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Journal of Conservative Dentistry and... Mar 2024Several designs of access cavity have been evolved in the recent past with the concept of minimal tooth tissue removal which would improve the root canal treated teeth...
BACKGROUND
Several designs of access cavity have been evolved in the recent past with the concept of minimal tooth tissue removal which would improve the root canal treated teeth fracture resistance.
AIM
To investigate the effect of conservative design access cavity during the instrumentation of maxillary molar root canals.
MATERIALS AND METHODS
Eighty noncarious maxillary molars were assigned to the traditional and conservative access groups ( = 40 each). After designated access preparations, the teeth were immersed in Lugols's solution for staining the pulp tissue. Root canal instrumentation was performed with TruNatomy file system. Pre- and postinstrumentation nano-computed tomography (CT) images were taken and reconstructed using CT-An software. Root canals volumetric analysis was done with CT-Vol software. The analysis of the data was dealt with Shapiro-Wilk test and independent -test.
RESULTS
The volume of pulp canal space before and after instrumentation changed significantly between the traditional and conservative access design groups, according to an independent -test. In comparison, the mean volume of dentin removed was much larger in the TAC group ( = 0.0016). The independent -test manifests difference significantly between traditional endodontic access cavity (TAC) and conservative access cavity (CAC) with percentage of unprepared canal walls. The mean percentage of unprepared area was significantly lesser in TAC group as compared to CAC group ( = 0.0022).
CONCLUSION
The volume of dentin removed was greater in TAC than with the CAC design. The amount of untouched canal wall area was significantly higher in conservative access design than with the traditional access design group.
PubMed: 38634036
DOI: 10.4103/JCDE.JCDE_272_23 -
Scientific Reports May 2022Here we utilize chemical ecology as a tool to manipulate the biological system of a small, but highly venomous to humans, cubozoan jellyfish, Carukia barnesi. We...
Here we utilize chemical ecology as a tool to manipulate the biological system of a small, but highly venomous to humans, cubozoan jellyfish, Carukia barnesi. We trialled a range of chemical reagents including indole compounds, 9-cis-retinoic acid and lugols solution to induce metamorphosis between the polyp and medusa life stages. An optimum method was determined resulting in a 90% metamorphosis rate to healthy medusa by exposing the polyps to 1 μM of 5-methoxy-2-methylindole for 24 h. Of note is that chemical exposure time significantly impacts health and metamorphosis rates in this species. We also present a theoretical mechanism for the chemical/biological interactions occurring during metamorphosis. This is a significant methodological advancement which now enables rearing of this animal en mass in aquaria-a world first for this species-which will subsequently supply and facilitate venom research into this understudied jellyfish.
Topics: Animals; Cnidaria; Cubozoa; Ecology; Metamorphosis, Biological; Scyphozoa
PubMed: 35641559
DOI: 10.1038/s41598-022-12812-2