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Journal of Chromatography. B,... Jan 2000A chemotaxonomic study of some corynebacteria isolated from clinical samples revealed characteristic thin-layer chromatographic patterns for meso-diaminopimelic acid...
A chemotaxonomic study of some corynebacteria isolated from clinical samples revealed characteristic thin-layer chromatographic patterns for meso-diaminopimelic acid containing species included in the genera Corynebacterium, Dermabacter and Brevibacterium. Notably, a specific compound was consistently detected in mycolic acid containing species of the genus Corynebacterium. This compound was composed by glycerol and mycolic acids and structural analyses carried out by fast atom bombardment mass spectrometry in C. minutissimum confirmed its identification as mycoloylglycerol. The chain length of mycoloyl groups in this molecule ranged from 28 to 34 carbon atoms, being mono-, di- or triunsaturated. Detection of mycoloylglycerol by thin-layer chromatography may be thus useful for the rapid inclusion of a great variety of corynebacteria of clinical origin in the genus Corynebacterium in laboratories employing chromatographic techniques as an adjunct for the identification of these microorganisms.
Topics: Brevibacterium; Chromatography, Thin Layer; Corynebacterium; Glycerol; Mycolic Acids
PubMed: 10778941
DOI: 10.1016/s0378-4347(99)00495-8 -
Infection 1998Dermabacter species are commonly isolated from the skin and other body sites but rarely show pathogenicity in humans. A case of Dermabacter hominis cerebral abscess is... (Review)
Review
Dermabacter species are commonly isolated from the skin and other body sites but rarely show pathogenicity in humans. A case of Dermabacter hominis cerebral abscess is reported which presented as a contrast-enhancing intracranial mass in a renal transplant patient.
Topics: Adult; Brain Abscess; Diagnosis, Differential; Fatal Outcome; Female; Gram-Positive Bacterial Infections; Humans; Magnetic Resonance Imaging
PubMed: 9646113
DOI: 10.1007/BF02771848 -
Clinical Microbiology Reviews Jan 1997Coryneform bacteria are aerobically growing, asporogenous, non-partially-acid-fast, gram-positive rods of irregular morphology. Within the last few years, there has been... (Review)
Review
Coryneform bacteria are aerobically growing, asporogenous, non-partially-acid-fast, gram-positive rods of irregular morphology. Within the last few years, there has been a massive increase in the number of publications related to all aspects of their clinical microbiology. Clinical microbiologists are often confronted with making identifications within this heterogeneous group as well as with considerations of the clinical significance of such isolates. This review provides comprehensive information on the identification of coryneform bacteria and outlines recent changes in taxonomy. The following genera are covered: Corynebacterium, Turicella, Arthrobacter, Brevibacterium, Dermabacter. Propionibacterium, Rothia, Exiguobacterium, Oerskovia, Cellulomonas, Sanguibacter, Microbacterium, Aureobacterium, "Corynebacterium aquaticum," Arcanobacterium, and Actinomyces. Case reports claiming disease associations of coryneform bacteria are critically reviewed. Minimal microbiological requirements for publications on disease associations of coryneform bacteria are proposed.
Topics: Aerobiosis; Bacteriological Techniques; Centers for Disease Control and Prevention, U.S.; Corynebacterium; Corynebacterium Infections; Humans; Microbial Sensitivity Tests; United States
PubMed: 8993861
DOI: 10.1128/CMR.10.1.125 -
Antimicrobial Agents and Chemotherapy Dec 1996The susceptibility patterns of 480 isolates representing six recently defined species of coryneform bacteria (Corynebacterium amycolatum [n = 101], Corynebacterium auris...
The susceptibility patterns of 480 isolates representing six recently defined species of coryneform bacteria (Corynebacterium amycolatum [n = 101], Corynebacterium auris [n = 48], Corynebacterium glucuronolyticum [n = 86], Brevibacterium casei [n = 50], Dermabacter hominis [n = 49], and Turicella otitidis [n = 146]) to 17 antimicrobial agents were determined by an agar dilution method. Most significantly, for C. amycolatum strains the MICs at which 90% of isolates are inhibited were > or = 32 micrograms/ml for nearly all agents. However, all 480 strains examined were susceptible to glycopeptide antibiotics.
Topics: Actinomycetales; Anti-Bacterial Agents; Drug Resistance, Microbial; Glycopeptides; Microbial Sensitivity Tests
PubMed: 9124857
DOI: 10.1128/AAC.40.12.2874 -
Zentralblatt Fur Bakteriologie :... Jul 1996An identification scheme for aerobically growing Gram-positive rods (genera Actinomyces, Arcanobacterium, Aureobacterium, Bacillus, Brevibacterium, Cellulomonas,...
