-
Heliyon Jun 2024Sour orange () seeds are typically discarded by juice processors as waste. This study aimed to extract protein isolates, produce hydrolysates from de-oiled sour orange...
Sour orange () seeds are typically discarded by juice processors as waste. This study aimed to extract protein isolates, produce hydrolysates from de-oiled sour orange seeds (SOS), and characterize their physicochemical properties. Previous studies have described methods to obtain protein isolates and hydrolysates from agricultural residues. However, there is limited data on the SOS. This research characterized protein isolates and hydrolysates from SOS, emphasizing yield, purity, and amino acid composition. Protein isolates were extracted using borate saline buffer, saline, and distilled water. Enzymatically hydrolysis was conducted using Protamex® (a commercial protease) at concentrations ranging from 0.2 to 5 g enzyme/100g protein isolate. Differential scanning calorimetry, electrophoresis, and FT-IR spectroscopy were utilized to characterize the isolates and hydrolysates. Data showed that using 5 % saline resulted in protein extraction with a yield and purity of 30 and 86 %, respectively. DSC analysis revealed that the denaturation temperature of the protein isolate was 68 °C, while the hydrolysates exhibited structural instability, as indicated by a decrease in enthalpy change compared to the isolate. The protein isolate had a 76° contact angle. The amino acid profile showed a significant presence of glutamic acid (130.530 mg/g) and arginine (70.210 mg/g). Electrophoresis analysis exhibited four major bands of the protein. The bands' intensity decreased, and new bands appeared after hydrolysis. The enzyme hydrolysis was confirmed using the O-phthaldialdehyde method and FTIR. Findings revealed that based on the free amine group quantity, the hydrolysate obtained using 5 g enzyme/100g protein isolate was 14.220 ± 0.299 μmol/mg protein. The study concluded that sour orange seeds are a good source of protein, with protein isolates and hydrolysates exhibiting desirable characteristics. More research needs to be conducted to acquire further information about their functional properties and potential applications.
PubMed: 38952368
DOI: 10.1016/j.heliyon.2024.e32503 -
Heliyon Jun 2024Population growth and climate change challenge our food and farming systems and provide arguments for an increased intensification of agriculture. Organic farming has...
Population growth and climate change challenge our food and farming systems and provide arguments for an increased intensification of agriculture. Organic farming has been seen as a promising option due to its eco-friendly approaches during production. However, weeds are regarded as the major hindrance to effective crop production which varies depending on the type of crop and spacing. Their presence leads to reduced yield, increase in harvest cost and lower the qualities of some produce. Thus, weed management is a key priority for successful crop production. Therefore, we conducted a meta-analysis from published studies to quantify possible differences on weed density, diversity and evenness in organic and conventional farming systems and best intervention for weed management in organic farming system. Data included were obtained from 32 studies where 31 studies with 410 observations were obtained for weed density, 15 studies with 168 observations for diversity, and 5 studies with 104 observations for evenness. Standard deviation of mean was obtained from the studies, log transformed using natural logarithms and the effect size pooled using standardized mean difference (SMD). Publication bias was determined through funnel plot. Results showed that organic farming has significant higher weed density , diversity , and evenness compared to conventional farming. Despite so, diversified crop rotation has been proved to reduce weed density in organic farming by up to 49 % while maize-bean intercropping decrease densities of ssp, ssp and C ssp compared with monocropping. Use of mulch after one hand weeding was found to control up to 98 % of weeds and use of cover crop between 24 % and 85 % depending on the type of the cover crop. The study results show that organic farming encourages high weed density, diversity and evenness but use of the integrated approaches can help to maintain weed density at a manageable level.
PubMed: 38952364
DOI: 10.1016/j.heliyon.2024.e32761 -
Heliyon Jun 2024Based on a specimen preserved in the snakebite treatment center in eastern Nepal, this study aims to analyse brief life history (i.e., litter size, parturition date,...
Based on a specimen preserved in the snakebite treatment center in eastern Nepal, this study aims to analyse brief life history (i.e., litter size, parturition date, habitat preference) of , effects of its envenoming, and anthropogenic threat on its populations. One euthanized gravid female collected alive from eastern Nepal by a snakebite victim was examined. The gravid female gave birth to 23 litters on July 11, 2012. This parturition suggested high reproductive potential. Its bite to a man walking on the road caused no ill-effects. However, the use of tourniquet as a part of prehospital care might be detrimental or be obstacle for in-hospital care of snakebite. The conflicts between and humans at roads and human-activity areas are inevitable across the distribution ranges of this species. The continued anthropogenic impact can threaten its populations. This study finding can be a basis for assessing conservation status and options for its conservation as well as prehospital care and prevention of its bites.
