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Plants (Basel, Switzerland) Jun 2021Antimicrobials are widely applied in aquaculture for treatment of infectious diseases in fish. The increased antimicrobial resistance of fish pathogens to conventional...
Antimicrobials are widely applied in aquaculture for treatment of infectious diseases in fish. The increased antimicrobial resistance of fish pathogens to conventional antimicrobial treatment highlights the need for research on the antibacterial properties of natural products-in this case, essential oils (EOs). The aim of the present study was to detect the antimicrobial activity of the essential oils on pathogenic microorganisms found in freshwater fish. Freshwater fish isolates of spp., spp., spp., spp., spp., spp., spp., and spp. were tested for antimicrobial resistance and antimicrobial activity against 14 commercially available essential oils. Antimicrobial resistance was identified in spp. isolates against cefepime and ciprofloxacin; while all , , and isolates were fully susceptible. All tested EOs revealed antimicrobial activity against the tested freshwater fish isolates at different extents. exhibited strong antimicrobial activity against spp. (3.12 μL/mL), spp. (0.78-1.56 μL/mL), and spp. with the MIC method. EOs of and showed strong antibacterial activity against spp. and spp. (6.25 μL/mL). The study shows the antimicrobial activity of EOs against the most relevant freshwater fish pathogens and indicates the application opportunities in aquaculture.
PubMed: 34206270
DOI: 10.3390/plants10071265 -
Microbial Pathogenesis Sep 2021One of the major factors that affect the total production of fisheries is the declining number of catches and aquaculture production due to the high pathogenicity in...
One of the major factors that affect the total production of fisheries is the declining number of catches and aquaculture production due to the high pathogenicity in aquatic environment. This enforces the need to find anti-pathogenic agents that could solve the problem. In addition, the application of potential Bacillus cereus SN7 and Vagococcus fluvialis CT21 isolated and identified from the sea water of Siak, Riau, Indonesia need to be optimally exploited. The aim of this study, therefore, is to determine the component of bioactive compounds present in Bacillus cereus SN7 and Vagococcus fluvialis CT21, and also to explore their intrinsic potential as a biological control agent in fisheries, especially for inhibiting the growth of pathogenic bacteria (Vibrio alginolyticus, Aeromonas hydrophila and Pseudomonas aeruginosa). The method used was experimental, where the ethyl acetate crude extracts of both samples were analyzed for their phytochemical content, followed by thin layer chromatography analysis and Liquid Chromatography Mass Spectrometry. In addition, anti-pathogenic activity test was performed using the Kirby-Bauer method, minimum inhibitory and bactericidal concentration analysis. The results showed alkaloids, flavonoids, and saponins were the potential bioactive components in the crude extracts of Bacillus cereus SN7 and Vagococcus fluvialis CT21. Furthermore, the anti-pathogenic activity test demonstrated the ability for both bacteria to inhibit three types of pathogens with the following inhibitory zone values: Vibrio alginolyticus (10-11 mm), Aeromonas hydrophila (8-12 mm), and Pseudomonas aeruginosa (8-10 mm). In conclusion secondary metabolite compounds produced by Bacillus cereus SN7 and Vagococcus fluvialis CT21 possess the capacity to inhibit pathogenic bacteria. Hence, both samples are potential candidates for anti-pathogen development, especially in fisheries.
Topics: Animals; Anti-Bacterial Agents; Bacillus cereus; Enterococcaceae; Fishes; Microbial Sensitivity Tests; Pseudomonas aeruginosa
PubMed: 34186116
DOI: 10.1016/j.micpath.2021.105062 -
Proceedings of the National Academy of... Jun 2021While it is well recognized that the environmental resistome is global, diverse, and augmented by human activities, it has been difficult to assess risk because of the...
While it is well recognized that the environmental resistome is global, diverse, and augmented by human activities, it has been difficult to assess risk because of the inability to culture many environmental organisms, and it is difficult to evaluate risk from current sequence-based environmental methods. The four most important criteria to determine risk are whether the antibiotic-resistance genes (ARGs) are a complete, potentially functional complement; if they are linked with other resistances; whether they are mobile; and the identity of their host. Long-read sequencing fills this important gap between culture and short sequence-based methods. To address these criteria, we collected feces from a ceftiofur-treated cow, enriched the samples in the presence of antibiotics to favor ARG functionality, and sequenced long reads using Nanopore and PacBio technologies. Multidrug-resistance genes comprised 58% of resistome abundance, but only 0.8% of them were plasmid associated; fluroquinolone-, aminoglycoside-, macrolide-lincosamide-streptogramin (MLS)-, and β-lactam-resistance genes accounted for 2.7 to 12.3% of resistome abundance but with 19 to 78% located on plasmids. A variety of plasmid types were assembled, some of which share low similarity to plasmids in current databases. Enterobacteriaceae were dominant hosts of antibiotic-resistant plasmids; physical linkage of extended-spectrum β-lactamase genes (, , , and ) was largely found with aminoglycoside-, MLS-, tetracycline-, trimethoprim-, phenicol-, sulfonamide-, and mercury-resistance genes. A draft circular chromosome of was assembled; it carries MLS-, tetracycline- (including and on an integrative conjugative element), and trimethoprim-resistance genes flanked by many transposase genes and insertion sequences, implying that they remain transferrable.
