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Analytical Sciences : the International... Jul 2024The level of interleukin-8 (IL-8) in the body is an effective factor for the early diagnosis of acute tubular necrosis and oral tumor. In this work, a novel...
The level of interleukin-8 (IL-8) in the body is an effective factor for the early diagnosis of acute tubular necrosis and oral tumor. In this work, a novel sandwich-like voltametric immunosensor (SVS) of IL-8 was constructed by preparing β-cyclodextrin/carbon nanotube (CD/CNT) to immobilize primary antibody (PAb) of IL-8 and UIO-66-NH MOFs structure to immobilize second antibody (SAb) and methylene blue (Mb) probe. In this designed SVS, the prepared CD/CNT nanohybrid with large surface area and conductivity can immobilize PAb via simple host-guest recognition, and UIO-66-NH provided an ideal platform to accommodate SAb and a large number of Mb molecules as signal-amplifier. In the existence of target IL-8, the current peak of Mb from the SVS assay increases with the increasement of IL-8 level. Through optimizing and adjusting various factors, a wide linearity (0.001-2.5 ng mL) and low analytical limit (0.2 pg mL) of IL-8 were realized, so it's expected the developed SVS strategy has significant applications for the detection of IL-8.
Topics: Nanotubes, Carbon; Interleukin-8; beta-Cyclodextrins; Immunoassay; Biosensing Techniques; Humans; Metal-Organic Frameworks; Limit of Detection; Antibodies, Immobilized
PubMed: 38662337
DOI: 10.1007/s44211-024-00574-y -
Chemistry (Weinheim An Der Bergstrasse,... Jun 2024Radical-polar crossover reactions were studied for the intramolecular cyclopropanation of active methylene derivatives. In the presence of FeCl as a stoichiometric...
Radical-polar crossover reactions were studied for the intramolecular cyclopropanation of active methylene derivatives. In the presence of FeCl as a stoichiometric oxidant and KHPO as a base, the dehydrogenative cyclopropanation of active methylenes proceeded through the FeCl-promoted oxidative radical cyclization followed by the ionic cyclization to give the bicyclic cyclopropanes. The use of α-chloro-active methylenes leads the subcatalytic cyclopropanation involving two redox pathways. In the presence of KHPO, the redox cyclopropanation proceeded by using FeCl (20 mol%) in combination with ligand (20 mol%).
PubMed: 38658317
DOI: 10.1002/chem.202400602 -
ACS Applied Materials & Interfaces Apr 2024Cellulose nanofiber (CNF) holds great promise in applications such as surface-enhanced Raman scattering (SERS), catalysis, esthesia, and detection. This study aimed to...
Cellulose nanofiber (CNF) holds great promise in applications such as surface-enhanced Raman scattering (SERS), catalysis, esthesia, and detection. This study aimed to build novel CNF-based SERS substrates through a facile synthetic method. Citrate-reduced gold nanoparticles (AuNPs) were adsorbed on the cationized CNF surface due to electrostatic interactions, and uniform AuNPs@(2,3-epoxypropyl trimethylammonium chloride)EPTMAC@CNF flexible SERS substrates were prepared by a simple vacuum-assisted filtration method. The probe molecule methylene blue was chosen to assess the performance of the CNF-based SERS substrate with a sensitivity up to 10 M, superior signal reproducibility (relative standard deviation (RSD) = 4.67%), and storage stability (more than 30 days). Tensile strength tests indicated that the CNF-based films had good mechanical properties. In addition, CNF-based substrates can easily capture and visually identify microplastics in water. These results demonstrate the potential application of the flexible, self-assembled AuNPs@EPTMAC@CNF flexible SERS substrate for prompt and sensitive detection of trace substances.
PubMed: 38657211
DOI: 10.1021/acsami.4c03255 -
Heliyon Apr 2024Methylene blue (MB) was found to exert neuroprotective effect on different brain diseases, such as ischemic stroke. This study assessed the MB effects on ischemia...
The protective effects of methylene blue on astrocytic swelling after cerebral ischemia-reperfusion injuries are mediated by Aquaporin-4 and metabotropic glutamate receptor 5 activation.