PubMed: 38952358
DOI: 10.1016/j.heliyon.2024.e32577 -
Current Developments in Nutrition Jun 2024Fruits and vegetables (FV) are a critical source of nutrients, yet children in the United States are not meeting the Dietary Guidelines for Americans (DGA). The monthly...
Evaluating the Association of the Increase in the WIC Cash Value Benefit on the Diversity of MyPlate Fruits and Vegetables Redeemed and Consumed By Children in Low-Income Households.
BACKGROUND
Fruits and vegetables (FV) are a critical source of nutrients, yet children in the United States are not meeting the Dietary Guidelines for Americans (DGA). The monthly FV cash value benefit (CVB) included in the Special Supplemental Nutrition Program for Women, Infants, and Children (WIC)'s food package to support child FV intake (FVI) received a substantial increase for economic relief during the COVID-19 pandemic.
OBJECTIVES
To evaluate how an expansion of the monthly WIC CVB to purchase FV for WIC children ages 1-4 y is associated with diversity in FV redeemed, and how changes in redeemed FV are related to FVI.
METHODS
Caregivers representing 1463 WIC-participating children recruited from Los Angeles County, California, completed surveys during the CVB augmentation (T1: CVB = $9/mo; T2 = $35/mo; T3 = $24/mo). Redeemed price look-up codes (PLUs), corresponding to a food item, were assigned to its corresponding MyPlate FV group. Multivariable generalized estimating equation regression models assessed changes in amount and diversity of FV redemption across MyPlate groups and associations between changes in FV diversity and changes in FVI.
RESULTS
Slightly over half of all households were food insecure (55%), half of the children were female (52%), and most were Hispanic (78%). Compared with T1, significant increases in the number of PLUs and dollars redeemed were observed in most MyPlate FV groups. From T1 to T2, significant increases in diversity scores were observed for total fruit (β: 1.6 pts; 95% confidence interval [CI]: 1.4, 1.7), total vegetable (β: 3.6 pts; 95%CI: 3.4, 3.9), and total FV (β:7.8 pts; 95%CI: 7.4, 8.2). Similarly, increases in diversity score were observed at T3 compared with T1. Changes in FV diversity redeemed were not associated with changes in FVI.
CONCLUSIONS
During the CVB augmentation, WIC participants redeemed a greater amount and variety of FV according to DGA MyPlate recommendations, supporting its permanent increase.
PubMed: 38952351
DOI: 10.1016/j.cdnut.2024.103778 -
Veterinary Medicine and Science Jul 2024Indigenous village chickens, or (IVCs), significantly contribute to rural livelihoods. Although natural selection has a disproportionate impact on the genetic structure...
BACKGROUND
Indigenous village chickens, or (IVCs), significantly contribute to rural livelihoods. Although natural selection has a disproportionate impact on the genetic structure of IVCs, farmers have developed locally tailored breeding practices to get the most out of their flocks.
OBJECTIVES
Small-scale farmers' insights on trait breed preferences and family flock breeding practices are presented in this cross-sectional study.
METHODS
A cross-sectional study was conducted in two agroecological zones using face-to-face individual interviews with 119 general informants.
RESULTS
Farmers prefer IVCs because they are multipurpose birds. Unlike policymakers, who usually underestimate the importance of IVCs, small-scale farmers acknowledge the coexistence of local and commercial chickens. Only 15.7% of farmers recruited homegrown cocks, whereas 47.9% outsourced breeding cocks from local markets and 36.4% from neighbours (χ-squared = 15.976, df = 2, p = 0.0003395). About 49.2% of small-scale farmers believed that consanguineous mating-induced inbreeding has only trivial effects. High flock turnover significantly reduces inbreeding. Regardless of the low production capacity, small-scale farmers prefer local (rank = 1.47) chickens to commercial (rank = 1.61). For cocks, fertility and growth traits were highly sought after, whereas for hens, maternal instincts and laying performance were prioritized. Compared to the highlands, the lowlands had a longer egg storage period (t = 2.677, df = 117, p = 0.009, 95% CI: -3.7607, -0.5622).
