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Chinese Herbal Medicines Oct 2021To evaluate the quality of (AR) and (DC) and study the efficacy of herbal extracts of these two herbs on the treatment of allergic contact dermatitis (ACD).
OBJECTIVE
To evaluate the quality of (AR) and (DC) and study the efficacy of herbal extracts of these two herbs on the treatment of allergic contact dermatitis (ACD).
METHODS
Qualitative and quantitative analysis of effective components was performed using High Performance Thin Layer Chromatography (HPTLC), High Performance Liquid Chromatography (HPLC), and HPLC-Quadrupole Time of Flight-Mass Spectrometry (HPLC-QTOF-MS). allergic ACD 3D model was established by incubating 3D reconstructed human epidermis (RHE) with skin sensitizer, potassium dichromate. A total of 65 gene expression that were associated with ACD, which included 24 antioxidant responsive element (ARE) and 41 SENS-IS genes were quantified by qRT-PCR. More than or equal to 10 ARE genes and 18 SENN-IS genes were induced by 1.3-fold, demonstrating the successful establishment of ACD model. Oil extracts of AR and DC were applied on the ACD model to study the efficacy.
RESULTS
Batch 3 of AR and batch 2 of DC showed presence of all active ingredients with the highest concentrations. Active ingredients of the herbs were extracted using a special oil and formulated into herbal oil extracts. The herbal oil extracts were able to down regulate the induced genes in the ACD skin model, bringing the tissue back to homeostatic status.
CONCLUSION
The oil extracts showed the potent efficacy of using AR and DC in ACD treatment. The combination study will be done to optimize the formulation ratio which will be developed into a topical cream.
PubMed: 36119360
DOI: 10.1016/j.chmed.2021.10.006 -
Ecotoxicology and Environmental Safety Oct 2022Hexavalent chromium [Cr (VI)] exists environmentally and occupationally. It has been shown to pose a carcinogenic hazard in certain occupations. This study was to...
Hexavalent chromium [Cr (VI)] exists environmentally and occupationally. It has been shown to pose a carcinogenic hazard in certain occupations. This study was to investigate the role of high mobility group A2 (HMGA2) in Cr (VI)-induced metabolism reprogramming from oxidative phosphorylation (OXPHOS) to glycolysis in A549 and HELF cells. First, knockdown of HMGA2 by siHMGA2 significantly attenuated Cr (VI)-reduced expression of OXPHOS-related proteins (COX IV and ND1) and mitochondrial mass, indicating that HMGA2 was involved in Cr (VI)-reduced OXPHOS. Overexpression of HMGA2 by transfection of HMGA2-DNA plasmids reduced the expression of COX IV, ND1 and mitochondrial mass, suggesting the negative role of HMGA2 in OXPHOS. Secondly, both CCCP, the inhibitor of mitochondrial function, and the ER stress inhibitor, 4-phenylbutyric acid (4-PBA), decreased the level of HMGA2, indicating that the interaction of mitochondrial dysfunction and ER stress resulted in Cr (VI)-induced HMGA2 expression. Further study demonstrated that ER stress/HMGA2 axis mediated the metabolism rewiring from OXPHOS to aerobic glycolysis. Notably, Cr (VI) induced the accumulation of HMGA2 proteins in mitochondria and ChIP assay demonstrated that HMGA2 proteins could bind to D-loop region of mitochondrial DNA (mtDNA), which provided the proof for HMGA2-modulating OXPHOS. Taken together, our results suggested that the interaction of mitochondria and ER stress-enhanced HMGA2 played an important role in Cr (VI)-induced metabolic reprogramming from OXPHOS to glycolysis by binding directly to D-loop region of mtDNA. This work informs on the potential mode of action for Cr (VI)-induced tumors and builds on growing evidence regarding the contribution of cellular metabolic disruption contributing to carcinogenicity.
Topics: Carbonyl Cyanide m-Chlorophenyl Hydrazone; Chromium; DNA, Mitochondrial; Glycolysis; Mitochondria
PubMed: 36116352
DOI: 10.1016/j.ecoenv.2022.114085 -
Veterinary Medicine (Auckland, N.Z.) 2022infection is one of the protozoal diseases of animals caused by various species of (intracellular parasite) and causes reduced productivity and mortality in ruminants,...
INTRODUCTION
infection is one of the protozoal diseases of animals caused by various species of (intracellular parasite) and causes reduced productivity and mortality in ruminants, especially in young ones. Despite the fact that the disease is one of the leading causes of economic losses, there is little information in Ethiopia on the occurrence of the infection in cattle and sheep.
