgical Infections May 2024is an uncommon gram-negative organism found in the environment. The patient, an 81-year-old female who had undergone total cystectomy and bilateral ureteral stoma...
is an uncommon gram-negative organism found in the environment. The patient, an 81-year-old female who had undergone total cystectomy and bilateral ureteral stoma surgery, presented to the hospital with a fever. It was determined that was responsible for the bacteremia. Rapid identification of bacteria using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) in blood culture samples and appropriate antibacterial treatment was begun and the patient was discharged three days later. This case emphasizes the presence of a rare pathogen as the cause of bacteremia and underscores the importance of utilizing rapid methods for bacterial identification to establish an accurate diagnosis.
Topics: Humans; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Female; Bacteremia; Aged, 80 and over; Enterobacteriaceae; Enterobacteriaceae Infections; Blood Culture; Anti-Bacterial Agents
PubMed: 38696669
DOI: 10.1089/sur.2024.057 -
The Science of the Total Environment Jul 2024Ammonia‑nitrogen wastewater is one of the main pollutants in the current environment. Rapid detection of microorganisms resistant to ammonia‑nitrogen provides a...
Ammonia‑nitrogen wastewater is one of the main pollutants in the current environment. Rapid detection of microorganisms resistant to ammonia‑nitrogen provides a basis for bioremediation of ammonia‑nitrogen contaminated sites. This study uses electrochemical analysis for efficiently detecting of ammonia-resistant bacteria, utilizing a commercially available, low-cost screen-printed electrode (SPE) modified with agarose-based hydrogel (gel) or graphene oxide (GO). At the same time, the study employed electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV) to monitor bacterial growth, revealing Escherichia coli (E. coli) inhibition upon ammonia‑nitrogen addition, while Raoultella terrigena (RN1) and Pseudomonas (RN2) exhibit tolerance. The method provides sensitivity results in <45 min, which is significantly faster than traditional methods. RN1 and RN2 exhibit promising ammonia‑nitrogen removal rates, reaching up to 81 % and 92 %, respectively. This study aimed to develop an effective electrochemical method for rapidly detecting the sensitivity of microorganisms to ammonia‑nitrogen. The method offers advantages such as high speed, efficiency, and cost-effectiveness, potentially providing valuable microbial resources for mitigating ammonia nitrogen wastewater pollution.
Topics: Ammonia; Biosensing Techniques; Wastewater; Electrochemical Techniques; Nitrogen; Hydrogels; Bacteria; Water Pollutants, Chemical; Graphite; Escherichia coli
PubMed: 38692312
DOI: 10.1016/j.scitotenv.2024.172828 -
Scientific Reports Apr 2024The edible fungus industry is one of the pillar industries in the Yunnan-Guizhou Plateau, China. The expansion of the planting scale has led to the release of various...
The edible fungus industry is one of the pillar industries in the Yunnan-Guizhou Plateau, China. The expansion of the planting scale has led to the release of various mushroom residues, such as mushroom feet, and other wastes, which are not treated adequately, resulting in environmental pollution. This study investigated the ability of black soldier fly (Hermetia illucens L.) larvae (BSFL) to degrade mushroom waste. Moreover, this study analyzed changes in the intestinal bacterial community and gene expression of BSFL after feeding on mushroom waste. Under identical feeding conditions, the remaining amount of mushroom waste in Pleurotus ostreatus treatment group was reduced by 18.66%, whereas that in Flammulina velutipes treatment group was increased by 31.08%. Regarding gut microbial diversity, compared with wheat bran-treated control group, Dysgonomonas, Providencia, Enterococcus, Pseudochrobactrum, Actinomyces, Morganella, Ochrobactrum, Raoultella, and Ignatzschineria were the most abundant bacteria in the midgut of BSFL in F. velutipes treatment group. Furthermore, Dysgonomonas, Campylobacter, Providencia, Ignatzschineria, Actinomyces, Enterococcus, Morganella, Raoultella, and Pseudochrobactrum were the most abundant bacteria in the midgut of BSFL in P. ostreatus treatment group. Compared with wheat bran-treated control group, 501 upregulated and 285 downregulated genes were identified in F. velutipes treatment group, whereas 211 upregulated and 43 downregulated genes were identified in P. ostreatus treatment group. Using Kyoto Encyclopedia of Genes and Genomes and Gene Ontology enrichment analyses, we identified 14 differentially expressed genes (DEGs) related to amino sugar and nucleotide sugar metabolism in F. velutipes treatment group, followed by 12 DEGs related to protein digestion and absorption. Moreover, in P. ostreatus treatment group, two DEGs were detected for fructose and mannose metabolism, and two were noted for fatty acid metabolism. These results indicate that feeding on edible mushroom waste can alter the intestinal microbial community structure of BSFL; moreover, the larval intestine can generate a corresponding feedback. These changes contribute to the degradation of edible mushroom waste by BSFL and provide a reference for treating edible mushroom waste using BSFL.
Topics: Animals; Gastrointestinal Microbiome; Larva; Pleurotus; Agaricales; Biodegradation, Environmental; Diptera; Flammulina; Bacteria
PubMed: 38688964
DOI: 10.1038/s41598-024-60524-6 -
Cureus Mar 2024The case presentation discusses the clinical evaluation and treatment of a two-year-old female exhibiting symptoms such as dysuria, constipation, and foul-smelling...
