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Journal of Endodontics Dec 2016The aim of this study was to present a systematic review investigating the gene expression of various cells (other than dental pulp cells) in response to different... (Review)
Review
INTRODUCTION
The aim of this study was to present a systematic review investigating the gene expression of various cells (other than dental pulp cells) in response to different variants of tricalcium silicate cements (TSCs).
METHODS
A systematic search of the literature was performed by 2 independent reviewers followed by article selection and data extraction. Studies analyzing any cell type except dental pulp stem cells and any variant of tricalcium silicate cement either as the experimental or as the control group were included.
RESULTS
A total of 41 relevant articles were included in this review. Among the included studies, ProRoot MTA (Dentsply, Tulsa, OK) was the most commonly studied (69.1%) TSC variant, and 11 cell types were identified, with 13 articles investigating gene expression in osteoblasts. A total of 39 different genes/molecules expressed were found in the selected studies. The experimental group (irrespective of the TSC variant) was identified to express significantly increased gene expression compared with the control group (untreated) in all included studies. Recent studies have provided useful insight into the gene expression and molecular signaling of various cells in response to TSCs, and new elements have been supplied on the pathways activated in this process.
CONCLUSIONS
TSCs are capable of eliciting a favorable cellular response in periapical regeneration.
Topics: Calcium Compounds; Cell Line; Cell Proliferation; Cytokines; Dental Cements; Dental Cementum; Dental Materials; Dental Pulp; Fibroblasts; Gene Expression; Gene Expression Profiling; Humans; Materials Testing; Odontoblasts; Osteoblasts; Osteoclasts; Signal Transduction; Silicate Cement; Silicates; Stem Cells
PubMed: 27776883
DOI: 10.1016/j.joen.2016.08.027 -
International Endodontic Journal Jun 2016Stem cell-based therapy (SC-BT) is emerging as an alternative for endodontic therapies. The interaction between stem cells and scaffolds plays a crucial role in the... (Review)
Review
Stem cell-based therapy (SC-BT) is emerging as an alternative for endodontic therapies. The interaction between stem cells and scaffolds plays a crucial role in the generation of a 'friendly cell' microenvironment. The aim of this systematic review was to explore techniques applied to regenerate the pulp-dentine complex tissue using SC-BT. An electronic search into the SciVerse Scopus (SS), ISI Web Science (IWS) and Entrez PubMed (EP) using specific keywords was performed. Specific inclusion and exclusion criteria were predetermined. The search yielded papers, out of which full-text papers were included in the final analyses. Data extraction pooled the results in four main topics: (a) influence of the chemical properties of the scaffolds over cell behaviour; (b) influence of the physical characteristics of scaffolds over cell behaviour; (c) strategies applied to improve the stem cell/scaffold interface; and (d) influence of cue microenvironment on stem cell differentiation towards odontoblast-like cells and pulp-like tissue formation. The relationship between the scaffolds, the environment and the growth factors released from dentine are critical for de novo pulp tissue regeneration. The preconditioning of dentine walls with ethylenediaminetetraacetic acid (EDTA) was imperative for successful pulp-dentine complex regeneration. An analyses of the grouped results revealed that pulp regeneration was an attainable goal.
Topics: Dental Pulp; Guided Tissue Regeneration; Humans; Stem Cell Transplantation; Tissue Engineering; Tissue Scaffolds
PubMed: 26101143
DOI: 10.1111/iej.12489 -
Dental Materials : Official Publication... Aug 2010To quantitatively determine whether the pulp response to resin-modified glass ionomer cements placed in deep cavities differs from that generated by calcium hydroxide... (Comparative Study)
Comparative Study Meta-Analysis Review
OBJECTIVE
To quantitatively determine whether the pulp response to resin-modified glass ionomer cements placed in deep cavities differs from that generated by calcium hydroxide cement.
SOURCES
Five databases were searched for articles up to 31 May 2009.
INCLUSION CRITERIA
titles/abstracts relevant to topic; published in English; two-arm longitudinal in vivo trial; containing computable dichotomous datasets for test and control group.
EXCLUSION CRITERIA
not all entered subjects accounted for at the end of the trial; subjects of both groups not followed up in the same way; trial on animal tissue.
DATA
One randomized and five non-randomized control trials, reporting on 1 and 17 datasets, respectively, were accepted. From non-randomized trials, the Relative Risk with 95% Confidence Interval of 13 datasets showed no statistically significant differences (p>0.05) and 4 showed a statistically significant difference between both materials. Meta-analysis of datasets from these trials found no difference between the inflammatory cell response after 30 days (0.87; 95%CI 0.59-1.26; p=0.46); 38% less inflammatory cell response with calcium hydroxide after 60 days (0.62; 95%CI 0.50-0.76; p<0.00001); higher number of intact odontoblasts beneath restored cavities after 381 days (0.56; 95%CI 0.38-0.82; p=0.0008). The results from the randomized control trial (1.40; 95%CI 0.92-2.14; p=0.11) indicated no difference in clinically identifiable pulp symptoms after two years. All trials showed limited internal validity due to selection bias.
CONCLUSION
No conclusive statement about the superiority of either type of material can yet be made. Further high-quality randomized control trials are needed.
Topics: Animals; Calcium Hydroxide; Chi-Square Distribution; Controlled Clinical Trials as Topic; Dental Caries; Dental Cavity Lining; Dental Pulp; Dental Pulp Capping; Glass Ionomer Cements; Humans; Odds Ratio; Odontoblasts
PubMed: 20452013
DOI: 10.1016/j.dental.2010.03.021