METHODS
A cross-sectional study was conducted from December 2021 to April 2022 in and around Adama and Bishoftu towns with the objectives to estimate the prevalence of infection; identify circulating oocysts, the intensity/burden of infection and associated risk factors of infection in cattle and sheep. A total of 384 randomly selected (265 cattle and 119 sheep) fecal samples were collected from the rectum and examined by flotation technique using sheather's sugar solution to detect the oocysts of . A 2.5% potassium dichromate solution was added to the positive fecal samples for sporulation of the oocysts.
RESULTS AND DISCUSSION
The overall prevalence of 48.95% infection was recorded during the study. 45.0% and 58% prevalence of the infection was registered in cattle and sheep, respectively. There was a statistically significant difference (P ˂ 0.05) in infection between the study animal species, age of the animals, breed, farm hygiene and management system. However, there was no significant difference in infection (P > 0.05) in sex, body condition of the animals and fecal consistency. The maximum oocysts per gram of feces was found to be 10,000. infection is of great importance to livestock producers and requires serious control and prevention initiatives.
PubMed: 36110350
DOI: 10.2147/VMRR.S377017 -
RSC Advances Aug 2022Based on the gas-liquid phase chemiluminescence tester independently developed by our laboratory, a highly sensitive, fast and accurate on-line detection method of...
Based on the gas-liquid phase chemiluminescence tester independently developed by our laboratory, a highly sensitive, fast and accurate on-line detection method of formaldehyde gas in ambient atmosphere is established. The chemiluminescence system and the trace formaldehyde gas in the air directly undergo an interface heterogeneous chemiluminescence reaction to obtain a strong chemiluminescence signal. Through the measurement of the chemiluminescence signal intensity, a highly sensitive, real-time and on-line method for the determination of formaldehyde in the air was established. Factors influencing the experimental results such as gallic acid, potassium dichromate, reaction medium concentration, surfactant type and concentration, pump speed, tube length, and interfering gas were discussed based on the single factor and orthogonal analysis results. Finally, the optimal detection conditions were collected, and the detection results were compared with the national standard phenol reagent method. The results show that when the concentration of the standard formaldehyde gas is in the range of 0-0.582 μg L, the linear equation of this method is = 208 + 29.667, the linear coefficient is = 0.997, and the minimum detection concentration of formaldehyde is 2.327 × 10 μg L. Under the same external conditions, the comparison and analysis using the national standard phenol reagent method proved that this method has the advantages of fast detection speed, low detection limit, good sensitivity, and accurate results, which can be used for real-time and online determination of trace formaldehyde in ambient air.
PubMed: 36093228
DOI: 10.1039/d2ra03175b -
International Journal of Environmental... Aug 2022Efficient and sustainable technologies for cleaning of contaminated water and sediments are in urgent demand. In this study, a new type of sediment microbial fuel cell...
Efficient and sustainable technologies for cleaning of contaminated water and sediments are in urgent demand. In this study, a new type of sediment microbial fuel cell coupled floating bed (FB-SMFC) was developed to repair eutrophic water and sediment in a cleaner way. The effect of electrode spacing on the power generation capacity and the synchronous remediation of pollutants from eutrophic water and sediment were studied. When the electrode distance was 60 cm, the maximum power generation and pollutant removal effects were obtained. At the end of the experiment, the maximum output voltage was 0.4 V, and the chemical oxygen demand (COD, potassium dichromate method), total nitrogen (TN), and total phosphorus (TP) contents in the overlying water were 8 mg/L, 0.7 mg/L, and 0.39 mg/L. The corresponding removal rates were 88.2%, 78.8%, and 59.0%, respectively. The removal rates of organic matter and TN in the sediment were 12.8% and 86.4%, respectively, and the fixation rate of TP was 29.2%. Proteobacteria was the dominant phylum of bacteria in the sediment and anode. Many anaerobic bacteria were found in the overlying water, which facilitated denitrification. Overall, the results of this research revealed a highly efficient and reliable strategy for eutrophic water and sediment remediation, aquatic ecosystems restoration, and human health protection.
Topics: Bioelectric Energy Sources; Ecosystem; Electrodes; Geologic Sediments; Humans; Nitrogen; Phosphorus; Water; Water Pollutants, Chemical
PubMed: 36012057
DOI: 10.3390/ijerph191610423 -
Revista Brasileira de Parasitologia... 2022The objectives of the present study were: (1) to determine the prevalence of Eimeria spp. sporulated oocysts in calves from 26 Municipalities in the Central Zone of the...