The case presentation discusses the clinical evaluation and treatment of a two-year-old female exhibiting symptoms such as dysuria, constipation, and foul-smelling urine. Upon evaluation, the patient was found to be co-infected with Raoultella planticola and Escherichia coli. This co-infection poses unique challenges in diagnosis and treatment, as both pathogens may contribute to the manifestation of symptoms. The initial diagnosis of Raoultella planticola is notable, given its relatively rare occurrence and the potential for misdiagnosis. This case study contributes to our understanding of diagnosing and distinguishing symptoms at various stages of the illness, particularly in cases of co-infection. Following an initial urinalysis and urine culture confirming the presence of both pathogens, a 10-day course of antibiotics was prescribed. Subsequent examinations at Rady Children's Hospital-San Diego included kidney and abdomen imaging to rule out underlying issues. The co-infection underscores the importance of thorough diagnostic procedures and tailored treatment approaches. Additionally, it highlights the need for heightened awareness among healthcare providers regarding emerging pathogens and their potential clinical implications.
PubMed: 38686243
DOI: 10.7759/cureus.57262 -
Pharmacological Research Jun 2024Antibiotic related intestinal injury in early life affects subsequent health and susceptibility. Here, we employed weaned piglets as a model to investigate the...
Antibiotic related intestinal injury in early life affects subsequent health and susceptibility. Here, we employed weaned piglets as a model to investigate the protective effects of baicalin against early-life antibiotic exposure-induced microbial dysbiosis. Piglets exposed to lincomycin showed a marked reduction in body weight (p < 0.05) and deterioration of jejunum intestinal morphology, alongside an increase in antibiotic-resistant bacteria such as Staphylococcus, Dolosicoccus, Escherichia-Shigella, and Raoultella. In contrast, baicalin treatment resulted in body weights, intestinal morphology, and microbial profiles that closely resembled those of the control group (p > 0.05), with a significant increase in norank_f_Muribaculaceae and Prevotellaceae_NK3B31_group colonization compared with lincomycin group (p < 0.05). Further analysis through fecal microbial transplantation into mice revealed that lincomycin exposure led to significant alterations in intestinal morphology and microbial composition, notably increasing harmful microbes and decreasing beneficial ones such as norank_Muribaculaceae and Akkermansia (p < 0.05). This shift was associated with an increase in harmful metabolites and disruption of the calcium signaling pathway gene expression. Conversely, baicalin supplementation not only counteracted these effects but also enhanced beneficial metabolites and regulated genes within the MAPK signaling pathway (MAP3K11, MAP4K2, MAPK7, MAPK13) and calcium channel proteins (ORA13, CACNA1S, CACNA1F and CACNG8), suggesting a mechanism through which baicalin mitigates antibiotic-induced intestinal and microbial disturbances. These findings highlight baicalin's potential as a plant extract-based intervention for preventing antibiotic-related intestinal injury and offer new targets for therapeutic strategies.
Topics: Animals; Flavonoids; Anti-Bacterial Agents; Gastrointestinal Microbiome; Swine; MAP Kinase Signaling System; Lincomycin; Mice; Dysbiosis; Male; Intestines
PubMed: 38663526
DOI: 10.1016/j.phrs.2024.107194 -
The Science of the Total Environment Jun 2024To obtain a multifunctional bacterium that can effectively degrade polyethylene (PE) and sulfonamide antibiotics (SAs), PE and SAs were selected as the primary research...
To obtain a multifunctional bacterium that can effectively degrade polyethylene (PE) and sulfonamide antibiotics (SAs), PE and SAs were selected as the primary research objects. Multifunctional degrading bacteria were isolated and screened from an environment in which plastics and antibiotics have existed for a long time. An efficient degrading strain, Raoultella sp., was screened by measuring the degradation performance of PE and SAs. We analyzed the changes in the microbial community of indigenous bacteria using 16S rRNA. After 60 d of degradation at 28 °C, the Raoultella strain to PE degradation rate was 4.20 %. The SA degradation rates were 96 % (sulfonathiazole, (ST)), 86 % (sulfamerazine, (SM)), 72 % (sulfamethazine, (SM2)) and 64 % (sulfamethoxazole, (SMX)), respectively. This bacterium increases the surface roughness of PE plastic films and produces numerous gullies, pits, and folds. In addition, after 60 d, the contact angle of the plastic film decreased from 92.965° to 70.205°, indicating a decrease in hydrophobicity. High-throughput sequencing analysis of the degrading bacteria revealed that the Raoultella strain encodes enzymes involved in PE and SA degradation. The results of this study not only provide a theoretical basis for further study of the degradation mechanism of multifunctional and efficient degrading bacteria but also provide potential strain resources for the biodegradation of waste plastics and antibiotics in the environment.
Topics: Biodegradation, Environmental; Anti-Bacterial Agents; Soil Pollutants; Polyethylene; Soil Microbiology; RNA, Ribosomal, 16S; Soil; Bacteria
PubMed: 38649045
DOI: 10.1016/j.scitotenv.2024.172619