Methylene blue (MB) was found to exert neuroprotective effect on different brain diseases, such as ischemic stroke. This study assessed the MB effects on ischemia induced brain edema and its role in the inhibition of aquaporin 4 (AQP4) and metabotropic glutamate receptor 5 (mGluR5) expression. Rats were exposed 1 h transient middle cerebral artery occlusion (tMCAO), and MB was injected intravenously following reperfusion (3 mg/kg). Magnetic resonance imaging (MRI) and 2,3,5-triphenyltetrazolium chloride (TTC) staining was performed 48 h after the onset of tMCAO to evaluate the brain infarction and edema. Brain tissues injuries as well as the glial fibrillary acidic protein (GFAP), AQP4 and mGluR5 expressions were detected. Oxygen and glucose deprivation/reoxygenation (OGD/R) was performed on primary astrocytes (ASTs) to induce cell swelling. MB was administered at the beginning of reoxygenation, and the perimeter of ASTs was measured by GFAP immunofluorescent staining. 3,5-dihydroxyphenylglycine (DHPG) and fenobam were given at 24 h before OGD to examine their effects on MB functions on AST swelling and AQP4 expression. MB remarkably decreased the volumes of T2WI and ADC lesions, as well as the cerebral swelling. Consistently, MB treatment significantly decreased GFAP, mGluR5 and AQP4 expression at 48 h after stroke. In the cultivated primary ASTs, OGD/R and DHPG significantly increased ASTs volume as well as AQP4 expression, which was reversed by MB and fenobam treatment. The obtained results highlight that MB decreases the post-ischemic brain swelling by regulating the activation of AQP4 and mGluR5, suggesting potential applications of MB on clinical ischemic stroke treatment.
PubMed: 38644842
DOI: 10.1016/j.heliyon.2024.e29483 -
Journal of Experimental Zoology. Part... Jul 2024Type 2 diabetes (T2D) is the most common metabolic disorder. The undesirable effects of synthetic drugs demand a search for safe antidiabetic agents. This study aimed to...
Type 2 diabetes (T2D) is the most common metabolic disorder. The undesirable effects of synthetic drugs demand a search for safe antidiabetic agents. This study aimed to assess the antidiabetic activity of different fractions of Atriplex halimus (petroleum ether 60-80, methylene chloride, ethyl acetate, and n-butanol) using Drosophila melanogaster larvae. Titers of total glucose and trehalose, as well as larval weight, were measured and compared with those of control and diabetic larvae. The expression of Drosophila insulin-like peptides (DILP2 and DILP3) and adipokinetic hormone (AKH) was evaluated. The results revealed a significant increase in total glucose, trehalose, and a decrease in body weight in the larvae fed a high-sugar diet compared with those in the control. When larvae fed diets containing the tested fractions, the total glucose and trehalose decreased to the control level, and the body weight increased. DILP2, DILP3, and AKH exhibited significant decreases upon treatment with A. halimus ethyl acetate. Metabolomic profiling of the ethyl acetate fraction of A. halimus revealed the presence of flavonoids and flavonoid glycosides. After docking screening to predict the most powerful moiety, we discovered that flavonoid glycosides (especially eriodictyol-7-O-neohesperidoside) have a greater affinity for the pocket than the other moieties. The results indicated the therapeutic activity of the A. halimus ethyl acetate fraction against induced T2D in Drosophila larvae. The antidiabetic activity may be attributed to flavonoids, which are the main components of the A. halimus ethyl acetate fraction.
Topics: Animals; Drosophila melanogaster; Hypoglycemic Agents; Acetates; Atriplex; Diabetes Mellitus, Type 2; Plant Extracts; Larva
PubMed: 38623920
DOI: 10.1002/jez.2812 -
Georgian Medical News Feb 2024It was already known that mirabegron, a β3-adrenoceptor agonist, affected cardiac muscle, data also demonstrated that mirabegron induced a relaxant effect in rat aortic...
It was already known that mirabegron, a β3-adrenoceptor agonist, affected cardiac muscle, data also demonstrated that mirabegron induced a relaxant effect in rat aortic vessels by a mechanism dependent on nitric oxide production. This study examined the possible effects of mirabegron on the coronary vascular tone. Results show that mirabegron induced an acute relaxant effect on coronary segments' contractility, and the relaxation is partly dependent on nitric oxide and K+ channel activation. These findings emphasize the need to consider these mechanisms when translating mirabegron's effects to clinical applications. Mirabegron, the first approved β3-adrenoceptor agonist, has demonstrated positive effects in heart failure. Research indicates that β3 agonists induce prompt relaxation in rat aortic and human coronary vessels through a pathway mediated by NO. This study examined mirabegron's influence on bovine coronary segments' contractility. Using isolated tissue baths, the impact of mirabegron on bovine coronary artery segments' contractility was assessed. The plasma level of NO was measured with a specialized kit. NO was determined by measuring plasma nitrite concentrations by spectrophotometric analysis at 540 nm. Mirabegron evoked relaxation in bovine coronary artery segments in a dose-dependent manner. However, this effect was inhibited by the presence of potassium chloride (KCl) (70mM) and methylene blue (30μM). Both potassium channel and NO pathways were found to play a role in the relaxations induced by mirabegron. Furthermore, mirabegron was observed to enhance in vivo nitric oxide (NO) levels, a crucial signaling molecule maintaining cardiovascular equilibrium. Our findings illustrate that mirabegron induces coronary vessel relaxation through the activation of both NO and K+ channels. These findings emphasize the need to consider these mechanisms when translating mirabegron's effects to clinical applications.