The objectives of the present study were: (1) to determine the prevalence of Eimeria spp. sporulated oocysts in calves from 26 Municipalities in the Central Zone of the State of Veracruz, Mexico, (2) to identify the Eimeria spp. infecting calves, and (3) to identify the risk factors associated with the presence of Eimeria spp. in tropical cattle. A total of 930 individual fecal samples were analyzed by using the McMaster technique; then, oocysts were maintained in 2.5% potassium dichromate to allow sporulation. The general prevalence of calves with Eimeria spp. oocysts was 39.7% (370/930). Of a total of 10 identified species, Eimeria canadensis was the most observed, followed by Eimeria bovis and Eimeria zuernii. The statistical analysis showed an association between the age of the calves (5 to 9 months), the presence of other animals, the physiographic regions and the restricted type of husbandry with the presence of Eimeria spp. in calves (P<0.05). Protective risk factors, such as: routine coprological analysis was also associated with a decrease in infection. These data demonstrate the presence of coccidia in cattle from the State of Veracruz, additionally to the necessary measures that must be taken to control this parasitosis.
Topics: Animals; Cattle; Cattle Diseases; Coccidiosis; Eimeria; Feces; Mexico; Oocysts; Prevalence; Risk Factors
PubMed: 35946612
DOI: 10.1590/S1984-29612022043 -
Frontiers in Microbiology 2022Systemic characterization of the human gut microbiota highlighted its vast therapeutic potential. Despite having enormous potential, the non-availability of their...
Systemic characterization of the human gut microbiota highlighted its vast therapeutic potential. Despite having enormous potential, the non-availability of their culture representatives created a bottleneck to understand the concept of microbiome-based therapeutics. The present study is aimed to isolate and evaluate the probiotic potential of a human gut isolate. Physiochemical, morphological, and phylogenetic characterization of a human gut isolate identifies it as a rod-shaped gram-negative microbe taxonomically affiliated with the genus, having an optimal growth at 37°C in a partially alkaline environment (pH 8.0). This human gut isolate showed continuous growth in the presence of salts (up to 7% NaCl and 10% KCl), antibiotics, metals and metalloids [silver nitrate (up to 2 mM); lead acetate (up to 2 mM); sodium arsenate (up to 10 mM); potassium dichromate (up to 2 mM)], gastric and intestinal conditions, diverse temperature (25-50°C), and pH (5-9) conditions making it fit to survive in the highly variable gut environment. Genomic characterization identified the presence of gene clusters for diverse bio-catalytic activity, stress response, and antimicrobial activity, as well as it indicated the absence of pathogenic gene islands. A combination of functional features like anti-amylase, anti-lipase, glutenase, prolyl endopeptidase, lactase, bile salt hydrolase, cholesterol oxidase, and anti-pathogenic activity is indicative of its probiotic potential in various disorders. This was further substantiated by the CaCo-2 cell line assay confirming its cellular adherence and biosafety. Conclusively, human gut isolate possessed significant probiotic potential that can be used to promote animal and human health.
PubMed: 35910631
DOI: 10.3389/fmicb.2022.932795 -
Revista Brasileira de Parasitologia... 2022Eimeria species have importance to calves because of the economic losses. The aim of this study was to identify the species of Eimeria that affect calves and the risk...
Diversity of Eimeria (Apicomplexa: Eimeriidae) species and risk factors associated in natural infecting calves at the Southern Agreste Microregion in the State of Pernambuco, Brazil.
Eimeria species have importance to calves because of the economic losses. The aim of this study was to identify the species of Eimeria that affect calves and the risk factors associated with its natural infection. Fecal samples (387) were collected from dairy farms in the southern Agreste of Pernambuco. The feces were evaluated using the Gordon & Whitlock technique and were cultured in 2.5% potassium dichromate for sporulation of oocysts. Odds ratio (OR) were calculated to assess risk factors. Eimeria spp. were detected in 50.65% (196/387) of the samples. Eleven species were identified, being Eimeria bovis (26.64%; 548/2057), Eimeria zuernii (19.69%; 405/2057) and Eimeria ellipsoidalis (14.49%; 298/2057) those more frequent. Small herds (OR = 1.93), calves aged up to six months (OR = 2.12), absence of manure pit (OR = 7.52), fortnightly cleaning (OR = 4.71), collective calf pens (OR = 3.26), manual milking (OR = 2.16) and absence of veterinary care (OR = 2.28) were considered to be risk factors. The data revealed pathogenic species in more than 50% of the farms. Thus, the importance of adopting sanitary measures to reduce the spread of these protozoa in herds should be done, because of economic losses associated with its infection.
