-
BMC Microbiology Jul 2012Bacterial infections have been linked to malignancies due to their ability to induce chronic inflammation. We investigated the association of oral bacteria in oral... (Comparative Study)
Comparative Study
BACKGROUND
Bacterial infections have been linked to malignancies due to their ability to induce chronic inflammation. We investigated the association of oral bacteria in oral squamous cell carcinoma (OSCC/tumor) tissues and compared with adjacent non-tumor mucosa sampled 5 cm distant from the same patient (n = 10). By using culture-independent 16S rRNA approaches, denaturing gradient gel electrophoresis (DGGE) and cloning and sequencing, we assessed the total bacterial diversity in these clinical samples.
RESULTS
DGGE fingerprints showed variations in the band intensity profiles within non-tumor and tumor tissues of the same patient and among the two groups. The clonal analysis indicated that from a total of 1200 sequences characterized, 80 bacterial species/phylotypes were detected representing six phyla, Firmicutes, Bacteroidetes, Proteobacteria, Fusobacteria, Actinobacteria and uncultivated TM7 in non-tumor and tumor libraries. In combined library, 12 classes, 16 order, 26 families and 40 genera were observed. Bacterial species, Streptococcus sp. oral taxon 058, Peptostreptococcus stomatis, Streptococcus salivarius, Streptococcus gordonii, Gemella haemolysans, Gemella morbillorum, Johnsonella ignava and Streptococcus parasanguinis I were highly associated with tumor site where as Granulicatella adiacens was prevalent at non-tumor site. Streptococcus intermedius was present in 70% of both non-tumor and tumor sites.
CONCLUSIONS
The underlying changes in the bacterial diversity in the oral mucosal tissues from non-tumor and tumor sites of OSCC subjects indicated a shift in bacterial colonization. These most prevalent or unique bacterial species/phylotypes present in tumor tissues may be associated with OSCC and needs to be further investigated with a larger sample size.
Topics: Bacteria; Biodiversity; Carcinoma, Squamous Cell; Cloning, Molecular; Denaturing Gradient Gel Electrophoresis; Female; Humans; Male; Metagenome; Middle Aged; Mouth Mucosa; Mouth Neoplasms; RNA, Ribosomal, 16S; Sequence Analysis, DNA
PubMed: 22817758
DOI: 10.1186/1471-2180-12-144 -
International Journal of Systematic... Oct 1995Comparative 16S rRNA analysis was used to determine the phylogenetic positions of Catonella morbi and Johnsonella ignava, which are members of two monospecific genera of...
Evidence for the placement of the gram-negative Catonella morbi (Moore and Moore) and Johnsonella ignava (Moore and Moore) within the Clostridium subphylum of the gram-positive bacteria on the basis of 16S rRNA sequences.
Comparative 16S rRNA analysis was used to determine the phylogenetic positions of Catonella morbi and Johnsonella ignava, which are members of two monospecific genera of gram-negative anaerobic bacilli isolated from human gingival crevices. Both of these genera were found to belong to cluster XIVa (M. D. Collins, P. A. Lawson, A. Willems, J. J. Cordoba, J. Fernandez-Garayzabal, P. Garcia, J. Cai, H. Hippe, and J. A. E. Farrow, Int. J. Syst. Bacteriol. 44:812-826, 1994) of the Clostridium subphylum of gram-positive bacteria. Within this cluster, which contains several Clostridium, Coprococcus, Eubacterium, and Ruminococcus species, C. morbi and J. ignava formed two distinct lines that were separate from all other taxa. Our findings support the separate generic status of the genera Catonella and Johnsonella and show that these genera do not belong to the family Bacteroidaceae but instead belong to the gram-positive Clostridium subphylum.
Topics: Base Sequence; Clostridium; Gram-Negative Anaerobic Bacteria; Humans; Molecular Sequence Data; RNA, Bacterial; RNA, Ribosomal, 16S
PubMed: 7547310
DOI: 10.1099/00207713-45-4-855 -
International Journal of Systematic... Apr 1994The following four new species of anaerobic gram-negative bacilli isolated from the human gingival crevice are described: Oribaculum catoniae, with ATCC 51270 as the... (Comparative Study)
Comparative Study
Oribaculum catoniae gen. nov., sp. nov.; Catonella morbi gen. nov., sp. nov.; Hallella seregens gen. nov., sp. nov.; Johnsonella ignava gen. nov., sp. nov.; and Dialister pneumosintes gen. nov., comb. nov., nom. rev., Anaerobic gram-negative bacilli from the human gingival crevice.
The following four new species of anaerobic gram-negative bacilli isolated from the human gingival crevice are described: Oribaculum catoniae, with ATCC 51270 as the type strain; Catonella morbi, with ATCC 51271 as the type strain; Hallella seregens, with ATCC 51272 as the type strain; and Johnsonella ignava, with ATCC 51276 as the type strain. C. morbi is associated with periodontitis. H. seregens and J. ignava are associated with gingivitis and periodontitis. O. catoniae has been isolated from healthy and diseased gingiva. Dialister pneumosintes (Olitsky and Gates 1921) gen. nov., comb. nov., nom. rev., associated with gingivitis, is proposed to accommodate organisms formerly classified as Bacteroides pneumosintes.
Topics: Bacterial Typing Techniques; Fatty Acids; Gingival Pocket; Gingivitis; Gram-Negative Anaerobic Bacteria; Gram-Negative Bacterial Infections; Humans; Periodontitis
PubMed: 8186083
DOI: 10.1099/00207713-44-2-187