Topics: Humans; Cattle; Animals; Rats; Nitric Oxide; Heart; Coronary Vessels; Receptors, Adrenergic; Acetanilides; Thiazoles
PubMed: 38609120
DOI: No ID Found -
Natural Product Research Apr 2024One new compound 3f-[(7',8')-4,4'-dihydroxy-5-methoxy-2,7'-cycloligna-7-en-9-methoxycarbonyl, 9'-carbonyl--] -- D-fructofuranosyl- (→)-- D-glucopyranoside)...
One new compound 3f-[(7',8')-4,4'-dihydroxy-5-methoxy-2,7'-cycloligna-7-en-9-methoxycarbonyl, 9'-carbonyl--] -- D-fructofuranosyl- (→)-- D-glucopyranoside) (Moltkiopsin A) was isolated and identified from the extract of aerial parts of the wild Egyptian plant (Frossk.), family Boraginaceae, for the first time, along with two aryldihydronaphthalene lignans 3f→9':6f→9-[(7',8')-4,4'- dihydroxy-3,3',5-trimethoxy-2,7'-cycloligna-7-en-9,9'-dicarbonyl]-6g-acetyl--D-gluco pyranosyl-(→) --D-fructofuranoside (Trigonotin A) which was reported for the first time from this plant species and a known compound 3f→9':6f→9-[(7',8')-4,4'- dihydroxy-3,3',5-trimethoxy-2,7'-cycloligna-7-en-9,9'-dicarbonyl]--D-gluco pyranosyl - (→)- -D- fructofuranoside (Trigonotin C) . These compounds were separated and purified using different chromatographic techniques and their structures were elucidated by extensive 1D (H and C NMR), and 2D NMR (COSY, HSQC, and HMBC), besides ESI-MS spectral methods. Extracts were screened as antioxidant, antitumor and antibacterial. The different extracts showed moderate to strong antioxidant capacities in DPPH assays. Ethyl acetate, methylene chloride and crude methanol extracts exhibited the most significant free radicals scavenging activity when compared to the standard antioxidant vitamin C. Hexane and butanol fractions showed the highest cytotoxicity against the cancer cell lines HepG2 and MCF-7.
PubMed: 38600852
DOI: 10.1080/14786419.2024.2341307 -
Journal of Inorganic Biochemistry Jun 2024Our prior studies have illustrated that the uracil ruthenium(II) diimino complex, [Ru(Hucp)Cl(PPh)] (1)...
Our prior studies have illustrated that the uracil ruthenium(II) diimino complex, [Ru(Hucp)Cl(PPh)] (1) (Hucp = 2,6-bis-((6-amino-1,3-dimethyluracilimino)methylene)pyridine) displayed high hypoglycemic effects in diet-induced diabetic rats. To rationalize the anti-diabetic effects of 1, three new derivatives have been prepared, cis-[Ru(bpy)(urdp)]Cl (2) (urdp = 2,6-bis-((uracilimino)methylene)pyridine), trans-[RuCl(PPh)(urdp)] (3), and cis-[Ru(bpy)(Hucp)](PF) (4). Various physicochemical techniques were utilized to characterize the structures of the novel ruthenium compounds. Prior to biomolecular interactions or in vitro studies, the stabilities of 1-4 were monitored in anhydrous DMSO, aqueous phosphate buffer containing 2% DMSO, and dichloromethane (DCM) via UV-Vis spectrophotometry. Time-dependent stability studies showed ligand exchange between DMSO nucleophiles and chloride co-ligands of 1 and 3, which was suppressed in the presence of an excess amount of chloride ions. In addition, the metal complexes 1 and 3 are stable in both DCM and an aqueous phosphate buffer containing 2% DMSO. In the case of compounds 2 and 4 with no chloride co-ligands within their coordination spheres, high stability in aqueous phosphate buffer containing 2% DMSO was observed. Fluorescence emission titrations of the individual ruthenium compounds with bovine serum albumin (BSA) showed that the metal compounds interact non-discriminately within the protein's hydrophobic cavities as moderate to strong binders. The metal complexes were capable of disintegrating mature amylin amyloid fibrils. In vivo glucose metabolism studies in liver (Chang) cell lines confirmed enhanced glucose metabolism as evidenced by the increased glucose utilization and glycogen synthesis in liver cell lines in the presence of complexes 2-4.