Topics: Animals; Brazil; Cattle; Cattle Diseases; Coccidiosis; Eimeria; Feces; Prevalence; Risk Factors
PubMed: 35613151
DOI: 10.1590/S1984-29612022026 -
Contact Dermatitis Oct 2022Allergic contact dermatitis (ACD) to Compositae is caused by sensitisation to sesquiterpene lactones (SQLs) and subsequent exposure can occur from direct handling or...
BACKGROUND
Allergic contact dermatitis (ACD) to Compositae is caused by sensitisation to sesquiterpene lactones (SQLs) and subsequent exposure can occur from direct handling or from airborne transmission. Plants from the Compositae family are ubiquitous globally and their plant extracts are also used in various products.
OBJECTIVES
Investigation of contact allergy (CA) and allergic contact dermatitis (ACD) to Compositae at a single dermatology centre.
METHODS
A retrospective case review was performed on patients undergoing patch testing to Compositae between January 2011 and December 2020 in Melbourne, Australia.
RESULTS
Of 3679 patients, 44 (1.2%) patch tested positive to Compositae and 19 (43.2%) reactions were deemed relevant. Thirteen cases (68.4%) were from direct contact with Compositae plants, mostly in gardeners. Six cases (31.6%) were from personal products and all these patients were female. Involvement of the face was significant (p = 0.007). Simultaneous allergic reactions included SQL mix in eight (42.1%), fragrance mix in seven (36.8%), potassium dichromate in three (15.8%) and colophonium in two (10.5%) cases.
CONCLUSION
Contact with Compositae from gardening contributed most cases of ACD; however, personal products accounted almost one-third of cases. Treatment options remain limited and avoidance is the most important aspect of management.
Topics: Allergens; Asteraceae; Australia; Dermatitis, Allergic Contact; Humans; Patch Tests; Plants; Retrospective Studies; Sesquiterpenes
PubMed: 35603484
DOI: 10.1111/cod.14162 -
Ecotoxicology and Environmental Safety Jul 2022Hexavalent chromium [Cr (VI)] is a common environmental pollutant. Although selenium (Se) can antagonize the toxicity of Cr (VI), the specific underlying mechanism has...
Hexavalent chromium [Cr (VI)] is a common environmental pollutant. Although selenium (Se) can antagonize the toxicity of Cr (VI), the specific underlying mechanism has not been identified. To investigate this mechanism, we used potassium dichromate (KCrO) and selenium-rich yeast (SeY) to construct single Cr (VI)- and combined Se/Cr (VI)-exposed broiler models during a 42-day period. Broilers were randomly assigned to the control (C), SeY (Se), SeY + Cr (VI) (Se/Cr), and Cr (VI) (Cr) groups. The antagonistic mechanisms of Se and Cr (VI) were evaluated using histopathological evaluation, serum and tissue biochemical tests, real-time fluorescence quantitative polymerase chain reaction, and western blotting. The results suggested that Se alleviated the morphological and structural damage to renal tubules and glomeruli, while reducing the organ index, creatinine levels, and blood urea nitrogen levels in the kidneys of Cr (VI)-exposed broilers. Furthermore, Cr (VI) reduced the levels of superoxide dismutase and glutathione, and increased the levels of malondialdehyde, in broiler kidney tissues. However, Se alleviated Cr (VI)-induced oxidative stress by increasing the levels of superoxide dismutase and glutathione, and decreasing the levels of malondialdehyde, within a certain range. Compared to the C group, the levels of p38, JNK, p-p38, p-JNK, p-p38/p38, and p-JNK/JNK significantly increased, whereas those of ERK, p-ERK, and p-ERK/ERK decreased, in the Cr group. Compared to the Cr group, the levels of p38, JNK, p-p38, p-JNK, p-p38/p38, and p-JNK/JNK significantly decreased, whereas those of ERK, p-ERK, and p-ERK/ERK increased, in the Se/Cr group. Furthermore, the levels of p53, c-Myc, Bax, Cyt-c, caspase-9, and caspase-3 significantly increased, and those of Bcl-2 and Bcl-2/Bax significantly decreased, following Cr (VI) exposure, while Se restored the expression of these genes. In conclusion, our findings suggest that SeY can protect against Cr (VI)-induced dysfunction and apoptosis by regulating the mitogen-activated protein kinase pathway activated by oxidative stress in broiler kidney tissues.
Topics: Animals; Apoptosis; Chickens; Chromium; Glutathione; Kidney; Malondialdehyde; Mitogen-Activated Protein Kinases; Selenium; Superoxide Dismutase; bcl-2-Associated X Protein
PubMed: 35576799
DOI: 10.1016/j.ecoenv.2022.113629