Did you mean: diphtheroid bacillus
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Journal of Infection Prevention Jan 2019The objectives of our study were to study the stethoscope cleaning practices of medical personnel and to assess the microbial load on stethoscopes and efficacy of...
BACKGROUND
The objectives of our study were to study the stethoscope cleaning practices of medical personnel and to assess the microbial load on stethoscopes and efficacy of cleaning stethoscopes with alcohol-based disinfectant.
METHODOLOGY
A questionnaire-based cross-sectional study was conducted among medical personnel at a tertiary care hospital in India to assess their knowledge and cleaning practices regarding stethoscope disinfection. Samples from the stethoscopes were collected before and after cleaning with alcohol-based disinfectant.
RESULTS
Out of 62 participants, 53.22% individuals had never cleaned their stethoscope. All the initial swab samples showed bacterial growth. There was mixed growth in the samples taken from 35 stethoscope bells and 31 diaphragms. Bacteria included coagulase negative staphylococci, bacillus species, diptheroids, and After cleaning with an alcohol-based disinfectant, there was a significant decrease in the average number of bacterial colony-forming units.
CONCLUSIONS
Regular cleaning practices should be followed to prevent growth and transmission of potentially pathogenic organisms.
PubMed: 30719088
DOI: 10.1177/1757177418802353 -
Surgical Infections Oct 2014Clostridum difficile is a gram-positive, spore-forming anaerobic bacillus that has substantial associated morbidity, mortality, and associated healthcare burdens....
BACKGROUND
Clostridum difficile is a gram-positive, spore-forming anaerobic bacillus that has substantial associated morbidity, mortality, and associated healthcare burdens. Clostridium difficile spores are not destroyed by alcohol. Alcohol gel dispensers are used commonly as the hand sanitization method of choice in hospitals. It is possible that gel dispensers are fomites for C. difficile.
METHODS
Thirty alcohol-based gel dispenser handles outside of rooms of patients with active C. difficile infection were sampled. The samples were assessed for C. difficile by both culture and polymerase chain reaction (PCR). The samples were also assessed for other organisms by culture.
RESULTS
No C. difficile was cultured or detected by PCR on any of the gel dispensers. Coagulase-negative Staphyloccus spp., diptheroids, and Bacillus spp. were the organisms detected most commonly.
CONCLUSION
At our institution, C. difficile is not present on alcohol-based gel dispensers, but other potentially pathogenis are.
Topics: Academic Medical Centers; Bacteria; Clostridioides difficile; Enterocolitis, Pseudomembranous; Environmental Microbiology; Fomites; Hand Disinfection; Hand Sanitizers; Humans; Polymerase Chain Reaction
PubMed: 25126976
DOI: 10.1089/sur.2013.102 -
Journal of Veterinary Internal Medicine 1998Quantitative urine cultures were performed on 11 male and 11 female healthy adult horses. Urine was collected by free catch and catheterization using standard methods....
Quantitative urine cultures were performed on 11 male and 11 female healthy adult horses. Urine was collected by free catch and catheterization using standard methods. Results showed that all samples collected by free catch contained less than 20,000 colony-forming units (CFU)/mL. All samples collected by catheterization contained 500 CFU/mL or less. A significant difference was found between collection methods (P < .005), with catheterization having less contamination. In samples collected by free catch, females had significantly greater contamination than did males (P < .03). Predominant bacterial species isolated included Streptococcus spp., Escherichia coli, Enterobacter sp., Bacillus sp., Staphylococcus spp., Diptheroids sp., Proteus spp., and Enterococcus sp. Many samples contained multiple bacterial species. Bacterial isolates were representative of the normal bacterial flora of the equine urogenital tract. This paper establishes reference values for quantitative urine culture results in healthy adult horses to aid in the diagnosis of urinary tract infections.
Topics: Animals; Bacteria; Electrolytes; Female; Horses; Male; Reference Values; Specimen Handling; Urinalysis; Urination; Urine
PubMed: 9560762
DOI: 10.1111/j.1939-1676.1998.tb02098.x -
Canadian Journal of Microbiology Oct 1977Micrococcus luteus produced a diffusible, esterase inhibitory factor (EIF) which inhibited the activity of cutaneous diphtheroid esterases on Tween 80-CaCl2 agar media....
Micrococcus luteus produced a diffusible, esterase inhibitory factor (EIF) which inhibited the activity of cutaneous diphtheroid esterases on Tween 80-CaCl2 agar media. Esterases of Staphylococcus, Micrococcus, Bacillus, and Serratia were not susceptible. EIF did not appear to combine with the substrate or to prevent enzyme synthesis; it was unable to reverse the precipitation of calcium oleate. The composition of the medium, especially peptones, influenced the production of EIF. EIF was synthesized in the absence of diphtheroids, but production required the presence of Tween. The interaction was observed on agar medium of pH 5.5-8.5, at 25-43 degrees C, under an atmosphere of 10-20% CO2, in the presence of urea, but not after the addition of NaCl or dextrose. Supernatants of broth cultures had to be concentrated to detect EIF. Crude dialyzed and concentrated preparations of EIF withstood 60 degrees C for 60 min but were inactivated after 100 degrees C for 10 min. EIF may possibly be associated with a lipoid substance, since it did not precipitate in ethanol.
Topics: Bacillus; Corynebacterium diphtheriae; Esterases; Hot Temperature; Lipase; Micrococcus; Serratia; Sorbitol; Species Specificity; Staphylococcus
PubMed: 411559
DOI: 10.1139/m77-200