-
Applied and Environmental Microbiology Jun 1992Some protozoans isolated from aquatic habitats, including domestic water supplies, can support the intracellular replication of autochthonous legionellae in vitro. We...
Some protozoans isolated from aquatic habitats, including domestic water supplies, can support the intracellular replication of autochthonous legionellae in vitro. We studied the effect of incubating water samples containing amoebae on the sensitivity of culture for legionellae. Samples collected during investigations of legionellosis epidemics and shown by conventional culture procedures to contain amoebae, but not legionellae, were incubated at 35 degrees C and replated. Legionellae were recovered from 59 of 144 such samples. Species isolated included L. pneumophila, L. anisa, L. bozemanii, L. gormanii, L. micdadei, L. rubrilucens, L. sainthelensi, L. steigerwaltii, and an unnamed species. Acanthamoeba polyphaga, Acanthamoeba hatchetti, a Rosculus sp., Hartmannella vermiformis, and Vahlkampfia spp. were among the autochthonous amoebae identified. Legionellae were recovered by this procedure from only 3 of 63 samples that were negative for amoebae by conventional culture procedures. These results show that water samples negative for legionellae, but positive for amoebae, by standard culture techniques should be incubated and replated to maximize the sensitivity of culture for legionellae.
Topics: Amoeba; Animals; Bacteriological Techniques; Cell Division; Ecology; Legionella; Sensitivity and Specificity; Species Specificity; Water Microbiology
PubMed: 1622278
DOI: 10.1128/aem.58.6.2001-2004.1992 -
Comptes Rendus de L'Academie Des... 1989The intracorneal inoculation of nude mice with Acanthamoeba hatchetti amoebae induced a keratitis with disruption of corneal fibres, inflammatory response, secondary...
The intracorneal inoculation of nude mice with Acanthamoeba hatchetti amoebae induced a keratitis with disruption of corneal fibres, inflammatory response, secondary vascularization and amoebic trophozoites.
Topics: Acanthamoeba; Acanthamoeba Keratitis; Animals; Cornea; Disease Models, Animal; Eye; Female; Mice; Mice, Nude; Neutrophils
PubMed: 2516476
DOI: No ID Found -
Bio Systems 1985Isoenzyme electrophoresis of three different enzyme systems was used to compare 71 strains assigned to the 15 currently recognized species of Acanthamoeba. A... (Comparative Study)
Comparative Study
Isoenzyme electrophoresis of three different enzyme systems was used to compare 71 strains assigned to the 15 currently recognized species of Acanthamoeba. A phylogenetic (cladistic) analysis of the zymograms indicated an arrangement of strains in 15 distinguishable lineages, but not all corresponding to current taxonomic assignments. Five of the groups corresponded to the recognized species A. castellanii, A. culbertsoni, A. griffini, A. lenticulata and A. royreba. But none of these groups consisted of only strains which had been previously assigned to each respective species. The type-equivalent strains for two species, A. hatchetti and A. tubiashi, were not closely aligned to any other strain and thus are considered to be monotypic. Strains of A. triangularis, A. astronyxis and A. palestinensis occurred together in a single group suggesting possible synonymy; however, on morphologic criteria, the strains assigned to these species are readily distinguishable. Strains assigned to A. polyphaga and A. rhysodes were interspersed throughout the other species groups. The strains of these two species were either misidentified or the species could not be recognized. Two groups previously not recognized as unique formed distinctive clusters which could be considered as new species. The analysis also made it possible to place strains which had previously been identified only to genus into species complexes. These results therefore suggest that previous criteria which have been used to classify Acanthamoeba are not adequate for fully resolving taxa at the species level.
Topics: Amoeba; Animals; Electrophoresis; Isoenzymes; Phylogeny; Species Specificity
PubMed: 4084681
DOI: 10.1016/0303-2647(85)90039-5 -
Medecine Tropicale : Revue Du Corps de... 1982We tried to prove the part of the little savage mammalians as active intermediate hosts to the intestine level for some free-living limax amoebae of limax group. For...
We tried to prove the part of the little savage mammalians as active intermediate hosts to the intestine level for some free-living limax amoebae of limax group. For this we participated to several trapping-season in Britanny and in Tunisia. From 273 samplings, we studied 224 with the results thereafter: the rodents are the first (213/224) and the 3 most frequent species are also the most often hosts: 20/128 A. sylvaticus, 9/58 C. glareolus and 4/16 M. spretus. There must be a double positive factor: life in burrow near the earth and meal type. The 78 samplings for which we checked the free-living amoebae by stool examination, are the most poor by culture: 2/78 (2.5 p. 100). This lack explains the negative stool examination. The isolated amoebae belong for 31/34 to the genus Acanthamoeba, of one potentially pathogenic: A. hatchetti. Moreover the Finistere biotop appears particularly poor (2.5 p. 100 carriers in front of 21 p. 100 in Ille-et-Vilaine and 24 p. 100 in Tunisia).
Topics: Amoeba; Animal Population Groups; Animals; Animals, Wild; Disease Vectors; Feces; France; Rodentia; Tunisia
PubMed: 7109906
DOI: No ID Found -
Annales de Parasitologie Humaine Et... 1979From march 1976 until december 1978, we have analysed 1039 nasal swabs in order to discover the healthy free-living amoebae carriers. So, we have isolated 9 strains of...
From march 1976 until december 1978, we have analysed 1039 nasal swabs in order to discover the healthy free-living amoebae carriers. So, we have isolated 9 strains of which one Hartmannella vermiformis. From the 8 remaining Acanthamoebae, only one, ORL 561 (Acanthamoeba hatchetti) is as pathogenic for mice as the 2 other identical known strains and A. culbertsoni.
Topics: Adolescent; Adult; Amebiasis; Amoeba; Animals; Carrier State; Child; Culture Techniques; Female; Haplorhini; Humans; Kidney; Male; Mice; Middle Aged; Nasal Mucosa; Occupations; Risk; Species Specificity
PubMed: 120123
DOI: No ID Found -
Science (New York, N.Y.) Jun 1977Acanthamoeba culbertsoni was isolated from a sewage-spoil dump site near Ambrose Light, New York Bight. A second species, Acanthamoeba hatchetti, n. sp., was isolated...
Acanthamoeba culbertsoni was isolated from a sewage-spoil dump site near Ambrose Light, New York Bight. A second species, Acanthamoeba hatchetti, n. sp., was isolated from Brewerton Channel, Baltimore Harbor, Maryland. Both species killed laboratory mice after infection by the intranasal route.
Topics: Amebiasis; Amoeba; Animals; Body Weight; Brain; Lung; Mice; Refuse Disposal; Seawater; Soil Microbiology; Water Microbiology
PubMed: 867031
DOI: 10.1126/science.867031