Topics: Animals; Anti-Bacterial Agents; Base Sequence; Cattle; Drug Resistance, Microbial; Environmental Microbiology; Feces; Gene Regulatory Networks; Genes, Bacterial; Genetic Linkage; Genetic Variation; Host Specificity; Microbiota; Phylogeny; Plasmids; Sequence Analysis, DNA
PubMed: 34161269
DOI: 10.1073/pnas.2024464118 -
Food Science and Biotechnology Mar 2021The effects of vacuum package combined with 0.1% ε-polylysine and 0.2% rosemary extract (V + RP) on the quality attributes and microbial communities of large yellow...
The effects of vacuum package combined with 0.1% ε-polylysine and 0.2% rosemary extract (V + RP) on the quality attributes and microbial communities of large yellow croaker () during ice storage were investigated. The quality was evaluated by chemical characteristics (total volatile basic nitrogen (TVB-N), K-value and biogenic amines (BAs)), microbiological indexes (Total viable counts (TVC), bacteria counts, bacteria counts, Psychrophilic bacteria counts (PBC)), changes in microbial composition were analyzed using high-throughput sequencing. Results showed that the increase of TVB-N, K-value, microorganisms and BAs could be inhibited by V + RP. and were detected in all samples. increases rapidly in the middle of storage. and were related to the decomposition of ATP, the formation of BAs, and TVB-N, respectively. In conclusion, V + RP presented the optimal effects, which could extend the shelf life of large yellow croaker for another 9 days compared with the control.
PubMed: 33868757
DOI: 10.1007/s10068-021-00880-5 -
Archives of Microbiology Aug 2021The continual increase in resistance to antibacterial drugs has become a major public health problem, and their indiscriminate use in agriculture, aquaculture, and the...
The continual increase in resistance to antibacterial drugs has become a major public health problem, and their indiscriminate use in agriculture, aquaculture, and the treatment of human and animal diseases has severely contributed to the occurrence and spread of multidrug resistance genes. This study phylogenetically characterized multidrug-resistant bacteria isolated from seafood cocktails. Seafood cocktail dishes from 20 establishments on public roads were sampled. Samples were grown on TCBS agar and blood agar. Forty colonies with different macro- and microscopic characteristics were isolated. The 16S rRNA gene V4 and V6 hypervariable regions were amplified, sequenced and phylogenetically analyzed. Antibacterial drug resistance was determined by disk diffusion assay. Isolated bacteria were identical to species of the genera Enterococcus, Proteus, Vibrio, Staphylococcus, Lactococcus, Vagococcus, Micrococcus, Acinetobacter, Enterobacter, and Brevibacterium, with 75-100% presenting resistance or intermediate resistance to dicloxacillin, ampicillin, and penicillin; 50-70% to cephalosporins; 30-67.5% to amikacin, netilmicin and gentamicin; 40% to nitrofurantoin and other antibacterial drugs; 25% to chloramphenicol; and 2.5% to trimethoprim with sulfamethoxazole. In general, 80% of the bacteria showed resistance to multiple antibiotics. The high degree of bacterial resistance to antibacterial drugs indicates that their use in producing raw material for marine foods requires established guidelines and the implementation of good practices.
Topics: Bacteria; Drug Resistance, Multiple, Bacterial; Phylogeny; Seafood
PubMed: 33864113
DOI: 10.1007/s00203-021-02319-1 -
Journal of Microbiology (Seoul, Korea) Apr 2021
Erratum to: Description of Vagococcus coleopterorum sp. nov., isolated from the intestine of the diving beetle, Cybister lewisianus, and Vagococcus hydrophili sp. nov., isolated from the intestine of the dark diving beetle, Hydrophilus acuminatus, and emended description of the genus Vagococcus.
PubMed: 33779955
DOI: 10.1007/s12275-021-0697-4 -
G3 (Bethesda, Md.) Jan 2021While the gram-positive bacterium Vagococcus fluvialis has been isolated from the environment as well as fish, birds, and mammals, very little is known about the...
While the gram-positive bacterium Vagococcus fluvialis has been isolated from the environment as well as fish, birds, and mammals, very little is known about the species. V. fluvialis is believed to be a probiotic in fishes. However, within mammals, it is more frequently isolated from infectious tissue, including on rare occasions human and livestock lesions. Prior to the study described here, V. fluvialis had never been found in healthy bovine animals. Here, we present the complete genomes of V. fluvialis UFMG-H6, UFMG-H6B, and UFMG-H7, novel strains isolated from urine samples from healthy bovine females. These are the first genomes of mammalian isolates and the first description of V. fluvialis from urine. The genomes did not encode for any known virulence genes, suggesting that they may be commensal members of the urine microbiota.
