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Microbial Ecology May 2023Bonelli's eagle (Aquila fasciata) is an endangered raptor species in Europe, and trichomonosis is one of the menaces affecting chicks at nest. In this paper, we attempt...
Bonelli's eagle (Aquila fasciata) is an endangered raptor species in Europe, and trichomonosis is one of the menaces affecting chicks at nest. In this paper, we attempt to describe the oral microbiome of Bonelli's eagle nestlings and evaluate the influence of several factors, such as captivity breeding, Trichomonas gallinae infection, and the presence of lesions at the oropharynx. The core oral microbiome of Bonelli's eagle is composed of Firmicutes, Bacteroidota, Fusobacteria and Proteobacteria as the most abundant phyla, and Megamonas and Bacteroides as the most abundant genera. None of the factors analysed showed a significant influence on alfa diversity, but beta diversity was affected for some of them. Captivity breeding exerted a high influence on the composition of the oral microbiome, with significant differences in the four most abundant phyla, with a relative increase of Proteobacteria and a decrease of the other three phyla in comparison with chicks bred at nest. Some genera were more abundant in captivity bred chicks, such as Escherichia-Shigella, Enterococcus, Lactobacillus, Corynebacterium, Clostridium and Staphylococcus, while Bacteroides, Oceanivirga, Peptostreptococcus, Gemella, Veillonella, Mycoplasma, Suttonella, Alloscardovia, Varibaculum and Campylobacter were more abundant in nest raised chicks. T. gallinae infection slightly influenced the composition of the microbiome, but chicks displaying trichomonosis lesions had a higher relative abundance of Bacteroides and Gemella, being the last one an opportunistic pathogen of abscess complications in humans. Raptor's microbiomes are scarcely studied. This is the first study on the factors that influence the oral microbiome of Bonelli's eagle.
Topics: Animals; Humans; Trichomonas; Eagles; Europe
PubMed: 35385973
DOI: 10.1007/s00248-022-02002-y -
International Journal of Systematic and... Aug 2021Two rod-shaped and Gram-stain-positive bacteria (strains C64 and C62) were isolated in 2020 from faeces of greater white-fronted geese () from Poyang Lake, PR China....
Two rod-shaped and Gram-stain-positive bacteria (strains C64 and C62) were isolated in 2020 from faeces of greater white-fronted geese () from Poyang Lake, PR China. Their optimal growth conditions were at 37 °C, pH 7.0 and with 0.5 % (w/v) NaCl. The two isolates showed a highest 16S rRNA gene sequence similarity to DSM 19116 (92.1 %). Phylogenetic/phylogenomic analyses indicated that strains C64 and C62 clustered independently in the vicinity of the genera , and within the family , but could not be classified clearly as members of any of these known genera. The average amino acid identity values between our isolates and available genomes of members of the family were around the genus threshold value (45-65 %). The major cellular fatty acids of the strains were Cω and C. The predominant polar lipids were phosphatidylinositol, phosphatidylglycerol, phosphatidylcholine, diacylglycerol, triacylglycerol and cardiolipin. The amino acid composition of peptidoglycan contained alanine, glutamic acid and glycine. The major respiratory menaquinones were MK-8(H) and MK-9(H). The whole cell sugars included galactose, arabinose and glucose. On the basis of the results of the 16S rRNA gene sequences comparison, whole-genome phylogenomic analysis, phenotypic and chemotaxonomic characteristics, we propose that strains C64 and C62 represent a novel species belonging to a novel genus within the family , for which the name gen. nov., sp. nov. is proposed. The type strain is C64 (=CGMCC 1.18410=GDMCC 1.1969=KCTC 49511=KACC 22143).
Topics: Actinomycetaceae; Animals; Bacterial Typing Techniques; Base Composition; China; DNA, Bacterial; Fatty Acids; Feces; Geese; Phospholipids; Phylogeny; RNA, Ribosomal, 16S; Sequence Analysis, DNA; Vitamin K 2
PubMed: 34431769
DOI: 10.1099/ijsem.0.004978 -
New Microbes and New Infections Nov 2019sp. nov. strain Marseille-P2802 (= CSUR P2802 = DSM 103074) is a new species within the genus in the phylum Actinobacteria that was isolated from the urine of a...
sp. nov. strain Marseille-P2802 (= CSUR P2802 = DSM 103074) is a new species within the genus in the phylum Actinobacteria that was isolated from the urine of a 59-year-old man treated with chronic haemodialysis for diabetic nephropathy.
PubMed: 31641508
DOI: 10.1016/j.nmni.2019.100591 -
New Microbes and New Infections Sep 2019Culturomics studies the microbial variety of the human microbiome by combining diversified culture conditions, matrix-assisted laser desorption/ionization time-of-flight...
Culturomics studies the microbial variety of the human microbiome by combining diversified culture conditions, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and 16S rRNA gene identification. This study identifies three putative new bacterial species: sp. nov. strain Marseille-P5647, sp. nov. strain Marseille-P5644 and sp. nov. strain Marseille-P5995, which we describe according to the concept of taxonogenomics.
PubMed: 31367386
DOI: 10.1016/j.nmni.2019.100585 -
New Microbes and New Infections Sep 2019sp. nov. strain Marseille-P3369 (= CSURP3369) is a new species from the order that has been isolated from a fresh stool sample of a healthy French woman.
sp. nov. strain Marseille-P3369 (= CSURP3369) is a new species from the order that has been isolated from a fresh stool sample of a healthy French woman.
PubMed: 31333849
DOI: 10.1016/j.nmni.2019.100575