An identification scheme for aerobically growing Gram-positive rods (genera Actinomyces, Arcanobacterium, Aureobacterium, Bacillus, Brevibacterium, Cellulomonas, Corynebacterium, Dermabacter, Erysipelothrix, Gardnerella, Lactobacillus, Listeria, Microbacterium, Oerskovia, Propionibacterium, Rhodococcus, Rothia, Turicella, as well as unnamed CDC groups, Clostridium tertium, and Mycobacterium fortuitum/chelonae) is presented. It is derived from the Hollis-Weaver scheme and uses catalase, oxidative/fermentative carbohydrate metabolism and motility as primary reactions. Tests for lipophilism, nitrate reduction, urease, esculin hydrolysis, the CAMP reaction, acid formation from five carbohydrates, as well as for some facultative reactions should lead to a correct diagnosis based on information available at the end of 1995.
Topics: Aerobiosis; Carbohydrates; Catalase; Gram-Positive Rods
PubMed: 8837385
DOI: 10.1016/s0934-8840(96)80100-9 -
Journal of Clinical Microbiology Aug 1994Thirty strains of fermentative coryneform-like bacteria designated CDC fermentative coryneform group 3 and coryneform group 5 were compared biochemically by cellular...
Thirty strains of fermentative coryneform-like bacteria designated CDC fermentative coryneform group 3 and coryneform group 5 were compared biochemically by cellular fatty acid analysis and by DNA relatedness with the type strain of Dermabacter hominis, ATCC 49369. DNA from 22 strains of both CDC groups showed 69 to 96% relatedness (hydroxyapatite method) to labeled DNA from ATCC 49369 and to DNA from CDC group 3 strain G4964, and the strains are considered to belong to D. hominis. The remaining eight strains were genetically but not phenotypically differentiable from D. hominis. They were genetically heterogeneous, but hybridization results indicated that they probably belong to the genus Dermabacter. Thirteen of the 22 D. hominis strains and all 8 of the other Dermabacter strains had been isolated from blood, which indicates the pathogenic potential of this species and genus.
Topics: Actinomycetales; Actinomycetales Infections; Adult; Aged; Bacterial Typing Techniques; Centers for Disease Control and Prevention, U.S.; DNA, Bacterial; Fatty Acids; Female; Humans; Infant; Infant, Newborn; Male; Middle Aged; Nucleic Acid Hybridization; United States
PubMed: 7989543
DOI: 10.1128/jcm.32.8.1918-1922.1994 -
International Journal of Systematic... Jul 199416S rRNA gene sequencing studies were performed on Dermabacter hominis and four meso-diaminopimelic acid-containing species of the genus Brevibacterium. Phylogenetic...
16S rRNA gene sequencing studies were performed on Dermabacter hominis and four meso-diaminopimelic acid-containing species of the genus Brevibacterium. Phylogenetic analysis revealed a close association between Dermabacter hominis and representatives of the lysine-containing genera Arthrobacter, Micrococcus, and Renibacterium. By contrast, the genus Brevibacterium formed a distinct line of descent within the high-guanine-plus-cytosine-containing actinomycetes, displaying no specific affinity with any other organism examined.
Topics: Actinomycetales; Base Composition; Brevibacterium; Diaminopimelic Acid; Molecular Sequence Data; Phylogeny; RNA, Bacterial; RNA, Ribosomal, 16S; Species Specificity
PubMed: 7520745
DOI: 10.1099/00207713-44-3-583 -
Journal of Clinical Microbiology May 1994Over a 1-year period, 11 isolates (including 5 from blood cultures) of the recently described CDC group 3 and group 5 coryneform bacteria were derived from clinical...
Over a 1-year period, 11 isolates (including 5 from blood cultures) of the recently described CDC group 3 and group 5 coryneform bacteria were derived from clinical specimens and compared with reference strains. Biochemical characteristics indicated a very close relationship between CDC group 3 and group 5 coryneform bacteria. The ability of CDC group 3 and the inability of CDC group 5 coryneform bacteria to ferment xylose were the only reactions that were different for the two taxa. Chemotaxonomic features of the two groups included the presence of meso-diaminopimelic acid, a lack of mycolic acids, and the presence of predominantly branched cellular fatty acids, a combination found among gram-positive rods only in Brevibacterium spp., Brachybacterium faecium, and Dermabacter hominis. 16S rRNA gene sequence analysis revealed that CDC group 3 and group 5 coryneform bacteria are members of the genus Dermabacter, which to date has been isolated exclusively from human skin.
Topics: Actinomycetales; Actinomycetales Infections; Bacteremia; Bacterial Typing Techniques; Base Sequence; DNA, Bacterial; DNA, Ribosomal; Fermentation; Humans; Molecular Sequence Data; Species Specificity; Xylose
PubMed: 8051248
DOI: 10.1128/jcm.32.5.1223-1228.1994