Topics: Animals; Cattle; Enterococcaceae; Female; Fishes; Virulence
PubMed: 33561240
DOI: 10.1093/g3journal/jkaa034 -
3 Biotech Feb 2021An innovative investigation was undertaken into the abundance and diversity of high antibiotic-resistant bacteria in aquaculture waters in Shandong Province, China,...
UNLABELLED
An innovative investigation was undertaken into the abundance and diversity of high antibiotic-resistant bacteria in aquaculture waters in Shandong Province, China, through cumulation incubation, PCR amplification of 16S rDNA, and high-throughput sequencing. The results showed that and were abundant in the aquaculture waters, whereas other phylum including , , and had relatively lower abundance. Our studies revealed the presence of different bacteria in different locations in the aquaculture waters, most of which were resistant to multiple antibiotics. That is, the same microbial species from the same aquaculture wastewater can resist different antibiotics. Altogether, a considerable portion of the microbial community were found to be multi-drug resistant. It is essential that the spread of the antibiotic-resistant bacteria is controlled so that the distribution of antibiotic resistance genes to other environments is avoided.
SUPPLEMENTARY INFORMATION
The online version contains supplementary material available at 10.1007/s13205-021-02656-4.
PubMed: 33552832
DOI: 10.1007/s13205-021-02656-4 -
Biotechnology Reports (Amsterdam,... Mar 2021Lactic acid bacteria (LAB) isolated from freshwater fish (hatcheries and captures) from Paraná river (Argentina) were analyzed by using culture-dependent approaches....
Lactic acid bacteria (LAB) isolated from freshwater fish (hatcheries and captures) from Paraná river (Argentina) were analyzed by using culture-dependent approaches. The species belonging to (.) , . , . , . and (.) were identify as predominant by RAPD-PCR and 16 s rRNA gene sequencing. (H-17, S-30, B-42 and S-44) grew in raw fish extract and slightly reduced the medium pH (5.81-5.91). These strains exhibited moderate fish sarcoplasmic protein degradation (≤ 73 %) releasing small peptides and free amino acids, being alanine, glycine, asparagine and arginine concentrations increased in a higher extent (17.84, 1.47, 1.26 and 0.47 mg/100 mL, respectively) by S-44 strain at 96 h incubation. Interestingly H-17 was able to inhibit Results suggest that these strains would contribute to the development of new safe and healthy fishery products with improved nutritional and sensory characteristics.
PubMed: 33489790
DOI: 10.1016/j.btre.2021.e00589 -
Journal of Applied Microbiology Aug 2021Evaluation of the antibacterial activity of cultivable bacteria associated with the marine sponges Hymeniacidon perlevis and Halichondria panicea against...
AIMS
Evaluation of the antibacterial activity of cultivable bacteria associated with the marine sponges Hymeniacidon perlevis and Halichondria panicea against multi-drug-resistant Staphylococcus aureus.
METHODS AND RESULTS
One hundred and fourteen bacterial isolates were recovered from H. perlevis and H. panicea. Antibacterial action was demonstrated by 70% of the isolates against reference strain Staphylococcus aureus ATCC 29213 and by 31·6% against Pseudomonas aeruginosa ATCC 27853 in agar overlay assays. Antibacterial potential was further analysed against 36 multi-drug-resistant hospital Staphylococcus aureus strains with diverse resistance profiles. Among the 80 isolates positive against S. aureus ATCC 29213, 76·3% were active against at least one clinical S. aureus pathogen and 73·6% inhibited one or more methicillin-resistant (MRSA) and vancomycin non-susceptible S. aureus strains. In addition, 41·3% inhibited all vancomycin nonsusceptible MRSA strains.
CONCLUSIONS
Culturable bacteria associated to H. perlevis and H. panicea are promising sources of antibacterial compounds of great pharmaceutical interest.
SIGNIFICANCE AND IMPACT OF THE STUDY
This study was the first to explore the antibacterial potential of culturable bacteria associated with the marine sponges H. perlevis and H. panicea against MDR bacteria. This is the first report of antibacterial activity by Aquimarina, Denitrobaculum, Maribacter and Vagococcus isolates against MDR S. aureus strains, including vancomycin nonsusceptible and methicillin-resistant ones, against which new antibiotics are urgently needed.
Topics: Animals; Anti-Bacterial Agents; Bacteria; Methicillin Resistance; Methicillin-Resistant Staphylococcus aureus; Porifera; Pseudomonas aeruginosa; Staphylococcus aureus; Vancomycin
PubMed: 33421270
DOI: 10.1111/jam.14999