CONCLUSIONS
This study documented the wisdom of small-scale farmers and encouraged the incorporation of their insights into a sustainable genetic improvement program.
Topics: Chickens; Animals; Cross-Sectional Studies; Farmers; Animal Husbandry; Breeding; Female; Male; Rural Population; Humans
PubMed: 38952253
DOI: 10.1002/vms3.1517 -
Veterinary Medicine and Science Jul 2024Annually, a massive amount of broiler litter (BL) is produced in the world, which causes soil and surface water pollution due to its high nitrogen content and microbial...
BACKGROUND
Annually, a massive amount of broiler litter (BL) is produced in the world, which causes soil and surface water pollution due to its high nitrogen content and microbial count. While ruminants can use this non-protein nitrogen (NPN) source for microbial protein synthesis. This issue becomes more critical when protein sources are unavailable or very expensive. One of the sources of NPN is BL which is produced at a considerable amount in the world yearly.
OBJECTIVES
This aim of this research was to conduct a survey of non-thermal technologies such as electrocoagulation (EC), ultraviolet (UV) radiation, and ultrasound (US) waves on the microbial safety and nutritional value of BL samples as a protein source in ruminant diets.
MATERIALS AND METHODS
The methodology of this study was based on the use of an EC device with 24 V for 60 min, UV-C light radiation (249 nm) for 1 and 10 min, and US waves with a frequency of 28 kHz for 5, 10 and 15 min to process BL samples compared with shade-dried samples. Chemical composition and nutritional values of processed samples were determined by gas production technique and measurement of fermentation parameters in vitro.
RESULTS
Based on the results, microbial safety increased in the samples processed with the US (15 min). The EC method had the best performance in reducing the number of fungi and mould. However, none of the methods could remove total bacteria and fungi. Digestibility of BL was similar in shade-dried, EC, and US (10 min) treatments. In general, the use of EC and US15 without having adverse effects on gas production caused a decrease in the concentration of ammonia nitrogen. In contrast, it caused a decrease in neutral detergent fibre (NDF) in the investigated substrate.
CONCLUSIONS
In general, it can be concluded that the use of US5 and EC methods without having a negative effect on the parameters of gas production and fermentation in vitro, while reducing NDF, causes a significant reduction in the microbial load, pathogens, yeast, and mould. Therefore, it is suggested to use these two methods to improve feed digestibility for other protein and feed sources.
Topics: Animals; Fermentation; Chickens; Nutritive Value; Ultraviolet Rays; Ultrasonic Waves; Manure
PubMed: 38952252
DOI: 10.1002/vms3.1497 -
Plant Biotechnology Journal Jul 2024
PubMed: 38952066
DOI: 10.1111/pbi.14400 -
The ISME Journal Jul 2024Microbial interactions impact the functioning of microbial communities. However, microbial interactions within host-associated communities remains poorly understood....
Microbial interactions impact the functioning of microbial communities. However, microbial interactions within host-associated communities remains poorly understood. Here, we report that the beneficiary rhizobacterium Niallia sp. RD1 requires the helper Pseudomonas putida H3 for bacterial growth and beneficial interactions with the plant host. In the absence of the helper H3 strain, the Niallia sp. RD1 strain exhibited weak respiration and elongated cell morphology without forming bacterial colonies. A transposon mutant of H3 in a gene encoding succinate-semialdehyde dehydrogenase displayed much attenuated support of RD1 colony formation. Through subsequent addition of succinate to the media, we found that succinate serves as a public good that supports RD1 growth. Comparative genome analysis highlighted that RD1 lacked the gene for sufficient succinate, suggesting its evolution as a beneficiary of succinate biosynthesis. The syntrophic interaction between RD1 and H3 efficiently protected tomato plants from bacterial wilt and promoted the tomato growth. The addition of succinate to the medium restored complex II-dependent respiration in RD1 and facilitated the cultivation of various bacterial isolates from the rhizosphere. Taken together, we delineate energy auxotrophic beneficiaries ubiquitous in the microbial community, and these beneficiaries could benefit host plants with the aid of helpers in the rhizosphere.
PubMed: 38952008
DOI: 10.1093/ismejo/wrae120 -
Genome Biology Jul 2024The massive structural variations and frequent introgression highly contribute to the genetic diversity of wheat, while the huge and complex genome of polyploid wheat...