Topics: Rats; Animals; Coordination Complexes; Serum Albumin, Bovine; Ruthenium; Dimethyl Sulfoxide; Hypoglycemic Agents; Chlorides; Diabetes Mellitus, Experimental; Pyridines; Peptides; Ruthenium Compounds; Glucose; Phosphates; Antineoplastic Agents; Ligands
PubMed: 38554578
DOI: 10.1016/j.jinorgbio.2024.112541 -
Chemistry, An Asian Journal May 2024Terephthalaldehyde-triaminoguanidium chloride covalent organic polymer, Te-TG COP can facilely be incorporated with NaNO by sonication. Te-TG COP incorporated with NaNO...
Terephthalaldehyde-triaminoguanidium chloride covalent organic polymer, Te-TG COP can facilely be incorporated with NaNO by sonication. Te-TG COP incorporated with NaNO via ultrasonication adsorbs Methylene Blue (MB) dye. Te-TG COP alone shows negligible adsorption capacity for MB, however, when treated with NaNO, its adsorption capacity emerges slightly. Moreover, ultrasonication of the NaNO treated COP, Te-TG-NaNO COP shows dramatic increase in its adsorption capacity for MB (q for Te-TG COP ≈0 mg g; for Te-TG-NaNO=17.65 mg g). Emergence of MB dye adsorption property in Te-TG-NaNO COP composite may be attributed primarily to the electrostatic interaction of MB dye molecules with nitrate anions and the sonochemical effect caused fibrous morphological structure of the adsorbent material. The kinetics of MB dye adsorption onto Te-TG-NaNO COP composite exhibits an excellent fit for the pseudo-second order model, suggesting the rate-determining step to be chemisorption. Homogeneous monolayer adsorption of MB dye onto Te-TG-NaNO COP composite can be suggested as the Langmuir isotherm model seemed to be fitted well.
PubMed: 38533850
DOI: 10.1002/asia.202400046 -
Expert Review of Endocrinology &... Jul 2024The effects of pomegranate juice (PJ) and its components on uterine smooth muscle are unknown. Hence, this study unequivocally demonstrates that pomegranate juice (PJ)...
BACKGROUND
The effects of pomegranate juice (PJ) and its components on uterine smooth muscle are unknown. Hence, this study unequivocally demonstrates that pomegranate juice (PJ) significantly impacts myometrial function, providing crucial insights into its relaxant properties and their potential therapeutic applications for uterine-related disorders.
RESEARCH DESIGN AND METHODS
Rat uterine smooth muscle horn strips were suspended in Krebs solution organ baths. Contractions were measured isometrically using a transducer (AD instrument Australia). The effects of PJ were evaluated on contractile activity elicited by potassium chloride (KCl 60 Mm) depolarization. Inhibitors of nitric oxide (L-NAME 3 X 10), guanylate cyclase (methylene blue 1 X 10), and Prostaglandin I (indomethacin 3 X 10), as well as Potassium Channels blockers, were determined.
RESULTS
The juice at concentrations from 1.5-5 mg/ml significantly decreased the rat uterine horn contraction induced by KCl. The NO, cGMP, and PGI inhibitors did not block the relaxation response. Furthermore, the PGI inhibitor significantly enhanced the relaxation effects; K channel blockers had no inhibitory effects on the relaxation responses. Contrarily, GLIB improved considerably relaxation.
CONCLUSION
Research suggests pomegranate juice's active ingredient may reduce uterine contractions and treat uterotonic disorders, potentially preventing preterm birth and dysmenorrhea. Further research is needed to determine its mechanism of action.
TRIAL REGISTRATION
Code: AEC-013.
Topics: Female; Animals; Rats; Fruit and Vegetable Juices; Pomegranate; Uterine Contraction; Muscle Relaxation; Myometrium; Rats, Sprague-Dawley; Uterus; Potassium Chloride; Nitric Oxide; Indomethacin
PubMed: 38525817
DOI: 10.1080/17446651.2024.2334218