BACKGROUND
The massive structural variations and frequent introgression highly contribute to the genetic diversity of wheat, while the huge and complex genome of polyploid wheat hinders efficient genotyping of abundant varieties towards accurate identification, management, and exploitation of germplasm resources.
RESULTS
We develop a novel workflow that identifies 1240 high-quality large copy number variation blocks (CNVb) in wheat at the pan-genome level, demonstrating that CNVb can serve as an ideal DNA fingerprinting marker for discriminating massive varieties, with the accuracy validated by PCR assay. We then construct a digitalized genotyping CNVb map across 1599 global wheat accessions. Key CNVb markers are linked with trait-associated introgressions, such as the 1RS·1BL translocation and 2NS translocation, and the beneficial alleles, such as the end-use quality allele Glu-D1d (Dx5 + Dy10) and the semi-dwarf r-e-z allele. Furthermore, we demonstrate that these tagged CNVb markers promote a stable and cost-effective strategy for evaluating wheat germplasm resources with ultra-low-coverage sequencing data, competing with SNP array for applications such as evaluating new varieties, efficient management of collections in gene banks, and describing wheat germplasm resources in a digitalized manner. We also develop a user-friendly interactive platform, WheatCNVb ( http://wheat.cau.edu.cn/WheatCNVb/ ), for exploring the CNVb profiles over ever-increasing wheat accessions, and also propose a QR-code-like representation of individual digital CNVb fingerprint. This platform also allows uploading new CNVb profiles for comparison with stored varieties.
CONCLUSIONS
The CNVb-based approach provides a low-cost and high-throughput genotyping strategy for enabling digitalized wheat germplasm management and modern breeding with precise and practical decision-making.
Topics: Triticum; DNA Copy Number Variations; Genome, Plant; High-Throughput Nucleotide Sequencing; Genetic Markers; Alleles
PubMed: 38951917
DOI: 10.1186/s13059-024-03315-6 -
Journal of Experimental & Clinical... Jul 2024Leukocyte Ig-like receptor B family 4 (LILRB4) as an immune checkpoint on myeloid cells is a potential target for tumor therapy. Extensive osteolytic bone lesion is the...
BACKGROUND
Leukocyte Ig-like receptor B family 4 (LILRB4) as an immune checkpoint on myeloid cells is a potential target for tumor therapy. Extensive osteolytic bone lesion is the most characteristic feature of multiple myeloma. It is unclear whether ectopic LILRB4 on multiple myeloma regulates bone lesion.
METHODS
The conditioned medium (CM) from LILRB4-WT and -KO cells was used to analyze the effects of LILRB4 on osteoclasts and osteoblasts. Xenograft, syngeneic and patient derived xenograft models were constructed, and micro-CT, H&E staining were used to observe the bone lesion. RNA-seq, cytokine array, qPCR, the activity of luciferase, Co-IP and western blotting were used to clarify the mechanism by which LILRB4 mediated bone damage in multiple myeloma.
RESULTS
We comprehensively analyzed the expression of LILRB4 in various tumor tissue arrays, and found that LILRB4 was highly expressed in multiple myeloma samples. The patient's imaging data showed that the higher the expression level of LILRB4, the more serious the bone lesion in patients with multiple myeloma. The conditioned medium from LILRB4-WT not -KO cells could significantly promote the differentiation and maturation of osteoclasts. Xenograft, syngeneic and patient derived xenograft models furtherly confirmed that LILRB4 could mediate bone lesion of multiple myeloma. Next, cytokine array was performed to identify the differentially expressed cytokines, and RELT was identified and regulated by LILRB4. The overexpression or exogenous RELT could regenerate the bone damage in LILRB4-KO cells in vitro and in vivo. The deletion of LILRB4, anti-LILRB4 alone or in combination with bortezomib could significantly delay the progression of bone lesion of multiple myeloma.
CONCLUSIONS
Our findings indicated that LILRB4 promoted the bone lesion by promoting the differentiation and mature of osteoclasts through secreting RELT, and blocking LILRB4 singling pathway could inhibit the bone lesion.
Topics: Multiple Myeloma; Humans; Mice; Animals; Signal Transduction; Receptors, Immunologic; NF-kappa B; Membrane Glycoproteins; Cell Line, Tumor; Osteoclasts; Xenograft Model Antitumor Assays
PubMed: 38951916
DOI: 10.1186/s13046-024-03110-y